Objective:To investigate the correlation between MRI features of breast cancer and the expression of CBL-B, AXL and ITGB2, and further to provide new imaging ideas for the early diagnosis of breast cancer, the designation of treatment plan and the preoperative non-invasive assessment of breast cancer prognosis.Methods:204 female patients with breast cancer who visited Linyi Central Hospital from Sep. 2017 to Sep. 2020 and received surgical treatment were selected as research objects. A total of 204 lesions were selected. Scanning was performed using Philips All-digital Ingenia3.0T MRI. Tumor diameter, shape, edge, intensity pattern, time-signal intensity curve (TIC) type, early intensity rate and ADC value were recorded. Immunohistochemical SP method was used to detect the expression levels of CBL-B, AXL and ITGB2 in breast cancer tissues and adjacent tissues.Results:The proportion of patients with low expression of CBL-B, high expression of AXL and high expression of ITGB2 in breast cancer tissues was significantly higher than that in adjacent tissues (All P<0.05) . Univariate analysis showed that tumor diameter, shape, early enhancement rate and ADC value in MRI features were correlated with the expression of CBL-B protein in breast cancer (All P<0.05) . The tumor diameter, shape and TIC typing in MRI features were associated with AXL protein expression in breast cancer. The tumor diameter, TIC typing, early enhancement rate and ADC value in MRI features were related to ITGB2 protein expression. Binary Logistic regression analysis showed that tumor diameter ≥3 cm and early enhancement rate ≥100% were independent risk factors for low expression of CBL-B in breast cancer ( P<0.05) . Tumor diameter ≥3 cm, and irregular shape or burr were independent risk factors for AXL high expression ( P<0.05) . Tumor diameter ≥3 cm, early enhancement rate ≥100%, ADC value ≥1.12×10 -3 mm 2/s were independent risk factors for high ITGB2 expression in breast cancer ( P<0.05) . Conclusion:MRI features of breast cancer can be used to predict the expression of CBL-B, AXL, and ITGB2, so as to provide reference for clinical diagnosis, treatment and prognosis assessment of the disease.

In the treatment of 48 cases of peripheral facial paralysis by puncturing Yangbai (GB 14)toward Yuyao (Ex-HN 4), Dicang (ST 4) toward Jiache (ST 6), Xiaguan (ST 7) toward Jiache (ST 6),Sibai (ST 2) toward Quanliao (SI 18), Baihui (GV 20) toward Taiyang (Ex-HN 5), Yintang (Ex-HN 3)toward Shangen (Ex-HN), Cuanzhu (BL 2) toward Jingming (BL 1), and Hegu (LI 4) toward Laogong (PC 8) on the sick side, with other acupoints added in accordance with the symptoms, the results showed cure in 39 cases, remarkable effect in 7 cases, failure in 2 cases, and the curative rate in 81.2% and the effective rate in 95.8%.

Objective:To explore the diagnostic performance of ultrasound attenuation imaging (ATI) in grading the degree of hepatic steatosis in metabolic dysfunction-associated fatty liver disease (MAFLD).Methods:The liver gray-scale ultrasound and ATI examinations were performed on 212 subjects who were treated in Zhongshan Hospital Affiliated to Fudan University from August 2020 to March 2021. The attenuation coefficient(AC) values among different degrees of hepatic steatosis were analyzed and the diagnostic performance of ATI was evaluated. Relationships between AC values and clinical characteristics were assessed by Pearson′s correlation analysis.Results:The AC values for normal liver, mild, moderate and severe fatty liver were (0.56±0.05)dB·cm -1·MHz -1, (0.68±0.09)dB·cm -1·MHz -1, (0.82±0.09)dB·cm -1·MHz -1, (0.94±0.09)dB·cm -1·MHz -1, respectively. There were significant differences in AC values among different hepatic steatosis divisions( P<0.008). There was highly significant correlation between AC values and the degree of hepatic steatosis( r=0.860, P<0.01), moderate correlation between AC values and BMI( r=0.425, P<0.01), weak correlation between AC values and HDL-C( r=-0.237, P=0.029), no correlations between AC values and age, TC, TG, LDL-C ( r=0.083, 0.055, 0.133, -0.039, all P>0.05) .The areas under the receiver operating characteristics curve of ATI for mild fatty liver and above, moderate fatty liver and above, severe fatty liver and above were 0.958, 0.962, 0.918; the sensitivity were 90.1%, 95.8%, 94.9%, the specificity were 96.1%, 87.1%, 73.9%, and the cut-off values were 0.666 dB·cm -1·MHz -1, 0.719 dB·cm -1·MHz -1, 0.803 dB·cm -1·MHz -1, respectively. Conclusions:ATI is a reliable and convenient method for evaluating the degree of hepatic steatosis in MAFLD.

Objective:To analyze the viscosity characteristics of liver tumors and investigate the clinical value of shear wave dispersion (SWD) in the differentiation of benign and malignant liver tumors.Methods:A total of 103 patients with focal liver lesions were prospectively collected in Zhongshan Hospital Affiliated to Fudan University from October 2020 to July 2021, including 80 cases with single lesion and 23 cases with multiple lesions, and only the largest lesion was observed in patients with multiple lesions. SWD values were measured within the tumor and in the liver parenchyma 2 cm away from the tumor, and were compared between benign tumor group and maligant tumor group. The ROC curves of SWD value, SWD ratio and their combination in differentiating benign and malignant liver tumors were plotted respectively, and the optimal diagnostic threshold, the sensitivity, specificity and accuracy of different diagnostic methods were analyzed.Results:Among the 103 patients, 35 were benign and 68 were malignant. The SWD value of liver benign tumor group was lower than that of liver malignant tumor group [(16.38±3.58)m·s -1·kHz -1 vs (18.59±3.12)m·s -1·kHz -1], the SWD value of liver parenchyma background in liver benign tumor group was lower than that in liver malignant tumor group [(10.88±3.37)m·s -1·kHz -1 vs (14.31±3.34)m·s -1·kHz -1], and the differences were statistically significant (all P<0.05). The SWD ratio of benign tumor to surrounding liver parenchyma was higher than that of malignant tumor group [1.57(1.25, 2.00) vs 1.27(1.06, 1.57)], and the difference was statistically significant ( P<0.05). When the SWD value >15.60 m·s -1·kHz -1 was used as the cut-off value, the area under ROC curve (AUC) was 0.72, the sensitivity was 88.2%, the specificity was 51.4%, and the accuracy was 75.7%. The sensitivity, specificity, accuracy and AUC were 58.8%, 74.2%, 63.1%, and 0.68, respectively, when the ratio of SWD value<1.32 was used as the cut-off value. SWD value combined with SWD ratio for the diagnosis of liver malignant tumor, the AUC was 0.88, the sensitivity was 82.3%, the specificity was 83.0%, and accuracy was 81.6%. The diagnostic efficacy of the two in combination for liver malignant tumor was superior to SWD value ( Z=2.678, P=0.007 4) and SWD value ratio ( Z=3.822, P=0.000 1). Conclusions:SWD imaging can reflect the viscosity information of liver tumors and surrounding liver parenchyma, and has potential clinical application value in the differentiation of benign and malignant tumors.

Objective To analyze the epidemic characteristics of influenza in children in Jinshan District of Shanghai，so as to provide scientific basis for prevention and control of influenza in children. Methods We analyzed the surveillance data as well as the results of virus isolation and identification in children with influenza-like illness （ILI） in Jinshan District of Shanghai from January 2010 to December 2019. Results There were two annual epidemic peaks in 2010， 2016， 2017 and 2018， one annual epidemic peak in winter and spring in 2011 and 2013， and two annual epidemic peaks in 2012， 2014， 2015 and 2019. A total of 3 859 ILI cases were collected in the past 10 years， and 848 positive virus strains were isolated， with a positive rate of 21.97%. Among them， 371 were seasonal H3N2， 163 were new H1N1， 314 were type B（among them， 226 were yamagata and 88 were victoria）. The positive rates of influenza virus in different children groups were 0.88% in scattered children， 6.76% in kindergarten children， and 14.33% in students， respectively （P= 0.001）. Conclusion From January 2010 to December 2019， seasonal H3N2 was the predominant virus strain in children influenza epidemic in Jinshan District of Shanghai. The influenza epidemic shows a strong seasonal feature with double peaks in winter/spring， summer or autumn. The positive rate of influenza virus in middle school students is the highest in different age groups， followed by kindergarten children.

Nowadays, orthodontic treatment has become increasingly popular. However, the biological mechanisms of orthodontic tooth movement (OTM) have not been fully elucidated. We were aiming to summarize the evidences regarding the mechanisms of OTM. Firstly, we introduced the research models as a basis for further discussion of mechanisms. Secondly, we proposed a new hypothesis regarding the primary roles of periodontal ligament cells (PDLCs) and osteocytes involved in OTM mechanisms and summarized the biomechanical and biological responses of the periodontium in OTM through four steps, basically in OTM temporal sequences, as follows: (1) Extracellular mechanobiology of periodontium: biological, mechanical, and material changes of acellular components in periodontium under orthodontic forces were introduced. (2) Cell strain: the sensing, transduction, and regulation of mechanical stimuli in PDLCs and osteocytes. (3) Cell activation and differentiation: the activation and differentiation mechanisms of osteoblast and osteoclast, the force-induced sterile inflammation, and the communication networks consisting of sensors and effectors. (4) Tissue remodeling: the remodeling of bone and periodontal ligament (PDL) in the compression side and tension side responding to mechanical stimuli and root resorption. Lastly, we talked about the clinical implications of the updated OTM mechanisms, regarding optimal orthodontic force (OOF), acceleration of OTM, and prevention of root resorption.
Humans ; Osteoblasts ; Osteoclasts ; Periodontal Ligament ; Periodontium ; Root Resorption ; Tooth Movement Techniques