Objective To observe the effect of local curettage combined with ointment in fistula applying in refractory apical fistula.Methods 56 cases of patients with refractory apical fistula one month after faultless root cannal therapy divided into group A and B by lottery,with 28 cases in each group.Local curettage combined with ointment in fistula applying in group A,and no processing applying in group B.One year fellow up was given to every patient to compare the effect between the two groups.Results All the 28 cases of patients with refractory apical fistu-la were recovering in three months.The improvement rate was 100%,recovery rate 100%,and the mean time of fistula disappearing was (0.5 ±0.1)months,time of shadow around root tip disappearing (2.5 ±0.7)months.Four cases in group B were improved in three months,three cases in six months and one case in nine months,with two cases recove-ring.The improvement rate was 28.57%,recovery rate was 7.14%,and the mean time of fistula disappearing was (5.0 ±0.7)months,time of shadow around root tip disappearing (9.4 ±1.0)months.The improvement rate in group A was higher than that in group B (χ2 =33.749,P<0.05),recovery rate in group A was higher than that in group B (χ2 =49.727,P<0.05).The mean time of fistula disappearing in group A was shorter than that in group B (t=3.081,P<0.05),mean time of shadow around root tip disappearing in group A was shorter than that in group B (t=5.042,P<0.05).Conclusion It was effective to apply local curettage combined with ointment in fistula applying in refractory apical fistula,and it is worthy of clinical popularization.

Emodin is the main effective monomer of Rhubarb, which has a variety of pharmacological actions and valuable clinical applications.Recently, there are many reports about molecular mechanisms of emodin pharmacological actions,and they givehighly attention on the dephlogisticating and antineoplastic effects. These effects are achieved through affecting the concentration and transportation of ions, resisting of oxidation and free radical, and affecting the secretion of inflammatory factor and the enzyme activity and apoptosis and so on. This article summarizes the reports and hopes to provide theoretical basis for the practical application.

Histone deacetylases (HDACs) catalyze the deacetylation of histone and nonhistone proteins. They play important roles in regulating chromatin remodeling and gene transcription,and that aberrant chromatin remodeling and transcriptional dysregulation are associated with many neurodegenerative diseases. Preclinical studies have suggested that histone deacetylases inhibitors (HDACIs) can target diverse pathophysiologies of ischemic stroke,not only reduce neuronal damage and infarct volume,but also promote the neuronal plasticity and functional recovery after ischemia. This article mainly reviews the neuroprotective mechanisms of HDACIs in ischemic stroke.

No abstract available.
Adenomatous Polyposis Coli*

Objective:To detect the influence of the early Class Ⅲ orthopedic treatment on the development of the maxillary.Methods:Four adolescence rhesus monkeys were fitted with casting Twin-block magnetic appliance(TMA),two were put to death after 1.5 months and another two after 3 months,zygomaticofrontal sutures were harvested,and performed histological examination using optical and electron microscope.Another two monkeys without appliance were served as control.Results:The maxillary sutures were rebuilt,after Class Ⅲ functional orthopedic,and the zygomaticomaxillary suture,zygomaticotemporal suture and transverse palatine suture were especially active.Electron microscopy results showed that osteoblasts and fibroblasts were active,and fibroblasts showed more active effects.Conclusion:During the treatment of the Class III functional orthopedics,maxillary sutures take part in the rebuilding actively,and there are lots of new bone formation along the sutures.

Objective To understand the hygienic situation of drinking water in the rural areas in the river system of the Yangtze and Huaihe in Jiangsu Province,and to provide the scientific evidence for improving the quality of drinking water. Methods From January to October in 2007,according to the guideline of drinking water quality monitoring in the rural areas,in the river system of the Yangtze and Huaihe in Jiangsu Province,in 53 counties (cities),the drinking water quality of the central water supply and the non-central water supply was investigated. Results From the result of comprehensive evaluation,the eligible rate of drinking water in the rural areas in the river system of the Yangtze and Huaihe in Jiangsu Province was 41.34%. As for residual chlorine the eligible rate was 49.64%,as for total coliform group it was 79.69%,as for total number of bacteria it was 88.78%,as for the turbidity it was 85.75%,as for fluoride it was 92.63%,as for the other indexes it was above 95%. Compared with the Huaihe river system,the fluoride content in the Yangtze river system was lower,total coliform group,turbidity and manganese content were higher (P

Objective: To observe the clinical effect of midnight-noon ebb-flow day-prescription of acupoints on biliary colic. Methods: 60 cases with biliary colic were randomized into two groups, 30 cases in each group. Day-prescription of five transport points was employed in the treatment group, while point selection based on pattern identification was employed in the control group. Results: The pain-killing rates between the two groups had no statistical differences (P>0.05). Conclusion: Day-prescription of acupoints can obtain similar pain-killing effect with point selection based on pattern identification, but with less points and treatments.

Aim: To establish a method for the determination of pantoprazole sodium and its related substances. Methods: A column packed with octadecylsiance bonded silica gel (250 mm × 4. 6 mm, 5 pjn) was used. The 0. 01 mol/L monopotassium phosphate solution( adjusted with phosphoric acid to pH 7. 0) -acetonitrile were adopted as the mobile phase, a gradient elution was programmed as follows: 0→30 min(90:10-60:40), 30→45 min(60: 40→15: 85); the detection wavelength was 289 nm; the column temperature was 40 ℃; the flow rate was 1. 0 mL/min. Results: Pantoprazole sodium, the intermediates and its related substances could be well separated. A good linear relationship was obtained over the range of 6. 96-48. 72 μg/mL( r =0. 999 9). The limit detection and quantisation of pantoprazole sodium were 8.51 ng and 17.0 ng, respectively. Conclusion: This method can be applied to control the related substances of pantoprazole sodium and determine pantoprazole sodi-um.

Objective To study the effects of overexpression of HRAD17 on radiation-induced apoptosis of A549 cells.Methods Transfection of FLAC-tagged HRAD17 expression plasmid into human A549 cells was carried out and flow cytometry was employed tO observe the apoptotic effect.In addition,phosphorylation of p53 Ser46 was detected by Westem blot and the transcription of p53 AIP1 was measured by RT-PCR.Results Overexpression of HRAD17 gene significantly increased radiation-induced apoptosis of A549 cells.Further.Ser46 phosphorylation of P53 protein was increased and transcription of p53 AIP1 gene was elevated in cells overexpressing HRAD17.Conclusions HRAD17 protein can sensitize A549 cells to radiation-induced apoptosis in part by increasing p53 AIP1 expression.

Objective To investigate effect of platelet-associated antibody to the platelet transfusion.Methods Platelet-associated antibody in 120 patients who needed repeated platelet transfusion for long-time was determined by enzyme-linked immunosorbent assay (ELISA),and platelet count was assayed before and after blood transfusion,and the effect of platelet transfusion was investigated by corrected platelet count.Crossmatch approcah was used to screen compatible platelet for patients with platelet relative antibody positive.Results The ratio of positive platelet-aasociated antibody in patients who needed repeated platelet transfusion was 65.0％,and the ratio of platelet transfusion resistance was 61.7％.The ratios of platelet transfusion resistance were 84.6％ and 19.0％ respectively in patients with positive platelet-associated antibody and in patients with negative platelet-associated antibody,which were different in statistic in both groups( x2 =,46.914,P ＜0.0l ).The effective ratio of platelet transfusion in patients with positive platelet-associated antibody after crossmatch approach was 85.9％.Conclusion Positive platelet-associated antibody was related to platelet transfusion resistance with high risk,and crossmatch approach could increase the effect of platelet transfusion.