1. Deferoxamine preconditioning protects against hypoxia injury in astrocytes
Academic Journal of Second Military Medical University 2010;31(2):144-147
Objective: To investigate the protective effect of deferoxamine preconditioning against hypoxia injury in astrocytes and the underlying mechanisms. Methods: Astrocytes were cultured under ischemia stress, which was mimicked by oxygen and glucose deprivation (OGD) with deferoxamine. Astrocytes were divided into three groups: normally cultured group; Deferoxamine pretreated group: astrocytes were pretreated with Deferoxamine and then treated with Deferoxamine OGD; and OGD group; astrocytes were treated with Deferoxamine OGD. The cell viability examination, the ratio of condensed nuclei, and the morphological changes were used to assess the protective effect of deferoxamine. The expression of HIF-1a and EPO protein and mRNA in astrocytes was examined by immunofluorescence staining and RT-PCR, respectively. Results: The morphology of AS in the deferoxamine pretreated group kept intact. AS viability in deferoxamine pretreated group was 58% of the normally cultured group, and that in the OGD group was 25% of the normally cultured group (P<0. 05). The ratio of condensed nuclei in deferoxamine pretreated group was 38% and that in OGD group was 30% (P<0. 05). Immunofluorescence staining found that expression HIF-1a, and EPO protein appeared in cultured astrocytes after deferoxamine pretreatment. RT-PCR confirmed that mRNA of HIF-1a and EPO was up-regulated after deferoxamine pretreatment. Conclusion: Deferoxamine preconditioning can protect the astrocytes from hypoxia damage, which is related to deferoxamine-induced increase of HIF-1a and EPO expression.
2.Clinical analysis of cognitive function and depressive states in patients with age-related macular degeneration
Na, LI ; Yun-Xia, XU ; Xin, YAN
International Eye Science 2017;17(10):1905-1907
AIM: To investigate the difference of cognitive impairment and depression between age-related macular degeneration ( AMD ) group and the control group patients. ·METHODS: A prospective case-control study was performed from November 2014 to August 2016 in the hospital for AMD patients and sex-matched control group. The Mini-Mental State Examination ( MMSE) and the Geriatric Depression Scale ( GDS ) score of each patient were collected for statistical analyzing. ·RESULTS: There were total 84 cases ( 168 eyes ) included in the study. The difference of visual acuity between the two group was statistically significant ( F=8. 953, P=0. 004) by baseline data analyzing. There were no significant differences in MMSE scores between the two groups according to educational status ( P>0. 05 ) , while the prevalence of cognitive impairment in each group was statistically significant (x2 =4. 14, P=0. 042). The difference of GDS scores, prevalence of total and mild depression between two groups were both statistically significant (F=5. 852, P=0. 018; x2=6. 372, P=0. 012; x2 = 5. 674, P = 0. 017 ). However, there was no statistically significant difference in the prevalence of moderate to severe depression (x2=0. 672, P=0. 412). ·CONCLUSION: AMD patients have a higher prevalence of depression. Although MMSE score differences were not statistically significant in subgroup analysis by educational levels, AMD patients are more likely to have cognitive impairment overall.
3.Bacterial culture of vaginal discharge and their resistance to antibiotics
Yun LI ; Zhongtao LI ; Zhengwu XIA ; Liang QU
Chinese Journal of Postgraduates of Medicine 2006;0(15):-
Objective To investigate the distribution of pathogenic bacteria of vaginal discharge in bacterial vaginosis.Methods The results of bacterial culture and drug sensitive tests of vaginal discharge from patients with bacterial vaginosis were analyzed.Results The positive rate of bacteria culture of vaginal discharge was 79.3%(115/145).The dominant bacteria were staphylococcus epidermidis 27.0%(31/115),staphylococcus intermedius and staphylococcus aureus 13.0%(15/115),which were obviously higher than other germs.The drug sensitive tests showed that staphylococcus were relatively sensitive to vancomycin,fosfomycin,amikacin and rifampin.But the drug resistance to penicillin,tetracycline,erythromycin and oxacillin was the highest.Conclusion The kinds of pathogenic bacteria in vaginal discharge are various.The main bacterium is staphylococcus,and drug resistance is very severe.The isolation and drug sensitive test of pathogenic bacteria play an important role in diagnosis and treatment of gynecological disease.
4.Quality classification standard of Scrophularia ningpoensis seedlings.
Xue ZHANG ; Da-xia CHEN ; Jun TAN ; Long-yun LI
China Journal of Chinese Materia Medica 2015;40(6):1079-1085
The morphological indexes of the Scuophularia ningpoensis seedlings including the longth, diameter and weight were measured, clustering analysis was used to set up the standard quality grading of seedlings of S. Ningpoensis by SPSS. Field experiment was carried out to measure the indicators of plants growth and development, the yield and the quality. The results showed that the growth and yield of class I seedlings were better than those of class II and III. The content of main active ingredients was affected barely by seedlings classification. To ensure the quality, class II seedlings or above should be used for plantation. The established quality classification standard of S. ningpoensis seedlings was scientific and feasible, and provides the basis for the standardized cultivation of S. ningpoensis.
Drugs, Chinese Herbal
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analysis
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standards
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Quality Control
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Scrophularia
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chemistry
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classification
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growth & development
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Seedlings
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chemistry
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growth & development
6.Dectection of p16~(CDKN2A) exon 1 methylation in experimental tongue carcinogenesis in rats
Bin CHENG ; Yun HONG ; Chunyang LI ; Hongbin XIA ; Linglan YANG
Journal of Practical Stomatology 1995;0(04):-
Objective: To detect methylation status of p16 CDKN2A exon 1 during experimental carcinogenesis in rats. Methods:Thirty male clean SD rats were fed with 0.02 g/L of 4-nitroquinoline-oxide (4NQO) in drinking water.13, 16 and 24 weeks after experiment the normal, moderate-severe dysplasia and invasive squamous cell carcinoma tissues were removed from their tongues respectively; then the methylation status of p16 CDKN2A exon 1 were detected by methylation-specific PCR(MSP). Results:A 123 bp-unmethylated product was amplified in all samples but the methylated product was not detected in any of the samples. Conclusion: The p16 CDKN2A exon 1 appeares to be unmethylated during carcinogenesis of tongue cancer in experimental rats.
7.Research on design of hospital inspection information system and HIS interface
Li-xia HUANGFU ; Yun-hong LU ; Ming GONG
China Medical Equipment 2015;(12):61-63
Objective:To develop an interface program between a hospital inspection information system and Hospital Information System(HIS). Such interface program not only guarantees the safety and independence of HIS, but achieves interoperability between HIS and other third-party system.Methods: The interface program between the inspection information system and HIS conforming to hospital demands is developed by using Visual Studio 2008 development platform and WebServices+XML technology.Results: The interface between the inspection information system and HIS operates stably, reliably and efficiently, avoiding manually input information errors, reducing the amount of registration work, and raising work efficiency.Conclusion:WebServices+XML technology is utilized to develop an interface characterized by safety, stability and reliable performance, realizing resource sharing among information systems in the hospital. Besides, the interface can be expanded in the future to adapt to other third-party system, achieving customization.
8.Effect of leptin on proliferation and c-myc gene expression of human colon carcinoma HT-29 cell line
Chunying LIU ; Mingqiang LI ; Qinggong YU ; Min SHU ; Yun XIA
Journal of Chinese Physician 2009;11(1):70-72
Objective To detect the effect of leptin on proliferation and c.Myc mRNA expression of human colon carcinoma HT-29 cell line and investigate the role of Leptin in the development of the HT-29 cell line.Methods Human colon carcinoma HT-29 cell line was cultured in vitro.After treatment with various concentration of Leptin for 72h.MTr was used to detect the proliferative and inhibitive status.And c-myc mRNA-expression Was detected by RT-PCR.Results After treatment with various concentration of Leptin.The cell pmlifemtion and c-myc mRNA expression Wag obviously promoted,compared with the control group.The effect wag in a time-dose-dependent manner within a certain range.Conclusion Leptin can improve cell proliferation and c-myc gene expression level in HT-29 cell line.Promoting the c-myc gene expression level may be one of the reasons that Leptin improves the proliferation of the human colon carcinoma HT-29 cell line.
9.Quality Criteria of Lianqi Bushen Capsule
Jiwei XIA ; Youcai WAN ; Yun LI ; Daoqing LIU
China Pharmacy 2001;0(07):-
OBJECTIVE:To establish the quality standard of Lianqi bushen capsule.METHODS: TLC was employed to identify Panax ginseng in Lianqi bushen capsule,while the content of trigonelline was determined by RP-HPLC.The determination was performed on Kromasil C18(150 mm?4.6 mm,5 ?m)column and mobile phase consisted of methanol-0.05%sodium dodecyl sulfonate-acetic acid(20 ∶ 80 ∶ 0.1)with flow rate of 1 mL?min-1.The detection wavelength was set at 265 nm and column temperature was set at 30 ℃.RESULTS:P.ginseng can be identified by TLC.The linear range of trigonelline was 10~200 ?g?mL-1(r=0.999 9) and average recovery was 98.86%(RSD=1.4%,n=5).CONCLUSION:The standard is used for the quality control of Lianqi bushen capsule.
10.Effect of levosimendan on SDNN and SDANN changes in patients with acute decompensated heart failure and its clinical efficacy and safety
Mingying LI ; Yun DING ; Yanfei WU ; Liang XIA
Chinese Journal of Biochemical Pharmaceutics 2017;37(5):246-248
Objective To investigate the therapeutic effect of levosimendan on acute decompensated heart failure and its effect on SDNN and SDANN values.MethodsThe clinical data of patients with acute decompensated heart failure treated in Zhejiang Greentown Cardiovascular Hospital from January 2014 to August 2016 were retrospectively analyzed.According to their treatment methods, they were divided into control group and observation group.There were 49 cases in the control group and 71 cases in the observation group.The control group was given conventional drug treatment, the observation group was given levosimendan treatment on the basis of the control group.The therapeutic effects of the two groups were observed.The difference of cardiac function, SDNN, SDANN value and adverse reaction between the two groups were compared before and after treatment.ResultsAfter treatment, the observation group efficiency was 98.59%, significantly higher than the control group;the observation group LAD, RAD, LVESD, LVEDD and BNP lower than the control group, the level of LVEF was higher than the control group, the difference was statistically significant (P<0.05);the observation group SDNN, SDANN, RMSSD and PNN50 (%) was higher than that of the control group, the difference was statistically significant (P<0.05).ConclusionLevosimendan has good therapeutic effect on acute decompensated heart failure, can significantly improve the level of SDNN, SDANN, and the incidence of adverse reactions is low.