Objective:To study on effect of Danshao Huaxian Capsule on proliferation of activated hepatic stellate cells(HSCs)in vivo and in vitro.Methods:(1)In vitro experiment:57 male Wistar rats were divided into a normal group,a model group and a treatment group.The rats of model group and treatment group were made hepatic fibrosis model with complex stimulation of CCl_4,drinking alcohol,high lipids and low protein diet for 8 weeks.The treatment group were treated for 8 weeks with intragastric perfusion of Danshao Huaxian Capsule,1g/kg.At the end of experiment,a part of the rats were used for detection of hepatic functions and hepatic fibrosis degrees,and another part of the rats were used to separate HSCs.Cell cycle percentage was detected with flow cytometry.(2)In vivo experiment:prepare Danshao Huaxian Capsule serum of the rat;separate cultured HSCs of the rat and divide into calf serum group,normal rat serum group and Danshao Huaxian Capsule serum group.5%,10% and 20% of the above serum was respectively added into the cultured HSCs.Primary HSCs proliferation was detected with MTT method.Results:(1)In vitro experiment:The hepatic function,hepatic fibrosis level and HSCs proliferation reduced significantly.(2)In vivo experiment: Danshao Huaxian Capstde serum group could significantly inhibit proliferation of HSCs as compared with calf serum group and normal rat serum group at the same content.The action showed dose-dependence.Conclusion:Danshao Huaxian Capsule can obviously inhibit proliferation of HSCs in vivo and in vitro.

Objective To study the effect of Snsong Yangxin capsule and metoprolol sustained release tablet on the curative effect and the levels of BNP and HR in the elderly coronary heart disease (CHD) arrhythmia patients. Methods It selects 108 patients with coronary heart disease and arrhythmia in elderly patients were divided into observation group (54 cases) and control group (54 cases). The control group was treated with metoprolol sustained-release tablets, and the observation group was treated with Shensong Yangxin capsule and metoprolol sustained-release tablets. The curative effect of two groups and the changes of BNP and HR levels before and after treatment were observed and compared. Results The treatment effect, times of contraction, frequency of cardiac fibrillation, frequency of defect, load, time, blood pressure and BNP of the observation group were significantly better than those of the control group (P<0.05). Conclusion For elderly patients with coronary heart disease arrhythmia, the treatment of Shensong Yangxin capsule combined with metoprolol sustained release tablets is effective. It can significantly improve the patient's heart rate, restore the blood supply to the heart, improve the blood pressure and BNP, with less adverse reactions, safe and reliable, and worthy of promotion.

OBJECTIVE:To observe the clinical efficacy of oral traditional Chinese medicine concomitant with artificial liver in the treatment of severe type hepatitis.METHODS:A total of176patients were randomly divided into the treatment group and control group:the control group was treated with conventional internal medicine therapy together with artificial liver,while the treatment group was treated with additional traditional Chinese medicine besides the therapy for the control group.And the course of treatment was2weeks.RESULTS:The cure rates for the treatment group and the control group were45.5%and20.5%,respectively(P

With the development of imaging techniques, aphasia caused by subcortical impairment is increasingly found in recent years. This paper reviewed the literature about the subcortical aphasia and expolred the anatomical and clinical features and pathogenesis of subcortical aphasia.

Objective:To investigate the roles of Th2 cytokine receptors in the pathogenesis of systemic lupus erythematosus(SLE) and its clinical significance.Methods:RT-PCR technique was used to semiquantitatively analyze Th2 cytokine receptors mRNA expression in PBMC from 89 patients with SLE,double antibody sandwich ELISA was applied to determine Th2 cytokines in serum.Results:The positive Th2 cytokine receptors mRNA expression accounted for 100% among active SLE group(group Ⅰ),inactive SLE group(group Ⅱ) and control group(group Ⅲ).The mean levels of IL-4R mRNA expressions in group Ⅰ,Ⅱ and Ⅲ were 0.604?0.147,0.40?0.13 and 0.37?0.07,respecitively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-4R mRNA expressions in group Ⅰ was markedly increased(P=0.00),while there were no statistical differences between group Ⅱ and group Ⅲ(P=0.351).The mean levels of IL-6R mRNA expressions in group Ⅰ,Ⅱ and Ⅲ were 0.902?0.273,0.519?0.11 and 0.573?0.24,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-6R mRNA expressions in group Ⅰ was markedly increased(P=0.00),while there were no statistical differences between group Ⅱ and group Ⅲ(P=0.289).The mean levels of IL-10R mRNA expressions in group Ⅰ,Ⅱ and Ⅲ were 0.87?0.29,0.722?0.209 and 0.684?0.138,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-10R mRNA expressions in group Ⅰ was markedly increased(P=0.00),while there were no statistical differences between group Ⅱ and group Ⅲ(P=0.405).The mean levels of IL-4 in serum in group Ⅰ,Ⅱ and Ⅲ were 105.57?29.17,51.17?16.69 and 23.73?6.24,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL(4 in group Ⅰ was significantly higher than those in group Ⅱ and group Ⅲ(P=0.00),while that in group Ⅱ was higher than group Ⅲ(P=0.00).The mean levels of IL-6 in serum in group Ⅰ,Ⅱ and Ⅲ were 71.89?20.02,17.96?6.93 and 0.035?0.035,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-6 in group Ⅰ was significantly higher than those in group Ⅱ and group Ⅲ(P=0.00),while that in group Ⅱ was higher than group Ⅲ(P=0.00).The mean levels of IL-10 in serum in group Ⅰ,Ⅱ and Ⅲ were 32.32?11.51,18.66?6.63 and 0.289?0.867,respectively.Compared with group Ⅱ and group Ⅲ,the mean level of IL-10 in group Ⅰ was significantly higher than those in group Ⅱ and group Ⅲ(P=0.00),while that in group Ⅱ was higher than group Ⅲ(P=0.00).There were positive correlation between IL-4 and IL-4R in active group and inactive group(r=0.622-0.859,P

Objective To investigate the relationship between the severity of diabetic retinopathy and serum bilirubin level. Methods From August 1 2008 to December 31 2009, hospitalized patients of 1062 cases with type 2 diabetes were divided into three groups according to fundus examination: no diabetic retinopathy (NDR) group (457 cases), non-proliferative diabetic retinopathy (NPDR) group (484 cases)and proliferative diabetic retinopathy (PDR) group ( 121 cases). The difference in serum bilirubin level was compared. Results Total bilirubin in PDR, NPDR and NDR group were (9.96 ± 3.84), ( 11.41 ± 3.82),( 13.38 ± 3.98) μ mol/L respectively. There was significant difference among three groups (P ＜ 0.01 ). Logistic multivariate regression analysis showed that a drop in serum total bilirubin level was an independent risk factor of diabetic retinopathy. Conclusions Bilirubin level and the severity of diabetic retinopathy have some relationship. The antioxidant bilirubin and anti-inflammatory effect should be paid attention to.

Objective To explore the therapeutic efficacy of the water soluable photosensitizer D-galactopyranosyl zinc phthalocyanines (T1)-mediated photodynamic therapy (PDT) applied to HepG2 human hepatocellular carcinoma cells in vitro and in vivo,as well as its mechanism.Methods HepG2 cells in their logarithmic growth phase were cultured and divided into different concentrations ofT1 (0 μM,0.06 μM,0.125 μM,0.25 μM,0.5 μM and 1 μM).Methyl thiazolyl tetrazolium (MTT) assays were employed to determine the effect of the T1-PDT on the proliferation of the HepG2 cells.Cell apoptosis and necrosis were measured using a cell analyzer with Annexin VFITC/PI/Hochest33342 triple-staining.The reactive oxygen species (ROS) and the mitochondrial membrane potentials of the HepG2 cells were detected using fluorescence microscopy.Confocal microscopic assays were used to observe T1's subcellular localization on the HepG2 cells.Real-time quantitative polymerase chain reactions (RT-PCRs)were used to detect any apoptosis of Bcl-2-and Bax-related genes.H-22-bearing mice were used to calculate the antitumor rate of T1-PDT,and the expression levels of Bcl-2 and Bax mRNA were detected using RT-PCRs.Twenty-four healthy mice were randomly divided into a control group,a low-dose group,a middle-dose group and a high-dose group,each of 6.Each group was given different doses of T1-PDT and the tumor inhibition rate was calculated.Results The MTT assays showed that T1-PDT could significantly inhibit HepG2 cell growth,but T1 or PDT alone had little effect.The confocal microscope assay showed that T1 was mainly localized in the mitochondria in HepG2 cells with little in the lysosome.Cell analyzer results showed that T1-PDT could induce HepG2 apoptosis.The ROS levels of HepG2 cells increased after T1-PDT.The RT-PCR results showed that T1-PDT could increase the expression of Bax and decrease the expression of Bcl-2.The in vivo experiments demonstrated that T1-PDT significantly inhibited the growth of H-22-xenografied tumors.Conclusions T1-mediated PDT has a significant lethal effect on HepG2 cells in vitro and in vitro.The lethal effect of PDT on cancer cells is shown in the apoptosis and can be attributed to T1's subcellular localization in the mitochondria,increasing ROS levels,and regulating apoptosis-related genes.

Objective To investigate the type 2 diabetic patients with glomerular filtration rate and urinary albumin value as renal finction evaluation.Methods A total of 1217 patients with type 2 diabetic nephropathy from August 1,2010 to June 31,2012 were selected.Evaluated glomerular filtration rate (eGFR) was calculated according to the currently recommended simplified modification of diet in renal disease study (MDRD) formula.According to the K/DOQI guidelines for chronic kidney disease,patients were divided into three groups:group A [eGFR ≥90 ml/ (min ·1.73 m2)] of 891 cases,group B [60 ml/(min· 1.73 m2) ≤eGFR ＜ 90 ml/(min· 1.73 m2)] of 256 cases,group C[eGFR ＜ 60 ml/(min· 1.73 m2)]of 70 cases,respectively.Eight-hour urinary albumin excretion rate (UAER) was compared between and among the groups; according to 8-hour UAER,patients were divided into three groups:group a (8-hour UAER ＜20 μg/min) of 946 cases,group b (20 μg/min≤8-hour UAER ＜200 μg/min) of 193 cases,group c(8-hour UAER ≥ 200 μ g/min) of 78 cases.eGFR was calculated and compared between and among the groups.Results Eight-hour UAER was (32.71 + 79.62),(57.56 + 130.59),(107.50 + 222.64) μ g/min in group A,B,C,and the difference among three groups was statistically significant (P＜0.05).eGFR was (110.78 ±32.75),(108.26 ±45.78),(94.96 ±32.30) ml/ (min ·1.73 m2) in group a,b,c,and the difference between group a and group c,or between group b and group c was statistically significant (P ＜0.05),but the difference between group a and group b was not statistically significant (P ＞0.05).Conclusion When evaluating the function of type 2 diabetic nephropathy,two indicators should be considered:glomerular filtration rate and urinary albumin.

Objective To study the relationship between urinary albumin excretion rate(UAER)and cardiac structure and function in type 2 diabetes mellitus(T2DM).Methods Five hundred and twenty patients with T2DM were divided into three groups by 8 h UAER:normoalbuminuria group(424 cases),microalbuminuria group(75 cases)and macroalbuminuria group(21 cases).At the same time,50 healthy people were selected as control group.The cardiac structure and function in four groups were evaluated by echocardiographic measurements.Results Left atrial dimensio[(34.39±3.46)mm],left ventricular posterior wall thickness[(10.34±1.05)mm],relative wall thickness(0.46±0.05),interventricular septal thickness[(10.49±1.07)mm],left ventricular mass[(167.37±32.12)g],left ventricular mass index[(87.62±12.16)g/m2]in macroalbuminuria group were significantly higher than those in control group[(32.59±2.71)mm,(9.30±0.77)mm,0.42±0.04,(9.75±0.81)mm,(147.33±27.23)g,(80.14±13.81)g/m2](P＜0.05),mean while the ratio of E/A(0.82±0.19)in macroalbuminuria group was significantly lower than that in control group(1.21±0.16)(P＜0.05).Conclusions In T2DM patients with albuminuria,the cardiac structure and function have changed.With the increase of UAER,the impairments are more prominent.And it may predict diabetes cardiomyopathy.

Objective To explore the necessity and effectiveness of type-based acupuncture treatment for cervical spondylosis. Method Two hundred and forty patients with clinically common cervical spondylosis, cervical spondylotic radiculopathy or cervical spondylotic vertebral arteriopathy, 80 cases each type, were enrolled. Every type of patients was randomly allocated to observation and conventional groups. The observation group (120 patients) received type-based acupuncture treatment. Cervical spondylosis was treated by syndrome differentiation; cervical spondylotic radiculopathy, from the spleen and stomach; cervical spondylotic vertebral arteriopathy, with nape five-needle acupuncture. Every type of the conventional group (120 patients) received the same therapeutic method (conventional selection of acupoints). Before and after five and ten sessions of treatment, cervical spondylosis and cervical spondylotic radiculopathy were scored using the CSAS and the VAPS and cervical spondylotic vertebral arteriopathy was scored using the ESCV. The overall therapeutic effects were evaluated after ten treatments. Result Of the patients with cervical spondylosis, there were statistically significant pre-/post-treatment differences in the CSAS and VAPS scores in the two groups (P<0.05, P<0.01) but no statistically significant differences in the scores between the groups (P>0.05). Of the patients with cervical spondylotic radiculopathy, there were statistically significant differences in the CSAS and VAPS scores in the two groups after five treatments compared with before (P<0.05) but no statistically significant differences in the scores between the groups (P>0.05);after ten treatments there were statistically significant differences in the scores in the two groups compared with before (P<0.01) and also between the groups (P<0.05). Of the patients with cervical spondylotic vertebral arteriopathy, there was a statistically significant difference in the ESCV score in the observation group after five treatments compared with before (P<0.01);the score also decreased in the conventional group (P<0.05);there was a statistically significant difference between the groups (P<0.05);after ten treatments there was a statistically significant difference in the ESCV score in the two groups compared with before (P<0.01) and also between the groups (P<0.05). After ten treatments, the cure and marked efficacy rate and the total efficacy rate were 76.6%and 92.8%, respectively, in the observation group and 54.2%and 86.0%, respectively, in the conventional group. There was a statistically significant difference in the cure and marked efficacy rate between the two groups (P<0.01). Conclusion Type-based acupuncture can markedly improve the clinical therapeutic effect on cervical spondylosis.