Excessive drinking causes of social problems and economic loss, and has adverse physical and mental effects. If the gene polymorphism of aldehyde dehydrogenase (ALDH2), an important enzyme in the degradation of alcohol, is known, it is possible to avoid drinking alcohol to a level that exceeds its decomposition ability and prevent adverse physical and social effects. In the future, pharmacy students will contribute to improving the health of local residents as pharmacists; therefore, it is necessary for them to understand alcohol metabolism and related problems. In this study, we investigated the degree of understanding of alcohol degradation pathways, related enzymes, and polymorphisms among second- and third-year pharmacy students in their twenties before and after watching a test-linked video lecture on the subject. Third-year students (61.3 points) scored higher than second-year students (45.7 points) on average due to the learning effect. However, both the second- and third-year students did not show a high level of comprehension before the lecture. After the video lecture related to the test was shown to students, the same test was performed again. Comparing the test scores before and after the video lecture, both the second- and third-year students demonstrated a higher average score (86.1 and 93.3 points, respectively), a significant increase. From these data, it was proved that the test-linked video lecture we created could be used as an effective tool by pharmacy students to understand alcohol metabolism in a short time.