Two monoclonal antibodies of anti-mouse pancreatic cancer, 2B_6and 2B~(10), were purified by the use of salt fractionation-exchange chromatography(DEAE-52) or affinity chromatography from culture supernatants and mice ascites of the hybridomas. After assayed by the use of SDS-PAGE and labeled with FITC, they were used to analyse related antigen site of 35 tissue samples of several human tumor by means of direct-fluorescence method. There was an intense positive reaction between 2B_(10)and 4 samples of gastric cancer. 2B_6or 2B_(10)and 3 tissur samples of ovary cancer and 1 clonal cell of ovary cancer also had intense positive reaction. These results showed that there exist common tumor antigen in both human and mice.

According to the requirement of subject structuring and specific development,this paper combine with the teaching experience of fermentology course to make a preliminary discussion about how to reform teaching material、teaching method and practice content.

Objective: To explore the relationship between coxsackie-adenovirus receptor(CAR)expression and the infection efficiency of adenovirus vector in gene therapy for hepatocellular carcinoma through regulating CAR expression on cells surface via inhibition of the Raf/MEK/ERK pathway.Methods: Western blotting analysis was used to examine CAR expression in hepatocellular carcinoma cells(SMMC-7721 and HepG_2)before and after treatment with U0126,inhibitor of Raf/MEK/ERK signal transduetion.SMMC-7721 and HepG_2 were infected by a non-replicating,E1 A-deleted adenovirus expressing EGFP(Ad-GFP).FACS was used to analyze the infection efficiency of Ad before and after U0126 treatment. Results: The expression of CAR on cell surface had an increasing tendency after treatment with U0126.FACS analysis showed significantly increased infectivity of cells treated with the MEK inhibitor U0126 compared with untreated cells: SMMC-7721,(71.65?6.21)%→(86.54?5.70)%,HepG_2,(77.53?4.62)%→(87.06?2.83)%,when infected with Ad-GFP at the same MOI(10 MOI).Conclusion: The inhibition of Raf/MEK/ERK pathway by U0126 may up-regulate the expression of CAR in some hepatocellular carcinoma cells,which subsequently enhances the susceptibility of adenovirus infection to target cells.

The article describes the characteristics of the mode of how to strengthen the construction of the intensive curricula.It gives some ideas according to existing problems.

Objective To investigate the effect of CD147 monoclonal antibody (mAb) on the natural resistance to paclitaxel (TAX) in the human cervical cancer line (HCE1) multicellular spheroid (HCE1/MCS) model and if CD147 mAb can reverse the HCE1/MCS resistance to TAX. Methods HCE1/MCS was obtained by liquid overlay and rotating technique. HCE1/MCS morphological changes were observed before or after the interference of CD147 mAb. The effects of TAX on HCE1/MCS (including inhibition ratio, IC50 and index of multicellular resistance) before or after CD147 mAb treatment were determined by the method of WST-1 and the inhibition ratio curve was mapped. Cell cycle and apoptosis were detected by flow cytometer (FCM). The expression of CD147 and P-gp of both HCE1/MC and HCE1/MCS was detected by immunocytochemistry. Results HCE1/MCS was established successfully. CD147 mAb could inhibit HCE1/MCS from forming spheroids. CD147 mAb could enhance the sensitivity of HCE1/MCS to TAX. IC50 in different concentrations of CD147 mAb (5,10,20 μg/mL) HCE1/MCS group were (40.31±3.73), (32.43±1.56), and (30.69±1.01) μg/mL. CD147 mAb resulted in G1/G0 arrest in HCE1/MCS. CD147 mAb of low concentrations (0-10 μg/mL) caused a dose-dependent inhibition of HCE1/MCS (P<0.05). Combined with TAX, CD147 mAb could also induce HCE1/MCS cell cycle arrest in both G1/S and G2/M stage. The expression of CD147 and P-gp was consistent in HCE1/MCS groups. Conclusion CD147 plays an important role in muliticellular resistance of cervical cancer and inhibition of CD147 can synergistically reverse the multicellular drug resistance (MCR) in cervical cancer. The MCR of HCE1/MCS mediated by CD147 is related to P-gp.

Objective To find out the hygienic status of rural drinking water in Jianhe County of Guizhou Province.Methods Forty-eight source water and tap water samples from small centralized water supply stations in 12 townships of Jianhe County in dry season and wet period were tested from 2009-2011.The water samples were examined in accordance with the relevant provision of Standard Examination Methods for Drinking Water (B/T5750-2006).The contents included:①sensory indicators:standard color,turbidity,smell and taste and visible objects; ②general chemical indicators:pH,iron,manganese,chloride,sulfate,total dissolved solids,total hardness,oxygen consumption and ammonia; ③toxicological indicators:fluoride,arsenic and nitrate; ④microbial indicators:total number of colonies,with a total population of Escherichia coli and heat-resistant Escherichia coli Outcome evaluation was carried out in accordance with the Drinking Water Health Standards (B/T 5.749-2006).Results A total of 192 rural water samples were collected in 2009-2011,and 18 samples were qualified,accounting for 9.38％.The differences of water passing rate between groups of years were statistically significant (x2 =14.74,P＜ 0.01).Rural drinking water quality in dry season (18.75％,18/96) was better than that in wet season (0.00％,0/96; x2 =19.76,P ＜ 0.01).Passing rate of source water quality (16.67％,16/96) was higher than that of tap water(2.08％,2/96; x2 =11.95,P ＜ 0.01).Sensory indicators and toxicological indicators of 192 water samples were qualified.General chemical indicators:in addition to four water samples with pH exceeded the standard(two copies in 2009 and two copies in 2010),other test indicators were qualified.Microbiological indicators:passing rates of the total number of colonies,Escherichia coli group and heat-resistant Escherichia coli group were 77.08％ (148/192),9.90％ (19/192) and 20.31％ (39/192),respectively.Passing rate of microbes was 18.75％ (18/96) in dry season and 0.00％ (0/96) in wet period,and microbes passing rate was significantly higher in dry season than that of the wet period(x2 =19.76,P ＜ 0.01).Passing rate of microbes was 16.67％(16/96) in source water and 2.08％(2/96) in tap water,and passing rate of the source water was significantly higher than that of the tap water(x2 =11.95,P ＜ 0.01).Conclusions The hygienic status of rural drinking water in rural areas of Jianhe County of Guizhou Province is poor.Microbial pollution is the main reason.

AIM: To explore the relationship between different timing of vitreous surgery and prognosis after the open globe injuries.
METHODS:Fifty-eight cases (58 eyes) with open globe injuries were chosen in our hospital from June 2011 to June 2013, and randomly divided into observation group and control group to have vitreous surgery in early phase and normal time respectively. Thirty cases ( 30 eyes ) in the observation group were received vitreous surgery during 3d, and 28 cases (28 eyes) in the control group from one to two weeks. The vitreous surgery of two groups of patients were finished by the author herself. The ocular injury before surgery, the effect of surgery treatment and the incidence of complications after surgery of the two groups of patients were compared.
RESULTS: The eye damage situation such as lens rupture, vitreous hemorrhage, retinal detachment and corneal laceration of two groups of patients before surgery were no significant statistical difference ( P >0.05). The postoperative therapeutic effect of observation group was better than the control group (χ2=6. 869, P<0.05 ); the postoperative complications of observation group was obviously lower than the control group (P<0. 05). CONCLUSION: The timing of vitreous surgery of patients with open globe injuries correlate with the prognosis, which prompts that better therapeutic effect can be achieved and the occurrence of complications can be reduced in early vitreous surgery of the patients with open globe injuries.

Objective To study the curative efficacy of mometasone furoate aqueous nasal spray combined with loratadine in the treatment of pediatric allergic rhinitis, and its effects on IL-10, IL-17 in serum.Methods 126 patients of pediatric allergic rhinitis who were admitted in our hospital from May 2011 to September 2013 were selected as research objects and randomly divided into treatment group and control group, 63 patients in each group.The control group were treated with loratadine, while the treatment group were treated with mometasone furoate aqueous nasal spray in combination with loratadine.The curative efficacy, scores of clinical symptoms and nasal examination, curing time, medication time and serum IL-10, IL-17 levels were compared between the two groups.Results The total therapeutic efficacy ratio in the treatment group was 98.41%, which was statistically higher than that of 80.95% in the control group (P<0.05).5 weeks after the treatment, scores of sneezing, runny nose, nasal congestion, nasal itching, eye itching and nasal examination in the treatment group were statistically lower than those in the control group (P<0.05).The curing time and medication time in the observation group were statistically shorter than those in the control group ( P <0.05 ) .The inflammatory factors, in comparison with the control group after treatment, the treatment group had statistically higher level of serum IL-10 and lower level of serum IL-17 ( P <0.05 ) . Conclusion Mometasone furoate aqueous nasal spray in combination with loratadine is effective for pediatric allergic rhinitis with a high therapeutic efficacy ratio.And it could significantly improve clinical symptoms, shorten treatment time, increase level of IL-10 and reduce level of IL-17.

Objective The aim of this prospective randomized study was to compare the effects of remifentanil (R) and fentanyl (F) given by target-controlled infusion (TCI) on the Cp50 of TCI propofol for loss of consciousness ( LOC ) . Methods Sixty-four ASA 1 or II patients aged 20-55 yr undergoing elective cholecystectomy or mastectomy under general anesthesia were enrolled in this study. Their BMI ranged from 18-30 kg?m-2. The patients were randomly allocated to one of four groups with 16 patients in each group: (1) propofol alone (P), (2) P + remifentanil (Cp = 4 ?g?L-1 ) (R4), (3) P + remifentanil (Cp = 7 ?g?L 1 ) (R7) and (4) P + fentanyl (Cp = 4?g?L-1 ) (F). The patients were unpremeditated. Anesthesia was induced with remifentanil or fentanyl and propofol both given by TCI. The plasma concentration (Cp) of remifentanil and fentanyl were fixed in each group. The Cp50 of propofol for LOC was determined by up-and-down sequential trial. Cp of propofol was set at 1.25, 1.50, 1.80, 2.16, 2.59, 3.11, 3.73 and 4.48 mg?L-1 . If a patient did not go to sleep at a certain Cp of propofol, the next patient was tested at a higher concentration conversely if the patient went to sleep a lower concentration was tested in the next patient. The BIS values and hemodynamic changes were recorded before induction and at LOC (no response to verbal command and loss of eyelash reflex). The TCI pump was controlled by pharmacokinetic models developed by Marsh (propofol) Minto ( remifentanil) and Shafer ( fentanyl) . Results The Cp of propofol for LOC in group P was 3.48 mg ? L-1 , significandy higher than that in group F (2.31 mg ? L -1 ), group R4 (2.11 mg?L-1) and group R7 (1.76mg?L-1 ) (P

Pre-implantation Genetic Diagnosis(PGD) is an advanced technology based on in-vitro fertility-embryo transfer,which binds multiple-discipline arts,specially the research on DNA analysis assay of single cell,to reach the aim of aristogenesis and wash out abnormal embryo before transfer.With the development of this technique and the ever wider applications,ethical issues also arise.This paper focuses on these ethical issues related to PGD,expecting an actual benefit for human.