1.Associations of body mass index with metabolic status and chronic complications in newly-diagnosed type 2 diabetic patients
Hong-Yan WU ; Lu-Lu CHEN ; Juan ZHENG ; Yun-Fei LIAO ; Min ZHOU ;
Chinese Journal of Endocrinology and Metabolism 1986;0(04):-
Objective To investigate the association of body mass index(BMI)with metabolic status and chronic complications in newly-diagnosed Chinese type 2 diabetic patients.Methods A total of 515 newly- diagnosed adults type 2 diabetic patients were categorized into underweight(BMI
2.Genetic polymorphism of nine non-CODIS STR loci in Hunan Province-based Chinese Han population.
Juan-juan GUO ; Ying LIU ; Ya-dong GUO ; Jie YAN ; Yun-feng CHANG ; Ji-feng CAI ; Ting LU ; Zha LAGABAIYILA
Journal of Forensic Medicine 2014;30(6):441-445
OBJECTIVE:
To determine the allelic frequency distribution and genetic parameters of nine non-CODIS DNA index systems of the short tandem repeat (STR) loci (D2S1772, D6S1043, D7S3048, D8S1132, D11S2368, D12S391, D13S325, D18S1364, and GATA198B05).
METHODS:
A total of 353 blood samples were collected, extracted, amplified, and analyzed from unrelated healthy individuals of Han nationality in Hunan Province, China.
RESULTS:
One hundred and fourteen alleles were observed in the population with corresponding allelic frequencies ranged from 0.001 0 to 0.323 0. For all the nine non-CODIS STR loci, the observed genotypic data showed no significant deviations from the Hardy-Weinberg equilibrium. The Ho, He, PIC, DP, and PE of the studied non-CODIS STR loci ranged from 0.1080 to 0.1950, 0.8050 to 0.8920, 0.7700 to 0.8600, 0.9250 to 0.9660 and 0.6070 to 0.7800, respectively.
CONCLUSION
Nine non-CODIS STR loci have high degrees of polymorphisms, which may be useful in individual forensic identification and parentage testing in forensic practice.
Alleles
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Asian People/genetics*
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China
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Ethnicity/genetics*
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Gene Frequency
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Genetics, Population
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Genotype
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Humans
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Male
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Microsatellite Repeats/genetics*
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Polymorphism, Genetic
3.Reversal of drug resistance in human ovarian cancer cells by wild-type PTEN gene and its mechanisms
Hui-Juan WU ; Hai-Tao WU ; Dan-Hui WENG ; Hui XING ; Yun-Ping LU ; Ding MA ;
Chinese Journal of Obstetrics and Gynecology 2000;0(09):-
Objective To examine expression of PTEN gene in ovarian cancer cisplatin-sensitive cell line OV2008 cells and cisplatin-resistant cell line C13K cells,and evaluate the effect of wild-type PTEN gene on reversing cisplatin-resistance of C13K cells and underlying mechanisms.Methods The expression of PTEN mRNA and protein in OV2008 and C13K cells were detected by semi-quantitative RT-PCR and western blot.Recombinant eukaryotic expression plasmid containing human wild-type PTEN gene was transfected into C13K cells by lipofectamine 2000.The expression of PTEN mRNA was monitored by RT- PCR and the expression of PTEN,protein kinase B(AKT),phospho-AKT(p-AKT)protein were analyzed by western blot in PTEN transfected and untransfected C13K cells.Proliferation and chemosensitivity of cells to cisplatin were measured by methyl thiazolyl tetrazolium(MTT),and cell apoptosis was detected by flow cytometry after treatment with cisplatin.Results(1)The expression of PTEN mRNA and protein(1.02 ?0.05,1.02?0.07)in OV2008 cells were significantly higher than those in C13K cells,which were 0.45 ?0.03 and 0.55?0.03 respectively(P
4.Application of early cleavage embryo in combination with embryo growth rate and morphology scoring in embryo selection
Yi-juan, SUN ; Ai-jun, ZHANG ; Xiao-wei, LU ; Zhi-hong, NIU ; Qian, CHEN ; Yun, FENG
Journal of Shanghai Jiaotong University(Medical Science) 2009;29(6):719-721
Objective To evaluate the role of early cleavage embryo in combination with embryo growth rate and morphology scoring in embryo selection in in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) cycles. Methods Six hundred and ten IVF/ICSI cycles were randomly assigned to group A(269 cycles) and group B(341 cycles). In group A, transferred embryos were chosen according to embryo growth rate and morphology scoring by 72 h(D3) after fertilization, while early cleavage embryo was added to the selecting system in group B. The pregnancy rate and implantation rate were compared between two groups, and the clinic outcomes were compared between transfers with early cleavage embryos and without early cleavage embryos in group B. Results The pregnancy rate and implantation rate in group B were significantly higher than those in group A (P < 0.05). Transfers with early cleavage embryos also achieved much higher pregnancy rate and implantation rate in group B (P < 0.01). Conclusion Compared with embryo growth rate and morphology scoring, early cleavage embryo in combination with embryo growth rate and morphology scoring can improve the clinical outcomes in IVF/ICSI cycles.
5.Induction of experimental Graves' disease in Balb/c mice immunized with human thyrotropin receptor ectodomain amino terminus gene
Yun-juan, ZHU ; Zi-qin, ZHAO ; Lan-ying, LI ; Feng-xian, LU ; Zhi, YAO
Chinese Journal of Endemiology 2008;27(3):242-246
Objective To study the antigenicity of human thyrotropin receptor(hTSHR)amino terminus (amino acid 29~280)and its association with Graves' disease.Methods Total thyroid RNA was prepared from human normal thyroid tissue.RNA was then reversely transcripted and cDNA was subjected to PCR amplification.PCR product was cloned into pcDNA3.1 and the recombinant plasmid was named pcDNA3.1/hTSHR188~940bp. Balb/c mice were immunized with peDNA3.1/hTSHR188~940bp. The levels of serum thyroxin,anti-TSHR antibody(TRAb)and thyroid stimulating antibody(TSAb)were measured,and the pathological changes of thyroid tissue were also observed.Results A 753 bp fragment encoding hTSHR ectodomain amino end was obtained after PCR amplification.Confirmed by Hind Ⅲ restriction enzyme digestion and DNA sequencing,pcDNA3.1/hTSHR188~940bphad been constructed successfully,with the correct sequence and direction of hTSHR188~940bp.In the Balb/c mice treated with pcDNA3.1/hTSHR188~940bp,elevated TRAb in week 6(0.148±0.018)were observed compared with those at week o(0.106±0.006,P<0.01),and kept a higher level till week 10(0.134±0.011,P<0.01).T4 and TSAb index values were significantly increased in week 10.Serum T4 concentration increased from(41.02±7.97)μg/L in week 0 to(62.20±12.77)μg/L in week 10(P<0.01);TSAb index values rose from 0.864±0.076 at week 0 to 1.392±0.615(P<0.01).Thyroid pathological examination showed that proliferated thyroid follicular epithelial cells and foll icular eapacity increased.Inflammatory cells were occasionally found.Conclusions There are antigen epitopes in hTSHR ectodomain amino acid 29~280,which can stimulate the production of TSAb.And the latter induces hyperthyroidism and Graves' disease like manifestations.It suggests that hTSHR ectodomain amino acid 29~280 is closely associated with Graves' disease,and maybe one of important etiological factors leading to the disease.
6.Villoglandular adenocarcinoma of cervix:a clinicopathological study.
Zheng-cao LIU ; Lu ZHENG ; Yun-long HUO ; Xiang-hong YANG ; Ai-feng GAO ; Xiu-juan CUI
Chinese Journal of Pathology 2010;39(5):338-339
Adenocarcinoma
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metabolism
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pathology
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surgery
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Adenocarcinoma, Clear Cell
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metabolism
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pathology
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Adult
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CA-125 Antigen
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metabolism
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Carcinoembryonic Antigen
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metabolism
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Cervical Intraepithelial Neoplasia
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metabolism
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pathology
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surgery
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Cystadenocarcinoma, Serous
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metabolism
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pathology
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Diagnosis, Differential
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Female
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Follow-Up Studies
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Humans
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Hysterectomy
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Ki-67 Antigen
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metabolism
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Lymph Node Excision
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Membrane Proteins
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metabolism
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Neoplasm Invasiveness
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Uterine Cervical Neoplasms
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metabolism
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pathology
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surgery
7.Interlaboratory method validation of HPLC-FMA for determination of polysorbate 80 in monoclonal antibodies
Xiao-juan YU ; Chuan-fei YU ; Rong-jian ZHANG ; Gang WU ; Yong-fei CUI ; Lu-yun GUO ; Lan WANG
Acta Pharmaceutica Sinica 2021;56(8):2276-2281
The high performance liquid chromatography-fluorescence micelle assay (HPLC-FMA) method for the content determination of polysorbate 80 in monoclonal antibody drugs was validated to study its applicability and transferability between various laboratories, and the feasibility to be included in the Chinese Pharmacopoeia. Both J.T. Baker and Nanjing Well-sourced polysorbate 80 was used in the collaborative validation of polysorbate 80 content analysis in seven different laboratories. The results show that when the protein concentration was no more than 20 mg·mL-1 and the concentration of polysorbate 80 ranged from 0.05 to 0.5 mg·mL-1, the method had good specificity. The recovery rates of the spiked samples ranged from 92.20% to 117.70% for J.T.Baker and from 93.90% to 117.20% for Nanjing Well. The intra-laboratory precision (%RSD) was less than 4.30% for J.T. Baker, and less than 2.60% for Nanjing Well, while the overall precision was less than 5.45% for J.T. Baker, and less than 6.70% for Nanjing Well. The linear correlation coefficient was more than 0.98 for J.T. Baker and more than 0.99 for Nanjing Well. The results of the collaborative validation prove that the HPLC-FMA method has good accuracy, precision, linearity, and specificity, and could be used for release control analysis of polysorbate 80 content in monoclonal antibodies across different laboratories.
8.Expression and Antigenic Analysis of the Recombinant Epitope of Herpes Simplex Virus Type 2 Glycoprotein G
Xiao-Hong WANG ; Hai-Rong LU ; Gang ZHANG ; Shao-Juan CHEN ; De-Xin HUANG ; Ling-Yun LI ; Feng LIN ;
China Biotechnology 2006;0(09):-
A fragment containing amino acid residues 561~578 of HSV-2 glycoprotein G(gG2) was obtained by PCR assembling technique,and doubly cloned into vector pET-KDO.The recombinant plasmid was transformed to BL21(DE3)plysS.Fusion protein,of molecular weight about 39kDa was highly expressed by induction of IPTG.Western blot result showed the fusion protein had good antigenicity.After putification and digestion,the purity reached 95%.The digested purified protein was analysed by ELISA and showed good sensitivity and specificity.The recombinant protein should be useful for type-specific serodiagnosis of HSV-2.
9.Molecular cytogenetic detection of partial chromosome 13q trisomy and its relation with the clinical features of tortilcollis.
Juan DU ; Yue-qiu TAN ; Lu-yun LI ; Guang-xiu LU
Chinese Journal of Medical Genetics 2003;20(3):189-192
OBJECTIVETo search for the possible relation between tortilcollis and partial chromosome 13q trisomy.
METHODSFluorescence in situ hybridization (FISH) technique combined with chromosome banding was performed to determine the karyotype of two patients with typical clinical features of partial 13q trisomy syndrome, then their manifestations were compared with those of the literatures published previously.
RESULTSThe two cases were partial trisomy of 13q14--> ter with a different second derivative chromosome, in spite of this difference, both of them had tortilcollis.
CONCLUSIONIt is suggested that a potential site for tortilcollis may locate on the long arm of chromosome 13. With reference to a report previously published, the more precise candidate related region may be 13q32--> qter.
Child ; Chromosome Banding ; Chromosome Deletion ; Chromosomes, Human, Pair 13 ; genetics ; Cytogenetics ; Humans ; In Situ Hybridization, Fluorescence ; Karyotyping ; Male ; Torticollis ; genetics ; Trisomy ; genetics
10. Analysis of the characteristics of lymphocyte subsets in peripheral blood among coal worker’s pneumoconiosis patients
Jun LU ; Jing LI ; Qingyun HAN ; Xueer LU ; Yun HU ; Huichun CHEN ; Juan HU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2018;36(5):353-356
Objective:
To preliminary analysis of the characteristics of lymphocyte subsets in peripheral blood among 135 cases of coal worker’s pneumoconiosis patients in Huainan mining area.
Methods:
The peripheral bloods of 135 cases of coal worker’s pneumoconiosis patients and 112 cases of health examiners were collected. Flow cytometry was used to detect peripheral blood lymphocytes, T cell subsets and CD4+CD25+ regulatory T cells.
Results:
Compared with the control group, CD64 index of granulocytes and lymphocytes was slightly higher. The total T cells (CD3+) increased in peripheral blood, CD4+ expression was reduced and CD8+ expression was increased in infection group, CD4+/CD8+ ratio was inverted, the differences between the infection group and the control group were statistically significant (