Objective To investigate the effect of steroid hormones on lymphatic stomata of ovarian bursa and fluid adsorption. Methods The lymphatic adsorption was traced by trypan blue,and the ultrastructure of the lymphatic stomata and estrogen receptor level of mesothelial cells on ovarian bursa was observed by scanning electron microscopy,transmission electron microscopy and immuno-colloid golden technology. Results The absorption and opening area of lymphatic stomata were enhanced in estrogen group mice,and decreased in androgen group as compared to control group mice.Estrogen receptor was first identified in the nuclei of mesothelial cells on the inner layer of mice ovarian bursa and the level was down-regulated by estrogen and slightly up-regulated by androgen.Conclusion The absorption and the opening area of lymphatic stomata can be regulated by steroid hormones in mice,and the machanism may be related to the expression levels of estrogen receptor in the nuclei of mesothelial cells.

Emodin is the main effective monomer of Rhubarb, which has a variety of pharmacological actions and valuable clinical applications.Recently, there are many reports about molecular mechanisms of emodin pharmacological actions,and they givehighly attention on the dephlogisticating and antineoplastic effects. These effects are achieved through affecting the concentration and transportation of ions, resisting of oxidation and free radical, and affecting the secretion of inflammatory factor and the enzyme activity and apoptosis and so on. This article summarizes the reports and hopes to provide theoretical basis for the practical application.

Neuropathic pain is the clinically common disease and does great harm to physical and mental health.Because its pathogenesis is still unclear and there aren′t effective therapeutic tools and the course of chronic pain lasts a long time,the study of neuropathic pain has become the hot spot and the focal point of pain research area.This article reviews various kinds of animal models of neuropathic pain,which can repeat neuropathic pain symptoms of mankind and may be effective ways to make research.By acting on P2X receptors,ATP is the signal of an important transmitting pain.By utilizing animal models of neuropathic pain,it shows that P2X receptors have an important role in the formation,transmission and regulation of pain and may be a new target of neuropathic pain therapy.

OBJECTIVE:To Study the cost-effectiveness among3therapeutical schemes in the treatment of Graves ophthalmopathy.METHODS:A total of94cases with Graves ophthalmopathy were randomized into3groups,Group A was assigned to receive medrat,Group B to receive dexamethasone and Group C to receive prednisone,the outcomes were evaluated in terms of the cost-effectiveness analysis.RESULTS:The costs for Group A,B and C were2956.76yuan,2059.42yuan and31.09yuan,respectively.The total effective rates were93.94%,93.75%and51.72%,respectively.The cost-effec-tiveness ratios were31.47yuan,21.97yuan and0.60yuan,respectively.The incremental cost-effectiveness ratios of Group A and B were69.30yuan and48.26yuan,respectively as against Group C.CONCLUSION:In terms of pharmacoeconomics,Group B is comparatively the best option in treating Graves ophthalmopathy.

Objective To discuss the application effect of TCM constitution recognition on TCM chronic disease refining health management for type 2 diabetes mellitus.Methods Totally 140 outpatients with type 2 diabetes mellitus from Chaoyangmen Community Health Center were selected and divided evenly into two groups after TCM constitution assessment. The study lasted for 2 years (1st August 2013–31st July 2015). The control group (70 cases) received diabetes community health management. In addition to routine community health management, TCM constitutional management was also administrated in the intervention group. The latter group received medicine, food, sport guidance according to the results of TCM constitution assessment. The blood glucose, blood lipid, BMI and waist-hip ratio of the two groups were monitored, and the self-management and life quality of patients were evaluated. Results After the intervention, significant improvement was found for fasting blood glucose, 2 h postprandial blood glucose, BMI, waist-hip ratio and blood lipid both in the control group and the intervention group, and the improvement in the intervention group was more obvious than the control group (P<0.05). The whole level of self-management and life quality was greatly increased in both groups, while improvement of the intervention group was much higher than the control group (P<0.05).Conclusion Integrated application of TCM constitutional theory in TCM chronic disease refining health management for type 2 diabetes mellitus will contribute to improving patients’ self-management, changing their clinical symptoms and finally improving their life quality.

ObjectiveTo investigate the effects of alpha-lipoic acid on serum soluble intercellular adhesion molecule-1 (sICAM-1) and high sensitive-C reactive protein (hs-CRP) levels in patients with early diabetic nephropathy (DN).MethodsA total of 61 patients with early DN were randomized into treatment group (n =31 ) and control group ( n =30 ).The two groups were both treated with dietary control and oral hypoglycemic drugs or insulin.Meanwhile,the treatment group was additionally given alpha-lipoic acid 300 mg/d by intravenous infusion for 20 days.The levels of sICAM-1 and hs-CRP in each group were detected.Results The levels of sICAM-1 and hs-CRP were decreased significantly in alpha-lipoic acid treatment group after 20 days of therapy ( [ 198.03 ±23.67] μg/L vs [271.17 ±34.66] μg/L,[5.16 ±0.43] mg/Lvs [7.95 ±0.88]mg/L,P ＜0.01 ).Conclusion alpha-lipoic acid may decrease the expression of slCAM-1 and hs-CRP,and that may be one of the mechanisms to postpone the progress of early DN.

Objective To explore the relationship of coping style and social support with hope in maintenance hemodialysis patients.Methods A total of 177 maintenance hemodialysis patients were recruited from 3 dialysis centers in Guangzhou and were investigated with the self-designed questionnaire,the Herth Hope Scale,Simplified Coping Scale and Social Support Scale.Structural equation model was used to establish the model of relationship of coping style and social support with hope level.Results The average score of hope in maintenance hemodialysis patients was (31.83±3.69) points.The total score and all the sub-scores of social support were (42.24±4.09) points,(23.72±2.36) points,(10.82±2.26) points,(7.70±1.36) points respectively.And the average scores of positive coping style and the negative coping style were (2.15±0.28) points,(1.56±0.33) points,respectively.Social support had direct positive effect on hope level,and the positive and neg-ative coping style had indirect positive and negative effect on hope level respectively.Conclusions Social support has a direct effects on hope level in maintenance hemodialysis patients.Positive and negative cop-ing styles have indirect effects on hope level through social support.

Cytokine induced killer (CIK) cells are the main immune cells for tumor immnuotherapy.Relevant basic researches show that CIK cells have significant antitumor effects for various hematologic tumor and solid tumors.Clinical studies confirm that CIK cells-based therapy have exact therapeutic effect and mild adverse reaction for tumor patients.So CIK cells-based therapy is a promising and safe method and it is important to establish standards for cultivating and using CIK cells.

Objective To prepare the long‐term use and good stability of HBsAg feeble masculine serum ,and the application of controlling quality for HBsAg .Methods The HBsAg positive serum was diluted using calf serum phosphate buffer by grads ,multi‐ple to 1∶2 ,1∶4 ,1∶8 ,1∶16 ,1∶32;HBsAg feeble masculine serum was detected by double antibody sandwich method of enzyme‐linked immunosorbent assay (ELISA) using different reagent lot obtained from two companies for continuous six months .Results The serum ,s diluting multiple which the S/CO ,value between 1 to 3 was 1∶8 ,and the S/CO was 2 .42 ,the CV was 4 .72% .The serum of quality control possessed a good stability in different detection reagents ,the S/CO value range from 2 .41 to 2 .82 ,and the CV value was 4 .24% to 8 .68% .The CV value of HBsAg feeble masculine control serum with 6 months successive detection ranged from 8 .71% to 9 .86% (CV<10% ) .Conclusion The HBsAg feeble masculine serum possessed good stability and precision which is fit for masculine serum in HBsAg test .

Cerebral small vessel disease (CSVD) refers to the syndromes of clinical,cognitive,radiographic and pathological manifestations caused by intracranial small vessel disease.Due to the insidious onset of CSVD,unknown etiology,diverse clinical manifestations,and better short-term prognosis,it is easily ignored,resulting in misdiagnosis,missed diagnosis,and non-standard diagnosis and treatment.This article reviews the advances in research on clinical manifestations,imaging features,diagnosis and treatment of CSVD.