1.Determination of 2,3,5,4′-Tetrahydroxystilbene-?-O-?-D-Glucoside in Radix polygoni multiflori preparata by HPLC
Yun LIU ; Dong ZHANG ; Hua YUAN
Chinese Traditional Patent Medicine 1992;0(11):-
AIM: To establish HPLC for determination of 2,3,5,4′tetrahydroxystilbene-?-O-?-D-glucoside in Radix polygoni multiflori preparata. METHODS: The column was diamonsil C_(18)(5 ?m,4.6 mm?250 mm).The mobile phase was CH_3CN-CH_3OH-H_2O(10∶20∶70) and ?=320 nm.The flow rate was 1.5 ml/min. RESULTS: The linearity was in the range of 0.0253-0.3542 ?g.The average recovery and RSD were 99.34% and 0.49%,respectively. CONCLUSION: The method is simple,precise and reproduciable and can be used for determination of 2,3,5,4′-tetrahydroxystilbene-?-O-?-D-glucoside in Radix polygoni multiflori preparata effectively.
2.Modulatoin of Macrophage-Mediated Cytotoxic Activity Thibitory Effect of a Mycotoxin, Fusarin C, on Macrophage Activation in Mice
Zhong-Yun DONG ; You-Hui ZHANG ;
Chinese Journal of Immunology 1985;0(06):-
The effect of Fusarin C, a new mutagen extracted from Fusarium moniliforme,on murine peritoneal M? activation by measuring M?-mediated MTC ADCC and CS,Fusarin C could inhibit M? activation by both MAF and FDP. The inhibitory effect was dose-and time-dependent and the curves of dose-and time-response were similar in all three assays. There is, however, apparent difference in Fusarin C dose and time needed to induce significant in hibition among these assays. Significant inhibition occured at 0.4?g/ml and 0.5?g/ml for MTC and CS respectively, but 1.6?g/ml for ADCC. Similarly, the minimal period of time necessary to bring about significant inhibition was 2 hr for MTC, but 3 hr for ADCC. Finally, significance of the inhibitory effect of Fusarin C on M? anti-tumor activity in relation to carcinogenesis was discussed.
3.Modulation of macrophage-mediated cytotoxic activity Ⅱ.The Inhibitory effect of fusarin C on macrophage activation is reversible
Zhong-Yun DONG ; You-Hui ZHANG ;
Chinese Journal of Immunology 1985;0(01):-
The inhibitory effect of Fusarin C,a new mutagen isolated from Fusarium moniliforme,on M? activation was investigated.The inhibitory effect of Fusarin C disappeared partially after 24 hr culture in the absence of the mycotoxin and completely after 72 hr.In addition,it could be overcome by high concentrations of M? activating factor or anti-serum to the target cells.
4.The effects of alanyl-glutamine dipeptide-enriched parenteral nutrition on outcome in radiation enteritis rats
Guanglong DONG ; Xiaonan LIU ; Zhang ZHANG ; Yun ZHANG ; Boquan JIN
Parenteral & Enteral Nutrition 1997;0(02):-
Objective:To investigate the impact of alanyl-glutamine(Ala-Gln) on outcome in radiation enteritis rats.Methods: Male SD rats(n=70)were separated randomly into four groups: control group(n=10),AR+pseudosurgery group(n=20),AR+TPN group(n=20) and AR+TPN+Ala-Gln group(n=20).Rats were observed for mortality,changs of body weight,villous hight and area,the bcteriral translocation(BT)in mesenteric lymph nodes(MLNs),liver,spleen and peritoneal cavity.Serum TNF-? and sIL-2R level were determined by sandwich ELISA.Results: When Ala-Gln was administered in radiation enteritis rat,the mortality,body weight loss and bacterial translocation were decreaded,the villous hight and area was increased and the TNF-? and sIL-2R levels were reduced.Conclusion: Parenteral Ala-Gln nutrition can improves the results of radiation enteritis rats.
9.Feiyanning Decoction down-regulates the expressions of phosphorylated Akt and hypoxia inducible factor-1alpha proteins in transplanted lung adenocarcinoma in nude mice.
Ming ZHANG ; Zhenye XU ; Bing BAI ; Yun DONG
Journal of Integrative Medicine 2008;6(6):616-20
To observe the effects of Feiyanning Decoction, a compound traditional Chinese herbal medicine, in inhibiting the growth of transplanted A549 tumors in nude mice and the expressions of p-Akt and HIF-1alpha in A549 tumors.
10.Nursing management in the treatment of large number of infants with urinary calculi
Meixin WANG ; Sulan LIN ; Ju DONG ; Yun TANG ; Hong ZHANG
Chinese Journal of Nursing 2009;44(8):737-738
To explore the measures of nursing management in the treatment of large number of infants with urinary calculi. The nursing management measures included launching the preparedness and response project for sudden public health events, formulating scientific and standardized nursing management system,optimizing work flow,strengthening nurse training,focusing on the details in nursing management,implementing disinfection and isolation system seriously,and paying close attention to health education for the parents of minority infants. Scientific nursing management can ensure the treatment effectiveness and nursing safety for the infants with urinary calculi.