No abstract available.
Silver*

Nucleic acid sequence-based amplification(NASBA) is a sensitive,isothermal,transcription-based amplification system specifically designed for the detection of RNA targets,which could amplify templete RNA in 2h under isothermal condition at about 42?C and without any special equipment.NASBA is now widely applicated in diagnosis of many pathogenic microorganism.It is mainly about principles and applications of NASBA in viral diagnosis.

No abstract available.
Cytomegalovirus Infections*

A 12-year-old boy showed a slatecolored scaly patch on h; ight face for 2 months. Histopathologic findings showed basal vacuolations and dermal me arphages with only scanty lymphoid cells in the fat tissue layer. Besides the facial lesion, lesions of edematous localized erythema were noted on his right if and left buttock with similar mi rocopical findings. 6 months later the facial lesion was aggravated like a cellulitis, of which thc bevpsy findings were consistent with mycosis fungoides. The patient received chemotherapy aftei the diagonsis for one month before his termination. Because of the rarity of mycosis fungoides in childhood age and its un common cutaneous lesions, the case is reported.
Buttocks ; Cellulitis ; Child ; Dronabinol ; Drug Therapy ; Erythema ; Humans ; Lymphocytes ; Male ; Mycosis Fungoides* ; United Nations

The Baboon syndrome is used to denote a characteristic distribution pattern of systernic allergic contact dermatitis. Diffuse erythema of the buttock, upper inner surface of the thighs and axillae are characteristic features. We report three cases of Eaboon syndrome developed after contact with mercury through breaking a clinical thermometer in a 30 year-old, 16 year-old, 32 year-old females, respectively. Mercury sensitivity was confirmed by a patch test in all three patients.
Adolescent ; Adult ; Axilla ; Buttocks ; Dermatitis, Allergic Contact ; Erythema ; Female ; Humans ; Papio* ; Patch Tests ; Thermometers ; Thigh

No abstract available.
Urticaria Pigmentosa* ; Urticaria*

BACKGROUND: Skin cancers arc the most common malignancies in human. The increasing incidence of skin cancer has been attributed to increasing exposure of the papulation to sunlight and other factors, such as chemicals or. viruses. OBJECTIVE: The purpose of this study is to investigate a recent 10 year period(1984-1993) of clinical observation of cutaneous malignant tumors. METHODS: A statistical study was made with 60 cases of malignant tumors that visited the Departrnent of Dermatology, Chung Ang University during a 10 year period(1984-1993). RESULTS: 1. The mean incidence of cutlneous malignant tumors in the total number of outpatients was 0.16%(60/36,416). The incidence of malignant turnors has tended to increase in the last 4 years. 2. The age of onset was quite varied but the majority of cases, 51 cases(85.0%), were over 40 years old. The ratio of rnen to women was 1.3: 1.3. Among the 60 cases of malignant cutaneous tumors, the most frequently observed tumor was basal cell carcinoma, 20 cases(33.3% ). The next most frequent tumors were squamous cell carcinoma, 9 cases(15.0%), metastatic carcinoma, 9 cases(15.0%) and malignant melanoma, 6 cases(10.0%). 4. Among the 20 cases of basal cell carcinoma, 19 cases(95.0%) were over 40 years old. The most frequent age was the 7th decade(30.0%). The most common predilection sites were the nose (30.0%), the cheek(20.0%) and the eyelid(10.0%). 5. Among the 9 cases of squamous cell carcinoma, 8 cases(88.9% ) were over 40 years old. The ratio of men to women was 1: 2. The most frequent age was the 8th decade(44.4% ). The most common predilection sites were the head(55.6%), lower extremity(22.2%), upper extremity (11.1%) and trunk(11.1%). 6. All patients with malignant melanoma were over 40 years old. The most frequent age was the 5th decade(50.0%). The most cornmon predilection sites were the upper and lower extremities (66.7% ). 7. Among the 9 cases of metastatic carcinoma, 7 cases(77.8%) were over 40 years old. The ratio of men to women was 1: 1.25. The most frequent primary tumor was stomach cancer in men and lung cancer in women. 8. Cutaneous malignant tumor. usually had no detectable metastasis but there were 2 cases of extramammary Pagets disease with metastasis in the regional lymph nodes. CONCLUSIONS : This study revealed that the incidence of malignant tumors has tended to increase over the last few years, the most frequent age was over 40 years old, the most observed turnor was basal cell carcinoma and any type of cutaneous malignant tumors was rarely metastasis.
Adult ; Age of Onset ; Carcinoma, Basal Cell ; Carcinoma, Squamous Cell ; Dermatology ; Female ; Humans ; Incidence ; Lower Extremity ; Lung Neoplasms ; Lymph Nodes ; Male ; Melanoma ; Neoplasm Metastasis ; Nose ; Outpatients ; Paget Disease, Extramammary ; Skin Neoplasms ; Statistics as Topic ; Stomach Neoplasms ; Sunlight ; Upper Extremity

Angiogenesis is an essential process in tumor growth and metastasis. VEGF has been considered a leading candidate inducing tumor angiogenesis. VEGF expression was significantly correlated with clinical stage, lymph node matastasis, and prognosis of cancers of various parts of body. However, little has been known about the correlation between VEGF expression and clinicopathologic parameters in oral squamous cell carcinoma. The aim of this study was to correlate VEGF expression with the clinicopathological parameters and microvessel density. Forty six oral squamous cell carcinomas were analyzed using immunohistochemical method with primary antibodies to VEGF and CD31. VEGF expression was detected in 33 (71.7%) of the 46 cases. The microvessel density was significantly correlated with VEGF expression (P=0.002). There was no correlation between microvessel density and tumour size, clinical stage, and lymph node metastasis, respectively. VEGF expression did not correlate with the histological grade of tumour differentiation, tumour size, and clinical stages. The VEGF-positive rate seemed to be higher in patients with cervical lymph nodal metastasis than in those without it, but it was not statistically significant. In conclusion, the overexpression of VEGF in the oral squamous cell carcinoma seemed to be associated with a more aggressive course of the disease. Further study is necessary to define the role of VEGF in oral squamous cell carcinoma.
Antibodies ; Carcinoma, Squamous Cell* ; Humans ; Lymph Nodes ; Microvessels* ; Neoplasm Metastasis ; Prognosis ; Vascular Endothelial Growth Factor A*

The usefullness of the nitroblue tetrazolium (NBT) test as an aid in the differential diagnosis of various meningitis was evaluated in infancy and childhood. 36 cases of patients with various meningitis who were admitted to the department of pediatrics in Severance Hospital from June, 1975 to March, 1976 and 8 cases of healthy children as control were the subject of this study. The following results were observed; the mean proportion of NBT-positive neutrophils was 4.0?2.0% in healthy control group, 17.0?.7% in patients with bacterial meningitis, 6.0?.3% in tuberculous menigitis, and 5.o?,1% in aspetic meningitis. And we couclued that NBT test is useful in differenciating various form of meningitis.
Child ; Diagnosis, Differential* ; Humans ; Meningitis ; Meningitis, Bacterial ; Neutrophils ; Nitroblue Tetrazolium ; Pediatrics

No abstract available.
Anti-Bacterial Agents* ; Dysentery, Bacillary*