Objective To investigate the expression levels and significance of interferon-γ(IFN-γ),interleukin (IL)-10 mRNA in peritoneal fluid mononuclear cells (PFMC) in patients with endometriosis (EM).Methods A total of 55 patients with EM diagnosed histopathologically after operation were enrolled as EM group,and 52 patients without EM were enrolled as control group.The PFMC were isolated,and the expression levels of IFN-γ and IL-10 mRNA were determined by TaqMan real-time reverse transcriptase-polymerase chain reaction (RT-PCR).Results It was found that IFN-γ mRNA expression level was significantly decreased in EM group compared with that in control group (P＜0.05),while IL-10 mRNA expression level was significantly increased in EM group compared with that in control group (P＜0.05).The ratio of IFN-γ/ IL-10 was significantly lower in EM group than that in control group (P＜0.05).The ratio of IFN-γIL-10 was significantly higher in patients with stage Ⅰ-Ⅱ than that in patients with stage Ⅲ-Ⅳ (P＜0.05).Conclusion The imbalance of IFN-γ/ IL-10 in PFMC is associated with the pathogenesis of EM.

Objective To investigate the employment pressure among graduates from nursing school,and explore influencing factors,in order to make suggestions on education management,career guidance and psychological intervention.Methods Students graduated from Nanyang Medical College in 2012 who majored in nursing and nursing graduates from Nanyang Institute of Technology were surveyed with medical students employment pressure questionnaire designed by DU Tian-jiao from China Medical University.SPSS13.0 was used to establish database and analyze data.Results The total employment pressure of all students was M=3.2.Seven stressors scores from high to level were:post requirements,social environment,family support,personal qualities,the school effect,professional requirements,and career guidance.Multiple regression analysis showed that whether or not the student leaders,different sources and the education background were the influencing factors of employment pressure.Conclusions This research suggests that different psychological intervention should be given to students majored in different specialty and sources in order to release employment pressure and anxiety.

Objective Endovascular tubular stent grafting has emerged as an alternative therapeutic modality for patients with aortic dissections. However, its application has been limited to the descending thoracic aorta distal to the origin of the left subclavian artery. The initial clinical experience of endovascular branched stent graft repair for aortic arch dissections involving the left subclavian artery was reported. Methods From February 2004 to January 2005, the endovascular branched aortic stent-grafts made by Beijing YuHengJia SciTech Co. were applied in 42 patients with Stanford type B aortic dissection with the entry tears just beyond (

Objective To investigate the effects of a-melanocyte stimulating hormone (?MSH) on serum cytokine levels in rats with acute respiratory distress syndrome (ARDS) induced by acute hemorrhagic shock and intratracheal lipopolysaccharide (LPS) administration (two-hit model) .Methods Thirty male SD rats weighing (337? 25) g were randomly assigned to six groups of five animals in each group: group A normal control; group B ARDS control; group C-F treatment groups in which ?MSH 1.7 mg/kg was given at different time points - Ih before LPS(C), at the time as LPS was administratered (D), 1h after LPS (E) or together with and 3 h and 6 h after LPS (F) . The animals were anesthetized with intravenous thiopental 30 mg?kg-1 and tracheotomized and mechanically ventilated (FiO2 : 0.5, RR 100 bpm, VT12ml, I: E = 1:1.5) . Right common carotid artery was cannulated for BP monitoring, removal of blood and blood sampling. Acute hemorrhagic shock was induced by removal of blood and MAP was maintained at 45 mm Hg for 1 h, then the animals were resuscitated with reinfusion of removed blood and lactated Ringer's solution, then endotoxin (LPS 200 ?g/kg in 500 ?l normal saline). The criterion for ARDS was PaO2/FiO2

From pregnant women's psychological point of view, the variant characters and the underlying causes are analyzed when they are in the period of childbirth and puerperium. The role of medical staff and the treatments in clinical psychological heath for pregnant women are discussed, which could help the pregnant women give birth smoothly and healthy in puerperium.

Objective To investigate the clinicopathological characteristics of papillary thyroid carcinoma (PTC), and analyze their relationship with lymph node metastasis (LNM). Methods The clinicopathological data of 914 cases of PTC from January 2012 to December 2016 was analyzed retrospectively byχ2 test andχ2 trend test, including age, gender, tumor size, the number of lesions and LNM, in order to illuminate the characteristics of incidence and variation trend of PTC. Results The numbers of PTC cases in Shanxi Dayi Hospital increased year by year in recent 5 years (χ2=64.009, P=0.001). The rate of LNM was higher in age of ≤40 years old [34.9 % (84/241)] and > 70 years old [44.0 % (11/25)] than age 41-70 years old [15.6 % (101/648)] (χ2 = 39.577 and 14.009, both P= 0.000). It showed a triphasic pattern, which presented a uptrend with age decreasing in patients ≤ 40 years old (χ2= 10.490, P= 0.010), on the contrary, it appeared a rising trend with age increasing in patients>40 years old (χ2=10.170, P=0.010). The sex ratio of male and female was 1:2.97. The rate of LNM for male was higher than female (χ2=5.845, P=0.013). There was approximate risk of LNM between tumor diameter ≤1 cm and>1 cm (χ2=0.070, P=0.610). The rate of LNM in the cases of multiple lesions was apparently higher than that in cases of single lesions (χ2=145.440, P=0.000). Conclusions The incidence of PTC is on the rise. The risk factors of LNM are age (≤40 years old and>70 years old), male and multiple lesions.

BACKGROUND:Currently, human umbilical cord derived-mesenchymal stem cel s are mainly for local transplantation, which has some shortcomings, such as large trauma, bleeding, complications, that limit its widespread application in clinical practice. OBJECTIVE:To investigate the feasibility of intravenous transplantation of human umbilical cord derived-mesenchymal stem cel s for repair of spinal cord injury. METHODS:Eighty Wistar rats with spinal cord hitting were divided into five groups:blank control group with no transplantation (n=10), DMEM local transplantation group (n=15), DMEM intravenous transplantation group (n=15), cel local transplantation group (n=20), cel intravenous transplantation group (n=20). The functional recovery of spinal cord injury was observed with Basso, Beattie and Bresnahan scores at regular time as wel as hematoxylin-eosin staining and immunohistochemistry staining. RESULTS AND CONCLUSION:During 1 day to 2 weeks after transplantation, there was no significant difference in the Basso, Beattie and Bresnahan scores between the five groups;within 4-12 weeks after transplantation, the Basso, Beattie and Bresnahan scores were significantly higher in the two cel transplantation groups than the other three groups, but there was no difference between these two cel transplantation groups (P>0.05). Histological observation showed that the number of voids and glial scars was less in the cel local transplantation group and cel intravenous transplantation group compared with the other three groups, and there was also no difference between the two cel transplantation groups. These results indicate that the intravenous transplantation of human umbilical cord derived-mesenchymal stem cel s is similar to the local transplantation in the repair of acute spinal cord injury, which is simple and avoids secondary injuries and various complications. It is recommended that this method provide a new approach for cel transplantation.

Objective To evaluate the feasibility and significance of CT image-based threedimensional (3D) brachytherapy for cervical cancer.Methods Three-dimensional (3D) plan and twodimensional (2D) plan were designed for 55 CT images of brachytherapy from 12 cervical cancer patients who received radical radiotherapy in 2013.Dosimetric comparison was performed between the 3D plan and 2D plan,and paired t-test,Wilcoxon signed rank test,Pearson correlation analysis,and Spearman correlation analysis were performed.Results A point dose,D90,V100,CI,and CI' in 3D plan were higher than those in 2D plan (P=0.015,0.016,0.000,0.000,0.000).Bladder point dose,rectal point dose,and rectal D2 cm3 in 3D plan were slightly higher than those in 2D plan,but hot spot dose was significantly reduced in 3D plan (P =0.140,0.123,0.214).Bladder D2cm3 was significantly higher than bladder point dose (P =0.000).Sigmoid colon D2cm3 was more correlated with the average doses of the three highest rectal points than rectal D2 cm3 (r =0.314,0.630,P =0.000,0.000).V100 showed a linear relationship with high-risk CTV (r =0.981,P =0.000).Bladder D2cm3 was higher than 430 cGy when the bladder volume was more than 80 cm3 ;small intestinal D2 cm3 did not change significantly when the bladder volume was less than 115 cm3,but decreased significantly once the volume exceeded the value.Conclusions Compared with the traditional 2D plan,the 3D plan for CT image-based cervical cancer brachytherapy significantly increases the target coverage and conformity index,but does not significantly increase the doses to organs at risk.Point dose evaluation is confirmed to be inaccurate.The doses to the bladder,rectum,and small intestine can be adjusted by controlling the bladder volume.

Objective:To investigate the roles and regulation mechanism of miR-31 in human cutaneous squamous cell carcino-ma (cSCC) growth. Methods:cSCC cells were transfected with the antisense oligonucleotide (ASO) of miR-31, and the cSCC growth was tested by colony formation and in vivo tumor formation assays. The target gene of miR-31 was validated by Western blot and green fluorescent protein (GFP) reporter assay. The cells were then transfected with the siRNA of the target gene, and the effect of the target gene on cell growth was preformed by colony formation assay. Finally, real-time PCR and immunohistochemistry were used for analy-sis of the expression of miR-31 and its target gene. Results:miR-31 ASO resulted in a low number of cell colonies and small tumor vol-ume (P<0.05). Western blot showed that the cells with miR-31 ASO had a higher protein level of large tumor suppressor homolog 2 (LATS2) than the control. The 3' UTR of LATS2 had a binding site with miR-31, and miR-31 ASO increased the GFP intensity con-trolled by LATS2 3' UTR, whereas no effect was observed on the mutant LATS2 3' UTR. Western blot showed that LATS2 siRNA inhib-ited the expression of LATS2 protein by about 80%. Knocking down of LATS2 increased the colony number by about 70%or 1.3-fold in cSCC cells. Real-time PCR showed that miR-31 was overexpressed in most cSCC tissues, compared with normal tissues. An inverse relationship existed between miR-31 and LATS2 expression levels. Immunohistochemistry validated that LATS2 was downregulated in cSCC tissues. Conclusion:miR-31, which functions as an oncogene, promotes cSCC growth by suppressing LATS2 expression. Our da-ta suggest that miR-31 is a potential miRNA-based therapeutic target for cSCC growth.

Objective: To study the main pharmacodynamics of Zengguang Tablets. Methods: Mice were given Zengguang Tablets orally in dosages of 0.75g/kg and 1.50 g/kg. The celiac macrophage phagocytic function and antibody formation of serum hemolysin (IgM) of mice were determined. The mouse blood vacuity model was established by injecting cyclophosphamide intraperitoneally in the dosage of 80mg/kg. Results: Zengguang Tablets could raise the celiac macrophage phagocytic function of mice and promote the antibody formation of serum hemolysin. Conclusion: Zengguang Tablets has the enhancement on organism immune function.