1.Cloning and expression of the pig skeletal muscle musclin gene.
Weijie WANG ; Hongji LI ; Liqiang HAN ; Yueying WANG ; Jing WANG ; Weihua LI ; Maowang LIN ; Yulei TAI ; Zhiqiang ZHANG ; Meng ZANG ; Yanling WANG ; Guoyu YANG
Chinese Journal of Biotechnology 2008;24(7):1248-1252
We found seven tag sequence with high homology in dbEST by using human musclin gene, and got its cDNA sequence, which consists of 651bp and the open reading frame was 54-452 bp detected by RT-PCR, encoding 132 amino acid residue protein. The new gene has high homology with that of human, mouse and rat, the rate is 87.2%, 77.6% and 77.9%, respectively; the gene fragment was cloned into expression vector pGEX-4T-1, and the recombinant was transformed into E. coli BL21. Induced by IPTG, the fusion protein GST-musclin, a 38.59 kD protein was successfully expressed in E. coli BL21 and identified by Western blotting.
Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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DNA, Complementary
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genetics
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Escherichia coli
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genetics
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metabolism
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Humans
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Mice
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Molecular Sequence Data
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Muscle Proteins
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biosynthesis
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genetics
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Muscle, Skeletal
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metabolism
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Open Reading Frames
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genetics
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Rats
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Recombinant Fusion Proteins
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biosynthesis
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genetics
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isolation & purification
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Swine
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genetics
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Transcription Factors
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genetics