Birth Weight ; Female ; Gastrointestinal Diseases ; etiology ; therapy ; Gestational Age ; Humans ; Infant, Newborn ; Male

Animals ; Animals, Newborn ; Female ; Hyperbaric Oxygenation ; Hypoxia-Ischemia, Brain ; physiopathology ; therapy ; Nerve Growth Factor ; pharmacology ; Nerve Regeneration ; drug effects ; physiology ; Rats ; Rats, Wistar

ObjectiveTo investigate the application of surface electromyography (sEMG) in patients with lumbar disc herniation. Methods44 patients with chronic lumbar disc herniation were divided into mild pain group (group A) and moderate to severe pain group (group B) according to their scores of Visual Analogue Scale (VAS). Their sEMG at erector spinae and gastrocnemius were collected, and the average EMG (AEMG), slope of median frequency (MFs) were analyzed. ResultsCompared with those in the healthy side, AEMG of erector spinae and gastrocnemius reduced significantly (P<0.05) in affected side in both groups, while the MFs (absolute value) increased (P<0.05). Compared with those in group A, the MFs (absolute value) in affected side significantly increased in group B (P<0.05). The ratio of healthy/affected side of AEMG increased in group B (P<0.05), but the ratio of MFs was no significantly difference between 2 groups (P>0.05). ConclusionSurface EMG, of both the erector spinae and gastrocnemius, can be used as a non-invasive tool in detecting neuromuscular function of lumbar and lower limb in patients with lumbar disc herniation. The sEMG imbalance may respond with the severity of pain.

Objective To explore the quadriceps muscles function in patients with unilateral knee injury.Methods 23 unilateral knee injured patients with joint dysfuntion were assessed with isometric EMG activity of vastus lateralis (VL), vastus medialis obliques (VMO) and rectus femoris(RF) of both thighs during three 5-s maximal isometric voluntary contractions at a knee joint angle of 30° (0° - full knee extension), and the torque were recorded at same time. Results The average amplitude (AEMG), mean power frequency (MPF), and median frequency (MF) of VL, VMO and RF in affected knees were significantly lower than those in unaffected knees（P＜0.01）, as well as the torque（P＜0.01）. In the unaffected side, the AEMG, MPF of VL were higher than VMO and RF（P＜0.05）, and RF than VMO（P＜0.05）. In the affected side, the AEMG, MPF of RF are higher than VMO and VL（P＜0.05）, and VL than VMO（P＜0.05）. Conclusion sEMG can provide quantitative measurements of muscle function. The isometric strength and neuromuscular activation lever are lower in affected limb, RF may be affected less than VL and VMO in knee injured patients with knee dysfunction.

Objective To detect levels of serum platelet activating factor(PAF),thrombomodulin(TM) and white blood cell(WBC),platelet count(PLT) in neonates with meconium aspiration syndrome(MAS),and observe changes of mediators of inflammation and function of endotheliocute.Methods All cases were taken vein blood in 24 h and 72-96 h after birth.Surm PAF and TM were detected by EILSA technique,at the same time,blood cell counts were determined.Results PAF and WBC in neonates with MAS increased,which were relevant to the patients′ condition.TM of neonates with MAS increased significantly,especially in 72-96 h after birth and(aggrava)-ted with the patients′ condition.Conclusion Neonates with MAS have inflammatory reaction and injured endotheliocyte,which are(inte)-raction.

Objective To study the relationship of urine RBC morphology between UF-100 urine sediment analytic instrument andphase contrast microscope.Methods The UF-100 urine sediment analytic instrument to analyze 500 urine specimens and study the relation-ship of urine RBC morphology between urine sediment analytic instrument and phase contrast microscope.Results The according perceptionof Normocytic,Microcytic and Non-classified RBC between phase contrast microscope and UF-100 urine sediment analytic instrument RBC-info are 91.4%,94.4%,83.3% respectively,the according perception between phase contrast microscope and RBC-P70Fsc are 94.9%,95.7%,94.7% respectively,and the according perception between phase contrast microscope and RBC Fsc-DW are 84.4%,86.8%,90.5% respectively,the specificity of UF-100 and phase contrast microscope in glomerular hematuria and non-glomerular hematuria are84.3%,88.1% and 83.3%,87.9% respectively.Conclusion The results show that the UF-100 urine sediment analytic instrument issimply operating,fast and high accurate,and which can instruct clinical dignose,therapy and prognosis judgement.

Acute Kidney Injury ; epidemiology ; pathology ; Adolescent ; Adult ; Aged ; Biopsy, Needle ; methods ; China ; epidemiology ; Female ; Glomerulonephritis, Membranoproliferative ; epidemiology ; pathology ; Humans ; Kidney ; pathology ; Kidney Diseases ; epidemiology ; pathology ; Male ; Middle Aged ; Nephrotic Syndrome ; epidemiology ; pathology ; Ultrasonography, Interventional

Objective: To observe the clinical efficacy of moxibustion therapy plus Liu's pediatric massage (tuina) for children with recurrent respiratory tract infections due to qi deficiency of spleen and lung. Methods: A total of 60 children who met the inclusion criteria were divided into an observation group and a control group according to the visiting sequence, with 30 cases in each group. Children in the observation group were treated with moxibustion therapy plus Liu's pediatric massage, and those in the control group were treated with Liu's pediatric massage alone. The incidence of respiratory tract infections and traditional Chinese medicine (TCM) symptoms score were observed and recorded in both groups before and after treatment. And the clinical efficacy was compared between the two groups. Results: The total effective rate of the observation group was 93.3％, and that of the control group was 83.3％. The difference between the two groups was statistically significant (P<0.05). After treatment, the TCM symptoms score and total times of infections in both groups were all statistically different from those before treatment (all P<0.05). The differences in TCM symptoms score and infection frequency before and after treatment in the observation group were statistically different from those in the control group (both P<0.05). Conclusion: Moxibustion therapy plus Liu's pediatric massage has a better effect in improving the clinical symptoms and reducing the frequency of respiratory tract infections for children with recurrent respiratory tract infections due to qi deficiency of spleen and lung than the pediatric massage alone.

Adolescent ; Child ; Child, Preschool ; Chromosomes, Human, Pair 6 ; Female ; Humans ; Infant ; Loss of Heterozygosity ; Male ; Microsatellite Repeats ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; genetics

OBJECTIVETo isolate and identify nisin resistance determinant (NSR) gene from Lactococcus lactis.

METHODSThe Lactococcus lactis strains harboring NSR gene were isolated from different milk samples by selective culture supplemented with nisin and confirmed by PCR detection of 16S rRNA. Nisin resistance determinant gene was determined by PCR amplification, enzyme digestion and sequencing.

RESULTSThirty nisin-resistant Lactococcus lactis strains from fresh milk samples were obtained. Three of these strains contained NSR gene of about 1000 bp as determined by agarose gel electrophoresis and further confirmed by enzyme digestion and sequence analysis. The NSR gene was located on the plasmid of Lactococcus lactis.

CONCLUSIONComplete NSR gene, located on the bacterial plasmid, has been successfully isolated from nisin-resistant Lactococcus lactis strains from fresh milk.

Animals ; Anti-Bacterial Agents ; pharmacology ; Cattle ; Drug Resistance, Bacterial ; genetics ; Lactococcus lactis ; genetics ; isolation & purification ; Milk ; microbiology ; Nisin ; genetics ; pharmacology ; Plasmids ; genetics ; Polymerase Chain Reaction ; RNA, Ribosomal, 16S ; genetics