Objective: To investigate the protective effect of deferoxamine preconditioning against hypoxia injury in astrocytes and the underlying mechanisms. Methods: Astrocytes were cultured under ischemia stress, which was mimicked by oxygen and glucose deprivation (OGD) with deferoxamine. Astrocytes were divided into three groups: normally cultured group; Deferoxamine pretreated group: astrocytes were pretreated with Deferoxamine and then treated with Deferoxamine OGD; and OGD group; astrocytes were treated with Deferoxamine OGD. The cell viability examination, the ratio of condensed nuclei, and the morphological changes were used to assess the protective effect of deferoxamine. The expression of HIF-1a and EPO protein and mRNA in astrocytes was examined by immunofluorescence staining and RT-PCR, respectively. Results: The morphology of AS in the deferoxamine pretreated group kept intact. AS viability in deferoxamine pretreated group was 58% of the normally cultured group, and that in the OGD group was 25% of the normally cultured group (P<0. 05). The ratio of condensed nuclei in deferoxamine pretreated group was 38% and that in OGD group was 30% (P<0. 05). Immunofluorescence staining found that expression HIF-1a, and EPO protein appeared in cultured astrocytes after deferoxamine pretreatment. RT-PCR confirmed that mRNA of HIF-1a and EPO was up-regulated after deferoxamine pretreatment. Conclusion: Deferoxamine preconditioning can protect the astrocytes from hypoxia damage, which is related to deferoxamine-induced increase of HIF-1a and EPO expression.

AIM: To investigate the difference of cognitive impairment and depression between age-related macular degeneration ( AMD ) group and the control group patients. ·METHODS: A prospective case-control study was performed from November 2014 to August 2016 in the hospital for AMD patients and sex-matched control group. The Mini-Mental State Examination ( MMSE) and the Geriatric Depression Scale ( GDS ) score of each patient were collected for statistical analyzing. ·RESULTS: There were total 84 cases ( 168 eyes ) included in the study. The difference of visual acuity between the two group was statistically significant ( F=8. 953, P=0. 004) by baseline data analyzing. There were no significant differences in MMSE scores between the two groups according to educational status ( P>0. 05 ) , while the prevalence of cognitive impairment in each group was statistically significant (x2 =4. 14, P=0. 042). The difference of GDS scores, prevalence of total and mild depression between two groups were both statistically significant (F=5. 852, P=0. 018; x2=6. 372, P=0. 012; x2 = 5. 674, P = 0. 017 ). However, there was no statistically significant difference in the prevalence of moderate to severe depression (x2=0. 672, P=0. 412). ·CONCLUSION: AMD patients have a higher prevalence of depression. Although MMSE score differences were not statistically significant in subgroup analysis by educational levels, AMD patients are more likely to have cognitive impairment overall.

Objective To investigate the distribution of pathogenic bacteria of vaginal discharge in bacterial vaginosis.Methods The results of bacterial culture and drug sensitive tests of vaginal discharge from patients with bacterial vaginosis were analyzed.Results The positive rate of bacteria culture of vaginal discharge was 79.3%(115/145).The dominant bacteria were staphylococcus epidermidis 27.0%(31/115),staphylococcus intermedius and staphylococcus aureus 13.0%(15/115),which were obviously higher than other germs.The drug sensitive tests showed that staphylococcus were relatively sensitive to vancomycin,fosfomycin,amikacin and rifampin.But the drug resistance to penicillin,tetracycline,erythromycin and oxacillin was the highest.Conclusion The kinds of pathogenic bacteria in vaginal discharge are various.The main bacterium is staphylococcus,and drug resistance is very severe.The isolation and drug sensitive test of pathogenic bacteria play an important role in diagnosis and treatment of gynecological disease.

Objective: To optimize the preparation for Xinjianwei Tablet, and to establish a method for the quantitative determination of hesperidin. Methods: The new process used dried tangerine peel raw powder as medicine to replace the traditional water decocting process. The effect of the new process was verified with the content of hesperidin as evaluation index. Results: The new process is superior to the traditional one on the premise of the basic substances and the total yield did not change basically. RP-HPLC was used to determine hesperidin and the linearity was good in the range of 10.1-202.5 μg/mL, r = 0.9999. The average recovery was 98.37% with RSD = 1.0%. Conclusion: The improved process is more rational and simple, and the method based on HPLC is simple, reliable, and reproducible. It could be used effectively for the quality control of Xinjianwei Tablet.

AlM: To evaluate the clinical effect of 200g/L protein-free calf blood extract eye gel for corneal epithelial defect.METHODS: One hundred and sixty - eight cases of corneal epithelial defect ( 58 cases with herpes simplex keratitis; 24 cases with chemical injury; 85 cases with pterygium operation injury ) were randomly divided into two groups: 84 eyes were treated with protein-free calf blood extract eye gel; 84 cases were treated with basic fibroblast growth factor eye gel ( bFGF ) . The bFGF and protein-free calf blood extract eye gels were used 4 times a day. The treatment course was 7d. Epithelial defect restoration, local symptom and sign were observed.
RESULTS: The difference between pre-treatment and post-treatment was significant ( P<0. 01 ) in the two groups. The effect of protein-free calf blood extract eye gel (83. 3%) group was prior to that of bFGF (69%) for corneal epithelial defect. The effective rate of protein-free calf blood extract eye gel in the herpes simplex keratitis, chemical injury and pterygium operation injury was 72. 4%,69. 2% and 95. 2%. Localized stimulus and adverse reaction of all over the body were not been observed.
CONCLUSlON: Protein-free calf blood extract eye gels is valuable and safe for corneal epithelial defect.

OBJECTIVE: To investigate the application of autogenous cartilage transplantation in rhinoplasty. METHOD: We chose three kinds of treatment according to the shape of nasal tip and thickness of local soft tissue. Autogenous auricular cartilage transplantation combined with "L" type artificial prosthesis rhinoplasty was executed in 57 cases. Nasal alar cartilage transplantation combined with "L" type artificial prosthesis rhinoplasty was executed in 33 cases and septal cartilage transplantation combined with "willow leaf" type artificial prosthesis rhinoplasty was executed in 29 cases. RESULT: Improved nasal aesthetic effects were observed after operation in all of 119 cases, 64 cases were follow-up visited for 3 to 12 months. Both surgeons and patients were satisfied with the nasal shape. CONCLUSION Autogenous cartilage transplantation combining with artificial prosthesis rhinoplasty could effectively rebuild the nasorostral shape. We chose different kinds of cartilage according to the nasorostral condition. We can ensure that the whole nasal shape according to aesthetic requirement.
Adult ; Female ; Humans ; Male ; Middle Aged ; Nasal Cartilages ; transplantation ; Rhinoplasty ; methods ; Transplantation, Autologous ; Young Adult

Objective: To detect methylation status of p16 CDKN2A exon 1 during experimental carcinogenesis in rats. Methods:Thirty male clean SD rats were fed with 0.02 g/L of 4-nitroquinoline-oxide (4NQO) in drinking water.13, 16 and 24 weeks after experiment the normal, moderate-severe dysplasia and invasive squamous cell carcinoma tissues were removed from their tongues respectively; then the methylation status of p16 CDKN2A exon 1 were detected by methylation-specific PCR(MSP). Results:A 123 bp-unmethylated product was amplified in all samples but the methylated product was not detected in any of the samples. Conclusion: The p16 CDKN2A exon 1 appeares to be unmethylated during carcinogenesis of tongue cancer in experimental rats.

Objective:To develop an interface program between a hospital inspection information system and Hospital Information System(HIS). Such interface program not only guarantees the safety and independence of HIS, but achieves interoperability between HIS and other third-party system.Methods: The interface program between the inspection information system and HIS conforming to hospital demands is developed by using Visual Studio 2008 development platform and WebServices+XML technology.Results: The interface between the inspection information system and HIS operates stably, reliably and efficiently, avoiding manually input information errors, reducing the amount of registration work, and raising work efficiency.Conclusion:WebServices+XML technology is utilized to develop an interface characterized by safety, stability and reliable performance, realizing resource sharing among information systems in the hospital. Besides, the interface can be expanded in the future to adapt to other third-party system, achieving customization.

·α-crystallin is the predominant structural protein in the lens.lt is a member of small heat shock proteins ( sHSPs) which has the common functions of HSPs.lt also has anti-apoptotic activity etc.Recently, it has been proved to combine with the cellular membrane of retinal ganglion cells ( RGCs ) to enhance the survival of RGCs and the regeneration of axons, thereby partly restore visual function.But we haven’t come to a unified conclusion of the mechanism.This review is focused on structure and functions of α-crystallin, the protection function and mechanism of α-crystallin towards RGCs after the optic nerve injury.

Objective To explore the diagnosis and curative effect of phenylketonuria(PKU) in Gansu.Methods Fifty-nine thousand nine hundred infants and 118 borderline cases were screened from Oct.1999 to Dec.2006.Three drops of venous blood was collected from the exterior or interior part in the heel,and trickled the venous blood onto the specialized filter paper,which could be formed into 3 bloodstains with the diameter of 0.8-1.0 cm.Phenylalanine(phe) was detected by chemiluminescence method,and PKU was screened by the PKU standard of diagnosis.Infants with PKU had been given low phe food.During the treatment,phe level,physical development and intellectual development were measured regularly.Patients′ body weight,height and head circumference were measured once 1 month.Patients′ intellectual quotient(IQ) were measured every half year(IQ was analyzed by Gesell test).Results were analyzed by SPSS 11.0 software for t test.Results In total,93 patients with PKU were diagnosed and treated.Thirty-six cases were identified by neonatal screening before 3 mouths old.Mental retardation was found in 57 cases before 6 years old.After treatment with low-phe diet,the follow-up for early-treated patients revealed that their physical and mental development were normal,and height,weight and head circumference were in the normal amplitude,and the results of IQ was(92?12)scores.In later treated patients,abnormal behaviors were significantly improved and their developmental quotients were elevated.Before treatment,Gesell value was(57?20)scores;after 12-18 months treatment Gesell value was(70?20)scores.The difference was significant(t=1.705 P