Cholangiocarcinoma is a type of malignant tumor with high destruction.Due to its low diagnostic rate and high fatality rate,the operation is the unique therapeutic methods for the radical cure.However,the diagnosis and treatment for the disease were always in the phase of progression,so currently,the radical therapeutic rate is quite low,while the recurrence rate of the operation is extremely high.If the correlated mechanism of perineural invasion of cholangiocarcinoma could be understood,then interrupted its perineural invasion in the early period,that could greatly enhance the prognosis of cholangiocarcinoma patients.This article systematically reviews the progress of cholangiocarcinoma neural invasion related molecules and possible mechanism.

Atherosclerosis ; complications ; pathology ; Humans ; Thrombosis ; complications ; pathology

OBJECTIVE:To try to establish a combined serum-pharmacology and electrophysiology method for the study of mechanism of chuanxiong extract in dilatating coronary artery.METHODS:Chuanxiong extract was intragastrically given to rats and mice,then the serum contents of tetramethylpyrazine(TMP)were determined by HPLC,which provided data for electrophysiological study.The action of chuanxiong extract on Ca 2+ -activated K channel(Kca)of smooth muscle of coronary artery in pigs was studied by patch clamp technique.RESULTS:The calibration curve of TMP was linear in the range of0.216～54?g/ml(r=0.9999).The recovery was100.7%～102.4%.The serum concentrations of TMP in rats and mice were(3.56?3.01)and(8.19?6.57)?g/ml respectively.The chuanxiong extract could not markedly activate the Kca of smooth muscle of coronary artery in patch clamp study.CONCLUSION:The established method for TMP determination is feasible and relieable.In order to improve the combined electrophysiology and serum pharmacology technique,high quality medical materials should be adopted and the bioavailability improved.

OBJECTIVE:To screen the effective antioxidant in tretinion ointment METHODS:The six tretinion ointments were prepared with different antioxidants and the same base Based on the accelerated test design,the tretinion concentration was determined by HPLC RESULTS:The ointment with the antioxidant D exhibited the least oxidation rate CONCLUSION:Data from accelerated test demonstrated that the compound antioxidant D was the best one in this formula

OBJECTIVE: To study the dissolution amounts of aciclovir (ACV) from differently based preparations and provide the evidence for the development of new preparation of ACV. METHOD: The dissolution tester was applied for the quantitative determination of ACV released from 3 based formulations and 5 concentrations samples. The release amount per unit area (M) and the accumulative release percentage (Q) were compared respectively. RESULTS: The value for M was raised with the increase of the concentration of aciclovir in preparations, and for Q was reduced. The release rates of the aciclovir gel were at least two times faster than the creams and the ointment. CONCLUSION: The drug dissolution from ACV gel was better than the creams and the ointment. 10 gkg-1 ACV in prearation was the suitable concentration.

Objective To evaluate the effect of direct stenting on no-reflow in patients with ST-segment elevation (STEMI) acute myocardial infarction. Methods A total of 157 patients with STEMI received direct stenting (DS,n=85) or conventional post-dilation stenting (CS,n=72). The time of X-ray exposure, the amount of contrast media consumed, the TIMI grading after stenting and ST-segment changes of electrocardiogram were compared between two groups. Results The time of X-ray exposure and the amount of contrast media consumed were significantly less in DS group than those in CS group [(24.6?16.9) min vs (34.4?17.5) min, (115?37) mL vs (166?61) mL, P

Objective To investigate the significance of symptom-to-ballon time on patients with ST elevation acute myocardial infarction (STEMI) treated with primary percutaneous coronary intervention (PCI). Methods 171 patients, in which ANGIOGUARD XP distal protection devices were applied in 18 patients, were divided into three groups according to their symptom-to-ballon time: group A (

Objective To study the clinical effect of SCOFIX on thoracolumbar fracture.Methods The clinical data of twenty-five cases with thoracolumbar fracture treated with SCOFIX system were retrospectively anal- ysed.Results With six to twenty-two months follow-up,anterior height of compressed vertebral bodies was restored from 46.8 %(12%～60%)preoperatively to 92%(90%～100%)postoperatively,and that of posterior height was from 77.5%(26%～86%)to 96.7%(90%～100%).Cobb's angle was from 15.7?(12?～26?)preoperatively to 1.4?(0?～6?)postoperatively.Conclusion SCOFIX system was simple structured,easily used and strongly fixed, and it was a good system in spinal internal fixation and for deeply reseach.

We wished to sequence the full-length genomes of the DHL10M110 strain of the Akabane virus (AKV) isolated from mosquitoes in Yunnan Province, China, in 2010. We also wished to analyze the characteristics of these complete nucleotide sequences. The complete genomic sequence of the DHL10M110 strain from Yunnan Province was obtained by reverse transcription-polymerase chain reaction and direct sequencing. We found that the length of the L, M and S gene nucleotide sequences of the DHL10M110 strain were 6 869-bp, 4 309-bp and 856-bp, respectively, including the open reading frame (ORF) nucleotide sequences of 6 756-bp (L), 4 206-bp (M) and 702-bp (S), encoding 2252, 1402 and 234 amino-acid polyproteins, respectively. Phylogenetic analyses based on L-fragment ORF showed that the DHL10M110 strain had a close relationship with the OBE-1 strain of the AKV from Japan and AKVS-7/SKR/2010 strain of the AKV from South Korea. Phylogenetic analyses based on M- and S-fragment ORF showed that the DHL10M110 strain had a close relationship with the epidemic strains of the AKV from Japan, South Korea and Taiwan, but that the DHL10M110 strain had a lone evolutionary branch. In terms of nucleotide (amino acid) homology, the similarity of L-, M- and S-fragment ORFs of the DHL10M110 strain to the OBE-1 strain from Japan was 92.6% (98%), 88.5% (94%) and 96.4% (99.1%), respectively. When comparing the DHL10M110 strain with the OBE-1 strain, we noted 45, 84, and 2 different sites in the amino acids of L, M and S fragments, respectively. Homology and phylogenetic analyses also suggested that the DHL10M110 strain had a distant relationship with the epidemic strains of the AKV from Kenya and Australia. Also, we confirmed by complete genomic sequence analyses that the DHL10M110 strain was clade-Asia of the AKV. However, differences between the DHL10M110 strain compared with strains from Japan and South Korea were also noted. These results suggest that the DHL10M110 strain harbored relatively stable genetic characteristics and distinct regional features. This is the first time that full-length genomic sequences of the DHL10M110 strain of the AKV in mainland China have been obtained.
Amino Acid Sequence ; Animals ; Base Sequence ; Bunyaviridae Infections ; transmission ; virology ; China ; Culicidae ; virology ; Female ; Genome, Viral ; Humans ; Insect Vectors ; virology ; Male ; Molecular Sequence Data ; Open Reading Frames ; Orthobunyavirus ; classification ; genetics ; isolation & purification ; Phylogeny ; Sequence Alignment ; Viral Proteins ; chemistry ; genetics