Objective To investigate the infectious state and drug resistance of Haemophilus par lainfluenzae in respiratory tract infection, so as to instruct the rational use of antibiotics. Methods Haemophilus par lainfluenzae were isolated from the pa-tients with respiratory tract infection and performed by K-B methods. Nitrocefin slip test was applied into detecting β-lactamase. Re-sults A total of 45 Hacmophilus par lainfluenzae were isolated from 760 specimen, with the isolation rate being 5.92%. Amoxicil-lin/claulanic acid,Azithromycin, Imipenem,Ceftazidime and Cefuroxime had the better antibiotic function and the average drug re-sistance was lower than 5 % ,The β-lactamase positive rate was 37.78 %. Conclusion The high drug resistance and β-lactamase pos-itive rate of Haemophilus par lainfluenzae isolated from respiratory tract characterized by multi-resistance must be taken seriousely.

This article analyses the chemical test results of several common repair mesh, and concludes the currently several common repair mesh are safe and reliable.
Prostheses and Implants ; Surgical Mesh ; standards

The cognitive conflict between doctors and patients mainly manifests in the different cognition of diseases,the expectation of medical skill and the understanding of medical science.The way to dissolve the cognitive conflict between doctors and patients lies in strengthening exchange and communication between them.

Animals ; Cell Culture Techniques ; Cell Differentiation ; Cell Proliferation ; Embryonic Stem Cells ; cytology ; metabolism ; Germ Cells ; cytology ; metabolism ; Germ Layers ; cytology ; metabolism ; Humans ; Induced Pluripotent Stem Cells ; cytology ; metabolism ; Mice ; Pluripotent Stem Cells ; cytology ; metabolism ; Reproductive Techniques, Assisted

Objective To investigate the differences in expression of hypoxia-inducible factor-1? (HIF-1?) in placental trophoblasts between normal pregnancy and pregnancy-induced hypertension (PIH) and the relationship of HIF-1? expression with magnesium sulfate treatment of PIH. Methods Immunohistochemical streptavidin biotin-peroxidase complex technique (SABC) was used to examine the expression of HIF-1? in 20 cases of PIH and 20 cases of normal placenta. Three groups of PIH were divided according to the degrees of severity. The results were analyzed by computer-assisted imaging and compared by t-test. Results There was significant difference in the expression of HIF-1? between the PIH group and the control group (P

Herbal drug-induced liver injury is a hot issue in the fi eld of TCM researches.Rapid screening and evaluating of herbal drug-induced liver injury have become one of the key techniques of herbal research.This text carried on the discussion on the research status of it from active,toxic ingredient,toxic mechanism and decreasing toxicity of Chinese herbal drugs.At the same time,the text viewed the application foreground of gene chip technique in the study of it.The current status and methods of it was discussed.In addition,most of the reports adopted in this paper were about studies on crude drugs,and discussion on how to correctly treat herbal drug-induced liver injury.

Child ; Diagnosis, Differential ; Diagnostic Errors ; Female ; Humans ; Porphyria, Acute Intermittent ; diagnosis ; therapy

Objective: To investigate the effect of a small interfering RNA (si-RNA) targeting signal transducer and activator of transcription-3 (STAT3) on the biological function of human endometrial cancer cell line HEC-1B. Methods: The HEC-1B cells were transfected with chemically synthesized si-RNA targeting STAT3 (si-STAT3). Then the expression of STAT3 mRNA was detected by real-time fluorescence quantitative-PCR, the proliferation ability was determined by cell counting kit-8 (CCK-8) assay, the cell cycle distribution and apoptosis were measured by flow cytometry, the migration and invasion were examined by Transwell chamber, and the expression levels of STAT3, phospho-STAT3 (p-STAT3), and cyclin D2 (CCND2) and metalloproteinase-2 (MMP-2) were detected by Western blotting. Results: As compared with the blank control group (only liposome was added) or transfection with si-control group, the expression level of STAT3 mRNA in HEC-1B cells after transfection with si-STAT3 was down-regulated (P < 0.01), the proliferation ability was inhibited (P < 0.01), the percentage of the cells in G1-phase was increased and which in S1-phase was decreased (P < 0.01). The abilities of migration and invasion of HEC-1B cells after transfection with si-STAT3 were inhibited (P < 0.01), and the expression levels of STAT3, p-STAT3, CCND2 and MMP-2 proteins were down-regulated (P < 0.01). Conclusion: Si-RNA targeting STAT3 gene can inhibit the expressions of STAT3 mRNA and protein in HEC-1B cells, and also inhibit the cell proliferation, the abilities of migration and invasion, and apoptosis. Copyright © 2013 by TUMOR.

Objective:To investigate the acid resistance of tooth hard tissues irradiated by different power outputs of Er,Cr∶YSGG laser.Methods:Samples in laser groups were irradiated using Er,Cr∶ YSGG laser with the power outputs of 2.5,3.5 and 5 W for enamel and 2 W,3 W and 4 W for dentin,respectively.The calcium and phosphate ion dissolved was measured after decalcification in lactate buffer solution for 24 h.The atomic percentage of calcium and phosphate on the surface of samples was examined by X-ray energy dispersive spectroscopy and the morphological changes were investigated by SEM.Results:In all laser groups of enamel samples except 2.5 W group calcium and phosphate ion dissolved less than in control group and block group (P＜0.05).There's no statistic difference between different power groups.Compared with control group and block group,Ca/P ratio increased(P＜0.05).There's no statistic difference of the atomic percentage of Ca and P on the surface of dentin samples between each 2 groups.SEM observation showed that the surface of the laser irradiated samples was rouph,the space among enamel fibers was increased,the dentin around dentinal tuble orifice was protruded and looked like collar flange.Conclusion:Er,Cr∶YSGG laser irradiation with a range of power is effective in increasing acquired acid resistance of dental hard tissue.There was no relationship between laser power outputs and acid resistance.

Objective To investigate different scoring systems in predicting the severity of acute pancreatitis(AP).Methods The clinical data of 1 56 patients with AP were retrospectively reviewed.Ser-um c-reactive protein(CRP)levels were measuredat admission.According to the Chinese guidelines for the management of acute pancreatitis(2007),all the patients were categorizedas either mild acute pancre-atitis(MAP)or severe acute pancreatitis(SAP).Ranson,acute physiology and chronic health evaluation (APACHE)-Ⅱ,bedside index for severity in acute pancreatitis(BISAP),and computed tomography se-verity index(CTSI)scoring systemswere calculated according to the corresponding grading standardsin all patients.Patients were divided into MAP group(APACHEⅡ <8,Ranson <3,BISAP <2,CTSI <3 and CRP <21 .4)and SAP group (APACHEⅡ≥8,Ranson≥3,BISAP≥2,CTSI≥3 and CRP≥21 .4)ac-cording to the scoring results.ROC curve was used to compare the difference among the systems.Results Among the 1 56 patients,21 (1 3.5%)were classified as SAP and 1 35 as (86.5%)MAP.AUCs for Ranson,BISAP,APACHEⅡ,CTSI,and CRP in predicting SAP were 0.69 (95%CI:0.62-0.76),0.74 (95%CI:0.66-0.80),0.78 (95%CI:0.70-0.84),0.69 (95%CI:0.61 -0.76),and 0.68 (95%CI:0.57-0.78),respectively.There were no significant differences among these scoring systems.Conclusion There were no significant differencesin predicting the severity of AP among these scoring systems. Therefore,the early prediction of SAP should consider multiple scoring systems,and the referential signifi-cance of accessing and applying a simpler laboratory indicator deserves further studies.