Objective To explore the effect of electroacupuncture combined with rehabilitation on P300 auditory event-related potentialsin patients with cerebral infarction. Methods 105 patients with cerebral infarction were divided into 3 groups with 35 cases in each group.The comprehensive rehabilitation group received routine rehabilitation and electroacupuncture, the general rehabilitation group only receivedroutine rehabilitation, and the control group received no intervention. They were treated once a day, 10 times as a course. P300 wasdetected before and 3 courses after treatment. Results The latency was shorter and the amplitude was higher in the comprehensive groupthan in the general group (P<0.05). Conclusion Electroacupuncture combined with rehabilitation technology can improve cognitive functionof patients with cerebral infarction.

Objective To explore the effect of electroacupuncture combined with rehabilitation technology on the recovery of motor function in patients with cerebral infarction. Methods 105 patients with cerebral infarction were divided into 3 groups with 35 cases in each group. The comprehensive rehabilitation group received routine rehabilitation and electroacupuncture therapy, the general rehabilitation group only received routine rehabilitation, the control group received no intervention. They were treated once a day, 10 times as a course.Fugl-Meyer Assessment (FMA) was used to assess the motor function after 3 courses. Results The scores of FMA were higher in both rehabilitation groups than in the control group (P<0.05), and it was higher in the comprehensive group than in the general group (P<0.05). Conclusion Electroacupuncture combined with modern rehabilitation technology could facilitate to recover motor function in patients with cerebral infarction.

Objective To analyze the social support level and its influencing factors of patients with adverse pregnancy and to provide basis for targeted nursing interventions. Methods The convenience sampling method was adopted to get 106 cases of patients with adverse pregnancy as the sample and the home-made general questionnaire and social support rating scale (SSRS) for the research were used to analyze the social support level and influencing factors of patients with adverse pregnancy. Results The overall social support level of patients with adverse pregnancy was moderate and there was no significant difference with the subjective support, objective support, support utilization rate and overall level of normal pregnant women. The education level, income and the reproductive history of patients with adverse pregnancy affected their social support. Conclusion Nurses should help patients gain more social support and help patients with poor education, low income and nulliparous adverse pregnancy better feel and use the social support.

Objective To explore the changes of S - 100? protein in cerebrospinal fluid and serum of children with viral encephalitis and its clinical significance. Methods The levels of S - 100? protein of cerebrospinal fluid and serum of 36 children with viral encephalitis and 20 lumbar anesthesia children without central nervous system diseases were measured by enzyme - linked immunosor bent assay. Differences in the levels of cerebrospinal fluid and serum S-100? protein between children with and without coma, with and without convulsion, with and without sequelae in the case group were compared. Results S-100? protein levels of cerebrospinal fluid in the case group and control group were (0.641?0.390) and (0.037 ? 0.014) ?g/L( P

Objective To explore the change of microRNA let-7b in heat acclimatization/heat stroke rat models and its relation with HSP70,IL-6,TNF-α,TGF-3 and IL-12,and analyze its clinical significance.Methods 60 male rats with almost the same anal temperature,weight,and weeks' age were selected from Laboratory Animal Center in the Second Military Medical University.They were randomly divided into control,heat acclimatization,heat stroke groups averagely.Heat acclimatization/heat stroke rat models were built in hot climate simulated animal tank,blood was collected from cordis apex and serum was separated.Real-time fluorescent quantitative polymerase chain reaction (qRT-PCR) was used to test microRNA let-7b in serum,enzyme-linked immunosorbent reaction (ELISA) was to measure the protein level of HSP70,IL-6,TNF-α,TGF-β and IL-12 in serum.Kruskal-Wallis H test was used to analyze quantitative data in the three groups,Spearman rank correlation coefficient was used to reveal relation between two variables in heat stroke group.Results M values of miRNA let-7b in control,heat acclimatization,heat stroke groups were 0.99,1.04 and 1.93 separately,Q values were 0.30,0.25 and 0.44 (x2 =38.95,P＜0.001),separately,with statistical significance.The results of pairwise comparison showed no statistical difference in control and heat acclimatization (P＞0.05),but there were differences in control and heat stroke,heat acclimatization and heat stroke groups statistically (P＜0.05).Spearman correlation analysis showed that in heat stroke group,let-7b was positively related with HSP70,TNF-α and IL-6 (rs =0.579,0.498 and 0.609,P＜0.05) with statistical significance.Conclusion miR let-7b might involve in the pathology of heat stroke,it provided a potential biomarker for monitoring patients in high temperature and humidity clinically.

Objective To analyze the affection and clinical significance of CD26/DPP4 on CD4+T cells and its cytokines in patients withCrytococcalMeningitis.Methods Peripheral blood was collected from 36 patients diagnosed withCrytococcal Meningitis in Changhai Hospital and Changzheng Hospital,Shanghai from August,2011 to December,2015,meanwhile 36 health controls’was also acquired.Peripheral blood mononuclear cell (PBMC)was separated by density gradient centrifuga-tion,CD26+CD4+T and CD26-CD4+T cell groups were classified by Flow Cytometry,the expression level of cytokines was tested by reverse transcriptase-polymerase chain reaction (RT-PCR).The correlation between DPP4 activity,CD26+CD4+T (%)and APACHE II score,IL-17,TNF-α,IL-4,IFN-γwas measured by Pearson coefficient.Results CD26+CD4+T(%)between experimental and control groups was 13.35±3.83 vs 8.39±2.14 (t=6.78,P<0.000 1).DPP4 activity was 50.89±17.21 mU/ml vs 73.83±20.24 mU/ml (t=5.18,P<0.000 1),with statistically significant differences.In ex-perimental groups,CD26+CD4+T (%)was positively related with APACHE II score,IL-17,TNF-α(r=0.431,0.564, 0.688,P=0.003 8,0.001,0.004 6).DPP4 activity was negatively interrelated with APACHE II score,IL-17,TNF-α,IFN-γ(r=-0.544,-0.489,-0.678,-0.734;P<0.001).Conclusion CD26/DPP4 may be involved in the pathogenesis of Crytococcal Meningitis through regulation of Th subgroups,and it was the potential therapeutic target and the predicted marker of the disease.

Abdominal Wall ; Adenocarcinoma ; diagnosis ; Colonic Neoplasms ; diagnosis ; Female ; Histiocytoma, Malignant Fibrous ; diagnosis ; Humans ; Ileal Neoplasms ; diagnosis ; Ileocecal Valve ; Leiomyoma ; diagnosis ; Middle Aged ; Neoplasms, Multiple Primary ; diagnosis ; Soft Tissue Neoplasms ; diagnosis ; Uterine Neoplasms ; diagnosis

OBJECTIVETo evaluate the possibility that using intracoronary delivery of autologus bone marrow-derived mesenchymal stem cells (MSCs) to improve the cardiac function after acute myocardial infarction (AMI) in miniature pig.

METHODSMSCs were cultured in Dulbecco's modified Eagle's medium-F12 (DMEM/F12) medium. AMI model was made by blocking the blood stream of the first diagonal branch in miniature pig, and released the branch after 90 minutes. After 10-14 days, (4-6) x 10(7) culture-expanded autologus 4', 6-diamidino-2-phenylindole (DAPI)-labelled MSCs were transplanted into each host heart's AMI area through intracoronary way. Ultrasonic cardiography (UCG) was performed to observe the left ventricular function at 3 months after transplantation. The cellular transplanted hearts were harvested and investigated by immunohistochemical analysis.

RESULTSLeft ventricular function of the MSCs group was improved significantly 3 months later compared with the control group [(54.65 +/- 3.39) vs (43.98 +/- 4.21)%, (P < 0.01)]. Exogenous MSCs survived and site-differentiated into cardiomyocytes in infracted hearts.

CONCLUSIONMSCs can play a benificial role to repair damaged heart. Heart function can be improved after MSCs transplantation in porcine myocardial infarction model.

Animals ; Female ; Male ; Mesenchymal Stem Cell Transplantation ; methods ; Myocardial Infarction ; pathology ; physiopathology ; therapy ; Swine ; Swine, Miniature ; Transplantation, Autologous ; Treatment Outcome

Objective To research the role of lymph tracers to protect parathyroid in surgery for papillary thyroid carcinoma.Methods Patients with papillary thyroid carcinoma who met selected criteria were enrolled in this study.Patients were divided into carbon nanoparticle group,methylene blue group,and conventional surgery group.Results No significant complication occurred in the patients of carbon nanoparticle and methylene blue groups.In carbon nanoparticle group,methylene blue group and conventional surgery group,the mean numbers of parathyroid glands detected during surgery were 3.1 ± 0.3,2.9 + 0.4 and 2.3 ± 0.3 (F =3.78,P ＜ 0.01),the rates that parathyroid was cut mistakenly were 1.37％(2/146),2.62％ (2/97) and 7.14％ (6/84) respectively(x2 =17.372,P ＜ 0.05) ; and the incidence of postoperative hypocalcemia were 10.4％ (5/48),9.1％ (3/33) and 17.5％ (7/40,x2 =0.671,P =0.037).Conclusion Thyroid lymphography technique is helpful to protect from the injury to the parathyroid glands in surgery.

OBJECTIVETo observe the effects of heat shock protein 27 (Hsp27) overexpression on doxorubicin (Dox) induced mortality and cardiac dysfunction in a transgenic (TG) mouse model.

METHODSA linear DNA constituted of alpha-myosin heavy chain (alpha-MHC) promoter, human Hsp27cDNA and poly A was microinjected into fertilized eggs to generate transgenic mice and mice containing the transgene were identified by polymerase chain reaction and independent transgenic lines were established. Following successful transmission, tissues including heart, lung, liver, brain, skeleton muscle, spleen and kidney were screened by Western blot to confirm the cardiac specific expression of the transgene. TG and wild type littermates (WT) received a single dosage of Dox injection (25 mg/kg IP) or saline injection and observed for 5 days. Mice mortality was noticed and left ventricular (LV) hemodynamics were measured at day 5 in surviving mice. Cardiomyocyte apoptosis was evaluated by TUNEL assay at day 3 post Dox or saline injections in a separate group.

RESULTSThree independent transgenic lines were generated, and all of them expressed cardiac specific Hsp27. Five days mortality was significantly reduced in TG group than that in WT group post Dox (P < 0.01), Dox induced cardiac dysfunction and cardiomyocyte apoptosis were also significantly attenuated in TG mice compared to WT mice (P < 0.05).

CONCLUSIONOverexpression of Hsp27 reduced mortality, attenuated left ventricular dysfunction and cardiomyocyte apoptosis induced by Dox in a transgenic mouse model.

Animals ; Apoptosis ; Disease Models, Animal ; Doxorubicin ; HSP27 Heat-Shock Proteins ; metabolism ; Heart Failure ; blood ; metabolism ; pathology ; Humans ; Mice ; Mice, Transgenic ; Myocytes, Cardiac ; cytology ; Oxidative Stress ; Ventricular Dysfunction, Left ; metabolism