Sporotrichsis is a chronic granulomatous mycosis caused by Sporotrichum species. In Korea, Kim et al. had reported two cases of cutaneous sporotrichosis involving upper extremities for the first time in 1960. They had determined their strain to be similar to a variant of S.beuermanni by culture. These Kim's strain has inoculated into the peritoneum of rats pathogenicity, virulence and course by examining the macroscopic The conclusion were as follows: 1. Rats were inoculated intraperitoneally and killed at intervals of 12, 20, 30, 40 and 50 days and followed by postmortem examination. Macroscopic findings: At twelve day and lesions were observed. At 20 to 50 days all showed grayish brown lymphnodules varying in size, which were disseminated on the mesentery, omentum, peritoneum and inguinal region. On lung, liver, spleen and kidney, various changes i. e.; congestion, necrosis and degenerative changes were noted at 30 to 40 days after inoculation. 3. Microscopic findings: a. Most nodules were characterized by three distinct zones, i. e.; central suppurative, mirldle tuberculoid, and peripheral syphiloid zones. b. Visceral organs showed the following pathological changes varying with the duration of infection; diffuse hepatic necrosis, bronchitis and pneumonitis, splenitis and degene- rative changes in the kidney. c. Microorganisms could found in tissue by PAS stain, Its frequency rate shows 52% in lymphnodules and 16% in visceral organs. 4. For some unknown reasons isolation of the organisms by culture was successful only from 3 rats.
Animals ; Autopsy ; Bronchitis ; Estrogens, Conjugated (USP) ; Kidney ; Korea ; Liver ; Lung ; Mesentery ; Necrosis ; Omentum ; Peritoneum ; Pneumonia ; Rats* ; Spleen ; Sporothrix* ; Sporotrichosis ; Upper Extremity ; Virulence

With the artificially applied local inflammatory stimulus by ultraviolet irradiation to the inner aspect of the left albino rat thigh skin, the tissue mast cell changes were observed for the nerve effectiveness on the irradiated thigh (Lt.) and non-irradiated opposite thigh (Rt.) and tongue. Using the distilled water (for control group), Bistrium Bromide blocking agent), Yhenobarbital Sodium (sedative and hypnotic) and (autonomic ganglion Reserpine (tranquillizer) were regularly subcutaneously injected every 8 hours, and the following results of most cell changes were observed. 1) Without ultraviolet ray irradiation, the rats of first group were received distilled water and average number of mast cells at the Rt. and Lt. inner aspects of thigh and tongue were gradually decreased and 24 hours after injection, the number of each part showed lowed curve, that was 85. 1%, 84. 8% and 91. 8% of normal average number. On the second and 4th day of the injection, increasing number of each part reached to the normal average number. The 7th day of injection maximum number of above average was l02.1%, 110.8% and 110.7% 2) With the ultraviolet ray irradiation the rats of second group received distilled water. At the Rt, and Lt. inner aspects of the thigh and tongue, the average number of mast cells gradually decreased and on the 4th day of the injection, the lowest number was 59.3,"o, 78.7% and 86. 2% of each average number. However on the 7th day the increasing number showed 75.0%, 84.6% and 89.6% of average number. 3) Group 3; Received Bistrium Bromide injection respectively, 24 hours after injection at the Rt. and Lt. inner aspects of the thigh the gradually decreasing number of mast cells showed the lowest curve, it accounted 70.6% and 74.0% of normal value. However at the tongue it was 77.2% on the second day of injection. Their highest number was seen on the 7th day, it was 95.7%, 100.5% and 107. 8% respectively 4) Group 4; Received Bistrium Bromide and ultraviolet ray irradiation, the average number of mast cells at the Rt. and Lt. inner aspects of the thigh and tongue showed decreasing curve. On the second day of injection the lowest number was 43.3%, 65.4% and 74.9/o of normal value respectively. On the 4th day of injection and irradiation the average number was increased, then after 7th day of injection maximum value was 93. 7%, 92.1% and 115.1% of normal number. 5) Group 5; Received Phenobarbital Sodium, the average number of mast cells at the Rt. and Lt. inner aspects of the thigh and tongue was gradually decreased, the lowest number was seen on the 24 hours after injection, it was 76.7%, 8.7% and 90. 9% of normal value. On the second and 4th day of injection, the curve was increased and on the 7tIi day, the highest value was 106.5%, 104.8% and 104. 6% of normal average value. 6) Group 7; Received Phenobarbital Sodium with ultraviolet rayirradiation at the Rt. and Lt. inner aspects of the thigh and tongue, the average number of must cells were gradually decreased. On the 4th day of injection and irradiation, they reached to the lowest number, and it was 43.3%, 80.6% and 74.0% of normal number. On the 7th day it showed the highest value of 74.9%, 97.2% and 95.8% respectively. 7) Group 7; Received Reserpine at the Rt. and Lt. inner aspect; of the thigh and tongue, the number of mast cells gradually decreased. On the 4 hours after injection 85.4%, 84.0% and 87.9% of average normal number was seen. On the second day, respectively number was increased. On the 7th day maximum increasing number was 103.1%, 115.2% and 95.0% of normal average number 8) Group 8; Received Reserpine and ultraviolet ray irradiation, the decreasing number of mast cells reached to the lowest value on the 4th day after injection, it was 53.5%, 80.3% and 84. 4% of normal value. On the 7th day of procedure, increased number showed 94.8%, 110.3% and 92.9 % of normal value. From the above experimental study, Ristrium Bromide, Phenobarhital Scdium and Reserpibne gave some effect to the change of mast cell at the irracliaticn area by ultraviolet ray and non-irradiated distant part.
Animals ; Ganglion Cysts ; Mast Cells* ; Phenobarbital ; Rats ; Reference Values ; Reserpine ; Skin ; Sodium ; Thigh ; Tongue ; Ultraviolet Rays ; Water

No abstract available.
Menisci, Tibial*

No abstract available.

Genotoxic agents can induce various DNA lesions. DNA-Protein Crosslinks(DPCs) were known as the important DNA lesions which could impair gene expression because DPCs had a high probability of resisting repair and persisting through cell cycle. This repair resistance of DPCs could have biological significance but had not been evaluated clearly yet. Most of the studies that have evaluated the repair of DPCs only compared the extent of DPCs repair with other DNA lesions. We injected K2CrO4, a genotoxic agent, into Sprague-Dawley rats intraperitoneally(5mg/kg) and isolated blood lymphocytes 12 hours later. These lymphocytes were cultured in the mitogen added growth media and mitogen free media separately. The degree of the repair of DPCs was monitored for 4 days by the K-SDS assay. 4 day later, the amount of DPCs decreased by 4.6% in the mitogen added media but in creased by 10.9% in the mitogen free media. These results showed that DPCs induced by K2CrO4 were not repaired easily and the DPCs were biologically significant DNA lesions. We thought the decrease of DPCs in the mitogen added media was not due to the repair of DPCs, but from the increase of normal cell proliferation. Therefore, it is very important to consider the proliferation of normal cells when estimating the repair of DPCs.
Animals ; Cell Cycle ; Cell Proliferation ; DNA ; Gene Expression ; Lymphocytes* ; Rats* ; Rats, Sprague-Dawley

Vitamin A and its metabolite, retinoic acid, have been recommended for the treatment of psoriasis, ichthyosis, acne vulgaris and Darier's disease which resulted from abaormal regulatory function of keratinocytes. In recent years retinoic acid analogs (retinoids) have been synthesized and shown to be more potent and less toxic than retinoic acid. Since these drugs are therapeutically effective for skin diseases, only with longterm adminiatration of large doses, their toxicities must be seriously considered. The liver is the main site for both storage and excretion of vitamin A and ita derivatives, which may produce injury to the liver and other organs as a result of excess of lysosomal enzyme control. Fatty vacuoles, infiltration of inflammatory cells, focal necrosis, increase in lysosomal enzymes, distension of the RER, decrease in the ribosomes, swelling of the mitochondria, and proliferation of the SER within the hepatocytes have been observed in livers of vitamin A- as well as retinoic acid-treated rats. Its excess may also cause mucous metaplasia of secretory cells of the pancreas. (countinued...)
Acne Vulgaris ; Amphotericin B* ; Animals ; Darier Disease ; Hepatocytes ; Ichthyosis ; Keratinocytes ; Liver ; Metaplasia ; Mitochondria ; Necrosis ; Pancreas ; Psoriasis ; Rats ; Ribosomes ; Skin Diseases ; Tretinoin ; Vacuoles ; Vitamin A ; Vitamins

No abstract available.
Diatomaceous Earth* ; Pneumoconiosis*

No abstract available.
Antibodies* ; Asian Continental Ancestry Group* ; Chiroptera* ; Encephalitis Virus, Japanese* ; Encephalitis, Japanese* ; Humans ; Korea*

No Abstract Available.
Hepacivirus* ; Hepatitis C* ; Hepatitis*

PURPOSE: This study was designed to evaluate usefulness of intravenous digital subtraction angiography (IV-DSA) in detection of peripheral arterial obstructive disease(PAOD) and in follow-up of percutaneous transluminal angioplasty(PTA). MATERIALS AND METHODS: 35 Patients who had clinical symptoms and signs of compromised lower extremity perfusion, was screened with IV-DSA and then performed confirmative conventional angiography. We obtained sensitivity, specificity and accuracy of IV-DSA by comparing the findings of IV-DSA with those of conventional angiography. 21 patients who had been performed successful PTA, were foliowed-up with IV-DSA in 3, 6, 12months. We studied patency rate and factors that influenced restenosis. RESULTS: The sensitivity, specificity and accuracy of IV-DSA were 100%, 97%, 97% in lilac artery, 92%, 96%, 93% in femoropopliteal artery, 85%, 75%, 70% in proximal tibioperoneal artery retrospectively. IV-DSA follow up after PTA showed patency rate of 67% in first 3month, 67% in 2nd 3month, 60% in next 6month. Longer length and more irregular surface of the stenotic site, and higher incidence of run-off of vessel and of residual stenosis in the patients with restenosis were noted. CONCLUSION: High sensitivity, specificity and accuracy of IV-DSA in evaluating PAOD suggest that IV-DSA is useful in screening, planning therapy and following up after PTA.
Angiography ; Angiography, Digital Subtraction ; Arterial Occlusive Diseases* ; Arteries ; Constriction, Pathologic ; Follow-Up Studies ; Humans ; Incidence ; Lower Extremity ; Mass Screening ; Perfusion ; Retrospective Studies ; Sensitivity and Specificity