Objective To explore the relationship of leptin、 Glutathione peroxidase (GSH-Px) and other correlative factors with type 2 diabetic fatty liver. Methods From 2003-10 to 2004-01, we examined leptin、GSH-Px、 blood fat in the serum and BMI、FAT% in 23 cases of type 2 diabetic fatty live、30 cases of type 2 diabetic inpatients and 30 healthy people. Compare their level and analyse the correlation. Results (1)The TG、BMI、FAT% of type 2 diabetic fatty liver patients were significantly higher than that of the others groups. (2)Leptin and GSH-Px of each group had significant changes. Leptin was correlative with FAT% and age. GSH-Px was correlative with age and HDL. Conclusion (1)TG、 BMI and FAT% are important risky factors. (2)There are leptin resistance in type 2 diabetic fatty liver and type 2 diabetes patients and it is influenced by obesity and age. (3)The antioxidant enzyme in the serum of type 2 diabetes patients is reduced and is related with age and HDL.

Objective To evaluate the outcome of a new modality of operation—tensionfree vaginal tape (TVT) in treatment of female stress urinary incontinence. Methods Thirteen female patients suffering from stress urinary incontinence were treating with TVT operation.All the patients were confirmed to have stable bladder without outlet obstruction via clinical routine and urodynamic examination before operation.TVT tape of Prolene was implanted around mid-urethra via a minimal vaginal incision. Results The mean operation time was (38?7)min and the mean intraoperative bleeding was (45?10)ml.The mean catheterization time was 2 days and mean hospital stay was 4 days.No major bleeding was observed in operation.All patients were cured and the clinical symptoms disappeared after operation except for one patient with post-operative urine retention.This patient was catheterized for 2 weeks and the tape was cut under local anesthesia;then the urine retention disappeared and no incontinence relapsed. Conclusions TVT is a simple,reliable mini-invasive procedure with low incidence of complications.It can be used routinely for treating female stress urinary incontinence.

Objective To study the renal protective effects of ulinastatin in rats with multiple organ dysfunction syndrome(MODS). Methods Thirty-six Wistar rats were divided into 3 groups:control group ,model group and treatment group. The MODS models were produced by injecting lipopolysaccharide(LPS) through the sublingual vein into the rats. Ulinastatin was injected into the sublingual vein of the rats in treatment group, LPS was injected into rats in the same way after 10 minutes. On the second hour and the sixth hour after treatment, serum and renal tissue samples were collected from the rats to determine the level of TNF-?,IL-6, IL-1?, iNOS by RIA.The pathologic changes of renal tissue was examined. Results The levels of TNF-?,IL-6, IL-1?, iNOS of serum and renal tissue in treatment group were obviously lower than in model group (P

Objective To investigate the association between gene polymorphism of plasminogen activator inhibitor (PAI-1)and ischemic cerebrovascular disease in aged people of Henan Han nationality.Methods A case control method was used,including 408 patients with ischemic cerebrovascular disease and 418 age and gender matched healthy controls.Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method was used to determine the distribution of allele and genotype frequencies of PAI-1 844G/A.Results In patient group,the gene frequencies of AA,AG,GG were 18.1％,41.4％,40.4％,and the A and G allele frequencies were 38.8％ and 61.2％,respectively.In the control group,the gene frequencies of AA,AG,GG were 15.8％,47.4％,36.8％,and the A and G allele frequencies were 39.5％ and 60.5％.There were no significant differences in the frequencies of genotypes and alleles between the two groups (P＞0.05).The different genotypes of 844G/A of PAI-1 gene were not associated with hypertension,hyperglycemia,plasma homocysteic acid levels (P ＞ 0.05).Conclusions PAI-1 gene-844G/A polymorphism may not be independent risk factor of ischemic cerebrovascular disease in aged people of Henan Han nationality.

Objective To explore the difference of autobiographical memory (AM) between depressive patients with and without alexithymia,and correlation between AM and alexithymia in depressive patients with alexithymia.Methods 29 depressive patients with alexithymia,34 depressive patients without alexithymia were enrolled.All patients were measured by autobiographical memory test (AMT),Toronto alexithymia scale-20 (TAS20),Hamilton depression scale-24 (HAMD-24).Results ①The negative overgeneral AM score was higher in depressive patients with alexithymia than those without alexithymia ((3.34± 1.80),(2.51 ± 1.12),respectively t=2.19,P＜0.05).②The total overgeneral AM score was higher in depressive patients with alexithymia than those without alexithymia ((6.14±2.44),(4.86±2.06),respectively t=2.21,P＜0.05).③)The correlation coefficient (r value) between the total score of overgeneral AM and total score of TAS,TAS-Ⅲ scores of TAS in depressive patients with alexithymia were significantly positively correlated (r=0.240～0.611,P＜0.05).However,the correlation coefficient between the total score of specific AM and total score of TAS,TAS-Ⅲ scores of TAS in depressive patients with alexithymia were significantly negatively correlated(r=-0.502～-0.205,P＜0.05).Conclusion Compared with depressive patients without alexithymia,the overgeneral AM in depressive patients with alexithymia is more severe,and the alexithymia can increase the severity of overgeneral AM.

OBJECTIVE: To investigate the effects of Wenyang Huoxue Recipe (WRHXR), a compound traditional Chinese herbal medicine for warming yang and promoting blood flow, on the expression of angiopoietin mRNA in rats with chronic aristolochic acid nephropathy induced by Caulis Aristolochia Manshuriensis (CAM) decoction, and to explore the protection mechanism of WYHXR against kidney damage. METHODS: Twenty-eight male SD rats were randomly divided into normal control group, CAM group and WYHXR-treated group. Rats in the normal control group (n=8) and CAM group (n=10) were intragastrically administered with normal saline 10 ml/(kg.d) or CAM decoction 10 ml/(kg.d) respectively. Rats in the WYHXR-treated group (n=10) were intragastrically administered with WYHXR 30 g/(kg.d) and CAM decoction 10 ml/(kg.d). The expressions of Ang-l and Ang-2 mRNAs were detected by real-time reverse transcription polymerase chain reaction (RT-PCR) after 20-week treatment. RESULTS: Compared with the normal control group, the expression of Ang-l mRNA was significantly decreased, and the expression of Ang-2 mRNA was significantly increased in the CAM group (P<0.01). Compared with the CAM group, the expression of Ang-l mRNA was increased in the WYHXR-treated group (P<0.01). The expression of Ang-2 mRNA had no significant difference between the CAM group and the WYHXR-treated group (P>0.05). Renal pathology showed that renal damage in WYHXR-treated group was significantly reduced as compared with the CAM group. CONCLUSION: WYHXR can up-regulate the expression of Ang-l mRNA, which may be its action mechanism in protecting the kidneys.

Objective To analyze the efficacy of a second dose of measles-mumps-rubella (MMR) vaccine in children aged 6 to 7 years in Xicheng District, Beijing, China in 2016.Methods Serum samples were collected in duplicate from 41 children aged 6 to 7 years before and one month after immunization with a second dose of MMR vaccine.ELISA was performed to detect IgG antibodies against measles, rubella and mumps in serum samples.Results The positive rates of antibodies against measles, rubella and mumps in the 41 children before immunization with a second dose of MMR vaccine were 95.12%, 90.24% and 92.68% and the antibody titers were (825.88±499.91), (53.83±32.48) and (697.83±535.82) IU/ml, respectively.The positive rates of the three antibodies were 100%, 97.56% and 100% after revaccination with MMR vaccine and the antibody titers were (1 102.98±356.77), (95.08±26.88) and (3 383.85±1 903.06) IU/ml.Titers of antibodies against measles, rubella and mumps increased significantly following revaccination (P<0.05), while no significant difference in the positive rates of the antibodies was observed before and after revaccination (P>0.05).Conclusion The second dose immunization of MMR vaccine achieves a high successful rate in children in Xicheng District of Beijing.It would be an effective immunization strategy to protect children from measles, rubella and mumps.

Aim To investigate the effect of α1-anti-trypsin Z variant (ATZ)overexpression on cell autoph-agy.Methods HEK 293T cells were transfected with pcDNA3.1 zeo+/ATM or pcDNA3.1 zeo+/ATZ,e-qual amount of empty vector was used as control.Cells were treated with NH4Cl for 4 hours and processed for detecting ATZ,LC3 and p62 by immunoblot.Mean-while ,expression and intracellular localization of ATZ, LC3 in 293 T cells were observed with double labeled immunofluorescence.The mRNA levels of autophagy-related genes were measured by real-time PCR.Immu-nohistochemistry was used to observe the morphology of ATZ-positive cells.Results Compared with the control,higher LC3Ⅱ levels and LC3 puncta were observed in ATZ transfected cells.Meanwhile,the levelsof p62 were decreased in ATZ transfected cells,andreversed by NH4 Cl (25 mmol·L -1 )treatment.Overexpression of ATZ increased the mRNA levels of Atg5and Atg12,but had no obvious influence on Beclin1.ATZoverexpressing cells presented abnormal morphologies.The nuclei became reduced,condensed,and even disappeared in ATZpositive cells.Conclusion ATZ overexpression increases autophagy activity whichmay be related to increasing Atg5 and Atg12 levels.

Objective To recheck the reliability of methotrexate ( MTX) serum concentration at 48 h ( C48 h ) in predicting the pharmacokinetic characteristics and toxic reactions at terminal elimination phase after high dose MTX infusion and to provide a reference for determination of rational rescue regimen in clinic practice. Methods In total,114 cases of children with acute lymphoblastic leukemia (ALL) received 176 courses of high dose MTX chemotherapy treatment. The regimen was continuous infusion of MTX[3 -5 g·( m2 ) -1 ] in 24 h. Plasma samples were treated with solid phase extraction and serum concentrations of MTX were determined by HPLC at 24,48 and 72 h (C24 h ,C48 h and C72 h ) after starting MTX infusion. All data were divided into C48 h≥1 μmol·L-1 group and C48 h<1 μmol·L-1 group. The pharmacokinetic parameters of the two groups at elimination phase were estimated by residual method and the toxic reactions after MTX infusion of two groups were compared by Ridit analysis. Results The C72 h and AUC48-∞ were significantly higher in C48 h ≥1 μmol · L-1 group than in C48 h <1 μmol·L-1 group (P<0. 01). The MTX toxicities to the blood,digestive and hepatic systems were significantly higher in C48 h≥1 μmol·L-1 group than in C48 h < 1 μmol · L-1 group ( P < 0. 05). Conclusion C48 h can predict the pharmacokinetic characteristics and toxic reactions at ther terminal elimination phase. Therefore,C48 h≥1 μmol·L-1 can be used as a marker of MTX elimination delay event to guide later rescue regimen.

Objective To investigate the effects ofBushen Gujing Recipe in treating early diabetic nephropathy (DN).Methods Sixty patients of early DN with Yin-Yang deficiency syndrome were randomly divided into treatment group and control group, 30 cases in each group. The control group was treated with conventional western medicine treatment, and the treatment group was treated withBushen Gujing Recipe combined with the conventional therapy. The treatment course was three months. The clinical efficacy, integral of TCM syndrome, and urinary albumin excretion rate (UAER) of the two groups were compared.Results After the treatment, the integral of TCM symdrome of the treatment group was lower than control group, showed a statistically significant difference (P<0.05). The total effective rates of clinical efficacy were 81.48% (22/27) in treatment group, and 64.00% (16/25) in control group. The therapeutic effect of treatment group was better than control group (P<0.05). UAER showed a statistically significant difference between two groups (P<0.05).Conclusion Based on the western medicine treatment,Bushen Gujing Recipe can improve the symptoms of patients, alleviate the injury of kidney, and has good efficacy on DN.