Objective To get knowledge of the indoor air pollution states in the newly renovated apartments in Xi’an city, and explore the relationship among the major pollutants and the relationship between the pollutants and the differences of seasons. Methods From January, 2006 to May, 2007, we randomly selected 138 newly renovated (3 months or less) apartments in the urban area of Xi’an city, tested their indoor (including bedrooms, living room, dining room, kitchen, bathrooms, study, a total of 597) content of formaldehyde, total volatile organic compounds (TVOCs), ammonia, benzene, toluene and xylene. Results Among which were detected in all of the pollutants, total volatile organic compounds (TVOCs) and formaldehyde own the highest exceeding rates, respectively 69.8% and 60.8%. They are followed by ammonia, whose exceeding rate is 13.2%, xylene which has the highest exceeding rate among the benzene compounds, 8.7%. We did comparative analysis of the concentration in different types of rooms for the pollutants which are among the top four in exceeding rate ranks, and found that only for formaldehyde, there is significant difference in statistics (P

Indoor microbial contamination of the buildings and the related health impacts has been more and more concerned by the public.The indoor microbial contaminants contain bacteria,virus,fungus,dust mites,pollen and so on,and the source is diversity.Many studies at home and abroad have found that the microbial contaminants in different kinds of the buildings like the public places,schools,living rooms and so on are very popular,sometimes quite high level.Indoor microbial contamination can cause adverse impact on health of the exposed population,such as some infectious diseases,allergenic symptoms/diseases,even cancers.

0.05). ALP activity of the cells on deproteinized bone was higher than that on decalcified bone(P

Objective To investigate the association between the ATP-binding cassette transporter A1(ABCA1)gene polymorphisms and plasma high density lipoprotein cholesterol(HDL-C)level in Chinese general population.Methods M883I,R1587K and R219K polymorphisms of the ABCA1 gene were detected by polymerase chain reaction-restriction fragment length polymorphism technique in 376 unrelated Chinese healthy subjects.Results In the study population,the subjects with the KK1587 genotype had markedly lower HDL-C levels than those with RR1587 and RK1587 genotypes(P

Resveratrol is a natural polyphenolic substance with a variety of bioactivities,such as anti-oxidation,anti-tumor and apoptosis induction.This paper summarized the recent research progress of resveratrol induced cancerous cell apoptosis,including human cervical cancer HeLa cells,leukemia cells,hepatoma cells and gastric cancer cells,and analyzed the molecular mechanism behind resveratrol-induced cell apoptosis in tumor.In addition,resveratrol was investigated by blocking the cell cycle,regulating the expressions of related genes and proteins and mitochondria-induced apoptosis pathways as a potential application of cancer drugs to clinical researches.

OBJECTIVETo evaluate whether the vascular endothelial growth factor (VEGF)-C cDNA which cloned from a patient with squamous cell carcinoma (SCC) of tongue can encode a functional protein or not.

METHODSRT-PCR was employed to clone the functional VEGF-C fragment from the surgical specimen of a lingual SCC patient. Then it was subcloned into expressive plasmid vector pBKCMV, which was transfected into E. coli to examine its expression.

RESULTSA truncated human VEGF-C cDNA fragment was amplified from the lingual SCC. The sequencing results of the fragment demonstrated that it had 99.6% similarity with the reported human VEGF-C cDNA (representing the 559-1611 bp according the sequence of Genbank Entry X94216). Induced with IPTG, the E. coli XLI-Blue MRF' containing the recombinant pBK-VEGF-C expressed a 56,000 fusion protein, which can be recognized by polyclonic anti-human VEGF-C antibody.

CONCLUSIONA functional fragment VEGF-C cDNA was cloned from a lingual SCC. It will promote more intensive research on the function of VEGF-C and its relationship with metastasis of oral SCC.

Carcinoma, Squamous Cell ; genetics ; metabolism ; Cloning, Molecular ; DNA, Complementary ; biosynthesis ; genetics ; Escherichia coli ; genetics ; Humans ; Lymphatic Metastasis ; Prokaryotic Cells ; metabolism ; Tongue Neoplasms ; genetics ; metabolism ; Transfection ; Vascular Endothelial Growth Factor C ; biosynthesis ; genetics

OBJECTIVEA functional VEGF-C cDNA was cloned from a patient with squamous cell carcinoma (SCC) of tongue in order to study the important role of vascular endothelial growth factor (VEGF)-C in lymphatic dissemination of malignancies in gene level.

METHODSRT-PCR was employed to clone the human VECF-C encoded cDNA from a surgical specimen of a lingual SCC patient. Then it was subcloned into plasmid vector pCRII and sequenced.

RESULTSA 1.1 kb human VEGF-C cDNA fragment was amplified from the lingual SCC. The sequencing results of the fragment demonstrated that it had 99.6% similarity with the reported human VEGF-C cDNA (representing the 559-1,611 bp according the sequence of Genbank Entry X94216).

CONCLUSIONAn encoded fragment VEGF-C cDNA was successfully cloned from a lingual SCC and provided a necessary material for further study.

Carcinoma, Squamous Cell ; genetics ; Cloning, Molecular ; DNA, Complementary ; genetics ; Humans ; Sequence Analysis, DNA ; Tongue Neoplasms ; genetics ; Vascular Endothelial Growth Factor C ; genetics

OBJECTIVETo study the role that vascular endothelial growth factor-C plays in the lymph node metastasis of the squamous cell carcinoma (SCC) of tongue.

METHODSAn established VEGF-C transfected Tca8113 cell strain (VcTca) was heterotransplanted into BALB/C nude mice subcutaneously. The expression of VEGF-C and VEGFR-3 was detected. The property of tumorigenesis and metastasis of the transfected cell was investigated.

RESULTSJugular lymphatic metastases developed in 4/6 mice of VcTca group in 6 weeks after transplantation, while no lymph node metastasis was found in mice with tumors of non-transfected Tca8113 cell (Tca) and mock transfected cell(pDTca) at the same time. There was no difference in tumor weight among VcTca, pDTca and Tca (P > 0.05).

CONCLUSIONIt is suggested that VEGF-C could promote lymph node metastatic ability of Tca8113 cell, and could be utilized as a target of new regimen in prevention and treatment for lymphatic metastasis of oral carcinomas.

Animals ; Carcinoma, Squamous Cell ; genetics ; pathology ; Lymphatic Metastasis ; prevention & control ; Male ; Mice ; Mice, Inbred BALB C ; Neoplasm Transplantation ; Random Allocation ; Tongue Neoplasms ; genetics ; pathology ; Transfection ; Vascular Endothelial Growth Factor C ; genetics

Objective To study the sleep improvement function of DHA-PC.Methods The mice were randomly divid-ed into control, vehicle, DHA+Lecithin (60+200 mg/kg) and DHA-PC(50,100,200 mg/kg) groups.Ten mice were enrolled in each group .The mice of control were administered with normal food , the vehicle group was orally given normal saline at the dosage of 0.2 ml/10 g, while both DHA-PC and DHA+Lecithin were orally given corresponding drugs at the dosage of 0.2 ml/10 g.All the groups were treated for 30 days except control group .The direct sleep-inducing test, the test of lengthening sleep time induced by pentobarbital sodium , the test of pentobarbital sodium subthreshold-hypnosis and the test of barbital sodium sleep latency were conducted to observe the inductive effect of DHA -PC.Results Neither the effect on mice body mass nor directly-induced sleep was observed .DHA-PC (50,100, and 200 mg/kg) could prolong sleep time to (56.2 ±13.7),(57.9 ±25.4) and(64.1 ±18.4) min, respectively,compared to vehicle(32.9 ±10.8)min (P<0.05).DHA+Lecithin could not prolong sleep time (38.6 ±11.7)min compared to (32.9 ±10.8)min of vehicle.There was significant difference compared with DHA-PC at the dosage of 200 mg/kg (64.1 ±18.4)min (P<0.05).DHA-PC (200 mg/kg) enhanced pentobarbital sodium subthreshold-hypnosis (70%) compared to vehicle (10%) (P<0.05),so did DHA+Lecithin (60%) compared to vehicle (10%) (P<0.05).Both DHA-PC (200 mg/kg)[(22.9 ±4.1)min ] and DHA+Lecithin [(19.5 ±2.7) min ]could shorten sleep latency compared to vehicle (31.3 ±6.9) min(P<0.01), and the sleep latency of DHA +Lecithin (19.5 ±2.7) min was shorter than that of DHA-PC(50,100 mg/kg).Conclusion DHA-PC has some effect some sleep improvement in mice .

Hypoxia-inducible transcription factor-1alpha (HIF-1alpha) is a "master" gene in the cell hypoxia response network. HIF-1alpha plays the key role on VEGF-dependent angiogenesis during tumor development. In this study, the siRNA expressive vector targeting HIF-1alpha was constructed. HepG2 cells were tranfected with an HIF-1alpha siRNA vector and exposed to a normoxic or hypoxic environment. mRNA and protein levels of HIF-1alpha and VEGF were detected by RT-PCR and Western blot at different cell culture environment. The mRNA and protein expression of HIF-1alpha was inhibited by RNA interference. The obvious decreases of mRNA and protein of VEGF was induced by HIF-1alpha gene knock-down. Our study indicated that HIF-1alpha regulated the expression of VEGF and hence promoted angiogenesis in liver cancer under hypoxic environment. The expression of VEGF was downregulated via HIF-1alpha gene knock-down. These results suggest that HIF-1alpha may be used as a good target gene for anti-angiogenesis in liver tumors.
Carcinoma, Hepatocellular ; metabolism ; pathology ; Cell Line, Tumor ; Down-Regulation ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit ; genetics ; Liver Neoplasms ; metabolism ; pathology ; RNA Interference ; RNA, Messenger ; metabolism ; RNA, Small Interfering ; genetics ; Vascular Endothelial Growth Factor A ; metabolism