AIM To investigate the protective effect of daidzeid (DZ) on cerebral ischemia and it mechanism in mice METHODS Cerebral ischemia was induced by cutting heads and bilateral carotid arteries were completely or incompletely ligated RESULTS The stroke index score in ischemic mice was reduced by DZ, an the persistent time of gasping of mice by decapitation and survival time of mice by bilateral carotid arteries ligation were prolonged by DZ DZ could reduce the content of brain lactic acid while improve the brain superoxide dismutase activity CONCLUSIONS The results indicate that DZ has the protectiveeffect on cerebral ischemia injury Its mechanism may be related with its effect of decreasing of lactic acid content and reducing of free radicals production

Objective To investigate the outcomes of laparoscopic surgery for endometriosis complicated with infertility and the related factors influencing the post-treatment pregnancy.Methods Between January 2006 and January 2007,104 patients with endometriosis complicated with infertility,including 19 r-AFS stage Ⅰ cases,15 stage Ⅱ cases,36 stage Ⅲ cases,and 34 stage Ⅳ cases,were treated in our hospital.The ectopic endometrial tissues were removed by laparoscopy in all of the patients.And adherent tissues were freed at the mean time.After the procedures,Methylene blue was used to test the patency of the Fallopian tubes.For cases with obstructed Fallopian tubes,salpingoplasty was performed as well.After the operation,the patients received gestrinone therapy for 3 to 6 months and were followed up for pregnancy rate.Results The laparoscopic procedures were completed in all of the 104 cases.The post-operational rate of pregnancy was 38.5%(40/104).In women with stage Ⅰ endometriosis,the pregnancy rate was 42.1%(8/19),while in the stages Ⅱ,Ⅲ,and Ⅳ,the rate was 40.0%(6/15),36.1%(13/36),and 38.2%(13/34),respectively(?2=0.206,P=0.977).The pregnancy rate of the patients with single ovarian chocolate cyst was 54.8%(23/42),and that in the patients with chocolate cyst complicated with ectopic endometrium in other positions or those without chocolate cyst were 22.9%(8/35) and 33.3%(9/27) respectively(?2=8.616,P=0.013).The postoperative pregnancy rate of the patients with chocolate cyst in the right ovary [81.3%(13/16)] was significantly higher than that in the cases with left chocolate cyst [35.7%(5/14)] or chocolate cyst in both sides [41.7%(5/12)](?2=7.412,P=0.025).Conclusions Laparoscopy is effective for endometriosis complicated with infertility with high post-operative pregnancy rate.EMT stages show no effect on the pregnancy rate,which may be associated with the position of ectopic endometrium and the side of chocolate cysts.

Objective To expatiate the ultrasonographical and pathological manifestations of breasts with juvenile macromastia.Methods Four patients with juvenile macromastia were examined by high frequency ultrasonography.The sonographical and pathological images were observed and reported.Results The sonographical characteristics of the breasts with juvenile macromastia were similar: the anatomical structures of the breasts were obscure,which were characterized by cystic formation,reticular structure,patched hypoechoic or hyperechoic regions and distorted gland structure.One patient had fibroadenoma and dermatic erythema.The results of pathological examination showed marked cystic proliferation of glands accompanied with ductal epithelial hyperplasia,irregular ductal distribution,no lobules formation or proliferation of fat tissue,fibrocyte and fibroblast.Conclusions The abnormalities of juvenile macromastia may not only be the huge volume,but also may include abnormal ultrasonographical and pathological manifestations,awaiting more follow-up studies.

Objective To study the value of ultrasonographic diagnosis of galactostasis in lactation.MethodsPatients in lactation and post-lactation were examined by color Doppler ultrasonography.Combined puncture,operation and pathology,the imaging features of different stage were summarized.Results The type of galactostasis included ductal ectasia,galactocele,infection and consolidation.The ultrasonographic examination with imaging features can determine the diagnosis and judge the type and the course of disease.Conclusions Ultrasonography is a first choice in diagnosing galactostasis in lactation.

Objective To observe the effects of Ketangte 2 on experimental diabetes in mice.Methods Adrenalin and streptozocin(STZ) were used to induce hyperglycemia mice model and diabetic mice model respectively.The mice were divided into five groups randomly:model group,glibenclamide control group,high-,middium-,low-dosage Ketangte 2 groups.The administration lasted consecutive 15 days.The blank control group and model group were treated with the same volume of saline.After treatment,the levels of blood sugar were measured in hyperglycemia mice induced by adrenalin,and the fasting blood-glucose,2-hour blood-glucose,insulin levels in diabetic mice induced by STZ were determined.Meanwhile the histopathological changes of pancreas were observed under light microscope.Results For STZ-induced diabetic mice,the hypoglycemic effect of Ketangte 2 was obvious(P

Objective To investigate the clinical application value of ultrasonography in excising breast mass with Mammotome vacuum assisted biopsy device and a stereotactic unit(MMT).Methods Guided by ultrasonography,breast masses were biopsy and excised with MMT.The property of masses were identified by pathology.The effects were followed up with ultrasonography.Results Sixty one masses of breast in 34 cases were excised.Pathology showed that 54 were fibroadenoma and 7 were fibroadenosis.The patients had little discomfort in operation and few complications post operation.There were no relapses during short interviews.Conclusions MMT can biopsy and excise the breast mass guided by ultrasonography.The operation has little wound and few complications.It is a useful minimally invasive method which does not influence the appearance of breast.Ultrasonography can insure operation safety and precision.The effects are evaluated immediately.

Objective To analyze the correlation between the expression of Abl interactor 1 (ABI1) and the clinicopathologic characteristics in colorectal adenocarcinoma tissues.Methods Immunohistochemistry was used to determine ABI1 expression in human colorectal adenocarcinoma tissues and matched adjacent tissues.A statistical analysis was used to determine the potential correlation between ABI1 expression and clinicopathological characteristics and prognosis.Results ABI1 is up-regulated in colorectal adenocarcinoma tissues compared with matched adjacent tissues (P =0.000),ABI1 expression is significantly correlated with infiltration (P =0.043) and differentiation (P =0.040),but not with sex,age,tumor location,clinical stage,lymph node metastasis and distant metastasis (P ＞0.05);It was also shown that ABI1 expression had a significant influence on prognosis (x2 =11.090,P =0.001).Multivariate analyses showed that high ABI1 expression is not an independent poor prognostic factor for overall survival (P =0.119).Conclusion ABI1 overexpression might act as pro-oncogene for patients with colorectal adenocarcinoma.

Objective To investigate the conventional ultrasonic and elastographic characteristics of papillary thyroid micro-carcinomas(PTMC) suffered in normal and Hashimoto's thyroiditis(HT).Methods A total of 227 patients with 249 nodules that were confirmed by surgery or biopsy were included in this study.The cases were divided into normal group and HT group.All patients underwent conventional ultrasound and elastography (static pressure).The sonographic characteristics and strain ratio (SR) value were evaluated.Results In the normal group,the ratio between male and female was 1 ∶ 2.9,while in HT group,it was 1 ∶ 11,which was significant different between the two group (P =0.002).Sonographic characteristics that had no significant difference between the two groups (P ＞0.05) include:boundary,shape,vertical and horizontal ratio,halo,echogenicity,calcification,posterior shadowing,vascularity on color Doppler ultrasonography.The strain ratio value was statistically different between the two groups (P =0.034),with 3.44 ± 1.03 in normal group and 3.13 ± 1.13 in HT group.Conclusions The conventional ultrasonographic features of PTMC were similar in both normal thyroid and HT.However,females suffering HT were more susceptible to PTMC,and the strain ratio value of PTMC concomitant HT decreased.

Objective To compare the curative effect of magnesium isoglycyrrhizinate and compound ammonium glycyrrhetate in patients with liver cirrhosis of decompensated.Methods Eighty-six patients with liver cirrhosis of decompensated were enrolled into the study and randomly divided into two groups: the treatment group(n=43) were given magnesium isoglycyrrhizinate once a day in addition to routine treatment;the control group(n=43) were given compound ammonium glycyrrhetate once a day in addition to routine treatment.The clinical manifestation,including symptoms,signs and hepatic function were observed.The clinical lab data,including blood routine examination,urine routine test and kidney function of all patients from two groups were collected before and after the treatments.The adverse drug reactions were monitored throughout the whole therapy.Results ALT and AST turned to normal in 97.7%(42/43) and 90.7%(39/43) of patients respectively in the treatment group,which were significantly higher than those of 72.1%(31/43) and 74.4%(32/43) respectively in the control group( x2=9.86 and 4.73,respectively,Ps＜0.05). Time to turning to normal in ALT and AST were(21.6±9.1)d and(23.1±10.6)d in the treatment group,which were significantly lower than those of(37.5±17.8)d,and(46.7±19.4)d respectively in the control group(t=5.23 and 7.01,respectively,Ps＜0.01).Conclusion The results suggested that magnesium isoglycyrrhizinate had better effect on alleviating symptoms and decreasing enzyme in treatment of liver cirrhosis of decompensated stage.

BACKGROUND: Apoptosis secondary to ischemia and hypoxia is the main cause of spinal cord dysfunction. Because of the decrease in atmospheric pressure, patients living on the Qinghai-Tibet Plateau are in a hypoxic environment, which is very unfavorable for the recovery of spinal cord injury. Hyperbaric oxygen therapy can improve the postoperative function of patients with incomplete spinal cord injury, and its effect is better on the plateau than at normal altitudes.OBJECTIVE: To observe the effect of hyperbaric oxygen therapy on traumatic spinal cord injury in patients living on the Qinghai–Tibet Plateau.METHODS: This prospective, open-label, randomized controlled clinical trial was performed at the Department of Spine Surgery, Affiliated Hospital of Qinghai University, China. In total, 164 patients with incomplete traumatic spinal cord injury were equally and randomly assigned to a control group and a hyperbaric oxygen therapy group. Patients in the control group were treated with pedicle screw fixation and decompressive laminectomy. In addition to the surgical treatment performed in the control group, patients in the hyperbaric oxygen group underwent hyperbaric oxygen therapy at 0.2 MPa once a day for four treatment courses. Ten treatment sessions constituted one course, and each course was separated by a 5- to 7-day rest interval. The primary outcome was the modified Barthel index to assess activities of daily living. The secondary outcomes were the American Spinal Injury Association (ASIA) impairment scale grade, sensory score, and motor score. The study protocol was approved by the Ethics Committee of the Affiliated Hospital of Qinghai University, China (Approval number: QHC011K). Written informed consent was provided by a relative or legal representative of each patient after they had indicated that they fully understood the treatment plan. RESULTS AND CONCLUSION: The partial results demonstrated that after four treatment courses (55-61 days), the modified Barthel index and ASIA tactile, pain, and motor scores were higher in the hyperbaric oxygen group than in the control group. The ASIA grades were significantly different between the hyperbaric oxygen group and control group. The proportion of patients with ASIA grades D and E was higher in the hyperbaric oxygen group than in the control group. In this trial, we aim to determine the efficacy of hyperbaric oxygen therapy on the treatment of incomplete traumatic spinal cord injury in patients living on the plateau and to provide clinical evidence for treating incomplete traumatic spinal cord injury in these patients.