Objective To study the effect of dexmedetomidine anesthesia on surgery condition, renin-angiotensin-aldosterone system (RAAS) activity and renal function of laparotomy patients.Methods 96 patients received laparotomy in our hospital during May 2012 to October 2015 were studied, which were divided into dexmedetomidine group (D group) and control group (C Group) by random number table method and received different anesthetic regimes.Then surgery condition, renal function indexes and plasma renin, angiotensin II levels of two groups were compared.Results The operation time, intraoperative blood loss, intraoperative transfusion volume and awakening time of D group and C group had no significant difference.10 min after opening abdomen and 10 min before closing abdomen, the plasma renin, angiotensin II, cystatin C (Cys-C),α1-microglobulin (α1-MG) contents were significantly lower than those of C group(P<0.05).Conclusion Dexmedetomidine anesthesia applicated in laparotomy could inhibit the RAAS activity, protect renal function, and its anesthesia effect is more precise.

Objective To investigate the effect of sufentanil midazolam intravenous anesthesia combined with epidural block on hemodynamics and postoperative consciousness after anesthesia.Methods 40 cases undergoing acute chest and abdominal surgery from January 2015 to December 2016 in Taizhou tumor hospital as the research objects, were randomly divided into the control group and the experimental group, 20 cases in each group.The control group were received sufentanil midazolam intravenous anesthesia, and the experimental group were received sufentanil midazolam intravenous anesthesia combined with epidural block.Hemodynamics and anesthesia consciousness in the two groups were compared.Results Compared with pre-induction, the diastolic and systolic blood pressure and the heart rate were significantly decreased at pre-intubation (P<0.05).There were no significant differences between the two groups at other observation time points.The intravenous anesthesia medication in the experimental group was significantly less than that in the control group, the difference was statistically significant (P<0.05).The recovery time in the experimental group was (31.5±4.2) min, the extubation time was (4.8±1.5) min, and the basic awake time was (8.2±2.4) min.The above data were significantly better than those in the control group (P<0.05).No complications such as postoperative agitation and respiratory depression occurred in the experimental group and the control group.Conclusion Sufentanil midazolam intravenous anesthesia combined with epidural block, hemodynamic stability, it can reduce the amount of anesthesia drugs and shorten the recovery time to some extent, and is worthy of clinical application.

Objective To study the effect of seven sevoflurane or propofol combined with remifentanil anesthesia on patients undergoing laparoscopic surgery.Methods In Taizhou Tumor Hospital from January 2015 to December 2016 were 100 cases of patients with laparoscopic surgery as the research object in the course of the study, were randomly divided into control group and experimental group two were 50 cases each.Patients in the control group were treated with propofol and remifentanil anesthesia.The experimental group was given seven sevoflurane and remifentanil anesthesia.Comparative analysis of the experimental group and the control group of patients with anesthesia effect.Results After the corresponding anesthesia, patients in the experimental group extubation time, recovery time was significantly better than the control group, with statistical difference(P<0.05).In the experimental group, 15 minutes after extubation, the Steward recovery score was(5.04±0.65), while the control group score was(4.12±0.54).Can be obtained, the quality of patients' recovery was significantly higher than that of the control group, with statistical difference(P<0.05).The cognitive function scores of the experimental group were significantly higher than those of the control group after extubation(2 h), and the difference was statistically significant(P<0.05).Conclusion Compared with propofol remifentanil anesthesia, seven sevoflurane remifentanil anesthesia effect is good, can improve the patient's recovery quality, to help with the recovery of cognitive function, with further clinical promotion and application significance.

Objective To investigate and analyze the clinical observation of low dose sufentanil in the prevention of shivering and traction during cesarean section. Methods 100 cases of cesarean section in our hospital from January 2015 to December 2016 were selected and randomly divided into two groups, the control group and the experimental group, with 50 patients in each group. The control group received bupivacaine anesthesia, and the experimental group received sufentanil anesthesia on the basis of sufentanil. The clinical indexes of the experimental group and the control group were compared and analyzed. Results After the corresponding anesthesia, there was no moderate or severe traction pain in the experimental group and the control group, and 29 cases of mild traction pain in the control group, with a rate of 58%. There were 7 patients with mild traction pain in the experimental group, with a rate of 14%. The probability of mild traction pain in the experimental group was significantly lower than that in the control group, with statistical difference (P<0.05). The incidence of shivering in the experimental group was 16%, significantly lower than that in the control group, with a rate of 52%, which was statistically significant (P<0.05). The adverse reaction rate of the experimental group was 12%, significantly lower than that of the control group, the probability of occurrence was 42%, which was statistically significant (P<0.05). Conclusion The application of small dose of sufentanil in anesthesia in cesarean section can reduce the occurrence probability of visceral pain in a large extent, prevention of shivering, high safety, low incidence rate of adverse reaction, with further clinical promotion and application significance.

Objective To study the effect of different doses of dexmedetomidine combined with propofol and remifentanil in patients undergoing abdominal surgery. Methods 200 cases of abdominal operation in our hospital from January 2015 to August 2016 were selected as the subjects, and were randomly divided into the experimental group and the control group, with 100 patients in each group. Patients in the control group received 0.4μg/kg, dexmedetomidine hydrochloride injection combined with propofol and remifentanil anesthesia, and the experimental group received 0.8μg/kg, Dexmedetomidine Hydrochloride Injection anesthesia. The adverse reactions, extubation time and recovery time of the experimental group and the control group were compared and analyzed. Results After the corresponding dose of dexmedetomidine, the patients in the experimental group were 6 cases of nausea and vomiting, 7 patients with chills, 13 cases of adverse reactions, the total incidence was 13%. In the control group, shivering occurred in 23 cases, nausea and vomiting occurred in 12 patients, and the number of adverse reactions in 35 cases, the incidence of adverse reactions was 35%. The probability of adverse reactions in the control group was significantly higher than that in the control group, with statistical difference (P<0.05). The recovery time and extubation time of the experimental group were significantly lower than those of the control group, with statistical difference (P<0.05). Conclusion 0.8μg / kg dexmedetomidine hydrochloride Iinjection combined with propofol and remifentanil anesthesia in abdominal surgery is better anesthesia effect, can significantly shorten the time of patients with tube drawing, high safety, reduce the pain and risk of surgery patients has clinical significance in the further promotion and application.

Objective To compare the effect of sevoflurane and propofol in elderly surgery patients towards cardiac damage.Methods 45 elderly patients with hypertension were divided into Sevoflurane group and Propofol group,which were observed changes of their ECG ST-segment,Mb,CK-MB,cTnI.Results 24 hours after induction of anesthesia,cardiac damage could be observed;Compared with Sevoflurane group,levels of CK-MB,Mb and cTnI increased significantly 24 hours after induction of anesthesia(t =2.771,0.681,0.557,all P ＜ 0.05),ST-segment significantly depressed at the same time[(-1.96±0.06)mV,(-1.45±0.04)mV](P ＜0.05).Conclusion Elderly patients with hypertension show cardiac damage during pevi-operative stage;Compared with propofol,sevoflurane shows lighter damage to cardiac muscle.

Objective:To construct a specific small hairpin RNA (shRNA) expressing vectors against human receptor for advanced glycation end product (RAGE) gene and study its inhibitory effect on the proliferation of androgen-independent prostate cancer cells DU145. Methods:Four RAGE specific oligonucleotides were designed and synthesized. These oligonucleotides were annealed to forill double strand DNA fragments and this fragment was cloned into psi-U6 plasmid. The recombinants were transfected into RAGE-overexpressing sub DU145-2C1 cells. Cellular morphology and transfection efficiency were observed under fluorescence microscope. The inhibitory effect of RAGE shRNA construct on RAGE mRNA and protein expression was examined with semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting, respectively. The cellular proliferation was detected with cell counting kit-8 (CCK-8). Scratch test was used to observe the migration of DU145 cells.Results:RAGE shRNA expression plasmids were successfully constructed and transfected into sub DU145-2C1 cells. It can effectively inhibit the expression of RAGE mRNA (P<0.05). The inhibitory effects of shRNA RAGE-1 (R1) was the most stronger. The RAGE mRNA expression was inhibited by 84% and RAGE protein expression was inhibited by 27%. Compared with negative control, the proliferation potential was significantly decreased in shRNA RAGE-transfected cells. The cell migration capability had no significant changes. Conclusion:RAGE shRNA effectively inhibited the expression of RAGE mRNA and protein and suppressed the proliferation of DU145 cells in vitro.

Objective To evaluate the effects of community-based rehabilitation therapy on activitv of daily living performance of the Chinese stroke patients. Methods In a single blinded,randomized,controlled multicenter trial,737 consecutive stroke patients were stratified by two groups of cerebral infarction and hemorrhage. All the patients were randomly divided into a rehabilitation group and a control group.The patients in the rehabilitation group received atandardized community-based rehabilitation therapy,while those in the control group did not.All the patients were followed up for 5 months for evaluation of their performance with activity of daily living by using Modified Barthel Index before intervention,after follow-up 2 and 5 months,respectively. Results Patients in the rehabilitation group performed better in ADL as reflected by the Modified Barthel Index score than those in the control group after 5 months of intervention(P＜0.05).Although both the rehabilitation group and control group improved over time,the rehabilitation group showed a greater improvement with the Modified Barthel Index scores when compared with the control group(P＜0.01).After 5 months follow-up,the total gain of Modified Barthel Index scores was 26.28 and 32.89 for those with cerebral infarction and hemorrhage,respectively,in the rehabilitation group. In comparison,the gain of Modified Barthel Index scores was 7.65 and 2 1.70 for those with cerebral infarction and those with hemorrhage,respectively in the control group.This implies a difference in improvement of 1 8.63 in cerebral infarction group and 1 1.19 in hemorrhage group,in favor of the rehabilitation group. Conclusion Standardized community-based rehabilitation therapy may help stroke patients to improve their performance in activity of daily living significantly.