Objective:To investigate prevalent aeroallergens in ChongQing and their sensitization risk factors in children with allergic diseases,especially asthma.Methods:Skin prick tests with 13 standardized aeroallergens and questionnaires were used.A total of 298 patients,aged between 4~15 years and were diagnosed with allergic asthma and or allergic rhinitis,were investigated.Results:The total positive rate of allergen was 84.9%(253/298).The prevalence of aeroallergens were following:the first was house dust mite,cockroach followed,the third one was cat dander;the positive family history of asthma was 69.6%(116/230),asthma and rhinitis accounted for 67.2%(78/116)。Multivariate logistic regression analyses revealed that house dust mite was an independent risk factors for asthma(odds ratio Value(OR):2.602,95%CI:1.672～7.488),air conditioning had strong relationship with it(OR:1.659,95%CI:1.139～5.942).Conclusion:House dust mite is the major allergen of allergic diseases,especially asthma in ChongQing.Air conditioning is one of important risk factors for house dust mite sensitization.

Objective:To prepare Pingwei dispersible tablets and establish a method for the determination of atractylodin. Meth-ods:HPLC was performed on a Kromasil C18 column(250 mm × 4. 6 mm,5 μm)using methanol-water(75:25)as the mobile phase at a flow rate of 1 ml·min-1 . The detection wavelength was at 340nm. The column temperature was 30℃ and the injection volume was 10 μl. Results:The optimized formula of the tablets was as follows:the proportion of inner disintegrant microcrystalline cellulose,out-side disintegrant carboxymethyl cellulose sodium and HPMC was 40%,9% and 2%,respectively. The adhesive was 2% HPMC in 70% ethanol,and 1. 5% magnesium stearate was used as the lubricant. The linear range of atractylodin was 0. 100 6-0. 503 0 μg(r=0. 999 9),and the average recovery was 99. 3%(RSD=0. 9%,n=6). Conclusion:The formula and the preparation process of the tablets are reasonable and the content determination method is simple,accurate and specific,which can be used in the quality control of Pingwei dispersible tablets.

The patients with stroke is often accompanied by malnutrition, its causes include eating less, increased catabolism, and intestinal dysfunction, etc. Malnutrition can not only aggravate the extent of brain damage in patients with stroke, but also increase the incidence of complications, and thus affecting the course and prognosis of stroke. Therefore, correct evaluation of the nutritional status in stroke patients, choosing the appropriate nutrient timing and nutritional support methods have important significance. Among a variety of nutritional assessment methods, the scored Patient-Generated Subjective Global Assessment can quickly identify malnutrition in stroke patients. Early enteral nutrition support, including oral nutritional supplements, nasogastric tube feeding and percutaneous endoscopic gastrostomy, can significantly improve the prognosis in stroke patients. Among them, nasogastric tube feeding is the first choice for nutritional support in stroke patients.

Coronary Artery Disease ; diagnosis ; therapy ; Humans ; Tomography, Optical Coherence ; methods ; Treatment Outcome

Objective To detect the effect of Rhubarb retention enema on patients with acute pancreatitis(AP).MethodsA total of 84 AP patients were enrolled in this study. Patients were divided into the control group(n=42) and Rhubarb enema observation group(n=42). Control group was treated with fasting, continuous gastrointestinal decompression, correcting water electrolyte and acid-base imbalance, with the oral administration of octreotide and gabexatemesilate to inhibit pancreatic secretion and enzyme activity. On the basis of the control group, the observation group was treated with rhubarb retention enema. IL-17, IL-10, and IL-6 expression was detected by ELISA analysis. The clinical effect was compared.Results The clinical effect rate (85.7%vs.71.4%,χ2=2.366,P=0.043) in observation group was significantly higher than that in control group. After treatment of 7 days, IL-17 (13.53 ± 3.16 ng/mlvs. 20.63 ± 4.83 ng/ml,t=2.416) and IL-6 (22.82 ± 4.56 ng/mlvs. 31.59 ± 6.63 ng/ml,t=2.421) expression was significantly lower and IL-10 (16.32 ± 4.15 ng/mlvs. 12.17 ± 3.22 ng/ml,t=2.326) expression was significantly higher in observation group than those in control group(P<0.05). The reduction of blood amylase (125.69 ± 32.47 U/Lvs. 259.26 ± 51.44 U/L,t=7.451) and urine amylase (204.73 ± 43.83 U/Lvs. 334.25 ± 60.18 U/L,t=7.323) in the observation group was significantly higher than those in the control group(P<0.01). Abdominal pain and abdominal distension recovery time (4.24 ±1.06 dvs. 3.02 ± 0.62 d,t=2.521), exhaust gas and defecation recovery time (5.42 ± 1.25 dvs. 3.26 ± 0.73 d,t=5.124), temperature recovery time (5.63 ± 1.46 dvs. 4.58 ± 0.92 d,t=2.418), and hospitalization time (18.65 ± 4.16 dvs. 13.78 ± 3.15 d,t=2.386) were higher in control group than those in observation group (P<0.05 orP<0.01). Conclusion Rhubarb enema can regulate the IL-17/ IL-10, and inhibit the inflammatory reaction.

Objective To study the influence of serum containing Ziqi Ruangan Pill on inhibitory effect of alcohol LO2 and to investigate the regulation on apoptosis related genes and the protective mechanism for LO2. Methods Rabbit sera saline containing Ziqi Ruangan Pill was prepared. The apoptotic LO2 model was established by being cultured with alcohol. The effect of serum on the model was detected by MTT method. Identification of apoptosis was carried out using flow cytometry and Hoechst3325 fluorescent staining. The expression of apoptotic inducing gene,casepase3 and NF-?B was detected by Real-time RT PCR and Western-Blot. Results The serum containing Ziqi Ruangan Pill could regulate the expression of the casepase3,NF-?B and reduce the apoptosis. Conclusion Ziqi Ruangan Pill can protect the LO2 by regulating the expression of apoptosis stimulative gene casepase3 and NF-?B.

Lipoprotein lipase (LPL) is the rate-limiting enzyme for the hydrolysis of the triglyceride core of circulating triglyceride-rich lipoproteins.LPL can not only affect the lipoprotein level,but also play an important role in the development of atherosclerosis.LPL gene polymorphisms may affect LPL activity,and therefore have both pro-and anti-atherogenic effects.This article reviews the relationship between LPL gene polymorphism and atherosclerosis.

Moyamoya disease is a chronic progressive cerebrovascular disease. Its main manifestation is bilateral internal carotid artery progressive stenosis w ith abnormal vascular netw ork formation of the compensatory hyperplasia in brain base. The pathological mechanism of moyamoya disease is not clear. Grow ing evidence has suggested that immune inflammation may play an important role in its occurrence and development process. Abnormal expression of various inflammatory cytokines and immune proteins can be observed in patients w ith moyamoya disease. This article review s the possible mechanism of immune inflammation in moyamoya disease in recent years.

Objective To investigate the effects of CO 2 pneumoperitoneum during laparoscopic cholecystectomy (LC) on pulmonary functions in chronic pulmonary failure rabbits and their mechanisms. Methods A total of 50 healthy male rabbits ( oryctolagus cuniculus ) were randomly divided into 4 groups: normal control group (N 0: n=5, no pneumoperitoneum), experimental control group (T 0: n=5, no pneumoperitoneum), 10 mmHg experimental group (T 10 : n=20, 10 mmHg pneumoperitoneum) and 15 mmHg experimental group (T 15 : n=20, 15 mmHg pneumoperitoneum). After the successful establishment of emphysema rabbit models, CO 2 pneumoperitoneum was conducted and maintained for 2 hours at the pressure of 10 mmHg (1.33 kPa) and 15 mmHg (2.00 kPa), respectively. Pulmonary functions (total respiratory resistance, central resistance and total airway resistance) of the 4 groups were measured by pulse oscillation technique before and after the pneumoperitoneum, respectively. Results Total respiratory resistance, central resistance and total airway resistance increased after the establishment of emphysema models. And they decreased at the end of pneumoperitoneum compared with those before pneumoperitoneum ( q=17.824, P

Objective To investigate the clinical efficacy and safety of kidney stones minimally invasive percutaneous nephrolithotomy holmium laser lithotripsy.Methods 397 cases patients with kidney stones were randomly divided into the control group and the experimental group,the control group were treated with the extracorporeal shock wave lithotripsy treatment,and the later group were treated with holmium laser lithotripsy for percutaneous nephrolithotomy.Results The experimental group of taking the net rate was 97.48％,and the time of taking the net rate was 87.15 ％,higher than those of the control group(x2 =14.72,24.78,all P ＜ 0.05).Compared with the control group,the shortened operation time,(t =2.36,P ＜ 0.05),the reducing amount of bleeding (t =3.27,P ＜ 0.05),the shortened length of stay hospital (t =1.28,P ＜ 0.05),low complication rate (x2 =17.06,P ＜ 0.05) took place in the experimental group.Conclusion Minimally invasive percutaneous nephrolithotomy holmium laser lithotripsy treatment of kidney stones is safe and effective,fewer complications,faster recovery,which is worthy of promotion.