2.Radiographic Contrast-Induced Nephropathy in 6 Children
Journal of Applied Clinical Pediatrics 2006;0(17):-
Objective To investigate the clinical characteristics of radiographic contrast-induced nephropathy(RCIN) in children for improving the knowledge of this disease.Method The clinical data of 6 hospitalized children with RCIN collected from Oct.1998 to Dec.2007 were evaluated retrospectively.Results Of 92 patients who had cardio-angiography,intravenous pyelography,renal arteriography,cerebral angiography,and CT with contrast medium,6 cases(2 girls and 4 boys,aged 2-17 years old) had RCIN.Among the 6 children,5 cases [serum creatinine(Scr) was from 168.3 to 249.7 ?mol/L] showed non-oliguria-type acute renal failure;1 case(Scr was 583.1 ?mol/L) showed oliguria-type acute renal failure,and renal biopsy pathology findings showed that renal tubular epithelial cells were vacuolar degene-ration,striated border fell off,renal tubular epithelial cells were necrotic mulifocally,while the glomeruli were normal.Hematuria in 2 cases with primary disease aggravated;2 cases showed microscopic hematuria;1 case had proteinuria(+),1 case appeared microscopic hematuria and proteinuria(+).Five patients with non-oliguria-type acute renal failure were cured successfully after the medication of 10-21 days.Another case with oliguria-type acute renal failure was well after interrupted hematodialysis for 13 days and medication.Conclusion Children suffering RCIN usually manifest non-oliguria-type acute renal failure,and most of them can be cured by hematodialysis and medication mostly.
4.Establishment of restriction landmark genome scanning for detection of genomic DNA methylation of tissues
Yun QIN ; Da-Jun DENG ; Li YU ;
Chinese Journal of Laboratory Medicine 2003;0(09):-
50 000 bp)was extracted from frozen gastric cancer tissue and their corresponding normal samples and used for RLGS assay.The genome DNA was digested by methylation-sensitive restriction enzyme Not Ⅰ, and labeled by radioisotope ~(32)p,then separated by two-dimensional electrophoresis and autoradiography. Experimental conditions for each step were optimized step by step.DNA fragment sequences for the dots on scanning profile were identified according to the human RLGS database.Results RLGS assay was set up and used to get the RLGS profiles of the representative tested samples successfully.These profiles can display more than 1 200 available dots averagely,the profiles of high quality DNA sample can display more than 1 800 dots which is the average level at an excellent RLGS lab,discrepant dots which had weaken or enhanced signals and their sequence information were obtained.The result can be reproduced.Conclusion The RLGS assay is established,stabilized for detection of DNA methylation of tissue samples.
5.Quality classification standard of Scrophularia ningpoensis seedlings.
Xue ZHANG ; Da-xia CHEN ; Jun TAN ; Long-yun LI
China Journal of Chinese Materia Medica 2015;40(6):1079-1085
The morphological indexes of the Scuophularia ningpoensis seedlings including the longth, diameter and weight were measured, clustering analysis was used to set up the standard quality grading of seedlings of S. Ningpoensis by SPSS. Field experiment was carried out to measure the indicators of plants growth and development, the yield and the quality. The results showed that the growth and yield of class I seedlings were better than those of class II and III. The content of main active ingredients was affected barely by seedlings classification. To ensure the quality, class II seedlings or above should be used for plantation. The established quality classification standard of S. ningpoensis seedlings was scientific and feasible, and provides the basis for the standardized cultivation of S. ningpoensis.
Drugs, Chinese Herbal
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analysis
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standards
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Quality Control
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Scrophularia
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chemistry
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classification
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growth & development
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Seedlings
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chemistry
;
growth & development
6.Successful treatment of a child with respiratory failure occurred after lime inhalation.
Jie WANG ; Da-bo LIU ; Zhen-yun HUANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2008;43(10):792-793
Calcium Compounds
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Foreign Bodies
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complications
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etiology
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Humans
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Infant
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Male
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Oxides
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Respiratory Insufficiency
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etiology
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therapy
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Treatment Outcome
7.Diagnosis and treatment of acquired idiopathic laryngomalacia.
Da-zhang YANG ; Jun HAN ; Yun FENG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(6):468-469
Adult
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Aged
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Cartilage Diseases
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diagnosis
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therapy
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Humans
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Laryngeal Cartilages
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Laryngeal Diseases
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diagnosis
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therapy
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Male
;
Middle Aged
8.Eleven cases of drug-induced arsenic poisoning.
Da-wei LIN ; Yan-jie PENG ; Chen-yun YANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(3):227-227
Adult
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Aged
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Arsenic Poisoning
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drug therapy
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etiology
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Drugs, Chinese Herbal
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adverse effects
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Female
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Humans
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Male
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Middle Aged
9.Research and development of a clinical laboratory information system.
Da-gan YANG ; Gen-yun XU ; Yang-jun ZHU
Chinese Journal of Medical Instrumentation 2005;29(1):23-26
A clinical laboratory information system consists of two parts--the information system and the management system. Its development is based on scientific and rational lab-workflow, consulting the international standard HL7 Protocol, and combined with barcode technique and instrument communication. The information system mainly manages the data which come from the whole lab testing process while the management system is dominating the lab office work and management decisions.
Automatic Data Processing
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Clinical Laboratory Information Systems
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standards
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Computer Communication Networks
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Databases as Topic
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Management Information Systems
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Software Design