No abstract available.
Osteoarthritis*

Sporotrichsis is a chronic granulomatous mycosis caused by Sporotrichum species. In Korea, Kim et al. had reported two cases of cutaneous sporotrichosis involving upper extremities for the first time in 1960. They had determined their strain to be similar to a variant of S.beuermanni by culture. These Kim's strain has inoculated into the peritoneum of rats pathogenicity, virulence and course by examining the macroscopic The conclusion were as follows: 1. Rats were inoculated intraperitoneally and killed at intervals of 12, 20, 30, 40 and 50 days and followed by postmortem examination. Macroscopic findings: At twelve day and lesions were observed. At 20 to 50 days all showed grayish brown lymphnodules varying in size, which were disseminated on the mesentery, omentum, peritoneum and inguinal region. On lung, liver, spleen and kidney, various changes i. e.; congestion, necrosis and degenerative changes were noted at 30 to 40 days after inoculation. 3. Microscopic findings: a. Most nodules were characterized by three distinct zones, i. e.; central suppurative, mirldle tuberculoid, and peripheral syphiloid zones. b. Visceral organs showed the following pathological changes varying with the duration of infection; diffuse hepatic necrosis, bronchitis and pneumonitis, splenitis and degene- rative changes in the kidney. c. Microorganisms could found in tissue by PAS stain, Its frequency rate shows 52% in lymphnodules and 16% in visceral organs. 4. For some unknown reasons isolation of the organisms by culture was successful only from 3 rats.
Animals ; Autopsy ; Bronchitis ; Estrogens, Conjugated (USP) ; Kidney ; Korea ; Liver ; Lung ; Mesentery ; Necrosis ; Omentum ; Peritoneum ; Pneumonia ; Rats* ; Spleen ; Sporothrix* ; Sporotrichosis ; Upper Extremity ; Virulence

With the artificially applied local inflammatory stimulus by ultraviolet irradiation to the inner aspect of the left albino rat thigh skin, the tissue mast cell changes were observed for the nerve effectiveness on the irradiated thigh (Lt.) and non-irradiated opposite thigh (Rt.) and tongue. Using the distilled water (for control group), Bistrium Bromide blocking agent), Yhenobarbital Sodium (sedative and hypnotic) and (autonomic ganglion Reserpine (tranquillizer) were regularly subcutaneously injected every 8 hours, and the following results of most cell changes were observed. 1) Without ultraviolet ray irradiation, the rats of first group were received distilled water and average number of mast cells at the Rt. and Lt. inner aspects of thigh and tongue were gradually decreased and 24 hours after injection, the number of each part showed lowed curve, that was 85. 1%, 84. 8% and 91. 8% of normal average number. On the second and 4th day of the injection, increasing number of each part reached to the normal average number. The 7th day of injection maximum number of above average was l02.1%, 110.8% and 110.7% 2) With the ultraviolet ray irradiation the rats of second group received distilled water. At the Rt, and Lt. inner aspects of the thigh and tongue, the average number of mast cells gradually decreased and on the 4th day of the injection, the lowest number was 59.3,"o, 78.7% and 86. 2% of each average number. However on the 7th day the increasing number showed 75.0%, 84.6% and 89.6% of average number. 3) Group 3; Received Bistrium Bromide injection respectively, 24 hours after injection at the Rt. and Lt. inner aspects of the thigh the gradually decreasing number of mast cells showed the lowest curve, it accounted 70.6% and 74.0% of normal value. However at the tongue it was 77.2% on the second day of injection. Their highest number was seen on the 7th day, it was 95.7%, 100.5% and 107. 8% respectively 4) Group 4; Received Bistrium Bromide and ultraviolet ray irradiation, the average number of mast cells at the Rt. and Lt. inner aspects of the thigh and tongue showed decreasing curve. On the second day of injection the lowest number was 43.3%, 65.4% and 74.9/o of normal value respectively. On the 4th day of injection and irradiation the average number was increased, then after 7th day of injection maximum value was 93. 7%, 92.1% and 115.1% of normal number. 5) Group 5; Received Phenobarbital Sodium, the average number of mast cells at the Rt. and Lt. inner aspects of the thigh and tongue was gradually decreased, the lowest number was seen on the 24 hours after injection, it was 76.7%, 8.7% and 90. 9% of normal value. On the second and 4th day of injection, the curve was increased and on the 7tIi day, the highest value was 106.5%, 104.8% and 104. 6% of normal average value. 6) Group 7; Received Phenobarbital Sodium with ultraviolet rayirradiation at the Rt. and Lt. inner aspects of the thigh and tongue, the average number of must cells were gradually decreased. On the 4th day of injection and irradiation, they reached to the lowest number, and it was 43.3%, 80.6% and 74.0% of normal number. On the 7th day it showed the highest value of 74.9%, 97.2% and 95.8% respectively. 7) Group 7; Received Reserpine at the Rt. and Lt. inner aspect; of the thigh and tongue, the number of mast cells gradually decreased. On the 4 hours after injection 85.4%, 84.0% and 87.9% of average normal number was seen. On the second day, respectively number was increased. On the 7th day maximum increasing number was 103.1%, 115.2% and 95.0% of normal average number 8) Group 8; Received Reserpine and ultraviolet ray irradiation, the decreasing number of mast cells reached to the lowest value on the 4th day after injection, it was 53.5%, 80.3% and 84. 4% of normal value. On the 7th day of procedure, increased number showed 94.8%, 110.3% and 92.9 % of normal value. From the above experimental study, Ristrium Bromide, Phenobarhital Scdium and Reserpibne gave some effect to the change of mast cell at the irracliaticn area by ultraviolet ray and non-irradiated distant part.
Animals ; Ganglion Cysts ; Mast Cells* ; Phenobarbital ; Rats ; Reference Values ; Reserpine ; Skin ; Sodium ; Thigh ; Tongue ; Ultraviolet Rays ; Water

No abstract available.
Delivery of Health Care* ; Democratic People's Republic of Korea*

No abstract available.
Thoracic Surgery, Video-Assisted*

No abstract available.
DNA*

Hantavirus is a genus of the Bunyaviridae family causing two serious diseases, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Puumala virus is a member of hantavirus originally found in Europe, and its natural reservoir is Clethrionomys glareolus. It is also associated with the hurnan disease nephropathia epidemica, a milder form of HFRS. To identify the hantaviruses in bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area in Korea, and nested RT-PCR was performed with serotype specific primer from M segment. Interestingly, Puumala virus was detected in bats (Rhinolophus ferrum-equinum) only from Won-Joo. The 327 bp nested RT-PCR product, was sequenced. The sequence database search indicates that the sequence is homologous to the published sequence of Puumala viruses. The sequence similarities were ranged from 71% to 97%. The highest sequence similarity was 97% with Puumala virus Vranicam strain, and the lowest was 71% with Puumala virus K27 isolate. Puumala virus Vranicam strain was isolated from a bank vole (Clethrionomys glareolus) in Bosnia-Hercegovina. Puumala virus K27 was isolated from human in Russia. This analysis confirms that bats (Rhinolophus ferrum-equinum) in Korea are natural reservoir of Puumala virus.
Arvicolinae ; Base Sequence ; Bunyaviridae ; Chiroptera* ; Chungcheongbuk-do ; Europe ; Hantavirus ; Hantavirus Pulmonary Syndrome ; Hemorrhagic Fever with Renal Syndrome ; Humans ; Korea* ; Puumala virus* ; Russia ; Sequence Analysis*

Hantavirus is a genus of the Bunyaviridae family consisting following serotype groups: Hantaan, Seoul, Puumala, Prospect Hill, Thailand, Belgrade, Thotta palayam, Sin Hombre. Most of Hantavirus group have been associated with many clinically similar disease known collectively as hemorrhagic fever with renal syndrome (HFRS). Hantaan virus is the prototype of the genus hantavirus, originally isolated from Apodemus agrarius. Bat was found as a natural host for Hantaan virus in Lee's lab for the first time. Then, Hantaan-like virus was isolated Hantaan-like virus from bat. To identify hantaviruses that are present in Korea among bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area, RNA was isolated from lung and serum. RT-PCR was performed with a universal primer from M segment. Nested RT-PCR was carried out to differentiate Hantaan, Seoul and Puumala virus using serotype specific primers. As we expected, Hantaan viruses were detected in bats and Seoul virus was not detected. Interestingly, Puumala viruses were also detected in bats from won-Ju, but not in other areas. Puumala virus is originally isolated from Clethrinomys glareolus, and cause light HFRS. Recently, Paradoxomis webbiana, a wild bird turn out to be a reservoir for Puumala virus in Korea. These data indicate that bat is a new natural reservoir of Puumala virus.
Animals ; Birds ; Bunyaviridae ; Chiroptera* ; Chungcheongbuk-do ; Gangwon-do ; Hantaan virus* ; Hantavirus ; Hemorrhagic Fever with Renal Syndrome ; Humans ; Korea* ; Lung ; Murinae ; Puumala virus ; RNA ; Seoul ; Seoul virus ; Thailand

No abstract available.
Pancreatic Pseudocyst*

Basal cell carcinomas(BCCs) are usually treated with ablative proccdures, but a nonsurgical treatment would conserve tissue and obtain a better cosmetic out come as well as treat patients who refuse surgery or those who are not good surgical candidates. Because of inadequate drug penetration, low cure rates, high recurence rates, and adverse reactions, such as contact dermatitis, topical 5-fluorouracil(5-FU) is unsaitble to treat nodular BCCs. But, int,ralesional 5-FU has been reported to be effective for nodular BCCs. The efficacy and safety of intralesional injection of 5-FU have been evaluated for treatment of BCCs. The results revealed that intralesional injection of 5-FU seems to be effective in the treatment of various premalignant and malignant skin lesions as well as BCCs without side effects.
Carcinoma, Basal Cell* ; Dermatitis, Contact ; Fluorouracil* ; Humans ; Injections, Intralesional* ; Skin