Objective To study the antibacterial constituents in the fermentation broth of marine actinomyces N331.Methods The antibacterial components were isolated and purified from the fermentation broth of strain N331 by absorption with macroporous resin and silica gel column chromatography,their physiochemical characteristics and bioactivities were preliminarily investigated.Results A crystal ingredient with bioactivities was isolated from the fermentation broth and it was identified as alkaloids which was composed of several compounds with approximate polarity,and its the minimum inhibitory concentration against sensitive and drug-resistant Staphylococcus aureus was 4?g?mL-1,and its half maximal inhibitory concentration(IC50) was 2.0,3.0?g?mL-1 respectively against KB and lung cancer A549 cells,which was equivalent to cisplatin.Conclusion Marine actinomyces N331 can produce the alkaloidal substances with strong antibacterial activity and cytotoxicity.

Objective: To observe the therapeutic efficacy of tuina plus cupping on chronic fatigue syndrome (CFS). Methods: A total of 100 CFS patients meeting the inclusion criteria were randomly divided into a treatment group and a control group according to the random number table in the sequence of visit, with 50 patients in each group. The treatment group was treated with tuina plus cupping therapy, 3 times a week. The control group was given oral tablets of wild American ginseng lozenges. After 3 months of treatment, the score of fatigue questionnaire scale (FS-14) was observed and the efficacy was evaluated. Results: The total effective rate was 92.0％ in the treatment group, versus 76.0％ in the control group and the difference between the two groups was statistically significant (P<0.05). After treatment, the FS-14 scores of the two groups decreased, and the intra-group differences were statistically significant (both P<0.05). The change of FS-14 score after treatment in the treatment group was more significant than that in the control group (P<0.05). Conclusion: Tuina plus cupping is effective for CFS, and can improve fatigue symptoms significantly.

Cells, Cultured ; Curcumin ; pharmacology ; Endothelium, Vascular ; drug effects ; pathology ; radiation effects ; Human Umbilical Vein Endothelial Cells ; drug effects ; radiation effects ; Humans ; Radiation Injuries ; Signal Transduction ; drug effects ; radiation effects

Bicuspid ; Dental Pulp Cavity ; pathology ; Humans ; Mandible ; Root Canal Therapy

Objective To understand the basic situation and the influencing factors of medical expenses of the inpatients in Haikou city for the government to developing a scientific control policy of medical expense or improving the existing measures for controlling the medical expense.Methods Totally 463 inpatients from 10 hospitals were conducted the questionnaire survey and the survey data were analyzed.Results The difference of medical costs constituent existed in different medical institutions and the me-dian of drug costs to the total medical costs in general hospital was 42.13%.30.02% (139/463)of the surveyed inpatients thought that the use of “new techniques,new drugs and new equipments”was the primary cause of medical costs rising.51.19%(237/463) of patients thought that the primary considered factor is “cure”when seeing a doctor.24.84%(115/463)of patients did not known the modification of medical insurance policy.46.00%(213/463)and 28.51%(132/463)of patients thought that the main responsi-bility main body for controlling the medical costs rising is government and hospital respectively.Conclusion Increasing the medical insurance policy advocacy,promoting the reform of medical insurance payment methods,strengthening the dominant position of gov-ernment control medical costs and rationally allocating the medical resources conduce to reduce the medical costs rising.

10.3969/j.issn.2095-4344.2013.23.007

AIM: To study the determination of dl-tetrahydropalmatine (THP) in Chi nese traditional patent medicines by TLC-Scanning from the viewpoint of the pro cess control of manufacture. METHODS: To select the 5 kinds of representative drugs and its prep arations contained, THP was extracted with solvent method. The mobile phase contains diethylamine. THP w as separated with high-efficiency silica gel plate or handcrafted silica gel p late. Iodine was used for chronogenic agent and fluorescence defectiion for dete rmination. TLC-Scanning results were compared with HPLC. RESULTS: The method is practicable, the result is correlative with the HPLC. CONCLUSION: TLC-Scanning is fit for the quality control of the Chi nese traditional patent medicine in the process of manufacture.

Objective:To study the epidemiology of attention-deficit hyperactivity disorder(ADHD) in pupils of Zhenjiang urban districts. Methods: Questionnaire was designed and employed for parents of 3 698 pupils at schools in Zhenjiang urban districts. ADHD was diagnosed according to the international DSM-Ⅳ scale. Differences were determined by Qi-square test and multiple regression analysis. Results: Prevalence of ADHD was 6.54% in pupils of Zhenjiang urban districts, and was 9.14% in male pupils and 4.01% in female. There was significant difference between 2 gender groups (? 2=34.933,P

Objective To systematically evaluate the effectiveness of diabetes self-management education through telemedicine on glycosylated hemoglobin (HbA1c). Methods Randomized clinical trials (RCTs) that assessed the effectiveness of remote diabetes self-management education in patients with diabetes were systematically reviewed using multiple electronic databases. The searching time was from database establishment to May 2015. These articles were analyzed after evaluation of their quality. Results Eight RCTs were included involving 2 047 patients.The results of Meta-analysis showed the effectiveness of remote DMSE on reduction of HbA1c of patients with type 2 diabetes was more significant than that of the control group [WMD=-0.50,95%CI (-0.88,-0.12),P < 0.05],while failing to support the effectiveness of remote DMSE on patients with type 1 diabetes [WMD=0.62, 95%CI(-0.19,1.43), P>0.05]. Conclusions Remote DMSE is effective to improve the glycemic control in patients with type 2 diabetes,while the effect on patients with type 1 diabetes is still not certain. Large-scale and multi-centered RCTs are needed to confirm its effectiveness.

Objective To resolve the difficulty in controlling the transfusion speed by medical personnel.Methods By using liquid drop sampling module,pulsing signals of liquid drop were obtained,and then they were enlarged,shaped and the wrong signals from the top and bottom surface of every drop were excluded before the standard triggered signals were sent out to SCM processing circuit,from which the real-time liquid drop speed and the average drop speed in every minute were obtained.Results Anti-interference and portable transfusion-drop counter could show the real-time liquid drop value and the average liquid drop value in every minute.Conclusion Anti-interference and portable transfusion-drop counter is convenient with simple structure and has been used in clinic.