Aiming at the confused situation of Stephania tetrandra and its adulterants in the production,supply and sale market,the author had overviewed information on Stephania tetrandra and its adulterants in source,character identification,microscopic identification,physical and chemical identification at home and abroad in recent years,so thus for scientific identify of species,distinguishing between the families and genera and correcting medication for clinical use.

Objective To evaluate the effects of dexmedetomidine on inflammatory responses in lung tissues during one-lung ventilation (OLV) in patients undergoing esophageal cancer resection.Methods Forty American Society of Anesthesiologists physical status Ⅰ or Ⅱ patients,aged 25-65 yr,with body mass index of 18-25 kg/m2,scheduled for elective radical resection of esophageal cancer,were divided into 2 groups (n=20 each) using a random number table:dexmedetomidine group (group D) and control group (group C).Dexmedetomidine was infused intravenously in a loading dose of 0.6 μg/kg over 10 min before induction of anesthesia,followed by an infusion of 0.4 μg · kg-1 · h-1 until 30 min before the end of operation in group D,and the equal volume of normal saline was given instead in group C.After intubation (T1),at 30,60 and 90 min of OLV (T2-4),and at 30 min after restoration of two-lung ventilation (T5),blood samples were taken from the internal jugular vein.Broncho-alveolar lavage fluid (BALF)in ventilated lung was collected at T1-4.The concentrations of tumor necrosis factor-alpha (TNF-α),interleukin-6 (IL) and IL-10 in plasma and BALF were determined by enzyme-linked immunosorbent assay.Results Compared with group C,the plasma concentrations of TNF-α at T5 and IL-6 at T4,5 were significantly decreased,the plasma concentration of IL-10 was increased at T4,5,and the concentration of TNF-α in BALF at T4 and concentration of IL-6 in BALF at T3,4 were decreased,and the concentration of IL-10 in BALF was increased at T4 in group D (P＜0.05).Conclusion Dexmedetomidine can significantly ameliorate the inflammatory responses in lung tissues during OLV,thus reducing systemic inflammatory responses in patients undergoing esophageal cancer resection.

Objective To test the in vitro effect of the extract of Herba taraxaci on Demodex folliculorum. Methods Active Demodex folliculorum were obtained from patients with moderate to severe demodex infestation. Herba taraxaci and Radix stemonae were extracted respectively with 80% ethanol under 85℃, and a preparation with a concentration of 200mg/ml was made. The extractions were used in vitro to examine the anti-mite activity by observing time of killing mites. Physiological saline and Radix stemonae extraction served as blank control and positive control respectively. PH value of Herba Taraxaci extract was noted. Skin irritation test of normal and wounded skin and acute toxicity test were carried out with rabbit shin, and Herba taraxaci and 75% ethanol were served as experiment and control medicine. Results Motion and morphology of the mites considerably changed with the effect of Herba taraxaci extract. The time of mite-killing was (1.50?0.65)min with Herba taraxaci and (3.53?1.04)min with Radix stemonae respectively (P

The severity of ischemic brain injury was closely associated with the mortality and disability of stroke. How to limit ischemic brain injm-y to the greatest extent and promote the recovery of neurological function is one of the most concerned hotspots at present in the field of neuroscience. A large number of studies have shown that a variety of factors involve in the pathological process of ischemic brain injury, in which, the role of T lymphocyte-mediated immune inflammatory response in ischemic brain injury has received increasing attention. This article mainly reviews the role of T cells in ischemic brain injury.

Objective To evaluate the efficacy of pressure-controlled volume-guaranteed (PCVVG) mode for lung protective ventilation in patients requiring one-lung ventilation (OLV) during thoracoscopic surgery.Methods Sixty patients,aged 50-70 yr,with body mass index of 18-26 kg/m2,of American Society of Anesthesiologists physical status Ⅰ or Ⅱ,scheduled for elective radical resection of esophageal cancer performed via video-assisted thoracoscope under general anesthesia,were divided into 2 groups (n=30 each) using a random number table:volume-controlled ventilation group (group V) and PCV-VG group (group P).The ventilator settings were adjusted,with a tidal volume 10 ml/kg and respiratory rate 10-12 breaths/min during two-lung ventilation,and with a tidal volume 6 ml/kg and respiratory rate 12-16 breaths/min during OLV.The inspiratory/expiratory ratio was 1 ∶ 2,pressure restriction was 35 cmH2O,and 33％ oxygen was inhaled at 2 L/min.The end-tidal pressure of carbon dioxide was maintained at 35-40 mmHg.Visual analog scale score was maintained ≤ 3 after operation.After admission to the operation room (T0) and at 1,3 and 7 days after operation (T1-3),forced vital capacity (FVC),forced expiratory volume in first second (FEV1),and maximal mid-expiratory flow (MMEF) were measured,arterial blood samples were collected for blood gas analysis,arterial carbon dioxide partial pressure and arterial oxygen partial pressure (PaO2) were recorded,and alveolar-arterial oxygen tension difference (PA-a O2) was calculated.Clinical Pulmonary Infection Score was assessed at T1,T2 and T3.The chest tube removal time and length of postoperative hospital stay were recorded.Results Compared with the baseline at T0,FVC,FEV1,MMEF and PaO2 were significantly decreased,and PA-aO2 was increased at T1-3 in the two groups (P＜0.05).Compared with group V,FVC,FEV1,MMEF and PaO2 were significantly increased,PA-aO2 and Clinical Pulmonary Infection Score were decreased,and the chest tube removal time and length of postoperative hospital stay were shortened at T1-3 in group P (P＜0.05).Conclusion PCV-VG mode can achieve lung protective ventilation,which is helpful in improving outcomes in the patients requiring OLV during thoracoscopic surgery.

Objective To investigate the therapeutic effects and the possible mechanism of fecal transplantation on rats with Clostridium difficile-associated pseudomembranous colitis. Methods A total of 40 Sprague-Dawley rats were divided into four groups including the healthy control group, model group, fecal transplant treatment group and vancomycin treatment group. Rats in three experimental groups were subcuta-neously injected with clindamycin phosphate (10 mg), followed by treatment with toxin producing Clostridi-um difficile (ACTT43255) enema 24 hours later. The rats in fecal transplant treatment group and vancomy-cin treatment group were respectively treated with fecal suspension and vancomycin one day after modeling. The rats were fasted for one day after the last administration and then executed. The levels of potassium ion ( K) , sodium ion ( Na) , albumin ( ALB) , white blood cells ( WBC) , C-reaction protein ( CRP) , interleu-kin-1β ( IL-1β) , interleukin-10 ( IL-10 ) , interleukin-12 ( IL-12 ) and interleukin-17 ( IL-17 ) as well as the percentage of neutrophils ( N%) in serum samples were detected. The colon tissue samples were collect-ed for pathology examination. Results The rat model of pseudomembranous colitis was successfully estab-lished by subcutaneous injection of clindamycin in combination with toxin-producing Clostridium difficile (ACTT43255) enema. The signs of intestinal inflammation including serious weight loss, remarkably short-ened colon length and significantly increased colon wet weight index were observed in rats from the model group (P<0. 05). Compared with the rats from model group, the rats received fecal transplant showed sig-nificantly increased levels of K, ALB, IL-10 and IL-10/IL-12 in serum and decreased levels of WBC, N%, CRP, IL-1β and IL-17 (P<0. 05). Conclusion Fecal transplantation was proved to be an effective ap-proach for the treatment of pseudomembranous colitis. The therapeutic mechanism might due to its impacts on serum inflammatory factors.

Objective To examine the expressions of AQP1 and VEGF in human hepatitis B associated hepatocellular c arcinoma and to investigate its relationship with the occurrence and development of hepatocellularcarcinoma. Methods AQP1 and VEGF expressionsin the cancer tissues and AQP1 and VEGF levels in the serum were detected in 62 cases of hepatocellula carcinomaby immunohistochemistry and ELISA , respectively. Results The expressions of AQP1 and VEGF in the liver cancers were significantly higher than in the normal tissues (P<0.01). The higher expression was related to pathologic type,degrees of differentiation and lymph node metastasis, but not related to sex, age and TNM stages. In the serum of liver cancer, the concentrations of AQP1 and VEGF were significantly higher than those in the control group. Conclusion High expressions of AQP1 and VEGF in the patients with liver cancer may be correlated to the occurrence and development of liver cancer. Plasma levels ofAQP1 and VEGF may be more meaningful for the evaluation of clinical prognosis.

Objective To develop an equipment purchase process management module based on B/S architecture to standardize hospital equipment purchase process and promote hospital informatization. Methods Service-oriented architecture (SOA) was adopted as the main architecture, and internet information service (IIS) manager was used as the publishing tool.Results All the departments could visit the system through hospital intranet and perform on-line equipment purchase and inquiry for progress.Conclusion The system facilitates the inquiry of all departments for the progress of equipment purchase, and standardizes and optimizes the equipment purchase process.

Objective To investigate the relationship between vascular endothelial function and insulin resistance(IR) and the incidence of metabolic syndrome(MS).Methods A seven-year follow-up study was conducted in 583 simple obese subjects who were chosen in 2000.Body fat,blood pressure,lipid profile,plasma glucose,insulin resistance index(HOMA-IR) and endothelium- dependent dilatation(EDD) were measured before and after the follow-up.Results A total of 177 new cases with MS were found during this seven years,which was 33.78% of all subjects who were followed up in 2007.Cumulative incidence of MS among seven-year was 38.14% in the impaired EDD group and 48.11% in the impaired EDD with IR group,both rates were higher than that of the IR group(25.00%)(P

A bacterium that contains a chitin deacetylase has been isolated from ocean soil. It has been identified Baciluus .The best suitable condition of producing this enzyme is that pH is 4.0,35℃,and the incubate time is 80 hours in the present of Ca 2+ as well as having no chitosan. The best temperature of this enzyme when it acts is 40℃～50℃ and the best pH range is 4.5～5.0.