2.Pathology of mammary mucinous carcinoma and its molecular markers.
Wei ZHANG ; Yun NIU ; Tong-xian ZHANG ; Shan LIU ; Fen LIU
Chinese Journal of Pathology 2013;42(11):777-779
Adenocarcinoma, Mucinous
;
classification
;
metabolism
;
pathology
;
Biomarkers, Tumor
;
metabolism
;
Breast Neoplasms
;
classification
;
metabolism
;
pathology
;
Carcinoma, Ductal, Breast
;
pathology
;
Carcinoma, Intraductal, Noninfiltrating
;
pathology
;
Female
;
Humans
;
Lymphatic Metastasis
;
Mucin-1
;
metabolism
;
Mucin-2
;
metabolism
;
Mucins
;
metabolism
;
WT1 Proteins
;
metabolism
3.Study on preparation of salvianolic acid phospholipid compound.
Xiao-Yun CHEN ; Zhen-Hai ZHANG ; Dan LIU ; Dan-Hong YU ; E SUN ; Xiao-Bin JIA
China Journal of Chinese Materia Medica 2014;39(2):216-221
To prepare salvianolic acid phospholipid compound. With the compound of salvianolic acids and soybean phospholipid as the index, mono-factor experiment and orthogonal design experiment were conducted to screen its technical parameters. According to the results, the optimal preparation conditions of salvianolic acid phospholipid compound were that THF were taken as the reaction solvent, the concentration time was 3 h, the reactant concentration was 5 g x L(-1), the mass ratio of salvianolic acids and phospholipid was 1: 1.5, and the reaction temperature was 40 degrees C. The oil/water partition coefficient of the prepared salvianolic acid phospholipid compound significant increased in water and buffers with different pH values. The results of phase analysis such as DSC, XRD and FTIR indicated that salvianolic acids existed in phospholipid in an amorphous state.
Alkenes
;
chemistry
;
metabolism
;
Chemical Phenomena
;
Chemistry, Pharmaceutical
;
methods
;
Intestinal Absorption
;
Phospholipids
;
chemistry
;
Polyphenols
;
chemistry
;
metabolism
;
Soybeans
;
chemistry
;
Temperature
4.Study on oral absorption enhancers of astragalus polysaccharides.
Xiao-Yun CHEN ; Xiao-Bin TAN ; E SUN ; Dan LIU ; Xiao-Bin JIA ; Zhen-Hai ZHANG
China Journal of Chinese Materia Medica 2014;39(7):1243-1247
Astragalus polysaccharides was lounded to 4-(2-aminoethylphenol), followed by labeling the APS-Tyr with fluorescein-5-isothiocyanate (FITC) at the secondary amino group. The absorption enhancement effects of low molecular weight chitosan and protamine on astragalus polysaccharides were evaluated via Caco-2 cell culture model. The results show that the fluorecent labeling compound has good stability and high sensitivity. On the other hand low molecular weight chitosan and protamine also can promoted absorption of the astragalus polysaccharides without any cytotoxity, and the absorption increase was more significant with increasing the amount of low molecular weight chitosan and protamine. At the same time, the low molecular weight chitosan has slightly better effect. The transepithelial electric resistance (TEER) of Caco-2 cells show that absorption enhancers could improve its membrane transport permeability by opening tight junctions between cells and increasing the cell membrane fluidity.
Absorption
;
Astragalus Plant
;
chemistry
;
Biological Transport
;
Caco-2 Cells
;
Fluorescein-5-isothiocyanate
;
chemistry
;
Fluorescent Dyes
;
chemistry
;
Humans
;
Plant Extracts
;
chemistry
;
pharmacokinetics
;
Polysaccharides
;
chemistry
;
pharmacokinetics
5.Studies on hydroxyapatite applicatied in coprecipitate of total salvianolic acids phospholipid complex.
Xiao-Yun CHEN ; Zhen-Hai ZHANG ; Dan LIU ; E SUN ; Xiao-Bin JIA
China Journal of Chinese Materia Medica 2014;39(6):992-996
The purpose of this research was to prepare total salvianolic acids-phytosome-HA coprecipitate to improve drug dissolution and its micromeritic properties. Firstly, the coprecipitate was prepared by solvent method and in vitro dissolution of tripterine was performed with the salvianolic acid B and danshensu as criteria. At the same time, the micromeritic properties was characterizated, the structure of samples was characterized by TEM, DSC, XRD and FTIR. Results showed that when the ratio of drug to HA was 1:2, it had a better dissolution, the accumulative drug-release percent in vitro at 60 min was over 90%. At the same time, it has good liquidity and low moisture absorption. Its micromeritic properties have improved. It is proved that the drug still existed amorphously by microstructure analysis. The preparation process is simple and feasible, it has practical value.
Alkenes
;
chemistry
;
Chemical Precipitation
;
Chemistry, Pharmaceutical
;
methods
;
Durapatite
;
chemistry
;
Phospholipids
;
chemistry
;
Polyphenols
;
chemistry
;
Time Factors
6.Research progress of relationship between HMGA2 and tumors.
Chinese Journal of Pathology 2014;43(1):65-67
Animals
;
Apoptosis
;
Biomarkers, Tumor
;
metabolism
;
Cell Cycle
;
Cell Proliferation
;
Epithelial-Mesenchymal Transition
;
HMGA2 Protein
;
genetics
;
metabolism
;
physiology
;
Humans
;
Lymphatic Metastasis
;
Neoplasm Invasiveness
;
Neoplasms
;
blood supply
;
metabolism
;
pathology
;
Neovascularization, Pathologic
7.Secretory breast cancer in a 15-year-old boy: report of a case.
Yun DONG ; Ling-ling GUO ; Feng LIU ; Feng LI
Chinese Journal of Pathology 2013;42(11):768-769
Adenocarcinoma, Mucinous
;
pathology
;
Adenoma
;
pathology
;
Adolescent
;
Breast Neoplasms, Male
;
metabolism
;
pathology
;
secretion
;
surgery
;
Carcinoma
;
metabolism
;
pathology
;
secretion
;
surgery
;
Diagnosis, Differential
;
Humans
;
Keratin-18
;
metabolism
;
Keratin-8
;
metabolism
;
Male
;
Mastectomy, Modified Radical
;
Proto-Oncogene Proteins c-kit
;
metabolism
;
S100 Proteins
;
metabolism
9.Effect of LY294002 and its combination with chemotherapy drugs on the proliferation of human leukemia K562 cell line and its possible mechanism
Ye ZHANG ; Xiu-Juan QU ; Yun-Peng LIU ; Wei JING ; Ke-Zuo HOU ; Yue-E TENG ; Jing-Dong ZHANG ;
China Oncology 2006;0(11):-
Background and purpose:Remarkable advances have been made in cancer chemotherapy by developing new anticancer drugs and therapy strategies.However,multidrug resistance in human cancers remains a major clinical challenge for cancer treatment.Attempts in several clinical studies to reverse multidrug resistance protein (MDR) by using MDR modulators have not yet generated promising results.Our aim was to explored the mechanism of reversal of multidrug resistance in human leukemia K562 cells by PI3-K inhibitor.Methods:Trypanblue dye exclusion method was used to observe the drug sensitivity and the effect of LY294002 on the drug resistance.Western blot to analyze P-gp and p-Akt phenotypes,and flow cytometer was used to measure the intracellular drug accumulation. Results:K562/D induced by DNR was cross resistant to DNR,ADR,VCR and VP16 (Resistance Index:65,52,134 and 50 respectively).DNR induced over-expressions of P-gp and p-Akt in K562/D cells;LY294002 increased the intracellular drug accumulation,and then reversed the drug resistance to DNR,ADR,VCR and VPI6 in K562/D cells(Resistance Index:23,21,63 and 29 respectively),but not in the sensitive cells (K562/S).Conclusion:The multidrug resistance of K562/D cells can be induced by DNR which is related to the P-gp and p-Akt over-expressions, and LY294002 can reverse multidrug resistance in human leukemia cells in vitro via inhibits PI3-K/Akt pathway.
10.Clinical analysis of pneumomediastinum or pneumothorax during the removal of brochial foreign bodies.
Yao-yun TANG ; Jian-yun XIAO ; Su-ping ZHAO ; Ji-wei LIU ; Xiang-e TIAN ; Jing XU
Journal of Central South University(Medical Sciences) 2006;31(3):441-443
OBJECTIVE:
To discuss the etiology, diagnosis, treatment, and prevention of pneumomediastinum or pneumothorax during the removal of bronchial foreign bodies in children.
METHODS:
We analyzed the clinical data of 10 cases of pneumomediastinum or pneumothorax during the removal of bronchial foreign bodies in children.
RESULTS:
Two patients died and the other 8 were cured.
CONCLUSION
Pneumomediastinum or pneumothorax is mainly caused by the intrapulmonary hyper-pressure and fracture of pulmonary bubbles. The prognosis of pneumomediastinum or pneumothorax is closely related to such factors as correct and punctual diagnosis and quick removal of the airway obstruction.
Bronchi
;
Bronchoscopy
;
adverse effects
;
Child, Preschool
;
Female
;
Foreign Bodies
;
surgery
;
Humans
;
Infant
;
Male
;
Mediastinal Emphysema
;
etiology
;
Pneumothorax
;
etiology