Objective To investigate the changes of detection rates of IL-2,IL-6 and serum anti-Chlamydia immunodominant protein antibody in the patients with Chlamydia trachomatis genital tract infection.Methods Sixty women with genital tract infection (CT group) and 60 healthy women (control group) were selected in our hospital from January to May 2016.The serum levels of IL-2,IL-6 and anti-Chlamydia immunodominant protein and IL-2 and IL-6 levels in cervical secretions were detected in the two groups.Results The IL-2 and IL-6 levels of serum and cervical secretions in the CT group were significantly higher than those in the control group,the difference was statistically significant (P<0.05);the positive detection rates of serum OmcBc antibody,Pgp3 antibody,HSP60 antibody and CTT841 antibody in the CT group were 45.00%,75.00%,23.33% and 38.33% respectively,which were significantly higher than those in the control group,the difference was statistically significant (P<0.05).Conclusion The positive detection rates of IL-2,IL-6 and serum anti-Chlamydia immunodominant protein antibody in the patients with genital tract Chlamydia infection are significantly increased,which has some guiding significance for clinical diagnosis and treatment.

Objective To investigate the significance of tamoxifen in the combination chemotherapy of human multidrug-resistant bile duct carcinoma cell line (QBC939/ADM) and its mechanism. Methods The QBC939/AI)M was established, through exposure to gradually increased and the high and low alternated concentration of ADM persistently. The QBC939 and the QBC939/ADM were effected by single ADM, MMC, VDS or jointly with TAM. Drug sensitivity was measured by MTT. Growth cycle and apoptosis were performed by FCM. The level of their P-gp was detected by IHC. The content of ADM in the human cholangiocarcinoma cell line QBC939 was observed by FCM. Results The inhibitive rate of ADM, MMC, VDS to QBC939/ADM was rather lower than to QBC939. With the use of TAM, their chemotherapy effects were apparently enhanced and the apoptosis ratio increased comparably. The IHC results showed that the level of P-gp on the QBC939/ ADM was overexpressed, and the content of ADM in the QBC939/ADM group was much lower. Effected with the high content of TAM(10 μmol/L), the level of P-gp on the QBC939/ADM was decreased, with the content of ADM in the QBC939/ADM group increased comparably. TAM could both improve the chemotherapy effects of the two types of the cell, but the effect of the QBC939/ADM group was more apparent. Conclusion TAM can enhance the depressant effect of chemotherapy to both the two types of the cell, and increase the content of ADM in the QBC939/ADM group. TAM can be combined with the overexpressed P-gp.