The cytologic heterogeneity of the tumor cells in gastric signet ring cell carcinoma was studied with 13 surgically early gastric carcinoma specimens by means of histochemical stainings on mucin(periodic acid Schiff-alcian blue at pH 2.5, paradoxical concanavalin A, high iron diamine) and electron microscopy. Of the 13 cases of early gastric cell carcinomas, 6 were mucosal type and 7 were submucosal type. Eleven cases consisted of mixture of gastric and intestinal type signet ring cells and the remaining 2 of the mucosal type were entirely made of gastric type. The colonic goblet cell type was found in 4 of the submucosal type. Within the mucosa the tumor cells showed a layering phenomenon; type A signet ring cells were distributed at the central zone and type B and C at the superficial or deeper zone. Each type of signet ring cell showed variable mucin histochemical stainability of gastric and/or intestinal nature. Above finding strongly suggest that the variable phenotypes of signet ring cells result from a heterogeneity of cytoplasmic mucin as well as different stages of differentiation of signet ring carcinoma cells.

No abstract available.
Echocardiography* ; Echocardiography, Doppler, Color ; Ligation*

No abstract available.
Angiography ; Coronary Angiography* ; Coronary Vessels ; Echocardiography* ; Mucocutaneous Lymph Node Syndrome*

No abstract available.
Child ; Coronary Vessels* ; Echocardiography ; Humans

The double-chambered right ventricle is congenital or acquired cardiac anomaly, which is characterized by aberrent hypertrophied muscular bands that divide the right ventriclar cavity into two different pressure chamber. This anomaly can complicate the natural history of patient with isolated ventricular septal defect. We experienced two cases of cases of acquired DCRV, which confirmed by two separate cardiac catherterization and angiography. The purpose of this report is to show that the aberrant muscular bands may be nonobstructive in early infancy and that the obstructive effect is developed with time as the bands become progressively more hypertrophied.
Angiography ; Heart Septal Defects, Ventricular ; Heart Ventricles* ; Humans ; Natural History

No abstract available.
Child ; Humans

BACKGROUND: Exercise intensity may induce changes in inflammatory markers and adipocytokines. However, it is not clear whether acute exercise-induced change to adiponenctin is associated with inflammatory markers and other adipocytokines in obese middle-aged individuals. The aim of this study was to investigate the effects of acute aerobic exercise on circulating adipocytokines and inflammatory markers in obese middle-aged women. METHODS: Using a crossover design, 10 obese middle-aged women (aged 48.4+/-5.1 years; body mass index 29.6+/-2.9 kg/m2) exercised on the treadmill at different durations and velocities at 60% and 80% maximum oxygen consumption (VO2max) in order to equalize energy expenditure (400 kcal). Blood samples were drawn to measure glucose, insulin, fibrinogen, high-sensitive C-reactive protein, leptin, tumor necrosis factor-alpha (TNF-alpha), and adiponectin concentrations. RESULTS: Glucose, fibrinogen, and hsCRP increased significantly (P<0.05) after exercise whereas homeostasis model assessment-insulin resistance decreased significantly (P<0.05). Adiponectin, leptin, and TNF-alpha levels did not change significantly at immediate postexercise, and there were also no significant time x intensity interactions for all variables. The percentage change in adiponectin concentration from baseline to the end of exercise correlated with the percentage change in leptin concentration (r=-0.642, P<0.05) with 60% VO2max exercise. CONCLUSIONS: Our results indicated that, in obese middle-aged women, neither 60% nor 80% VO2max exercise alters adiponectin concentration, in spite of the changes to insulin resistance and inflammatory markers. However, after acute moderate exercise, adiponectin concentration may be associated with the change in leptin levels.
Adipokines ; Adiponectin ; Body Mass Index ; C-Reactive Protein ; Cross-Over Studies ; Energy Metabolism ; Exercise ; Female ; Fibrinogen ; Glucose ; Homeostasis ; Humans ; Inflammation ; Insulin ; Insulin Resistance ; Leptin ; Obesity ; Oxygen Consumption ; Tumor Necrosis Factor-alpha

In the course of immunoscreening of Clonorchis sinensis cDNA library, a cDNA CsRP12 containing a tandem repeat was isolated. The cDNA CsRP12 encodes two putative peptides of open reading frames (ORFs) 1 and 2 (CsRP12-1 and -2). The repetitive region is composed of 15 repeats of 10 amino acids. Of the two putative peptides, CsRP12-1 was proline-rich and found to have homologues in several organisms. Recombinant proteins of the putative peptides were bacterially produced and purified by an affinity chromatography. Recombinant CsRP12-1 protein was recognized by sera of clonorchiasis patients and experimental rabbits, but recombinant CsRP12-2 was not. One of the putative peptide, CsRP12-1, is designated CsPRA, proline-rich antigen of C. sinensis. Both the C-termini of CsRP12-1 and -2 were bacterially produced and analysed to show no antigenicity. Recombinant CsPRA protein showed high sensitivity and specificity. In experimental rabbits, IgG antibodies to CsPRA was produced between 4 and 8 weeks after the infection and decreased thereafter over one year. These results indicate that CsPRA is equivalent to a natural protein and a useful antigenic protein for serodiagnosis of human clonorchiasis.
Amino Acid Sequence ; Animals ; Antigens, Helminth/*genetics/isolation & purification ; Base Sequence ; *Cloning, Molecular ; Clonorchis sinensis/genetics/*immunology ; DNA, Helminth ; Gene Library ; Human ; Molecular Sequence Data ; Rabbits ; Recombinant Proteins ; *Repetitive Sequences, Nucleic Acid ; Support, Non-U.S. Gov't

We report a case of ciliated hepatic foregut cyst which was incidentally found in a 64 year-old man. The cyst, 6 cm in diameter, was unilocular, solitary and was located in the medial segment of left lobe, just below the Glisson's capsule. Microscopically, the cyst wall consisted of 4 layers; pseudostratified ciliated columnar epithelium, subepithelial loose connective tissue, smooth muscle bundles and an outermost fibrous capsule. Although cartilage or subepithelial sero-mucous glands were absent, the morphologic features of the cyst correspond with those of an incomplete form of brochogenic cyst.
Cysts

BACKGROUND: Up to now, sleep stages have traditionally been determined by the visual inspection of individual EEG waves. However, the exact physiological meaning of the sleep waves is not known. The purpose of this study was to try and find out the physiological parameters of the EEG of the sleep and wakefulness states by calculating one of the non-linear chaos parameter, the largest Lyapunov exponent (LLE), of EEG time series. METHODS: The digital EEG of the wakefulness with eye opening (WEO), wakefulness with eye closure (WEC), stage1 (S1), stage2 (S2), stage3 or 4 (S34) were recorded at centroparietal region (C4-P4 bipolar derivation) in 10 normal subjects. Lyapunov exponents of 50 EEG time series in different states were compared. RESULTS: LLE's of WEO, WEC, S1, S2, S34 showed an increas-ing tendency as states switched from wakefulness to sleep. LLE of sleep was larger than that of awake state. CONCLUSIONS: The EEG of the sleep state appeared to be more chaotic than that of the awake state. This nonlinear chaos parameter can be used as a physiological parameter of normal sleep and awake states.
Electroencephalography* ; Sleep Stages ; Wakefulness*