Objective To explore the characteristics of brain activation when solving simple multiplication and complex multiplication tasks. Methods From June, 2010 to June, 2012, Thirteen normal subjects completed four functional magnetic resonance imaging (fMRI) ex-periments, including control tasks, visuospatial memory tasks, simple (single-digit) multiplication tasks and complex (multi-digit) multiplica-tion tasks. Results Compared with the control tasks, visuospatial memory tasks activated the bilateral occipital lobe, the right precuneus and superior parietal lobe;simple multiplication tasks activated the bilateral middle occipital gyri, the left superior parietal lobe, the left cingu-late gyrus, the left middle frontal gyrus and inferior frontal gyrus;complex multiplication tasks activated the right superior parietal lobe, the right inferior frontal gyrus, and the bilateral middle frontal gyri. Conclusion A right parieto-frontal network is involved in the multi-digit multiplication, which supports the containing of the spatial layout information.

The behavioral performance and mechanism of spatial acalculia were reviewed in this paper. Meanwhile, the brain mechanism and cognitive impairment are also preliminarily introduced.

Objective To explore the effects of computerized working memory training on working memory and brain functional activition for stroke paitents. Methods 3 stroke patients voluntarily joined in the study, 10 healthy adults were recruited as control group. The patients received computerized working memory training for 4 weeks. Cognitive psychological tests (the n-back task, Stroop task and Raven`s Advanced Progressive Matrices task) and neuroimaging test (task- state functional magnetic resonance imaging) were administered before and after treatment. Results Working memory training significantly improved the working memory, fluid intelligence and attention for each trained patient (P<0.001), and could renovate the abnormal functional activity model and reorganize the functional brain network. Conclusion Computerized working memory could be used as an effective cognitive rehabilitation intervention for stroke patients.

Objective To establish the reference values of static balance function with Active Balancer EAB-100 in healthy Chinese adults, and to screen the valuable indices for clinical work. Methods There were 227 healthy adults measured with Active Balancer EAB-100 when standing still with eyes opened and closed. All the data recorded were analyzed with SPSS 19.0, and the weight of every parameter was obtained with principal component analysis. Results The reference values of parameters for static balance function were established in healthy Chinese adults. The loadings of whole path length, rectangle area, circumference area, effective value area, deflection center displacement X, deflection center displacement Y, rectangle area of Romberg rate and circumference area of Romberg rate were more than average. Conclusion It is need to focus on whole path length, circumference area, deflection center displacement X, deflection center displacement Y, and effective value area and circumference area of Romberg rate much more in clinical practice for the static balance assessment.

Occupational Diseases ; prevention & control ; Occupational Health Services ; Public Health

Objective To investigate the toxicities and inhibitory effects of three kinds of kegin-type novel heteropoly compounds which were synthesised by molecular assembly interactions on influnenza A virus(H1N1) in vitro.Methods The toxicities and anti-influnenza virus activities were tested by methods of CPE-MTT and chick embryos culture.Results TC_(50) in MDCK cells and LD_(50) in chick embryos of three kinds of kegin-type novel heteropoly compounds were higher than those of amantadine.TI in MDCK cells and chick embryos were both higher than that of amantadine. Conclusion The three kinds of kegin-type novel heteropoly compounds have lower toxicities and higher anti-virus effects on influnenza A virus(H1N1) than amantadine.Through chemic modifications,the toxicity of amantadine can be decreased and the activity of amantadine can be increased.

Objective To investigate the clinicopathological features of tubular aggregate myopathy.Methods Eight patients as experimental group were diagnosed with tubular aggregate myopathy in Department of Neurology,People' s Liberation Army General Hospital,between March 2000 and March 2013.The data were retrospectively analyzed.Enzyme histochemical techniques and electron microscopy were taken to observe the muscle structures.Results The detection rate of tubular aggregate was 0.374％ (8/2 137).All of the 8 patients with tubular aggregate myopathy were male.Five patients presented with episodes of muscle weakness,while 3 patients presented chronic progressive muscle weakness.The main clinical features of all patients were muscle weakness.The creatine kinase level was mildly elevated in 4 patients,while it was normal in the other 4 patients.Electromyogram showed myogenic damage in 5 patients and normal in 3 patients.All of the 8 patients denied family history.By light microscopy,hematoxylin-eosin staining showed that tubular aggregates were multiple basophilic subsarcolemmal substance.Tubular aggregate stained red with modified Gomori trichrome stain,reacted intensely for nicotinamide adenine dinucleotidetetrazolium reductase,and remained unstained on oil red O,periodic acid Schiff,sudan black B,acid phosphatas,adenosine triphosphatase.By electron microscopy,tubular aggregates were densely packed tubules predominantly in the subsarcolemmal region.Conclusion Enzyme histochemical staining and electron microscopy show special features of tubular aggregate myopathy,are the critical techniques for the diagnosis of the disease.

It is first time to use dextren T-40 oxidative method to conjugate anti-gastric cancer mono-colonal antibody(McAb)with anti-tumor medicines of daunorubicin(DNR)and methotrexate(MTX)together.Cytotoxicity of conjugates was measured by MTT method and ~3H-TdR incor-poration method respectively.Both sensitivity is similar.The results have showed that this conju-gate exhibited selective cytotoxicity on human gastric cancer cells in vitro.

Objective To establish a method of gold nanoprobe-based solution hybridization (GNBSH) to detect nucleic acid sequence-based amplification (NASBA) products for the rapid diagnosis of invasive aspergillosis (IA).Methods The Aspergillus specific 18S rRNA was amplified by NASBA and then the amplified products were hybridized with the gold nanoprobes which were modified with thiol compounds at the 5'end.Serum samples from 106 patients,including 14 with a definite IA,32 with suspected IA and 60 without IA,were detected by the established method,and the obtained results were compared with that of galactomannan (GM) test to evaluate its accuracy.Results The gold nanoprobes only hybridized with Aspergillus NASBA products but not other non-Aspergillus strains.The sensitivity,specificity and the area under the ROC curve (AUCROC) of the established GNBSH method for detecting 106 clinical samples were 82.61％ (38/46),81.67％ (49/60) and 0.890,respectively.The sensitivity,specificity and AUCROC of GM test were 56.52％ (26/46),83.33％ (50/60) and 0.723,respectively.Conclusion The established GNBSH method to detect Aspergillus NASBA products has high sensitivity and specificity and simple operation,which may be used to detect the infection of Aspergillus by clinical laboratories.

Objective In order to reduce the toxicity and still maintain the bioactivity of the medicinal plant Tripterygium wilfordii(TW),the whole plant biotransfomed method was employed.By observing the pharmacological activities and the toxicity of MeOH extracts from the root of T.wilfordii that were biotransformed by the fungi Aspergillums spp.and Rhizopus spp.,the best biotransformed fungi strain has been selected based on the experimental data.Methods Applying the models of acute and chronic inflammation, the weight of immune organ and cell immunity were used to study the pharmacological activities,and ip injection to observe the acute toxicity.Results The anti-inflammation and immunosuppression have been increased slightly and the toxicity decreased significantly after being biotransformed by Aspergillus spp.(TW1),while the anti-inflammation,immunosuppression,and the toxicity have been declined after being biotransformed by Rhizopus spp.(TW2).Conclusion Although both selected fungi could change the pharmacological activities and toxicity of the T.wilfordii,Aspergillus spp.is better than Rhizopus spp.