The effect on the growth and phenol degradation of the thermophilic strain BF80 by seventeen different metal ions were studied. The results showed that the metal ions certainly affected the growth and phenol degradation of the strain BF80. At the concentration of 0.01%,contrasting to the comparison,the growth and phenol degradation of the strain BF80 were restrained intensively by the metal ions of Cu2+,Zn2+,Co2+,Ba2+,Hg2+,Ni2+,Ag+,Al3+. The metal ions of Cr2+ restrained the phenol degradation of BF80 strongly while Cr2+ restrained the growth of BF80 faintly. The metal ions of Sn2+,Fe2+,Fe3+,Pn2+ restrained the growth and phenol degradation of the strain BF80 at a certain concentration,and the effect of inhibition in-creased with the increase of the concentrations of the metal ions. At the low concentration of Mn2+ or Mo2+,the growth and phenol degradation of the strain BF80 were increased,but if the concentration beyond the 0.1%,the growth and phenol degradation of the strain BF80 were inhibited. At the different concentration of Ca2+ or Mg2+,the growth of the strain BF80 were increased and the phenol degradation were accelerated,but the max rate of phenol degradation by BF80 was not influenced obviously. When the medium was added themixture of metal ions of Mo2+ and Mn2+,the strain BF80 grew better,but the rate of the phenol degradation was lower than that of single Mo2+ and Mn2+.

0.05).The required dose of LT4 during the second and third trimester was respectively,remarkably increased compared to pre-gestational period (P

Objective To study the clinical effect of SCOFIX on thoracolumbar fracture.Methods The clinical data of twenty-five cases with thoracolumbar fracture treated with SCOFIX system were retrospectively anal- ysed.Results With six to twenty-two months follow-up,anterior height of compressed vertebral bodies was restored from 46.8 %(12%～60%)preoperatively to 92%(90%～100%)postoperatively,and that of posterior height was from 77.5%(26%～86%)to 96.7%(90%～100%).Cobb's angle was from 15.7?(12?～26?)preoperatively to 1.4?(0?～6?)postoperatively.Conclusion SCOFIX system was simple structured,easily used and strongly fixed, and it was a good system in spinal internal fixation and for deeply reseach.

Objective To evaluate the effect of tissue factor (TF), tissue factor pathway inhibitor-1,2 (TFPI-1,2) on cancer metastases and thrombosis complicated with cancer. Methods Blood samples from 292 cancer patients were collected and were divided into different teams according to cancer types,complicated with or without thrombosis; TF, TFPI-1, TFPI-2, plasma concentrations were measured by ELISA;Tissue expression of TF, TFPI-1, TFPI-2 were observed by immunohistochemical method. Results Plasma concentrations of TF and TFPI-1 in all kinds of cancers were higher than the control and lung cancer was the highest; TFPI-2 plasma concentrations had no statistics differences among all these teams. Tissue expression of TF of all kinds of cancer were higher than the adjacent tissues, lung cancer was higher than the other types of cancer. There were no statistics differences for TFPI-1 and TFPI-2. Both TF and TFPI-1 plasma concentrations of cancer with-or without-thrombosis were higher than control. TF was even higher in cancer with thrombosis team, TFPI-1 had no statistic difference between these two teams. TFPI-2 concentrations had no differences among all these teams. Conclusion Many kinds of tumor have higher expression of TF, it is expressed with different intensity according to different types of cancer. TFPI-1 has no clear effect in cancer growing and metastases. Unbalance of TF and TFPI-1 in plasma may relate to high coagulation state of cancer and may accelerate the thrombosis formation in cancer.

Identification and validation of a new target is one of the most important steps for new antituberculosis (TB) drug discovery. Researches have shown that Mycobacterium tuberculosis (Mtb) encodes 20 CYP450 enzymes which play important roles in the synthesis and metabolism of lipid, cholesterol utilization, and the electron transport of respiratory chain in Mtb. With the critical roles within the organism as well as the protein structures of six Mtb CYP450 enzymes being clarified, some of them have been highlighted as potential anti-tuberculosis targets. In this paper, the phylogenetic analysis, the structural features, and the enzymatic functions of Mtb CYPs, as well as the mechanism of interactions with selective inhibitors such as azole antifungal agents for the CYPs have been reviewed and summarized. The druggability of the CYPs has also been analyzed for their further utility as targets in high throughput screening and rational design of more selective inhibitors.
Antitubercular Agents ; chemistry ; pharmacology ; Azoles ; chemistry ; pharmacology ; Cytochrome P-450 Enzyme Inhibitors ; chemistry ; pharmacology ; Cytochrome P-450 Enzyme System ; genetics ; metabolism ; Drug Delivery Systems ; methods ; Drug Discovery ; Humans ; Mycobacterium tuberculosis ; drug effects ; enzymology ; genetics ; Phylogeny ; Tuberculosis ; drug therapy ; microbiology

It is important and difficult to establish the market competition mechanism in the health care re-form. Medical voucher system in Hong Kong and Macao can provide policy guidelines for Mainland China to promote institutional innovation, force public hospital reform and the rational allocation of medical and health resources. This paper introduced the origin and development of medical voucher system. Based on the description of the implementa-tion background, similarities and differences and effects of medical voucher system in Hong Kong and Macao, the pa-per found that medical voucher system could help encourage the demander to make more frequent use of medical serv-ices, improve their consciousness of prevention and health care and promote family doctor system. Through analyzing the applicability of medical voucher system in mainland China, the paper pointed out it was consistent with the reform orientation and could be served as a useful supplement to the health care system to improve medical insurance, medi-cal assisstance system as well as an effective measure to develop private medical institutions.

AIM: To investigate the processing and HPLC fingerprint of Radix Scutellariae processed with wine,and to set up appropriate quanlity control standard. METHODS: chromatographic condition of HPLC-UV fingerprint consisted of Hypersil C_18 column(200 mm?5.0 mm,5 ?m),mixture of methanol,0.4% phosphoric acid and acetonitrile as a mobile phase in a gradient mode.Flow rate was 1.0 mL/min and detection wavelength was set at 277 nm. RESULTS: There were no evident differences among fingerprints of Radix Scutellariae that was normatively processed from the production areas. CONCLUSION: The process is feasible,and can be used to provide a basis for quanlity control of Radix Scutellariae.

AIM To evaluate the effect of METHODS 2 month-old mice were fed routinelycistanche desertica polysacchrides(CDP) on the ni- for 11 mounths to establish the model of naturallytric oxids(NO) ,superoxide dismutase(SOD) in se- aging mice. The cistanche desertica polysacchridesrum and apoptosis of lung cell in aging mice. 25, 50, 100 mg ?kg~' were administrated respec- lively to the mice po. then investigated the concentration of NO, SOD and the morphological changes of apoptosis by fluoromicroscope. RESULTS Aging could induce the lung cell apoptosis, decrease the level of SOD, and increase the level of NO as well. The cistanche desertica polysacchrides signif- icantly ameliorated the lung cell apoptosis; decreased the level of NO, improved the activity of SOD. CONCLUSION Cistanche desertica polysac-chrides have ameliorating and postponing effects on the regressive changes in the lung of aging mice.

Objective To optimize the extraction process f or polysaccharide in the residue of Fructus Jujubae(FJ ).Methods The conditions of extraction process for polysaccharide of FJ was screen ed with the yield and content of polysaccharides as markers by orthogonal experimental design.Results The optimum extraction process was:soaking the residue of FJ with water at 90℃for3times(5hours each time )and precipitated with 80%ethanol.T he yield and content of polysaccharides were 20%and 43.54%respectively.Conclusion This procedure is reliable and polysac-charides can be extracted completely by this procedure.It can bring a hig h economy benefit.

Objective: To establish the quality standard for Wuziyanzong Pills (Fructus Lycii, Semen Cuscutae, Fructus Rubi, Fructus Schisandrae, Semen Plantaginis). Methods: Betaine, the index component of Wuziyangzong Pills was determined by dual wavelength TLC-scaning, ? s=514nm, ? R=468nm. Semen Cuscutae and Fructus Schisandrae were identified by TLC. Results: The average recovery was 99.28% and RSD was 2.66%(n=5), respectively. Conclusion: The method is reliable, accurate and specific. It can be used for the quality control of Wuziyanzong Pills.