Objective: To establish a method for the determination of the related substances in pantoprazole sodium capsules. Methods:A Kromasil C18 column (250 mm × 4. 6 mm, 5 μm) was used. 0. 01 mol·L-1 monopotassium phosphate solution (adjus-ting pH to 7. 0 with phosphoric acid)-acetonitrile was adopted as the mobile phase with gradient elution. The detection wavelength was 289 nm and the column temperature was 40℃. The injection volume was 20μl and the flow rate was 1. 0 ml·min-1 . Results:Panto-prazole sodium and its degradation substances could be well separated. The limit of detection and quantification of pantoprazole sodium was 0. 16 ng and 0. 48 ng, respectively. Conclusion:The method is simple, specific and sensitive, and can be applied to determine the related substances in pantoprazole sodium.

Adult ; Chronic Periodontitis ; diagnosis ; diagnostic imaging ; therapy ; Combined Modality Therapy ; Dental Scaling ; Follow-Up Studies ; Furcation Defects ; diagnosis ; diagnostic imaging ; therapy ; Humans ; Male ; Orthodontics, Corrective ; Periodontal Index ; Radiography, Panoramic ; Root Canal Therapy

Objective The questionaires of the ONFH aegers who were hospitalized in Kanggu hospital,during 2005-2008 ,were collect-ed to evaluate the therapeutic effects of acupotomy by proper statistical methods. Methods Four indexes according to the criteria of Therapeutic effect of adult ONFH recovery and reconstruction (ZHAO Dewei, 1996) were measured to evaluate the effect of the treatment. Results Monofac-torial analysis showed that the treatrnent was effective,but some phenomena could not be correctly explined in specialized field. This problem was re-solved appropriately by structural equation model(SEM). Conclusion By the method of SEM, the confounding factors could be better controlled and the evaluation was more reliable.

Ear Diseases ; congenital ; Ear, Middle ; abnormalities ; Humans

Objective To explore the inhibition effect of interleukin-10(IL-10)on pneumonic anaphylacticitis in mice with asthma and its effects on the level of serum IgE.Methods Thirty BALB/c male mice whose weight were(20?2)g were randomly divided into 3 groups:normal group(n=10),asthma model group(n=10)and IL-10 treatment group(n=10).Asthma model group and IL-10 group were sensitized with 1 g?L-1 ovalbumin,then asthma model was made with inhallating 10 g?L-1 VOA 20 mL.The normal group was not administered anything as a negative group.Before inhallating with 10 g?L-1 VOA,asthma model group was administered inhalation with 9 g?L-1 saline 0.1 mL,and IL-10 treatment group was administered inhalation with 0.1 mL IL-10 whose concentration was 0.2 ?g?L-1.The level of IgE was measured by ELISA reagent box,eviscerated lungs after collecting blood and then made them into pathological slices.Results There was an obvious distinction in the mice pathological change of lungs and bronchus between asthma model group and other groups,but no significant difference between IL-10 treatment group and normal group.There was no obvious infiltration of inflammatory cells in the airways of IL-10 treatment group and normal group.However,there were a great number of inflammatory cells in the interstitial and peribronchovascular regions of asthma model group.The levels of serum IgE were(5.219?0.427)?g?L-1,(6.633?0.145)?g?L-1 and(5.503?0.566)?g?L-1 in normal group,asthma model group and IL-10 treatment group,respectively.The level of serum IgE in asthma model group increased obviously compared with normal group and IL-10 treatment group,with significant differences(Pa0.05).Conclusions Inhalation of IL-10 can optimally control the pneumonic anaphylacticitis,improve the situation of airway inflammation in mice asthmatic model and reduce the IgE serum level.

Animals ; Chromatography, High Pressure Liquid ; methods ; Methoxychlor ; blood ; metabolism ; Phenols ; blood ; Rabbits

BACKGROUND: Primary progressive aphasia(PPA) is a degenerative disease of nervous system, which is very rare in clinics. Only 3 cases have been reported in our country. There is very little clinical information regarding the characteristics of PPA in linguistics and imageology.OBJECTIVE: To report the language disorder and the characteristics of imageology of one PPA patient for the clarification of the clinical features of PPA.DESIGN: A case analysis.SETTING: Department of Neurology, Beijing Tiantan Hospital; Faculty of Psychology, Beijing Normal University.PATICIPANT: Male, 56 years old, senior high school graduate, businessman before the onset of the disease. The case visited the department of Neurology,Tiantan Hospital, Beijing due to the complaint of three years of progressive decreasing in language skills, which was then diagnosed with PPA.METH ODS: Spoken language fluency evaluation in Chinese Aphasia Examination Set of the First Affiliated Hospital of Beijing Medical University was used to evaluate this patient, the type of Aphasia was judged by Western Aphasia Examination Set, and the severity gradation was classified by the Boston Diagnostic Aphasia Examination(BDAE) . Memory, intelligence screening and imageology examination were performed as well.MAIN OUTCOME MEASURES: The fluency of spoken language, the type of aphasia, and the severity of aphasia of the patient were judged, and whether the patient suffered from memory and intelligence disorder were observed as well as the features of imageology.RESULTS: The patient had fluent spoken language, which was evaluated as sensory aphasia(SA) with the aphasia severity of level 4, and had no memory or intelligence disorder. MRI showed atrophy in left frontal lobe and temporal lobe, which was more significant in left side. MRI also showed that left temporal and frontal lobes had low metabolism and low perfusion.CONCLUSION: PPA is a disease with language disorder as its dominant clinical manifestation, which no other cognitive disorder at its initial stage,and with its main pathological changes in left temporal and frontal lobes.

Objective To study the temporal changes of alveolar epithelial type Ⅱ cells and surfactant pro-tein A in young rats with acute lung injury induced by lipopolysaecharide. Method Totally 110 SD young rats (male:53, female : 57) were randomly divided into ALI and normal control groups (six subgroups in each group).LPS(4 mg/kg) was given intraperitoneally in ALI group. The same amount of normal saline was given in the con-trol groups. Eight rats in each subgroup were sacrificed at 6, 12, 24, 36, 48 and 72 hours after the injection.Lung samples were taken for transmission electron microscope examination. RT-PCR was epmloyed for the mea-surement of SP-A mRNA. Western blot was used for the detection of SP-A in the lung tissue. ANOVA and homo-geneity of variance test were performed by SPSS 12.0. Results The microvilli disappeared at 24 hours after the injection of LPS. The number of lamellar body (LBs) was provisionality increased at 24 hours and 48 hours. The ring-like an'angement of LBs around nucleus and the giant LB with vacuole-like deformity were found as the main characteristics of AEC- Ⅱ in ALI at 48 hours. The number of LBs reduced and broken and residual LB remained at 72 hours. SP-A elevated greatly from 24 to 48 hours (P < 0.01), reached peak at 36 hours (6.94 ± 0.80, P <0.01),reached the lowest level(3.87 ±0.50, P <0.01)at 72 hours. Conclusions The pathological changes of AEC-Ⅱ and SP-A in lung tissue wiht ALI are time-dependent. The typical alterations of AEC- Ⅱ occurs at 48 hours accompanied by the compensatory increase of SP-A. AEC- Ⅱ is seriously injuried with the typical changes of LBs and the diminishing of SP-A in lung tissue.

Adult ; Age Factors ; Aged ; Aged, 80 and over ; Blood Viscosity ; China ; Female ; Humans ; Male ; Middle Aged ; Reference Values ; Sex Factors

Objective To evaluate the diagnostic efficiency of double-balloon enteroscopy in small bowel Crohn’s disease. Methods In sixty five patients with suspected small bowel Crohn's disease double-balloon enteroscopy were performed, and some of them received enteroscopy and enteroclysis, capsule endoscopy as well.Results The first enteroscopy was performed via mouth in 20 of 65 cases, and the lesions were detected in 11 cases (55%), 5 of 9 cases(55.6%) had lesions detected in enteroscopy via anus while nothing was found in mouth route. Among 45 cases examed by enteroscopy firstly via anus, 34 cases had lesions detected (75.6%), 8 of 11 cases(72.7%) had lesions found in following exam via mouth. Totally 58of 65 had lesions detected through enterosocpy examination, the overall diagnostic yield was 89.2%. Twenty four of 46 cases had positive findings with enteroclysis. The diagnosis of Crohn's disease was comfirmed in 14 of 22 patients(63.6%) underwent capsule endoscoy. The diagnosis was finally confirmed by enteroscopy only in 11 patients(78.6%).Conclusion The entire small intestine could be examined by enteroscopy with combination of mouth and anus route. Double-balloon enteroscopy was an ideal diagnostic modality for small bowel Crohn's diseases, which was also valuable in assessment on extent and severity of the disease. Small bowel enteroclysis was a useful screening alternative for selecting procedure route in DBE.