[Objective] To explore the characteristics and achievements of YU Chang'sYuyi Grassin treating typhoid disease. [Method] Based on studying, thinking and analysing theYuyi Grass, combined with related literature research, it summed up the YU Chang characteristics and achievements in treating typhoid disease. [Results] YU Chang treating typhoid fever syndrome had accuracy differentiation, forcefully rectifying children with typhoid fever being mistaken for the fallacies of present concern of charlotte, fine solution of typhoid rehabilitation treatment; slow treatment for typhoid critical patients, illustrating the appropriate use of ginseng theory for typhoid fever, having the original opinion in such use. [Conclusion] The Yuyi Grass treating typhoid disease has rich experience and unique achievements. These insights and achievement in learning by using the theory of Treatise on Febrile Disease for treating typhoid disease have important guiding significance in Chinese medicine clinic.

The 6.5kb specific fragment containing the T7 promoter and the transcription vector was cut down from the full-length cDNA clone of Newcastle disease virus strain ZJI of goose origin,and thereafter it was self-ligated to form the high guality plasmid for mutagenesis.Site-directed mutagenesis technique was used for inserting three additional G nucleotides(nts) into the region between the T7 promter and the leader sequence of the NDV.The RT-PCR was employed to amplify the F/HN genes fragements,and then they were ligated by the shairing restriction enzyme BsmBI and finally the corresponding fragment in the mutatant full-length cDNA was substituted by the new one.The sequencing results showed that the three additional G nts were successfully inserted and the mutant nts in the full-length cDNA were corrected and all these studies lay a foundation for the research on the reverse genetics of NDV strain ZJI.

Objective To study the related factors of operating on Pilon bone fracture.Methods 35 cases with tibial Pilon fracture treated operative,according to the Reedi-Allgower were divided the type,Ⅱ type 15 examples,Ⅲ type 20 example.14 cases were treated with open redcution and inter fixation plate and screws and 11 cases were treated with open reduced minimal and exter fixation that cast.Results According to Mazur's grade system treatment was evaluated may in 28 cases,18 cases of which were type Ⅱ racture and 10 cases type Ⅲ fracture poor in 7 cases,2 cases of which were type Ⅱ fracture and 2 cases type Ⅲ fracture.Conclusion The curative effect with fracture type,repla-cement mass and operation time are relative in tibial Pilon fracture.

Kangjixiaoke Granules was composed Red Radix Ginseng,Rhizoma Coptidis,Radix Rehmannia and Rhizoma Polygonati Odorati.The experimental result showed that it pos- sessed obvious hypoglycemic action on normal mice,alloxan diabetes mice and hyper- glycemia mice induced by adrenaline.No toxic response and side-effect were observed in acute toxic test.

Usage of the fruit and bark of a Melia-family plant as a digestive tract-parasiticide and agricultural insecticide was recorded about two thousant years ago in ancient China. Toosendanin (TSN), a triterpenoid, is an effectual ingredient extracted from the plant. Studies have demonstrated that TSN selectively affects neurotransmitter release, effectively antagonizes botulism, induces cell differentiation and apoptosis and inhibits proliferation of various human cancer cells, inhibits feeding and dovelopment in insects and modifies K+- and Ca2+-channel activity. The research data to demonstrate that TSN inhibits K+-channel and facilitates L-type Ca2+-channel are summarized, and the mechanism of action of TSN is discussed.

Objective:To explore differences of graphic,phonological and semantic priming in Chinese character recognition between dyslexic children and normal children.Method:A priming paradigm was used to facilitate subjects to complete a lexical decision task.The differences of reaction time,priming effect and error rate between dyslexic children and normal children were analyzed.Results:The baseline adjusted facilitations of graphic,phonological and semantic priming in character recognition in dyslexic children were-0.010,-0.010,and 0.001 respectively.The dyslexic children even showed inhibition in graphic and phonological prime conditions.For the age control group the facilitations under the three prime conditions were 0.026,0.026 and 0.022.As for the reading level control group,results were 0.062,0.058 and 0.031 respectively.The differences of priming effects between dyslexic children and normal children were significant,especially in graphic and phonological priming conditions.The possible order of the three priming effects(from high to low)was:graphic,phonological and semantic.The interactions between reading ability and priming were not significant.Conclusion:Results from the present study suggest that there is a general deficit in graphic and phonological processing in character recognition in Chinese dyslexic children.This phenomenon can be interpreted by the connectionist model.

Objective To investigate the expression levels and significance of interferon-γ(IFN-γ),interleukin (IL)-10 mRNA in peritoneal fluid mononuclear cells (PFMC) in patients with endometriosis (EM).Methods A total of 55 patients with EM diagnosed histopathologically after operation were enrolled as EM group,and 52 patients without EM were enrolled as control group.The PFMC were isolated,and the expression levels of IFN-γ and IL-10 mRNA were determined by TaqMan real-time reverse transcriptase-polymerase chain reaction (RT-PCR).Results It was found that IFN-γ mRNA expression level was significantly decreased in EM group compared with that in control group (P＜0.05),while IL-10 mRNA expression level was significantly increased in EM group compared with that in control group (P＜0.05).The ratio of IFN-γ/ IL-10 was significantly lower in EM group than that in control group (P＜0.05).The ratio of IFN-γIL-10 was significantly higher in patients with stage Ⅰ-Ⅱ than that in patients with stage Ⅲ-Ⅳ (P＜0.05).Conclusion The imbalance of IFN-γ/ IL-10 in PFMC is associated with the pathogenesis of EM.

This paper presents plasma exchange(P.E) therapy in 8 cases of four types of immunic diseaes. The colony forming unit-T lymphocyte (TL-CFU), the symbol T-cell subsets with monoclone antibody (McAb), and the cisrculating immune complex (CIC)were examined before and after the therapy of P.E. and compared with the interelationship of each other. The experiments indicated that plasmapheresis may remove the CIC and noxious autoantibodies and regulation of the action of cellular immunity. All these functions are synthetic therapeutic effects. The indices of checkup on therapeutics were: TL-CFU, CIC and the ratio of T_H and T_S. After plasmaphersis, the ratio of CIC was lowered but the TL was higer than before (P

AIM:To investigate the effects of Ginkgo biloba extract 761(Egb761)on synaptophysin(SYN)expression in hippocampus of vascular dementia(VD)rats.METHODS:VD rat models,established by repeatedly cerebral ischemia/reperfusion,were randomly divided into two groups:model group and EGb761 treated group(both n=30),and another 30 condition-matched rats were selected as the sham-operated controls.Spatial learning and memory abilities of rats were assessed by Morris water maze(MWM)task,and SYN expression in hippocampal formation of rats in different groups was detected by immunohistochemical technique and image analysis.RESULTS:The MWM escape latency(EL)in model group was highly longer than that in the sham-operated group(P

AIM:To observe the dynamic changes of synapsin I expression and its phosphorylation in hippocampus in vascular dementia(VD)rats.METHODS:Eighty SD rats were randomly divided into sham-operated group(n=40)and VD model group(n=40),and the latter were established by repeatedly clipping the common carotid arteries with an intraperitoneal injection of sodium nitroprusside solution in anesthetized condition.The synaptic ultrastructural changes in hippocampal CA1 region and the expression levels of synapsinⅠ and phosphorylated synapsinⅠin hippocampus were observed by TEM and immunohistochemical staining method respectively in both sham-operated group and VD model group at 15 d,1 month,2 months and 4 months time points.RESULTS:No obviously pathological changes to CA1 area synapse were found in SO group.In model group rats,synaptic circa membrane ambiguity and fusion,synaptic circa membrane structure decreased the postsynaptic density,reduced synaptic vesicles and vesicle cluster.Above pathological changes became gradually severe along with the time prolongation after model-making operation.Compared with sham-operated group,the expression of synapsin I significantly reduced in CA1 region(P0.05)in model group was observed.The number of p-synapsin I positive neurons in DG and hippocampal CA1 region was less in model group than that in sham-operated group(P0.05).CONCLUSION:The damaged synaptic structure and depressed expression of synapsin I and its phosphorylation in presynaptic parts of hippocampus induced by repeatedly cerebral ischemia/reperfusion may contribute to the synaptic transmission disorders,especially the presynaptic disorder which may be one of the important pathogenesis of the initiation and development in vascular dementia rats.