Traditional medical education has made enormous contribution for human health care and medical work for a long time. Since entering new century, the structure of order in medical and health work is confronted with severe challenges. Traditional medical education is severiously deficient in educational theory,humanism in guiding principle and practice and environment.These phenomena are, we think, the inevidable reflection of the inner conflicts between traditional Chinese medicine and Western medicine, the consequences resulting mainly from the separation of science and technology rom humanism in the technological era,and the biproducts from the unfavourable influence in the couse of marketingation. The outlet which changes the current situation lies in probing the medical educational pattern in the new century, doing the construction that the humane spirit of the medical education dimension well.

By analyzing the status quo of moral construction of current Chinese medical students,the authors make an in-depth analysis and accurate grasp of human nature,establish a scientific concept of morality education,and clarify the objective which aims to develop students into real "socialized individuals" with mental health,personal integrity and all-round development.Four moral methods including vertical process,horizontal extension,virtual network and class building are created to promote the ideological and moral construction for medical students.

Objective To explore the influence of contrast agent injection rate and sequence time resolution on the dynamic contrast-enhanced MR iamging of brain glioma.Methods Fifty eight cases with suspected cerebral glioma were scanned on a Verio 3.0T MRI with the regular head sequences and DCE sequences.All the cases were divided into two groups.There were 41 cases in the regular group in which the injection rate was 3 mL/s and the time resolution was above 5.36 s.Another 1 7 cases were in the optimal group in which the injection rate was 4 mL/s and the time resolution was about 4 s.The DCE images of two groups were Postprocessed by the Tofts model analysis.The curve shape of AIF were evaluated and divided in to the excellent or the secondary level.Chi-square test was used to compare the results of two groups.Results The curve shape of optimal group was much better than that of regular group(P<0.05).Conclusion Speeding up the injection rate and the time resolution of DCE sequences would improve the image quality of DCE MRI of glioma.

With the development of gene technology,it is gradually possible and much better for genetic modification of bone marrow stem cells in treatment of hindlimb ischemia. Gene modification of cells prior to their transplantation, especially stem cells, enhances their survival and increases their function in cell therapy. But this treatment is only used for the experimental stage. Here, we review the current uses of gene-modified bone marrow stem cells in vascular disease.

Objective To analyze the electrocardiogram (ECG) characteristics of apical ballooning syndrome (ABS) in female patients in China. Methods 50 female cases of ABS patients from medical periodicals in China were analyzed. Results 50 patients were in sinus rhythm, 98% patients were abnormal in ECG. ST segment elevated in 85.7% of them, ranged 0.1-0.8 mV, and came down in 30 min-2 weeks. T wave inversion was found in 42% of them, 24% with pathological Q wave, which disappeared in 6 hours-1 month. 28% showed QT interval prolongated, 38% complicated with arrhythmia. Conclusion The abnormal ECG is common in female patients with ABS, which is transient and reversible.

N-methyl-D-aspartate (NMDA) receptors are a group of ionotropic glutamate receptors, which are in close contact with the synaptic plasticity, long-term potentiation (LTP) and long-term depression (LTD) in the cerebral cortex or the hippocampus. This article reviewed the recent development in this field.

Breast cancer is a malignant tumor from normal breast epithelial. In recent years, many literature reports sought to determine the expression of predicted target genes of microRNA and their potential function, pathways and networks, which are involved in the tumorigenesis, metastasis and prognosis of breast cancer. The miR-21 has recently been found to be highly expressed in solid tumors than normal tissue, and it has exposed some layers of gene expression regulation that becomes a hot topic of breast cancer. This paper briefly reviews advances in research on miR-21 in breast cancer.
Breast Neoplasms ; Female ; Humans ; MicroRNAs

OBJECTIVETo investigate the changes on the number and distribution of myoepithelial cells (MECs) during parotid gland regeneration.

METHODSA total of 54 Wistar rats were divided into eight experimental groups and one normal control group, with six rats in each group. The right parotid ducts of the rats in the experimental groups were ligated for 14 days and then reopened. The parotid tissue specimens were harvested at days 0, 1, 3, 5, 7, 10, 14, and 21. The histological changes of the regenerating gland were examined using hematoxylin-eosin staining. Immunohistochemical labeling was also performed to investigate the changes in the number and distribution of MECs at different time points of parotid regeneration. Both the histological and immunohistochemical changes observed in the experimental groups were compared with those in the normal control group.

RESULTSThe parotid gland showed marked atrophy 14 days after ligation. Most acinar cells disap- peared, but the number of duct-like structures obviously increased. MECs apparently increased in number and were mainly located at the periphery of the duct-like structures. Three days after duct reopening, the number of acinar cells significantly increased and the duct-like structures significantly reduced. Meanwhile, MECs also decreased in number and were mainly located at the periphery of the newly formed acini and duct-like structures. The number of MECs noticeably decreased 3 and 5 days after duct reopening. At 14 days after duct reopening, the glandular structures and the number and distribution of MECs returned to normal compared with those in the normal control group.

CONCLUSIONThe number and distribution of MECs returned to normal condition after parotid gland atrophy. Parotid regeneration mainly occurred within 5 days after duct reopening.

Objective:To investigate the histological changes of parotid gland after ligation and reopening of the main duct.Meth-ods:The right parotid main duct was ligated for 14 days and then reopened,the specimen were harvested at day 0,1,3,5,7,10,14 and 21 after reopening(n=5 )respectively.By HE staining and Morphology measurement,the morphological and volume fraction changes of acinus,duct and mesenchyma were observed and measured.Results:The parotid gland atrophied significantly 14 days af-ter the duct ligation.From day 3 after duct reopening,the acinar volume fraction continued to increase,both the duct and the mesen-chymal tissue volume fraction continued to decrease significantly(P<0.05).After 14 days of duct reopening,there was no signifi-cant difference in volume fraction of acinar,duct and mesenchyma,compared with those of normal control group(P>0.05).Conclu-sion:After reopening of the ligated main duct the atrophied parotid gland is able to regenerate to be normal.

To examine the deletion and point mutation of WWOX (WW domain containing oxidoreductase) exons 6-8 in human non-small cell lung cancer and their possible relationship with pathological stages, tumor tissues and the corresponding normal tissues were obtained from 44 Chinese patients who had undergone surgery for non-small cell lung cancer. RNA was extracted from each sample and deletion and mutation of WWOX exons 6-8 were analyzed by RT-PCR and DNA sequencing. Our results showed that 28 of 44 (63.6%) lung cancer samples showed loss of WWOX exons 6-8 transcript and the deletion was detected in only 3 of 44 (6.8%) corresponding adjacent normal tissues (P < 0.05). The transcript sequencing analyses of the 16 lung cancer samples without transcript loss of WWOX exons 6-8 revealed no difference from the sequence of GenBank. Moreover, the deletion of WWOX exons 6-8 was significantly higher in the smokers when compared with the non-smokers. It is also higher in the men and squamous carcinomas than in women and adenocarcinomas (P < 0. 05). The deletion, however, was not found to be associated with pathological stages of the tumors. Our study documented a high incidence of deletion of WWOX exons 6-8 in non-small cell lung cancer in Chinese patients and suggested that the frequent loss of WWOX exons 6-8 might play an important role in the tumorigenesis of non-small cell lung cancer in Chinese. WWOX exons 6-8 may serves as a candidate molecular target of smoking carcinogenesis, and point mutation is not a predominant way of alteration of WWOX exons 6-8.
Carcinoma, Non-Small-Cell Lung/*genetics ; Exons/genetics ; Gene Deletion ; Loss of Heterozygosity ; Lung Neoplasms/*genetics ; Oxidoreductases/*genetics ; Point Mutation ; Sequence Analysis, DNA ; Tumor Suppressor Proteins