Objective To investigate the effects of repeated administrations of 6% hydroxyethy] starch (HES) 130/0.4 or 7.5 % NaCl (NS) on brain water content (BWC) after experimental intracerebral hemorrhage (ICH) in rats.Methods One hundred and sixty-seven male SD mrs weighing 260-300 g were randomly divided into 4 groups: group Ⅰ sham operation (S) (n = 20) ;group Ⅱ ICH (n = 38) ;group Ⅲ NS + ICH (n = 55) and group Ⅳ HES + 1CH (n = 54). The animals were anesthetized with intraperitoneal 10% chloral hydrate 400 mg/kg. Experimental ICH was produced by injection of fresh autologous blood 50 μl into right caudate nucleus. In group NS (group Ⅲ) 7.5% NaCl 5 ml/kg and in group HES (group Ⅳ) 6% HES 30 ml/kg were injected at 2, 24, 48 and 72 h after ICH. In group S and ICH (group Ⅰ and Ⅱ) 5 animals were killed at 2, 24, 48 and 72 h after ICH, while in group Ⅲ (NS) and Ⅳ (HES) 5 animals were killed at 2, 24, 48 and 72 h after ICH, immediately before and after NS/HES infusion for determination of ipsilateral and contralateral cerebral cortex and basal ganglion, weight and W/D weight ratio. Rosenberger behavior scores and survival rates were also recorded. Results In group Ⅲ (NS + ICH) and Ⅳ (HES + ICH) the water content of ipsilateral cerebral cortex and basal ganglion was significantly lower than in group Ⅱ (ICH). Rosenberger behavior scores at 24 h and 48 h after ICH were significantly lower in group NS and HES than in group Ⅱ (ICH) . The survival rates at 24, 48 and 72 h after ICH were significandy lower in group Ⅱ than in group NS and HES, especially at 72 h in group HES. Conclusion Repeated administrations of 6% HES 130/0.4 or 7.5% NaCl can ameliorate brain edema after ICH.

Objective To observe the effects of repeated dosing of 6% hydroxyethyl starch (130/0.4) or 7.5% sodium chloride on brain edema after experimental intracerebral hemorrhage (ICH) in rats. Methods 167 male SD rats were divided into four groups randomly: Sham operation group (S, n=20), ICH control group (M, n=38), 7.5% sodium chloride group (N, n=55) and 6% hydroxyethyl starch group (H, n=54). The model of the ICH was established with stereotactically infusing 50 μl of the autologous femoral artery blood into the right caudate nucleus. group N and group H received 7.5% sodium chloride 5 ml/kg and 6% hydroxyethyl starch 30 ml/kg at 2 h, 24 h, 48 h and 72 h after operation respectively. The tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), superoxide dismutase (SOD) and malondialdehyde (MDA) in the tissue around the hemorrhage were measured at different time point. Results The IL-6 in group N was significantly more than that in group M at 24 h and 72 h after infusion (P<0.05), and the TNF-α in group H was less than that in group M at 24 h and 48 h after infusion (P<0.05). The SOD in group M decreased to the bottom at 48 h and 72h after ICH. SOD in group N and group H at 24 h, 48 h and 72 h after infusion was both significant more than that in group M (P<0.05). MDA in group H at 72 h after infusion was less than that in group M (P<0.05). Conclusion Repeated infusion of 6% hydroxyethyl starch (130/0.4) or 7.5% sodium chloride can decrease inflammatory response of brain tissue after ICH, which may protect brain from oxidative damage.

Objective To evaluate the performance of bispectral index (BIS),auditory evoked potential index (AEPI) and cardiovascular response to electrical tetanus stimulus (ETS) for monitoring depth of sedation induced by propofol , analgesia produced by fenanyl and depth of isoflurane anesthesia. Methods Twenty ASA Ⅰ or Ⅱ patients of both sexes (11 males,9 females) aged 18-56 yr, weighing 50-80 kg undergoing elective brain surgery were studied. The patients were unpremedicated. The baseline BIS, AEPI, BP(SP, DP) and HR were measured before anesthesia. Propofol was infused at 0.25 mg?kg-1?min-1 and the infusion was stopped when the total amount of propofol infused reached 5 mg?kg-1 at the end of 20 min infusion. Fentanyl 2 ?g?kg-1 was then given i.v. Three minutes later the patients were intubated and mechanically ventilated. Tracheal intubation was facilitated by vecuronium 0.1 mg?kg-1. Anesthesia was maintained with isoflurane. Depth of sedation was measured using OAA/S scale during propofol infusion. ETS was given at the following time points: when the amount of propofol infused reached 2.5, 3.0, 3.5, 4.0, 4.5, 5.0 mg?kg-1,2 min after fentanyl administration and at 0.7, 1.0, 1.3 MAC isoflurane (end-tidal isoflurane concentration). BIS and AEPI values before and after ETS and changes in BP and HR (ABP, AHR) after ETS and the time for BP and HR to return to the pre-ETS value in 50% and 90% of the patients (TBP50%,TBP90%,THR50%,THR90% ) were recorded. Results BIS and AEPI were positively correlated with sedation depth. There were no significant changes in BIS and AEPI after ETS, but SP, DP and HR increased significantly after ETS( P

Objective To investigate the effect of lidocaine on plasma S100β protein in patients with supratentorial tumor surgery.Methods Thirty patients with supratentorial tumor surgery were selected,ASA grade Ⅰ-Ⅱ grade.The patients were divided into lidocaine group and control group by random digits table with 15 cases each.In lidocaine group,a dose of 2％ lidocaine was administered as an intravenous bolus (1.5 mg/kg) after induction,followed by an intravenous infusion at rate of 2 mg/ (kg ·h) until the end of surgery.In control group,0.9％ sodium chloride was given in the same volume and the same rate.Bloods were sampled from bulb of jugular vein to measure the plasma S100β protein before surgery,end of surgery and 1 day after surgery.Results The plasma S100 β protein before surgery,end of surgery and 1 day after surgery in lidocaine group were (21.03 ± 11.67),(32.63 ± 10.14) and (34.16 ± 17.59) ng/L,in control group were (23.04 ± 13.32),(44.98 ± 16.63) and (39.85 ± 21.99) ng/L.There were no statistical differences in the plasma S100 β protein before surgery and 1 day after surgery between the 2 groups (P ＞ 0.05),but there was statistical difference end of surgery between the 2 groups (P ＜ 0.05).Conclusion Intraoperative infusion of lidocaine can decrease the plasma S100β protein end of supratentorial tumor surgery.

Nuclear factor κB (NF-κB) is an important cellular transcription factor. The important role of NF-κB-mediated cell signal transduction pathway in apoptosis is a hot topic at home and abroad. In order to discover new regulators in NF-κB signaling pathway, a high-throughput cell-based screening model based on dual luciferase reporters system was established, a number of genes that can activate NF-κB signal pathway were obtained by screening of 439 novel function genes. Among them, TMEM9B can obviously activate NF-κB signaling pathway. Further experiments showed that TMEM9B activated NF-κB signaling pathway in a dose-dependent pattern. Western blotting and EMSA experiments confirmed that TMEM9B can promote the degradation of IκBα (a cytoplasm inhibitor of NF-κB), and cause NF-κB shift from the cytoplasm to nucleus. At the same time, flow cytometry result demonstrated TMEM9B can induce apoptosis in HEK293T and HeLa cells. In short, a stable and effective screening system for NF-κB has been established, through which TMEM9B was identified to be able to significantly activate NF-κB signal transduction pathway and thus cause cells apoptosis.

Alzheimer’ s disease ( AD) , the most prevalent type of neurodegenerative disease, characterized by extracellular β-amyloid ( Aβ) plaque deposition, intracellular neurofibrillary tangles, and the loss of diverse neurons. Neuron loss might be the main cause that induces irreversible decline of cognitive function in AD patients. At present, AD therapy only relieves symptoms instead of fundamentally affecting the major pathologi-cal characteristics of the disease. Recently, rapid advances in neurogenesis and stem-cell biology have provided a new and pro-spective potential for AD treatment. Stem-cell types in the treat-ment of neurodegenerative diseases include neural stem cells ( NSCs ) , embryonic stem cells ( ESCs ) , mesenchymal stem cells ( MSCs ) , and induced pluripotent stem cells ( iPSCs ) . Stem cell therapy shows anti-AD function by replacing the dam-aged and lost neurons, rebuilding the cellular loop, inhibiting amyloidogenesis, promoting neurotrophic factor release, and reg-ulating immune reaction. This review highlights the recent pro-gress, mechanisms, and preclinical evidence of exogenous trans-planted stem cell, and addresses the current major challenges of stem cell transplantation in clinic. In addition, this review also summarizes the research status of compound promoting endoge-nous neurogenesis in brief.

Objective To study the influence of carboxymethyl-chitosan (CM-chitosan) on phenotype and proliferation of chondrocytes stimulated by recombinant human interleukin-1? (rhIL-1?), and explore its mechanism. Methods Chondrocytes were isolated and cultured. 10 ng/ml IL-1? with or without CM-chitosan of varied concentrations were added into the culture medium. After 24 h, changes of proliferative ability of chondrocytes were tested by MTT and flow cytometry. Proteoglycan synthesis was measured by incorporation of Na235SO4 into chondrocytes, nitric oxide (NO) production was detected by Greiss reaction, mRNA expression of typeⅠ、Ⅱcollagens, Aggrecan and inducible NO synthase (iNOS) were examined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results CM-chitosan could significantly antagonize IL-1?-induced inhibitory effect on proliferation of chondrocytes, restore proteoglycan synthesis of chondrocytes, decrease NO production of IL-1?-induced chondrocytes, increase the mRNA expression of typeⅡcollagen and Aggrecan, but decrease the expression of typeⅠcollagen and iNOS. The effects of CM-chitosan were in a dose-dependent manner. Conclusion CM-chitosan can maintain the proliferation and phenotype of IL-1?-induced chondrocytes.

Objective To explore teaching effect of comprehensive teaching mode of PBL combined with CBL in ophthalmologic cataract clinical teaching.Methods 2008 grade clinical majors (n =80) in class 1 as experimental group were taught by comprehensive teaching mode of PBL combined with CBL while those (n =83) in class 2 as control group were taught by traditional LBL teaching mode.After the courses,the teaching effects of two methods were compared.SPSS 11.5 software was used for statistical analysis,x2 test for satisfaction survey and t-test for theoretical examination scores.The test level is α =0.05.Results There were significant differences between experimental group and control group in improving comprehensive quality and developing clinical thinking.Scores of understanding knowledge,case analysis and total scores of experimental group were higher than those of control group (all P ＜ 0.05).Conclusions Comprehensive teaching mode may improve the teaching effect of cataract clinical teaching,but it need to be explored and improved continually in practice.

Objective To study the clinical characteristics of primary Sj(o)gren's syndrome (pSS) patients with dry eyes.Methods Ninety-six pSS patients with dry eyes admitted to the Department of Rheumatology and Immunology,the Affiliated Hospital of Guilin Medical College from August 2009 to August 2011 were retrospectively analyzed.Results In all 96 patients,89 patients had symptoms,23 of whom had two or more symptoms.The tear-film break-up time (BUT) of 154 eyes were Less than 5 seconds,18 were between 5-10 second,and twenty were more than 10 seconds,with the average of 3.7±1.9 seconds.The length of tear flow of 154 eyes'width of tear mocus was less than 3 millimetres (mm),with the average of (0.21±0.10) mm.The average score of corneal fluorescein staining score was 5.2±2.4.The wet lengths of filter paper of 192 eyes were all less than 10 mm/5 min,with the average of (4.6±1.8) mm.The average score of meibomian gland function was 1.5±0.9.Conclusion In patients with pSS,the width of tear meniscus and the wet lengths of filter paper are reduced,which implies less tear secretion.At the same time,BUTs are short,and the Meibomian gland dysfunction are observed.Dry eyes of pSS are mixed in type,not only aqueous tear deficiency.

Objective To investigated the different effect of moderate-intensity continuous training (MCT) and high-intensity interval exercise training (HIT) on ventricular remodeling and mitochondrial homeostasis after acute myocardial infarction (AMI).Methods The AMI rat model was achieved by ligating coronary artery.The AMI and sham operation rats were randomly (random number) divided into four groups:sham operation group (Sham),AMI control group (AMI),AMI MCT group (AMI + M),and AMI HIT group (AMI + H).Animals in the AMI + M and AMI + H groups underwent 4 weeks MCT and HIT respectively.Five weeks after AMI,hemodynamic changes,mitochondrial bioenergetics,and PINK1,Beclinl,Mfn2,Drp1,Tfam,COXⅣ,PGC-1α were detected.Results Comparing with AMI group,in AMI + M and AMI + H groups,Beclin1,PINK1,Mfn2 and PGC-1α expression elevated significantly (P ＜0.05 or P ＜0.01),whereas ROS generation and Drp1 expression showed dramatic decrease (P ＜ 0.05 or P＜0.01).In addition,in AMI + H group,±dp/dt max,mitochondrial membrane potential,ATP synthesis activity,Tfam and COXⅣ expression improved significantly (P ＜ 0.05).Comparing with AMI + M group,in AMI + H group,± dp/dt max,PGC-1α,Tfam and COX Ⅳ expression improved significantly (P ＜ 0.05).Conclusions HIT is superior to MCT for ameliorating ventricular remodeling and mitochondrial homeostasis after AMI.