We report two cases of retroperitoneal fibrosis that emerged during a clinical course of moderate chronic kidney disease. In both cases, we observed an elevation in the serum CRP and IgG4 levels without an increase in the white blood cell count. The patients were treated with prednisolone. Their clinical conditions improved with a decrease in the serum IgG4 to total IgG ratio. The present cases suggest the importance of a differential diagnosis of retroperitoneal fibrosis in the medical care of chronic kidney disease patients, and we propose a useful biomarker for retroperitoneal fibrosis, which we suspect is associated with IgG4-related disease.

The main mechanisms by which acupuncture/moxibustion treatment exerts its effects are "activation of the pain inhibitory system", "influence on tissue perfusion" and "relaxation of hypertonic muscles". Basic research has been conducted on these effects in various fields, and substantial knowledge has been obtained. Furthermore, in clinical studies based on the results of such research, these key expressions are often used to discuss outcomes. As can be seen from this, in most cases, acupuncture/moxibustion treatment is used to cause functional changes rather than organic changes. On the other hand, "regenerative medicine" is a field that requires clear organic changes. The theme of "regenerative medicine and acupuncture/moxibustion" discussed in this symposium is a challenge to enter a new area of medicine that differs from conventional acupuncture/moxibustion treatment. In this presentation, "peripheral nerves", "bones", "tendons" and "muscles" are the focus, and the results of previous research on each are introduced. This paper summarizes the details.

Objective: Histological and dynamic assessment to determine the effect of electroacupuncture on tendon repair in a rat model of Achilles tendon rupture. Methods: Sixty 12-week old male Wistar rats were used to prepare an Achilles tendon rupture model. The rats were randomly divided into an electroacupuncture group (EA group) and a control group. The EA group rats were restrained, and under mild anesthesia two acupuncture needles were inserted with the tip of one touching the ruptured tendon on the outer side, and the tip of the other touching it on the inner side. Using the needle on the inner side as the cathode and the needle on the outer side as the anode, electroacupuncture with intermittent direct current was performed (pulse width: 5 ms, stimulation frequency: 50 Hz, stimulation strength: 20 μA, stimulation time: 20 min). Electroacupuncture was performed every day from the day after model preparation until the days of assessment. Rats in the control group were retrained and mildly anesthetized only. Samples from the region of interest were taken 7 and 10 days after model preparation to assess tendon repair. Tissue imaging was performed, using HE staining to measure total cell count and immunohistochemical staining to measure the number of cells positive for TGF-β1 and b-FGF. Tension tests were also performed 10 days after model preparation to measure the maximum failure load of repaired tendon. Results: HE staining showed a significant difference between the assessment results on different days and between the two groups. A clear increase in the cell count was observed in the EA group (day 7: p<0.05, day 10: p<0.001). Immunostaining showed the strongest expression of both TGF-β1 and b-FGF in the EA group 7 days after model preparation, and other significant difference were observed (TGF-β1: day 7 EA group vs. day 10 EA group: p<0.001, vs. day 7 control group: p<0.0001, vs. day 10 control group: p<0.0001) (b-FGF: day 7 EA group vs. 10 EA group: p<0.001, vs. day 7 control group: p<0.0001, vs. 10 day control group: p<0.0001). Maximum failure load of repaired tendon 10 days after model preparation was significantly higher in the EA group (p<0.01). Discussion: Examination in the early stages after Achilles tendon rupture showed an increased cell count and increased expression of growth factors in the EA group, as well as increased tendon strength. These results indicate the beneficial action direct current electroacupuncture has on cell growth and growth factor expression during tendon repair, and suggest it also increases the dynamic strength of repaired tendon. Direct current electroacupuncture is thought to be possibly useful method for promoting tendon repair.