No abstract available.
Humans ; Specialization*

Since the first report on the blood triglyceride level of normal korean in 1962, more than 12 authors published their normal value ranging from 78.0~151.0mg% as mean value. In an attempt to establish normal triglyceride level in Korean, the timing of sampling and method of sample preservation were reevaluated in terms of quality control. Although the number of samples and tests were too small to make an unequivocal conclusion, the following results were observed. 1. Plasma triglyceride levels were stable on continuing fasting 12~16 hours, slightly decreased thereafter. 2. Samples kept in room temperature were stable up to 3 days and the triglyceride level dropped moderately on the 5th day. 3. No significant change in plasma triglyceride level was found with freezing up to one month. 4. Significant change in plasma triglyceride level was noted with repeated melting and refreezing of specimen. 5. The probable reasons for marked differences in normal value of plasma triglyceride level were discussed.
Fasting ; Freezing ; Plasma* ; Quality Control ; Reference Values ; Triglycerides*

STUDY DESIGN. This is a retrospective study. OBJECTIVES. To verify the advantage of segmental pedicle screw fixation in restoration of thoracic kyphosis in hypokyphotic patients. SUMMARY OF BACKGROUND DATA. Restoration of thoracic kyphosis is essential for a satisfactory three dimensional correction in thoracic AIS. However, with significant preoperative hypokyphosis, the result of multiple hook fixation was often unsatisfactory. METHODS. Fifty-one patients with thoracic AIS treated by posterior instrumentation and followed up for more than 2 years were divided into 3 groups each comprising 17 patients. Hypokyphosis- Hook(HH) group comprised patients with thoracic kyphosis less than 15degrees treated by multiple hooks. Hypokyphosis-Screw(HS) group comprised hypokyphotic patients treated by segmental pedicle screws. Normal kyphosis-Screw(NS) group comprised patients with kyphosis greater than 15degrees treated by segmental pedicle screws. Preoperative thoracic kyphosis was 4.1+/-8.6degrees, 8.1+/-5.6degrees and 27.3+/-9.8degrees in the HH, HS and NS group respectively. There was no significant difference between the HH and HS groups(p=0.39). Three groups were compared for restoration of thoracic kyphosis. Results. At the final follow up, the thoracic kyphosis was 14.5+/-10.2degrees, 27.3+/-11.3degrees and 28.3+/-3.7degrees in the HH, HS group and NS group showing correction of 10.0+/-10.0degrees, 19.2+/-14.0degrees and 0.4+/-12.3degreesrespectively. There was a significant difference between HH and HS group(p=0.0008). However there were no difference between tile HS and the NS group(p=0.8). CONCLUSIONS. Segmental pedicle screw fixation was more effective than multiple hooks in restoring the thoracic kyphosis in AIS patients with hypokyphosis. The restored kyphosis was similar to that obtained in patients without hypokyphosis.
Adolescent* ; Follow-Up Studies ; Humans ; Kyphosis* ; Retrospective Studies

BACKGROUND: Accumulating evidence shows that interleukin(IL)-1 plays a critical role in inflammation and connective tissue destruction observed in both osteoarthritis and rheumatoid arthritis. IL-1 induces gene expression related to cytokines, chemokines and matrix metalloproteinases by activation of many different transcription factors. MATERIALS AND METHODS: The chondrosarcoma cell line, SW1353, is known to be a valuable in vitro system for investigating catabolic gene regulation by IL-1beta in chondrocytic cells. To explore and analyze the changes in gene expression by IL-1 responsible for arthritis, SW1353 was treated with IL-1 for 1, 6 and 24 h and then total RNAs were purified for each time. The changes in gene expression were analyzed with 17k human cDNA microarrays and validated by semi-quantitative RT-PCR. RESULTS: Greater than a two-fold change was observed in 1,200 genes including metallothioneins, matrix metalloproteinases, extracellular matrix proteins, antioxidant proteins, cytoskeleton proteins, cell cycle regulatory proteins, proteins for cell growth and apoptosis, signaling proteins and transcription factors. These changes appeared to be correlate with the pathophysiological changes observed in early osteoarthritis. CONCLUSION: cDNA microarray analysis revealed a marked variability in gene expression, and provided insight into the overall molecular changes. The result of this study provide initial information for further studies to identify therapeutic targets in osteoarthritis pathogenesis.
Apoptosis ; Arthritis ; Arthritis, Rheumatoid ; Cell Cycle Proteins ; Cell Line ; Chemokines ; Chondrosarcoma* ; Connective Tissue ; Cytokines ; Cytoskeleton ; DNA, Complementary* ; Extracellular Matrix Proteins ; Gene Expression ; Humans* ; Inflammation ; Interleukin-1 ; Interleukin-1beta ; Interleukins* ; Matrix Metalloproteinases ; Metallothionein ; Microarray Analysis ; Oligonucleotide Array Sequence Analysis* ; Osteoarthritis ; RNA ; Transcription Factors

The activation of neurotrophic signaling pathways following the upregulation of glial cell line-derived neurotrophic factor (GDNF), a member of the transforming growth factor-β family, has a potential neuroprotective effect in the adult brain. Herein, we report that hippocampal transduction of adeno-associated virus serotype 1 (AAV1) with a constitutively active form of ras homolog enriched in brain [Rheb(S16H)], which can stimulate the production of brain-derived neurotrophic factor (BDNF) in hippocampal neurons, induces the increases in expression of GDNF and GDNF family receptor α-1 (GFRα-1), in neurons and astrocytes in the hippocampus of rat brain in vivo . Moreover, upregulation of GDNF and GFRα-1 contributes to neuroprotection against thrombin-induced neurotoxicity in the hippocampus. These results suggest that AAV1-Rheb(S16H) transduction of hippocampal neurons, resulting in neurotrophic interactions between neurons and astrocytes, may be useful for neuroprotection in the adult hippocampus.

A total of 28 adult cases who were confirmed cysticercosis with or without cerebral involvements were treated with praziquantel at the daily dose of 3 x 25mg/kg for 3 to 7 consecutive days and was evaluated for tolerance and therapeutic effects in the trials clinically performed.The assessment of drug efficacy of praziquantel in the dermal cysticercosis was made by comparing the numbers of cysticercus nodules and histopathological findings of the biopsied parasites by means of light, scanning and transmission electron microscope. In the cerebral cysticercosis, the assessment was considered by the frequency of the episodes of convulsive seizure before and after treatment with praziquantel and by the findings of the disapearance or decreased densities of the lesions in C.T. scan in comparison with those of before and after treatment. The results were as follows: The cysticerci in the subcutaneous tissues began to disappear within one month of drug administration of 3 x 25mg/kg praziquantel over 3 to 7 days. Within 3 to 6 months most of the cysticerci had disappeared, although in some case a small number of cysticercus nodules remained even one year after treatment. Histological observation of the cysticerci biopsied at different times during the course of treatment revealed that morphological changes were already taking place within two weeks after the treatment. At the early stage of the treatment, small vacuoles were scattered along the basement layer in the tegumental syncytium of the scolex and neck regions. In the scanning electron microscopic observation, marked surface changes were present in the neck region with many bleb-like structures formed by the bursting of the large vacuoles in the tegumental syncytium. In the specimens biopsied at 2 or 5 weeks after treatment, the degenerations and necrosis of the tegumental syncytium were seen in all parts of cysticercus. In 12 cases of cerebral cysticercosis treated with praziquantel at the daily dose of 3 x 35 mg/kg for 3 or 4 consecutive days, there were no ceasing of the convulsive seizures during the 6 months follow-up. Among them 9 cases were given again the same doses of the drug for 4 or 7 days. In 7 of 9 cases, no more convulsive seizure was experienced over one or two years after the second time. At the same treatment the lesions of the brain C.T. scan disappeared, decreasd in size or calcified after treatment. In other 3 cerebral cysticercosis cases, complete cure was also obtained after the oral medication of praziquantel at the daily dose of 3 x 25 mg/kg for 7 consecutive days. In the treatment of cerebral cysticercosis with praziquantel, it was found that the concomitant oral medication of dexamethasone during the course of treatment was effective for preventing and minimizing the side-effects.
parasitology-helminth-cestoda ; Taenia solium ; cysticercus ; cysticercosis ; brain ; dermis ; chemotherapy ; praziquantel

BACKGROUND: Despite growing awareness of health behavior, making actual behavioral change seems to be more complex. This study assessed factors influencing discrepancy between the awareness and health behavior in Korean middle-aged people. METHODS: A random-digit-dial telephone survey of 1,047 middle-aged Koreans was carried out in 2004. Through the survey, the agreement between the level of awareness and health behavior was analyzed. And factors related to health-risk behaviors in the group of appropriate awareness were analyzed. RESULTS: In the group of appropriate awareness, males tended to be smokers, problem-drinkers and were not maintaining normal weight. In men, younger age, longer work-hours and more frequent stress were significantly related to both smoking and problem-drinking despite appropriate awareness. Higher income and blue-color working class were also predictors for the problem- drinking. In women, older age, lower education, lower income and comorbid disease were significantly related to abnormal weight. Physical inactivity was significantly related to lower education, longer working hours and lower income. CONCLUSION: This study showed that sociodemographic factors and stress were related with health-risk behavior with appropriate awareness in middle-aged people. These findings are expected to have implications for development of health promotion program focusing on stress management, improvement of sociocultural perception and simultaneous intervention for both smoking and alcohol. For middle-aged Korean women, more efforts must be made to remove economical and sociocultural barriers.
Drinking ; Education ; Female ; Health Behavior* ; Health Promotion ; Humans ; Male ; Middle Aged ; Smoke ; Smoking ; Telephone

The optimal management of lesions located in the brainstem(BS) is problematic. As an alternative to microsurgical resection, stereotactic radiosurgery employing the Gamma unit has been used to manage BS lesions, and this can provide relatively safe and effective management. This study describes our experience with 17 patients who underwent Gamma Knife radiosurgery(GKR) for vascular lesions of the brainstem between June 1989 and May 1996. Six of these had BS arteriovenous malformations(AVMs). The minimal radiation dose to the margin of AVMs ranged from 15 to 25Gy(mean, 18.9Gy). Four of six cases were partially obliterated, and on follow-up angiography, one small AVM was seen to be completely obliterated. Twelve months after GKR, one patient experienced a temporary neurologic deficit due to the effects of radiation and another patient, who had a large AVM, showed a permanent deficit as a direct result of treatment. There have been no instances of hemorrhage after GKR and all the patients are still alive. GKR was used to manage 11 patients with angiographically occult vascular malformations (AOVMs) of the BS. The periphery of the lesions received a radiosurgical dose of between 12 and 20Gy(mean, 15.5Gy). In four patients, the lesions became smaller, but in one, an increase was seen. In the remaining six, size change was not documented. One patient's neurological deficit worsened, though that might be related not to GKR but to non-fatal post-GKR rebleeding. At seven months, one patient developed a temporary neurologic deficit in association with perilesional edema that resolved over time. Three patients experienced post-GKR rebleeding, and none died during the follow-up period. We believe that GKR is an excellent option for patients with BS AVMs: when the risks of microsurgery are deemed too high, it is a course of action which seems reasonable. GKR does not, though, appear to obliterate AOVMs as effectively as it does AVMs. To assess the long-term effectiveness of the technique on these lesions, longer follow-up intervals will, however, be required.
Angiography ; Arteriovenous Malformations ; Brain Stem* ; Edema ; Follow-Up Studies ; Hemorrhage ; Humans ; Microsurgery ; Neurologic Manifestations ; Radiosurgery* ; Vascular Malformations

Tuberous sclerosis is a rare disease, which occurs sporadically or hereditarily and is recognized by its neurological and dermatological manifestations and may be accompanied with renal anomalies. The classical triad is composed of seizure, mental retardation and adenoma sebaceum on face. We experienced two cases of tuberous sclerosis in sporadic forms by mutation without any familial history which suggests the diseases were occurred by mutation rather than by autosomal dominant inheritance. In the first case, a 24-year-female patient with hypertension and abnormal renal function tests which were noted on the routine prenatal check at 32 weeks of gestation delivered normally at 37 weeks. The daughter of patient had seizure when she was 6 years old and was diagnosed as polycystic kidney disease by abdominal computed tomography. This case developed sporadic form of disease without familial history but, the daughter of patient might inherited by autosomal dominant form. The patient's clinical feature was characterized by history of epilepsy, painless abdominal mass due to polycystic kidney disease, abnormal renal function, skin abnormalites including angiofibroma and shagreen patch. Abdominal computed tomography demonstrated numerous variable sized cysts throughout both kidney. In second case, the patient was a 32-year-female patient complaining of 5kg weight gain, abdominal distension due to palpable masses. Her clinical feature was characterized by bilateral huge renal angiomyolipoma with normal renal function and skin abnormality such as erythematous papule on the face. Abd CT and MRI revealed huge angiomyolipoma of about 15cm X 18.5cm X 30cm and 14.5cm X 18cm X 30cm respectively. We presented the two cases with brief review of the literatures.
Angiofibroma ; Angiomyolipoma ; Child ; Epilepsy ; Humans ; Hypertension ; Intellectual Disability ; Kidney ; Magnetic Resonance Imaging ; Nuclear Family ; Polycystic Kidney Diseases ; Pregnancy ; Rare Diseases ; Seizures ; Skin ; Skin Abnormalities ; Tuberous Sclerosis* ; Weight Gain ; Wills

BACKGROUND: Many epidemiologic studies have reported on the controversial concept of the obesity paradox. The presence of acute kidney injury (AKI) can accelerate energy-consuming processes, particularly in patients requiring continuous renal replacement therapy (CRRT). Thus, we aimed to investigate whether obesity can provide a survival benefit in this highly catabolic condition. METHODS: We conducted an observational study in 212 patients who had undergone CRRT owing to various causes of AKI between 2010 and 2014. The study end point was defined as death that occurred within 30 days after the initiation of CRRT. RESULTS: Patients were categorized into three groups according to tertiles of body mass index (BMI). During ≥30 days after the initiation of CRRT, 39 patients (57.4%) in the highest tertile died, as compared with 58 patients (78.4%) in the lowest tertile (P = 0.02). In a multivariable analysis adjusted for cofounding factors, the highest tertile of BMI was significantly associated with a decreased risk of death (hazard ratio [HR], 0.57; 95% confidence interval [CI], 0.37–0.87; P = 0.01). This significant association remained unaltered for 60-day (HR, 0.64; 95% CI, 0.43–0.94; P = 0.03) and 90-day mortality (HR, 0.66; 95% CI, 0.44–0.97; P = 0.03). CONCLUSION: This study showed that a higher BMI confer a survival benefit over a lower BMI in AKI patients undergoing CRRT.
Acute Kidney Injury* ; Body Mass Index* ; Epidemiologic Studies ; Humans ; Mortality* ; Obesity ; Observational Study ; Renal Replacement Therapy*