Metabonomics is the branch of science concerned with the quantitative understandings of the metabolite complement of integrated living systems and its dynamic responses to the changes of both endogenous factors (such as physiology and development)and exogenous factors (such as environmental factors and xenobiotics). As a holistic approach, metabonomics detects, quantifies and catalogues the time related metabolic processes of an integrated biological system, ultimately, relates such processes to the trajectories of the pathophysiological events. Ever since its birth in 1999, metabonomics has already been described in more than 800 scientific papers and half dozen patents, amongst which almost 700 papers were experimental articles. Now, metabonomics has been established as an extremely powerful analytical tool and hence found successful applications in many research areas including molecular pathology and physiology, drug efficacy and toxicity, gene modifications and functional genomics, and environmental sciences. This holistic approach has thus become an important part of systems biology and has now evolved to be a unique part in global systems biology. The essence of metabonomics and some of the present applications were reviewed to illustrate the rapid development of this extremely exciting new frontier.

目的观察促进截瘫患者不良情绪转化的相关因素。方法对86例住院截瘫患者进行日常生活能力评定和焦虑、抑郁量表评定,根据评定结果制定康复训练计划,同时开展心理护理;3个月后进行第2次评定。结果约80%的入院患者有不同程度的焦虑和抑郁情绪,经康复训练和心理护理后,患者的焦虑和抑郁情绪均有不同程度减轻。此外,焦虑及抑郁情绪的轻重与病程长短无关(P>0.05),可促进截瘫患者不良情绪转化的相关因素为适宜的环境、日常生活能力提高、良好的心理护理和及时有效的健康教育。结论了解促进截瘫患者不良情绪转化的相关因素有助于有针对性地对患者实施心理护理,有利于患者回归家庭、回归社会。

Objective To investigate the possibility of interventional treatment of placenta accreta. Methods One patient with placenta accreta was treated with uterine arterial infusion of cef and MTX followed by embolization. Results Her placenta discharged through vagina at the 27th day after uterine aterial embolizayiea. No remnant was found in the uterine by Doppler imaging. The patient uneventfully recovered.Conclusions Intervetional therapy with uterine arterial embolization is a new and reliable method for treatment of placenta accheta.

Objective To investigate the effects of glucocorticoid(GC) on the expression of CD80 and CD4+CD25+T cells in peripheral blood lymphocyte of patients with multiple sclerosis (MS). Methods With flow cytometer,the positive rates of CD80 and CD4+CD25+T cells in peripheral blood lymphocyte were detected in 21 acute MS patients pre and post treatment separately,and compared with the nomal control group.The secols of Expanded Disablility Status Scale(EDSS) were compared pre and post treatment in MS patients.Results The positive rate of CD80[(5.031?1.782)%]in the MS patients pre treatment was obviously less than that in control group's [(6.436?2.035)%](P

The study of the relationship between trace elements and drug resistance of tumor has been going on for a long time.It is clear that some trace elements are related to the tumor occurrence and its progress, such as selenium,zinc,copper.So they can be used as an sub index to diagnosis and to evaluate prognosis. Further study in recent years has shown that trace elements are related to the tumor drug risistance. Improveing nourishment of tumor cells may change its drug resistance and influence the curative effect of tumor. The significance of this study is to search for a simple and approach in reducing drug resistance in order to improve the chemotherapeutic effect and the patient′s prognosis.

The three-dimensional organization and anastomoses of lymphatics in the rabbit stomach were demonstrated by scanning electron microscopy of lymphatic corrosion casts. Casting medium is the diluted low viscosity Mercox used for intraparenchymatous injection into the mucosal, submucosal, muscular and serous layers of the stomach. The lymphatic capillaries with blind ends were found in the deep mucosa and the lymphatic capillary networks and lymphatic plexus were observed in all the submucosal, muscular and serous layers. The distinct imprints which correspond to the bicuspid valves of lymphatics and the oval or fusiform impressions of the endothelial nuclei were seen on the casts.

Objective To explore the prevalence and clinical features of hyperuricemia in elderly people and to analyze the influencing factors of hyperuricemia in elderly people. Methods Serum uric acid (SUA) levels in 1500 elderly people were examined by the uricase-peroxidase enzymatic method .Clinical parameters of hyperuricemia group and normal SUA group were comparatively analyzed. Logistic regression was used to analyze the influencing factors of SUA in elderly people. Results In 264 out of 1500 (17.6%) the SUA level were increased. The ratio of male to female in the hyperuricemia group was 256:8. The proportion of the patients with increased SUA levels, mean level of SUA, serum urea nitrogen, serum creatinine and serum triglyceride were all increased with age. As compared with the normal SUA group, the hyperuricemia group were more prone to have adiposis hepatica (P

Objective · To investigate the gender-related metabolomic differences in human saliva. Methods · The saliva metabolomic profiles of 5 male and 5 female healthy volunteers with matched age, body mass index (BMI), living and tooth-brushing condition were acquired using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry. Metabolites were identified using publicly accessible databases and further confirmed individually with standard compounds. Both multivariant and univariate statistics were conducted to find inter-gender differences.Results · Forty-eight metabolites in human saliva were identified including 13 amino acids, 6 choline metabolites, 15 carnitines, 4 sphinganine metabolites,7 lysophosphatidylcholine and 3 organic acids. Levels of phenylalanine, acetylcarnitine, propionylcarnitine, butyrylcarnitine, isobutyrylcarnitine,isovalerylcarnitine and sphinganine were higher in the saliva of females than that of males. Conclusion · Rich metabolic information present in human saliva with significant gender diffference which ought to be taken into consideration in study on the metabonomics of saliva.

Objective To explore the relationship between the damage of neuromyelitis optica (NMO) astrocytes (AS) and the onset of NMO, and investigate the relevance of AS damage with the severity of the patients with functional defect. Methods The levels of aquaprin4-antibody (AQP4 -Ab), glial fibrillary acidic protein (GFAP), apolipoprotein(ApoE),interleukins-6(IL-6), interleukins-10(IL-10), tumor necrosis factor-α(TNF-α) in cerebrospinal fluid (CSF ) and serum of 30 acute NMO patients were tested by means of ELISA. The results were later compared with control group. And analysis of the relevance of the various index of the levels in CSF with the CSF AQP4-Ab level, the acute phase expanded disability status scale(EDSS) score of the NMO group were made. Results (1)The NMO group in CSF and serum AQP4-Ab, GFAP, IL-6 levels were higher than the control group (P < 0.05), and ApoE, IL-10 levels were lower than the control group (P < 0.05). (2)The CSF GFAP, ApoE, IL-6 in NMO group is higher than the serum (P < 0.05), and CSF AQP4-Ab, IL-10 levels were lower than the serum ( P < 0 . 05 ) . ( 3 ) The CSF GFAP , IL-6 levels and the CSF AQP4-Ab level were positively correlated (r=0.749, r=0.526, P<0.05), and the CSF ApoE, IL-10 levels were negatively correlated with CSF AQP4-Ab level(r = -0.571, r = -0.676, P < 0.05). (4)The CSF AQP4-Ab, GFAP, IL-6 levels and the acute phase EDSS score were positively correlated (P < 0.05), the CSF ApoE, IL-10 levels were negatively correlated with the acute phase EDSS score (P < 0.05). Conclusion The AS damage exists in the NMO and the damage severity may correlate with patient function defect. AQP4-Ab, GFAP, IL-6 may play important roles in the onset of NMO and the disease aggravating. The decrease of the ApoE and IL-10 may exacerbate NMO damage.

Purpose:To evaluate the effect of continuous intraperitoneal washing chemotherapy in treating the abdominal cavity tumor of nude mice with human ovarian epithelial cancer.Methods:The abdominal cavity tumor of nude mice models was established with human ovarian serous epithelial cancer cell line SKOV 3. 20 experimental nude mice were divided into 3 groups: the common treatment group(7 mice),the continuous washing intraperitoneal chemotherapy group(7 mice) and the control group(6 mice).All the mice were killed after two courses of treatment and the weight and volume of the cavity tumor were examined. The cell cycle expresstion rate of C erbB2 and Fas of abdominal neoplasms tissues in washing liquid were detected with FCM. Results:In the intraperitoneal washing group,the nude mice's FBW/IBW was 0.886. The volume of the cavity tumor is obviously smaller than the routine group. Significant difference in tumor growth inhibition rate was found between the routine group and the washing group.In the washing liquid, G 1 and S phase ratios were markedly lower ( P 0.05); C erbB2 rates were 66%、59% and 42% in control、common and washing group respectively, which was significant different between all groups ( P