Actovegin is a drug,which can improve cellular energy metabolism,especially glycometabolism.It has an insulin-like effect,such as increasing the uptake of glucose and activating glucose metabolic enzyme.In addition,it can inhibit the production of lactic acid.Actovegin was mainly applied to the treatment of Alzheimer disease initially,but now it is indicated more and more.

Objective To study the expression of HDAC2 in lung of smoke cessation and smoking cessation rats,Methods Forty SD rats were divided into five groups randomly:normal control group (group A),1-month smoking group (group B,),2-month smoking group (group C),1-month smokingcessation group(group D),2-month smoking-cessation group(group E).Group B rats were exposured to cigarettes for 1 month,C,D,E were exposured to cigarettes for 2 months.At 1 month,group B were sacrificed,at 2 month,group C were sacrificed,group D was smoking cessation for 1 month and group E for 2 months.Pathomorphological changes of the small airway were analyzed,and then study the HDAC2 in rats'lung tissue.Results Compared with group A,the levels of HDAC2 in group B,group C,group D,group E were decreased (P ＜ 0.01),but smoking groups levels were lower than smoking-cessation groups.In smoking groups,group C was lower than group B.In smoking-cessation groups,group D was lower than group E.Conclusion It shows that the levels of HDAC2 in rats' lung tissue decrease after smoking exposure.It can recrease after quitting,but still cant back to normal.

Prostate cancer is one of the most common malignant tumors in males. Endocrine therapy is currently the main treatment for patients with advanced prostate cancer. Although this therapy frequently results in tumor shrinkage, it is not curative, and the majority of patients eventually develop hormone-refractory prostate cancer. Recent studies suggested that prostate cancer stem cells serve a key function in the occurrence, development, and metastasis of prostate cancer. Therefore, targeted therapy of prostate cancer stem cells may be effective for the treatment of prostate cancer. Prostate cancer stem cell markers must be identified to facilitate studies on prostate cancer radical treatment schemes, especially the specific markers of this disease. Previous research on prostate cancer stem cell markers mainly focus on CD44 and CD133. Along with in-depth studies, a substantial number of new markers have been found with research development. This review summarizes the widely studied and recently discovered markers found in the field of prostate cancer stem cells.

Objective To explore the level changes of serum hypersensitive C-reactive protein (hs-CRP) in patients with cardiac syndrome X (CSX) and the effect of atorvastatin on hs-CRP level. Methods 25 patients with CSX and 10 matched healthy subjects were enrolled in this study, and the serum hs-CRP levels were compared between the patients and healthy subjects. The patients were randomly devidid into routine therapy group (n=12), which received diltiazem 30mg t.i.d and isorbide dinitrate 10mg t.i.d, and atovastatin thrapy group (n=13), which received the same dosage of diltiazem and isobide dinitrate, and atovastatin 20mg/d additionally. The course of treatment was 12 weeks. At the beginning and the end of study, standardized angina questionnaires and exercise stress test were performed in all patients, and their serum hs-CRP levels were measured. Results ⑴The serum hs-CRP levels of patients with CSX were significantly hagher than those of healthy subjects(3 7?3 2mg/L vs 0 9?0 5mg/L, P

We used near-infrared spectroscopy technology to monitor and assess the treatment effect of dehydrating agent in injured rat brain in real time style. We employed the brain edema model in rats resulting from Feeney' s freefall damage, then treated with different doses of mannitol, and collected reduced scattering coefficient (p',) and intracranial pressure (ICP) values after the injury and during the treatment. The results showed that brain edema happened 1 h after the injury in rats' brain tissue, peaked around 72 h after injury, and then began to decrease gradually. The reduced scattering coefficient and ICP values of the treatment group injected with mannitol all decreased after administration. Compared with the effect of low-dose mannitol treatment, that of high-dose mannitol treatment was much better. The duration of the plateau was longer and most experiments results declined significantly. From this we conclude that the reduced scattering coefficient and ICP are consistent with the trend changes, and the reduced scattering coefficient could be used as an indicator for monitoring cerebral edema.
Animals ; Brain Edema ; diagnosis ; Brain Injuries ; therapy ; Dehydration ; Diuretics, Osmotic ; therapeutic use ; Intracranial Pressure ; Male ; Mannitol ; therapeutic use ; Monitoring, Physiologic ; Rats ; Spectroscopy, Near-Infrared

BACKGROUND:In clinical practice,there are some patients with labial inclined anterior teeth which influence their appearance,but they refuse orthodontic treatment because of many factors such as long-term treatment.Alternatively,these patients can receive aesthetics restoration with re-directional post-core and crown.OBJECTIVE:To investigate the influence of three kinds of post-core materials on fracture resistant ability of labial inclined maxillary central incisor with different changed angles.METHODS:Seventy-five maxillary central incisors were randomly divided into three groups:group Ⅰ was restored with Co-Cr post-core and porcelain-fused-to-metal (PFM) crown,group Ⅱ was restored with 50％ gold alloy post-core and PFM crown,group Ⅲ was restored with everStick fiber post and composite resin core and PFM crowns.Each group then was divided into five subgroups based on angles between the post-core and long axis of the tooth (0°,10°,20°,30°,40°).All specimens were embedded in acrylic resin and fixed in electronic universal testing machine.Force was loaded to each specimen until fracture occurred,and the maximum load values of fracture and the fracture mode were recorded.RESULTS AND CONCLUSION:The fracture resistance of group Ⅲ was superior to groups Ⅰ and Ⅱ (P ＜ 0.05),but there was no significant difference between group Ⅰ and group Ⅱ (P ＞ 0.05).The maximum load value of the three groups decreased as the changed angle increased.The fracture of group Ⅲ mostly appeared on the 1/3 of the root neck,while the fracture of groups Ⅰ and Ⅱ mostly appeared on the 1/3 of root central or the 1/3 of the root neck.So,the fracture resistance and fracture mode of central incisor under changed angles restored with everStick fiber post were superior to that with cast metal post-core.During re-directional restoration of labial inclined maxillary anterior teeth,everStick fiber post is recommended as a better choice to reduce the risk of catastrophic fractures.Also it suggests that excessive changed angle should be avoided to prevent root fracture.

AIM: To establish the quality standard for Jinqiaomai Tablets ( Fagopyri Dibotryis Rhizoma). METHODS: Fagopyri Dibotryis Rhizoma was identified by TLC. The contents of (-)-epicatechin and procyanidin B2 were determined simultaneously by HPLC,the Symmetry C18 column (5 ?m,3. 9 mm id ? 150 mm) was used. The water (adjust pH value to 3. 00 ? 0. 02 with phosphoric acid)-acetonitrile (92 ∶ 8) was used as a mobile phase,the detecting wavelength was at 280 nm. The column temperature was at 35 ℃. RESULTS: Fagopyri Dibotryis Rhizoma could be identified. The calibration curve of (-)-epicatechin was linear in the range of 0. 113 6-2. 272 ?g with the correlation of 0. 999 968 (n = 8). The average recovery of (-)-epicatechin was 97. 12% ,RSD = 0. 92% (n = 6). The calibration curve of procyanidin B2 was linear in the range of 0. 166 4-1. 664 ?g with the correlation of 0. 999 702(n = 8). The average recovery of procyanidin B2 was 98. 26% ,RSD = 0. 61% (n = 6). CONCLUSION: The method is specific,reliable and accurate,so it can be used for the quality control of Jinqiaomai Tablets.

Objective To observe the intervention effects of fluid wax on the therapeutic course of patients with adhesive small bowel obstruction.Methods Two hundreds and eighty-eight patients with adhesive small bowel obstruction admitted into the Department of Gastrointestinal Surgery of Huzhou Central Hospital from December 2014 to June 2016 were enrolled, and they were divided into a fluid wax group and acontrol group by mechanical sampling method, each group 144 cases. The control group was treated with conventional comprehensive non-surgical treatment, in the fluid wax group, on the basis of the above conventional treatment, additionally after 2 hours of gastrointestinal decompression, the fluid wax 3 mL/kg was injected through a gastric tube that then was closed by a clip for 2 hours. The first exhaust and defecation times, the time for amelioration of abdominal pain, the time of gas-liquid flat disappearance, the length of stay in hospital, the rate of operation and the occurrence of adverse reactions were observed in the two groups.Results After treatment, the first exhaust time, the first defecation time, the time of relieving abdominal pain, the time of gas-liquid flat disappearance and the length of stay in hospital were significantly shorter in fluid wax group than those in control group [the first exhaust time (hours): 29.97±19.71 vs. 49.28±33.61, the first defecation time (hours): 60.25±28.37 vs.74.23±50.12, the time of relieving abdominal pain (hours): 35.78±20.98 vs. 51.83±25.02, the time of gas-liquid flat disappearance (hours): 71.60±39.50 vs. 90.98±57.91, the length of stay in hospital (days): 7.00±3.77 vs. 9.00±5.81, allP < 0.05], and the rate of operation in the fluid wax group was lower than that in the control group [18.75% (27/144) vs. 27.08% (39/144),P < 0.05]. No patients died in the two groups. In nearly 1 year follow-up, there were no adverse reactions associated with the study in the fluid wax group.Conclusion The intervention of fluid wax combined with conventional non-surgical methods can significantly shorten the disease course, reduce the rate of operation and the hospitalization time in patients with adhesive small bowel obstruction.

Objective To evaluate the safety and efficacy of percutaneous closure for muscular ventricular septal defect(MVSD).Methods Fifty-one patients diagnosed as MVSD from October 2011 to July 2016 at Guangdong General Hospital were enrolled including 32 males and 19 females,ranging from 1 to 16 (5.12 ±3.52) years in age,weight (20.19 ± 10.55) kg.The diameter of the MVSD was (4.82 ± 2.51) mm which was measured by transthoracic echocardiography (TTE),and multiple defects were found in 10 patients.The choice of plugging device and transport system depended on the size,position and status of MVSD.TTE and left ventricular (LV) angiography were performed before and after release of the device to evaluate the therapeutic effects.Electrocardiogram and TTE were performed during follow-up period at 24 h,1 month,3 months,6 months and 12 months after operation and yearly thereafter.Results Eight cases showed no hemodynamic significance through standard catheter examination then the interventional therapy was stopped.Cardiac arrest was found in 1 case when the long sheath was transported along the track,and the procedure was terminated immediately,and the selective surgical operation was performed after successful rescue.The devices were successfully placed in the rest of 42 patients (97.6％) with operation time (90.68 ± 36.42) min and fluoroscopy time (18.67 ± 10.89) min.The average of follow-up time was (13.82 ± 13.84) months ranging from 1 to 48 months.It was found that mild residual shunts showed in 4 cases during operation,mild tricuspid regurgitation showed in 2 cases and trivial aortic regurgitation showed in 1 case at 6 months after operation,but there was no need to intervene anymore.Conclusions Percutaneous closure of MVSD in children is safe and effective with high successful rate and low incidence of complication.The middle-term follow-up findings were satisfactory.

Objective To evaluate the recent clinical efficacy and safety of recombinant human adenovirus-p53 (rAd-p53) combined with radiochemotherapy in treatment for cervical squamocellular cancer (Sqc).Methods 21 patients with cervical squamous cell carcinoma were randomly divided into 2 groups:experiment group (10 patients received treatment with rAd-p53 intratumor injection combined with radiochemotherapy) and control group (12 patients treated with radiochemotherapy alone).The recent efficacy of the two groups were compared at the end of treatment,and major drug toxicity between the two group were compared during the treatment.Results In treatment group,2 cases recieved CR,6 cases PR,2 cases SD,and the rate of total effective was 80.0 ％.In control group,3 cases recieved CR,6 cases SD,2 cases PD,and the rate of total effective was 27.3 ％.The rate of total effective in treatment group is superior to that in control group (x2 =76.00,P ＜ 0.05).The major reaction of rAd-p53 were transient fever after rAd-p53 administration.The two groups of bone marrow inhibition reaction rate with no statistical difference (x2 =119.50,P ＞ 0.05).Conclusion rAd-p53 combined with radiochemotherapy in treatment of advanced cervical squamocellular carcinoma has a synergistic effect.It is a safe,effective and convenient treatment method for rAd-p53 intratumor injection.