Objective To explore the concordance of nursing grades which are ordered by the nurses and the standards of nursing grade and the inpatients' needs. Methods 120 inpatients were rated using the three methods respectively, which were the nurses' order, the standards of musing grade and Bartbel index, and then compare the concordance of the nursing grades rated by the three. Results Then nurses' order was of well concordance with the standards of nursing grade and Barthel index,and the Kappa value was 0.862,0.611,P<0.01,respectively. Conclusions The nurses' order accords with the standards of nursing grade and the patients' needs, is helpful to develop the clinical work, and should be spreaded and exploited.

0.05).No significant histological and ultrastructural changes were observed in the skin biopsies.Conclusion The long pulsewidth Diode Laser is a safe hair removal technique with good result and without adverse effect on the function of axillary sweat gland excretion.

Objective To valuate the effect of high energy ultrapulse CO2 laser on congenital melanocytic nevi of the orbit. Methods 46 patient with congenital melanocytic nevi of eyelid, 58 le-sions were vaporized with high energy ultrapulse CO2 laser. The area of the largest one was 3.0 cm×6.0 cm, and the smallest one was 0.3 cm×0.3 cm, with average of 1.3 cm×2.0 cm. The depth of the vaporizing reached the layer of lowest skin, even the orbicularis, aponeurosis and conjunctiva of tarsus, till no melanin on the wound in view, tried the best to reserve the normal follicle of eyelash. There was no eschar and bleeding on the wound during the procedure. Followed up lasted for 1 - 56 months, with average 12.9 months. Results 44 of 46 patients were satisfactory to the effect of the laser. The most of scars resulted from the vaporizing with the laser became evenness and smooth (50/58). The edge of eyelid looked normal after the laser. The excellent result was observed in 84.5 % (49/58) lesions, good in 10.3 % (6/58), and improved in 5.2 % (3/58). The overall effect was 94.8 % (55/58). And eyelashes were getting growth. Conclusion The treatment of con-genital melanocytic nevus of eyelid with high energy ultrapulse CO2 is a good method. But to be careful in selecting the lesions that may not he too large.

Objective To evaluate the long-term effects and safety of diode laser in hair removal.Methods A retrospective study was conducted.In this study,350 patients with hypertrichosis were treated by an 800-nm diode laser with a pulse duration of 30 ms for various sessions.Two groups were divided based on the treatment sessions:group A receiving 6(≥4 for axillae)or more treatments,group B less than 6(＜4 for axillae)treatments.Patients were followed up for 8 months to 3 years(mean 22.5 months)by return visit or telephone.Evaluation of efficacy and side effects were performed.Results Follow-up and evaluation were completed in 235 patients,and a total of 375 sites treated.After 2-18 treatments,a total effective rate of 80.53%(302/375)was achieved.Significant higher effective rates were observed in group B compared with group A at all sites 86.84%(33/38)vs 35.00%(7/20)on the lips,68.42%(26/38)vs 30.77%(4/13)on the face and neck,92.00%(46/50)vs 55.56%(5/9)in lower extremities,86.96%(20/23)vs 50.00%(4/5)on the trunk.93.81%(91/97)vs 55.56%(5/9)at the axillae,92.16%(47/51)vs 73.68%(14/19)in the upper extremities(all P＜0.05),Side effects were noted in only 6 cases,including hyperpigmentation,itching,and development of follicular papules,blisters and white hair.Neither hypopigmentation nor scarring was observed.Conclusions Diode laser system is effective and safe for hair removal.The effect varies with lesional sites and treatment sessions.

Total DNA of B. amyloliquefaciens and S. aureus were isolated and cut partially with Hind Ⅲ Separately, The genes for barnase and for staphylococcal nucl ease were obtained by PCR and cloned into pGEM7Z-f(+), The sequencing show that the n ucleotide (nt) sequence of the barnase is 99.3% homologous with the previously determimed sequence and the deduced animo acid (an)sequence found to correspond precisely to the previously determined sequence; the nucleotide (nt) sequence of the staphylococcal nuclease is 99.1% homologous with the previously determined sequence and the deduced amino acid (an) sequence 99.3% homologous with the previously determinded sequence.These have built basis of making use of special expression or activation of nuclease to produce the male sterility plant by plant gene engineering and to explore new antiviral strategy.

Objective. To undertake the pilot experiments of prevention of disability (POD) in 14 different geographical areas to serve as examples for future development of rehabilitation work in China and in Asia. Methods. According to the principles and national criterion, 27 000 people aflected by leprosy were selected and assessed using disability record forms at beginning and followed up regularly for observing changes of different indica-tors. Results. A total of 197 neuritis cases were detected and treated with prednisolone out of 1 407 new or active cas-es. Self-care training of eyes, hands and feet were conducted for 10 500 disabled people affected by leprosy. Compre-hensive therapy was given to 1 804 cases having complicated ulcers of which 1 055 cases have got their ulcers healed.Out of 706 prostheses, 613 were given to patients with satisfactory results. Surgical treatment was given to 269 cases and 251 have shown good progress. Conclusion. Most of patients have got benefit from the project in function or appearance which is very helpful for their going back to the society and agreed by foreign experts during the final evaluation. The experiences from the pro-ject can be implemented in the whole country.

Objective To investigate the application of modified early warning score ( MEWS) to the nursing care of the patients in Emergency Observation Room, and to explore the relationship between outcome and prognosis. Methods According to the convenience sampling method, 331 patients in Emergency Observation Room from a grade A tertiary hospital were assessed by MEWS. The observation factors included the relationship between MEWS at the first day of entering the room and patients′ observation time, MEWS after three days and prognosis. Results Patients′ MEWS at the first day had no correlation with observation time or prognosis ( r=0.052,0.097;P>0.05); patients′ MEWS after three days was related to prognosis(r =0.550, P<0.05). Moreover, the higher MEWS implied the worse conditions and higher mortality. Conclusions MEWS can imply patients′illness outcome and prognosis in order to help nurses to make nursing decision.

Objective To study the clinical effect of electric toothbrush on oral care among patients with orotreacheal intubation. Methods A total of 100 cases of orotracheal intubation were randomly divided into two groups, 50 cases in observation group and 50 cases in control group. The patients of observation group used electric toothbrush with chlorhexidine for oral care, and the patients of control group received the traditional oral care method. Results The oral cavity cleanness (80% ofⅠdegree) in the observation group were significantly better than 24% Ⅰdegree cleanness patients of the control group, while the Ⅱ and Ⅲ degree were 16% and 4% of the observation group were lower than 46% and 30% of the control group (χ2 =26. 606, 10. 519, 11. 977;P<0. 01). The 3rd day of bacteria culture obtained 28% of negative rate lower than that of 56% in the control group (χ2 =8. 046;P<0. 01). The patients of observation group utilized (9. 78 ± 1. 16) min/time compared with (8. 91 ± 0. 96) min/time (t=4. 121,P<0. 01). There was no statistical significance on the difference of adverse event rate between two groups (P>0. 05). Conclusions The clinical effect of oral care using electric toothbrush with chlorhexidine compared with patients with orotracheal intubation, the former one is better than the traditional oral care method.

Objective:To understand the current situation of scientific research ability and training needs of community nurses in China.Methods:The convenient sampling method was used to select 3 633 community nurses from 16 provinces/autonomous regions/municipalities from June to August 2021 to investigate. The contents of the questionnaire included general information, Self-Assessment Scale of Scientific Research Ability for Nursing Staff, scientific research attitude and training needs. A total of 3 633 questionnaires were recovered, of which 3 561 were valid, with an effective recovery rate of 98.0%. Univariate analysis and multiple linear regression analysis were used to analyze the influencing factors of the total score of self-assessment of scientific research ability of community nurses.Results:The total score of scientific research ability of 3 561 community nurses was 39.00 (28.00, 60.00) . The results of multiple linear regression analysis showed that nursing working years, highest education, received research courses/training, working experience in academic organizations/academic journals, participating in academic conferences or above the municipal level in the past 5 years and having plans to improve academic qualifications in the next 5 years were the influencing factors of scientific research ability of community nurses ( P<0.05) , jointly explained 15.4% of the total variance (adjusted R2=0.154, P<0.001) . Community nurses had training needs in data collection and organization, scientific research design, literature retrieval, scientific research topic selection, data statistical analysis and statistical software operation and submission skills. Conclusions:Subjective self-assessment scientific research ability and objective scientific research output of community nurses need to be improved and key scientific research talents need to be cultivated.

Objective To Analyze the clinical outcomes of pediatric liver transplantation (LT) for liver-based metabolic disorders.Methods We conducted a retrospective analysis on 42 pediatric patients with liver-based metabolic disorders from June 2013 to March 2017,and analyzed the pediatric end stage liver disease model (PELD),growth and development,type of transplant,postoperative complications and prognosis of patients.Results There were 42 children with liver-based metabolic disorders (15.56%) out of all the 270 children who underwent LT.The median age was 51.0 months (range,3.4-160.9 months).Of the 42 children,19 received living donor liver transplantation (LDLT),18 cases received deceased donor liver transplantation (DDLT) and 5 cases received domino liver transplantation.1-,2-and 3-year cumulative survival rate of 42 recipients was 97.7%,93.6% and 93.6%,and that of the grafts was 95.3%,91.4% and 91.4%,respectively.As compared with the 194 children with biliary atresia who underwent LT,significant difference was found in PELD and weight Z-score between the two groups.Conclusion Liver transplantation is a valuable option for children with metabolic disorders,and it has gained a better prognosis.