The cognitive conflict between doctors and patients mainly manifests in the different cognition of diseases,the expectation of medical skill and the understanding of medical science.The way to dissolve the cognitive conflict between doctors and patients lies in strengthening exchange and communication between them.

This article analyses the chemical test results of several common repair mesh, and concludes the currently several common repair mesh are safe and reliable.
Prostheses and Implants ; Surgical Mesh ; standards

Objective To investigate the infectious state and drug resistance of Haemophilus par lainfluenzae in respiratory tract infection, so as to instruct the rational use of antibiotics. Methods Haemophilus par lainfluenzae were isolated from the pa-tients with respiratory tract infection and performed by K-B methods. Nitrocefin slip test was applied into detecting β-lactamase. Re-sults A total of 45 Hacmophilus par lainfluenzae were isolated from 760 specimen, with the isolation rate being 5.92%. Amoxicil-lin/claulanic acid,Azithromycin, Imipenem,Ceftazidime and Cefuroxime had the better antibiotic function and the average drug re-sistance was lower than 5 % ,The β-lactamase positive rate was 37.78 %. Conclusion The high drug resistance and β-lactamase pos-itive rate of Haemophilus par lainfluenzae isolated from respiratory tract characterized by multi-resistance must be taken seriousely.

Animals ; Cell Culture Techniques ; Cell Differentiation ; Cell Proliferation ; Embryonic Stem Cells ; cytology ; metabolism ; Germ Cells ; cytology ; metabolism ; Germ Layers ; cytology ; metabolism ; Humans ; Induced Pluripotent Stem Cells ; cytology ; metabolism ; Mice ; Pluripotent Stem Cells ; cytology ; metabolism ; Reproductive Techniques, Assisted

Herbal drug-induced liver injury is a hot issue in the fi eld of TCM researches.Rapid screening and evaluating of herbal drug-induced liver injury have become one of the key techniques of herbal research.This text carried on the discussion on the research status of it from active,toxic ingredient,toxic mechanism and decreasing toxicity of Chinese herbal drugs.At the same time,the text viewed the application foreground of gene chip technique in the study of it.The current status and methods of it was discussed.In addition,most of the reports adopted in this paper were about studies on crude drugs,and discussion on how to correctly treat herbal drug-induced liver injury.

Objective To investigate the differences in expression of hypoxia-inducible factor-1? (HIF-1?) in placental trophoblasts between normal pregnancy and pregnancy-induced hypertension (PIH) and the relationship of HIF-1? expression with magnesium sulfate treatment of PIH. Methods Immunohistochemical streptavidin biotin-peroxidase complex technique (SABC) was used to examine the expression of HIF-1? in 20 cases of PIH and 20 cases of normal placenta. Three groups of PIH were divided according to the degrees of severity. The results were analyzed by computer-assisted imaging and compared by t-test. Results There was significant difference in the expression of HIF-1? between the PIH group and the control group (P

Objective: To investigate the effect of a small interfering RNA (si-RNA) targeting signal transducer and activator of transcription-3 (STAT3) on the biological function of human endometrial cancer cell line HEC-1B. Methods: The HEC-1B cells were transfected with chemically synthesized si-RNA targeting STAT3 (si-STAT3). Then the expression of STAT3 mRNA was detected by real-time fluorescence quantitative-PCR, the proliferation ability was determined by cell counting kit-8 (CCK-8) assay, the cell cycle distribution and apoptosis were measured by flow cytometry, the migration and invasion were examined by Transwell chamber, and the expression levels of STAT3, phospho-STAT3 (p-STAT3), and cyclin D2 (CCND2) and metalloproteinase-2 (MMP-2) were detected by Western blotting. Results: As compared with the blank control group (only liposome was added) or transfection with si-control group, the expression level of STAT3 mRNA in HEC-1B cells after transfection with si-STAT3 was down-regulated (P < 0.01), the proliferation ability was inhibited (P < 0.01), the percentage of the cells in G1-phase was increased and which in S1-phase was decreased (P < 0.01). The abilities of migration and invasion of HEC-1B cells after transfection with si-STAT3 were inhibited (P < 0.01), and the expression levels of STAT3, p-STAT3, CCND2 and MMP-2 proteins were down-regulated (P < 0.01). Conclusion: Si-RNA targeting STAT3 gene can inhibit the expressions of STAT3 mRNA and protein in HEC-1B cells, and also inhibit the cell proliferation, the abilities of migration and invasion, and apoptosis. Copyright © 2013 by TUMOR.

Child ; Diagnosis, Differential ; Diagnostic Errors ; Female ; Humans ; Porphyria, Acute Intermittent ; diagnosis ; therapy

Objective To investigate the effect of steroid hormones on lymphatic stomata of ovarian bursa and fluid adsorption. Methods The lymphatic adsorption was traced by trypan blue,and the ultrastructure of the lymphatic stomata and estrogen receptor level of mesothelial cells on ovarian bursa was observed by scanning electron microscopy,transmission electron microscopy and immuno-colloid golden technology. Results The absorption and opening area of lymphatic stomata were enhanced in estrogen group mice,and decreased in androgen group as compared to control group mice.Estrogen receptor was first identified in the nuclei of mesothelial cells on the inner layer of mice ovarian bursa and the level was down-regulated by estrogen and slightly up-regulated by androgen.Conclusion The absorption and the opening area of lymphatic stomata can be regulated by steroid hormones in mice,and the machanism may be related to the expression levels of estrogen receptor in the nuclei of mesothelial cells.

Carotid stenosis is one of the important causes of cerebral infarction. It has been demonstrated that carotid angioplasty and stenting (CAS) can prevent the occurrence of stroke, and its clinical application is continuously increasing. Although CAS is a microinvasive technique, it has some potential complications, such as hemodynamic abnormalities, hyperperfusion syndrome, cerebral infarction, and restenosis. This article reviews the complications of CAS and their management strategies.