AIM:To study the effect of Ketangte2 (KTT2)on reducing blood glucose and its mechanism.METHODS:Adrenalin and streptozocin were used to induce hyperglycemia in two mice models,and then treated them with glybenzcyclamide (50 mg/kg),three different dose of KTT2 and normal saline(NS)(0.1 ml/10 g weight)for 15 days.And other healthy mice were set up as control group,During the experiment,the fast blood glucose(FBG),insulin was measured in different time.The pancreas and islets of diabetic mice induced by STZ were studied by pathologic section.RESULTS:In the adrenalin-induced model group,KIT2 could lighten hyperglycemic reaction,which Was significant different from that of model group(P＜0.05 or 0.01),while KTT2 high,middle dose group could decrease blood glucose in streptozocin-induced hypoglycemic mice obviously(P＜0.01).Histological examination showed that pancreatic island number in pancreas in KTT2 groups increased in comparison with the model group.CONCLUSION:KTT2 can obviously reduce the blood glucose in the two kinds of animal models of hyperglycemia (P＜0.05 or 0.01).Its mechanism may be related to the protective effect on islet β cells,thereby increase the insulin secretion.

Objective To evaluate the effect of pidotimod combined with antibacterials on the immunologic functions and clinical curative efficacy for patients undergoing recurrent respiratory tract infections.Methods Patients with recurrent respiratory tract infections (150 cases) were enrolled in Matemal and Child Health Hospital of Guangyuan from August 2013 to January 2016,which divided to two groups randomly,Group A (n =75) accepted traditional antibacterials treatment,and Group B (n =75) adopted pidotimod combined with antibacterials.The clinical symptom and immunologic functions of patients during treatment were tested,and the clinical curative efficacy of patients was evaluated in the follow-up period.Results After treatment,the disappear time of cough,fever,tonsil inflammation and pulmonary wheezing in treatment group was significantly shorter than that of control group (P ＜ 0.05).After treatment,the levels of serum immunoglobulin in two groups were higher than those before treatment (P ＜ 0.05),and the treatment group was significantly higher than control group (P ＜ 0.05).After treatment,the relative activities of CD3+,CD4+,CD8+,and NK cells in two groups increased significantly (P ＜ 0.05),the relative content of CD4+/CD8+ was also increased (P ＜0.05),and the improvement of above indexes in the treatment group was more obvious than those in control group (P ＜ 0.05).After treatment,total effective rate of treatment group was significantly higher than that of control group (P ＜ 0.05),the recurrence of infection in the treatment group is significantly lower than that in the control group (P ＜ 0.05),and the recurrence duration was significantly shorter than control group (P ＜ 0.05).Conclusion The clinical curative of pidotimod combined with antibacterials was remarkable for patients of recurrent respiratory tract infections,which deserved popularization not only improving the immunologic functions,but also decreasing the times of recurrence.

OBJECTIVE:To evaluate the status quo and tendency of the use of nootropics in our hospital.METHODS:The nootropics used in our hospital from 2004 to 2006 were analyzed statistically.RESULTS:The consumption sum of nootropics in our hospital increased year by year,2.03 times in 2005 and 1.46 times in 2006 respectively over their previous year.The consumption sum of Ginkgo biloba extract for nootropics took the lead from 2005 to 2006,over 60% of which were medical insurance drugs.CONCLUSION:Leading the list of nootropics in our hospital is plant amedica,with its consumption sum and DDDs showing the equal importance in drug control,suggesting the great market demand for nootropics.

Objective To preliminarily explore the early process of hepatitis B virus (HBV) infection in HepG2 cells induced by dimethyl sulfoxide(DMSO) ,and provide cytological bases for mechanism study of HBV infection in vitro .Methods HepG cells were divided into the DMSO inducing group and control group ,and were cultured 4 days by DMEM containing 1 .5% DMSO and normal DMEM respectively ;changes of cellular morphology were observed .In addition ,selected ECV304 cells as the negative con‐trol group and treated with DMSO .Cells in the three groups were incubated 2 hours with HBV positive serum after culturing 24 hours ,then trypsin digestive solution ,HepG2 cells and ECV304 cells were collected and polymerase chain reaction (PCR) was used for the determination of HBV DNA .Simultaneously ,the blank control group was set ,and the position of HBsAg on HepG2 cells were detected by using indirect immunofluorescence .Results HepG2 cell volume in the DMSO inducing group was obviously in‐creased .HBV DNA was found in HepG2 cells both in DMSO inducing group and control group ,and DMSO inducing group ex‐pressed much stronger .The results of IIF shown that green fluorescent signals of cell membrane and cytoplasm of HepG 2 cells in the DMSO inducing group were increased obviously ,while the results of HBV DNA and IIF both were negative in the negative con‐trol group .Conclusion DMSO could facilitate adsorption of HBsAg in some extent ,which are benefit for completing the process of early infection .

Objective To explore the value of ultrasound in diagnosis and differential diagnosis of pancreatic mucinous cystade‐noma and serous cystadenoma .Methods Data of tumor features of sixty seven cases of pancreatic cystadenoma ultrasonographic was retrospectively analyzed .The tumor location ,tumor size ,tumor boundary ,tumor shape ,cavity number and calcification were re‐corded .Then all the data was statistically analyzed .Results Pancreatic cystadenoma occured mostly in middle‐aged women ,there was no significant difference between pancreatic mucinous cystadenoma and serous cystadenoma of the pancreas in age ,gender ,le‐sion location ,lesion size ,shape ,boundary ,cystic wall ,cystic wall nodules(P>0 .05) ,while there were significant differences in with and without clinical symptoms ,cavity number ,diameter of the largest cyst and calcification(P<0 .05) .Conclusion Ultrasonic man‐ifestations of pancreatic cystadenoma has certain characteristics ,when there are clinical symptoms ,cavity number is less than 6 , maximum sac diameter is bigger than 2 cm and there is marginal calcification ,it tends to mucinous cystadenoma;when there is no clinical symptoms ,cavity number is equal to or more than 6 ,maximum sac diameter is equal to or smaller than 2 cm and there is cen‐tral calcification ,it tends to serous cystadenoma .

ObjectiveTo study the relationship between subjective well-being and family care degree of medical college students.Methods2695 medical college students were tested by Family APGAR index and Oxford Happiness Test-Revised.The data was analyzed by independent-sample t test,pearson correlations and linear regression.Results ( 1 ) The subjective well-being and family care degree and its factors of college students were above average.The score of subjective well-being was 43.08 ± 11.16,and the score of family care degree was 6.48 ±2.39.(2)The family care degree( t=3.462,P＜0.01) and its adaptation( t=2.718,P＜0.01),partnership( t =4.178,P＜0.01),and growth( t=4.484,P＜0.01 ) of female students were higher than male students.The family care degree ( t =3.939,P ＜ 0.01 )and its adaptation ( t =4.683,P ＜ 0.01 ),partner-ship ( t =3.306,P＜ 0.01 ),and growth ( t =2.796,P ＜ 0.01 ) and subjective well-being( t =3.508,P＜ 0.01 ) of the students who came from cities and towns were higher than the students who came from rural.(3)There was a significant relation between subjective well-being and family care degree and its factors( r=0.197 ～ 0.357,all P＜0.05 ).The partner-ship,resolve,growth and affection can forecast together the 13.2％ variance of subjective well-being.ConclusionEnhancing family care degree is contribute to the subjective well-being and psychological health of medical college students.

Objective To investigate the conventional ultrasonic and elastographic characteristics of papillary thyroid micro-carcinomas(PTMC) suffered in normal and Hashimoto's thyroiditis(HT).Methods A total of 227 patients with 249 nodules that were confirmed by surgery or biopsy were included in this study.The cases were divided into normal group and HT group.All patients underwent conventional ultrasound and elastography (static pressure).The sonographic characteristics and strain ratio (SR) value were evaluated.Results In the normal group,the ratio between male and female was 1 ∶ 2.9,while in HT group,it was 1 ∶ 11,which was significant different between the two group (P =0.002).Sonographic characteristics that had no significant difference between the two groups (P ＞0.05) include:boundary,shape,vertical and horizontal ratio,halo,echogenicity,calcification,posterior shadowing,vascularity on color Doppler ultrasonography.The strain ratio value was statistically different between the two groups (P =0.034),with 3.44 ± 1.03 in normal group and 3.13 ± 1.13 in HT group.Conclusions The conventional ultrasonographic features of PTMC were similar in both normal thyroid and HT.However,females suffering HT were more susceptible to PTMC,and the strain ratio value of PTMC concomitant HT decreased.

Objective To observe the ultrasonic features of cystic renal cell carcinoma. Methods Ultrasonic features of 18 patients of cystic renal cell carcinoma confirmed with operation and pathology were analyzed retrospectively.Results Among 18 patients, 7 were clear-cell carcinoma of cystis degeneration, 5 were cystic renal cell carcinoma (clear cell type), 3 were multilocular renal cell carcinoma, 1 was corpora mammillaria renal cell carcinoma, and one clear-cell carcinoma was on capsule wall of cyst and 1 was nephroblastoma. Ultrasound diagnosed 15 patients and misdiagnosed 3 with a diagnostic rate of 83.33%.Conclusion Cystic renal cell carcinoma has specific ultrasonic characteristics, and having an intimate knowledge of these characteristics may be helpful to diagnosis.

Objective To explore the ultrasonographic appearance of dermatofibrosarcoma protuberans. Methods Clinical data, sonographic features and color Doppler flow imaging characteristics of 12 patients surgically and pathologically confirmed dermatofibrosarcoma protuberans were retrospectively analyzed.Results Single mass was detected in 11 patients (11/12, 91.67%) with the diameter ranged 0.9-8.0 cm. The tumors distributed in the trunk (8/12, 66.67%), proximal limbs (2/12, 16.67%) or face and neck (2/12, 16.67%). Most of the tumors (10/12, 83.33%) developed from skin and subcutaneous fat layer of the trunk and limbs and were hypoechoic and heterogeneous (11/12, 91.67%), some (8/12,66.67%) with distinct boundary and regular shape without liquification, calcification nor regional lymph node metastasis. Affluent color Doppler flow signals of tumor were found in 11 patients (91.67%).Conclusion Ultrasonographic characteristics of dermatofibrosarcoma protuberans include distinct boundary, regular shape, heterogeneous hypoechogenicity and rich color Doppler flow signals without regional lymph node metastasis.

Objective To investigate the relationship of the anti-inflammatory effects of tetramethylprazine with histamine and 5-hydroxytryptaphane(5-HT).Methods The contents of histamine and 5-HT in the inflammatory exudate of rats were determined by fluorescence technique.The effect of tetramethylprazine on histamine and 5-HT were analyzed by in-vitro ileum contraction test in guinea pigs.Results Tetramethylprazine significantly decreased the contents of histamine and 5-HT in the inflammatory exudate,and markedly antagonized the contraction of isolated ileum induced by histamine and 5-HT.Conclusion The anti-inflammatory mechanisms of tetramethylprazine may be related to the inhibition of the synthesis or release of histamine and 5-HT,as well as its direct antagonism.