Objective:To study the clinical significance of central electric-fetal monitoring system for ante-partum observation and measurement.Methods:The experimental group (253 pregnant women who delivered in our hospital from January,2001 to August,2003) was monitored with the central electric fetal monitoring system.The control group (250 pregnant women who delivered the children in our hospital from July,1998 to September,2000) was not monitered with this system.Then,the results of fetal monitoring and the outcome of prenatal fetuses of the two groups were compared and analyzed.Results:The rate of abnormal FHR was markedly higher in experiment group(30.8%) than in the control group(17.2%),and the rate of variable deceleration in abnormal graph was markedly higher in the experimental group (28.8%) than in the control group (12.4%).There was a significant difference is significant between the two groups.The incidence of asphyxia among newborns was lower in the experimental group (4%) than in the control group (8.4%),and the difference is significant between the two groups.However,there was no significant difference in the rate of cesarean delivery and Simpson forceps delivery between the two groups.Conclusion:Ante-partum monitoring assisted by central electric-fetal monitoring system can improve the prenatal prognosis and increase the rate of cesarean delivery.

Objective:To investigate the sexual concept and sex status of pregnant women so as to instruct pregnant health care and improve living quality.Methods:112 random sample candidates who have mature fetus for delivery in hospital were chosen to investigate and analyze by means of self-edited questionnaires and Eysenck protracted sexual latitude scale test.Results:Both sex frequency and sex continuing time of pregnant women reduced remarkably;their knowledge on sex related to pregnancy was generally poor;average value of Eysenck protracted sexual latitude scale test was 5.25,lying below the average points;professionals and individual professionals have statistics differences in occupation team;the single-mode of sexual intercourse in the sex of pregnant women has the highest percentage.Conclusion:Pregnant women lack the related health care knowledge on sex,and their concept of sex is comparatively conservative,so they can not enjoy full sex and need further improvement.

Objective:Under the background that the army hospitals need to employ civilian nurses in a large quantity,we try to grope a scientific,objective method to choose the nurse who matches post.Methods:Referring to related data and the ritual of talented person evaluation,we chose the evaluation methods including paper test,computer test,personal interview and data integration;arranged the steps of planning,assigning the weight,performing evaluation,integrating the test results,and tracking for feedback;in order to form the mode to test the employed nurses' occupational diathesis.Results:We fulfilled a series of scales for mood and hobby,weight score,quality structure,evaluation method comparison and nurse's professional quality evaluation,etc,and determined the evaluation methods and procedures.Conclusion:Evaluating more than 100 nurses who are newly employed using this new mode,the result demonstrates that this evaluation mode can be easily operated,and is a guide in employing quantities of professional nurses for a hospital.

Objective To construct a cDNA expression library of Humulus pollen and provide the basis for screening the major allergenic components and producing recombinant allergen of Humulus pollen.Methods Verdure Humulus pollen were collected and preserved in liquid nitrogen after being sift out.Total RNA was extracted from the Humulus pollen with trizol reagent,and cDNA was synthesized by RT-PCR with purified total RNA.Then the cDNA was digested by SfiⅠ and the fragments smaller than 400bp were removed by chroma spin-400 column,and the fragments longer than 400bp were ligated with ?TripIEx2 Vector.The library was packaged in vitro and a small portion of packaged phage was used to infect E.coli XL1-Blue MRF′ for titration.The diversity of the library and the length of the inserted fragments were analyzed by PCR.Results The cDNA expression library contained 5?10~(5) recombinants and the percentage of recombination was 90%.The average length of inserted cDNA fragments was about 1.02kb.Conclusion The constructed cDNA expression library contains appropriate contents and sizes of cDNA fragments and is qualified for screening target cDNA clone.

AIM: After human lung epithelial cells (A549) infected with respiratory syncytial virus (RSV), inducer cells over-express active transmitter. The study investigated the expression changes in nuclear factor ?B (NF-?B), inducible nitric oxide synthase (iNOS) mRNA and protein, nitric oxide (NO) and malonaldehyde (MDA) on human lung epithelial cells (A549) infected with RSV. METHODS: Experiments were performed at the Laboratory of Etiology and Immunology, Anhui Medical University between December 2006 and May 2007. ① Gyopreserved virus broliferated in Hep-2 cells after recovery. A549 cells infected with RSV in vitro were used to collect cells and cellular culture supernatant at hours 4, 8, 16 and 24. Cells non-infected with RSV were served as controls. ② Reverse transcription-polymerase chain reaction (RT-PCR) was used to evaluate the expression of iNOS mRNA. The expression of iNOS protein and NF-?B was detected by Western-Blot. The concentrations of NO and MDA were measured by nitrate reductase method and thiobarbituric acid method. RESULTS: ①The basal expression of NF-?B was increased with the prolongation of RSV infection to A549 cells. ②RSV infection to A549 increased the amounts of mRNA and protein of iNOS in a time-dependent manner. The expression of iNOS mRNA was about 12 times as many as basal expression. RSV infection promoted the expression of iNOS protein, which was higher than the control group. The activities of NF-?B significantly were positively correlated with the mRNA and protein expression of iNOS. ③RSV could improve A549 cells secreting NO and MDA, but the NO levels rose slowly during the 24 hours of infection. CONCLUSION: The inflammatory response of RSV can increase the activity of NF-?B and it is positive correlated with the increase in iNOS mRNA and protein levels.

Objective To systematically review the effect of dietary and physical activity interventions on weight management during pregnancy.Methods The PubMed (from Jan.1990 to Sep.2013),Springer (from Jan.1960 to Sep.2013),Willy (from Jan.1990 to Sep.2013),Web of Science (from Jan.1990 to Sep.2013),Wanfang data (from Jan.1995 to Sep.2013),China National Knowledge Infrastructure (CNKI) (from Jan.1995 to Sep.2013)were searched for relevant articles.All published randomized controlled trails (RCT) on dietary and physical activity interventions during pregnancy were considered.The quality of RCTs was appraised and data were extracted by 3 reviewers independently.Meta-analysis were conducted by RevMan 5.2 software.Results In total,eleven RCTs were included in this review.Meta-analysis revealed that dietary and physical activity intervention significantly reduced incidence of cesarean section and macrosomia;However,there were no statistically significant differences in the total gestational weight gain.Conclusions Interventions based on dietary and physical activity during pregnancy appears effective in reducing incidence of cesarean section and macrosomia.However,there are many limitation of the Meta analysis,therefore more multi-center,large sample with high quality RCTs are required for future verification.

Animals ; Antineoplastic Agents ; therapeutic use ; Breast Neoplasms ; drug therapy ; genetics ; metabolism ; Cyclin-Dependent Kinase Inhibitor p27 ; Fenretinide ; therapeutic use ; Hepatocyte Nuclear Factor 3-alpha ; genetics ; metabolism ; Humans ; Intracellular Signaling Peptides and Proteins ; metabolism ; Prognosis ; Receptors, Estrogen ; metabolism

Emodin is the main effective monomer of Rhubarb, which has a variety of pharmacological actions and valuable clinical applications.Recently, there are many reports about molecular mechanisms of emodin pharmacological actions,and they givehighly attention on the dephlogisticating and antineoplastic effects. These effects are achieved through affecting the concentration and transportation of ions, resisting of oxidation and free radical, and affecting the secretion of inflammatory factor and the enzyme activity and apoptosis and so on. This article summarizes the reports and hopes to provide theoretical basis for the practical application.

At present scopolamine is the most powerful single anti-motion sickness drug, but with prominent unwanted side effects. Many attempts had been made to decrease the unwanted side effects, but no any approach was considered to be successful. Based on our working hypothesis that central cholinolytic activity of anticholinergics may not be parallel completely to their side effects, a series of alicyclic amino alcohol esters were designed, synthesized and evaluated. One of the best compounds, phencynonate HCl, was obtained by transesteration of methyl α-phenyl-α-cyclopentyl-α-hydroxy acetate with N-methyl-3-azabicyclo(3,3,1)nonan-9α-ol. In animal models it was demonstrated that at equivalent anti-motion sickness dose the side effects of phencynonate were milder than those of two other central anticholinergic anti-motion sickness drugs scopolamine HBr and difenidol HCl. In clinical trials the overall effectiveness rates for prevention of seasickness and carsickness of phencynonate (oral 2-4 mg/person) was very significantly higher than that of placebo, and also significantly higher than that of difenidol (oral 25-50 mg/person). In self controlled rotatory chair experiments in hospital laboratory, the preventive effects of phencynonate and difenidol in reducing the changes in electronystagmus and electrogastrogram were statistically significant. In another self controlled rotation experiment, phencynonate (2-4 mg/person) and scopolamine (0.3-0.6 mg/person) showed significant anti-motion sickness effects in reducing the gastric electric cycles of electrogastrogram and the Graybiel scores of acute motion sickness and significant inhibitory effects on visual-vestibular interaction dose-dependently. The anti-motion sickness effects of phencynonate 2 and 4 mg were correspondent with those of scopolamine 0.3 and 0.6 mg, respectively. Student pilots with high susceptibility to airsickness were stimulated by Coriolis acceleration. The course of desensitization and habituation to airsickness training in phencynonate group (3 mg/person) was significantly shorter than that of placebo. There was no rebounding in sensitivity to Coriolis stimulation after discontinuing phencynonate, which was reported in case of scopolamine. The side effects of phencynonate HCl were mild dry mouth (9.7%) and drowsiness (9.97%). The incidence of drowsiness is significantly lower than that of difenidol. The side effect of drowsiness was only appeared in aboard ship and bus experiments, but not in PhaseⅠ trial in hospital or in laboratory rotation tests. The incidence of drowsiness of phencynonate was also lower than that of dramamine in aboard tank experiment. Phencynonate could effectively control the acute attack of vertigo, especially Meniere′s disease and positional vertigo. In animal models of Parkinson′s disease and parkinsonism, phencynonate showed morepotent antagonistic effects than clinical common used trihexyphendyl. In summary, phencynonate is a new central anticholinergic anti-motion sickness drug with higher efficacy and lower central inhibitory side effect than difenidol and scopolamine in prevention of motion sickness. Phencynonate HCl was approved on Dec 25,1993 by State Food and Drug Administration of China as a Class Ⅰ new drug for the prevention and treatment of motion sickness in the market in China.

DNA repair gene polymorphism can change the functions and efficiency of DNA repair,influence cancer susceptibility.Many studies have been reported that DNA damage repair gene polymorphisms may be related to cancer susceptibility mutation in a variety of tumors and plays an important role in the pathological process.In addition,DNA damage repair genes may interact with other genes,the combined effect of tumor occurrence,development.Lung cancer is the well-studied tumor in this respect.In this paper,DNA damage repair gene XRCC and hOGG1 polymorphisms biological characteristics,these gene single nucleotide polymorphisms as well as cancer susceptibility were reviewed to provide a theoretical basis for the tumor prevention,diagnosis and treatment.