Objective To explore the psychological status of nursing students of Tibetan and Han nationality.Method The questionnaire of Symptom Checklist 90(SCL-90)was distributed to 120 nursing students of Tibetan and Han nationality by random sampling.Results SCL-90 total scores of Tibetan nursing students and Han nursing students were(136.96±30.76)and (129.78±28.99),respectively.There was a statistically significant difference(P<0.001).The scores of Tibetan nursing students was higher than that of Han nursing students on factors of somatization,force symptoms,sensitivity to social relations,depression, hostility,paranoid and psychosis,but the scores of Han nursing students were higher than Tibetan nursing students on factors of anxiety and terror,with significant significance(P<0.01).The results of SCL-90 showed that the scores of somatization,force symptoms,sensitivity to social relations depression,paranoid and hostility in the Tibetan nursing students of 1st grade were significantly higher than those in the Hannrusing students of the 1st grade score(P<0.05).The score of force symptoms,depression and psychosis in the Tibetan nursing students of 2nd grade were significantly higher than those in the Han nursing students of 2nd grade (P<0.05).The scores on force symptoms,sensitivity to social relations,depression and anxiety in Tibetan nursing students of the 3rd grade were higher than those in the Han nursing students of the 3rd grade(P<0.05).Conclusion Tthe psychological status of Tibetan nursing students is outstanding in contrast to Han nursing studetns,and Tibetan nursing students were given directed intervention of mental health problem according to their risk factors.

Ohjective To investigate the associations between the Arg389Gly polymorphism of the β_1-adrenergiecreceptor gene (ADRB1) and vasovagal syncope (VVS) in Chinese children. Methods Genotype of ADRB1 was determined by polymerase chain reaction-restriction fragment length pelymorphism analysis. Case-control studies and quantitative trait analysis were carried out by comparing between carriers (one or two copies of the Gly389 allele) and non-carriers (Arg389 genotype) of the ADRBI in 54 patients with unexplained syncope and in 54 healthy control subjects. Patients were subdivided into two groups according to head up tilt test (HUTT) : positive HUTT, known as VVS group and negative HUTT group. Distribution of Arg389Gly genetype in VVS group and the relationship to three clinical patterns were also analyzed. Results An allele frequency of Arg389 was 73.15% and Gly389 was 26.85% in healthy subjects. Higher Gly389 allele frequency was found in VVS group (n = 30) than that in negative HUTT group (33.33% vs. 14.58%, P < 0.05). In VVS group, the frequencies of the Gly389 allele in cardioinhibitory pattern (n = 6), mixed pattern (n = 9) and vasodepressor pattern (n = 15) was 66.67%, 33.3% and 23.33%, respectively, which had significant differences between the cardioinhibitory pattern from any of the other two patterns (both P < 0.05). Conclusions An association of positive HUTT with a single nucleotide pelymorphism of Gly to Arg switch at position 389 of the ADRB1 was found. This polymorphism may contribute to susceptibility to VVS.

Objective To study serum albumin levels in children with severe sepsis and to correlate serum albumin levels with patient outcome and to identify the causes inducing hypoalbuminemia and its effective countermeasures.Methods Seventy-five children admitted to PICU of Provincial Hospital Affiliated to Shandong University for severe sepsis were included in the study from Aug 2010 to Sep 2011.According to their serum albumin levels within 24 hours and on the third and the seventh day of admission to PICU,75 children were divided into hypoalbuminemia group and normal serum albumin group.Then hypoalbuminemia group was divided into instant hypoalbuminemia group and continuous hypoalbuminemia group according to the duration of hypoalbuminemia.The correlation between the occurring and duration of hypoalbuminemia with patients' prognosis,the etiopathogenisis of hypoalbuminemia and its effective countermeasures were analyzed.Results (1) Sixty-three cases (84.0％) proceeded hypoalbuminemia and their mortality was 33.3％ (21/63),while 12 cases (16.0％) showed normal serum albumin level and their mortality was 0.(2) In 63 patients with hypoalbuminemia,26 cases showed continuous hypoalbuminemia and their mortality was 46.0％,while 37 cases proceeded instant hypoalbuminemia and their mortality was 15.4％.There was significant difference (x2 =5.116,P ＜ 0.05) between their mortality.(3) In the 63 cases with hypoalbuminemia,32 cases presented with hepatic injury and their mortality was 37.5％,13 cases proceeded capillary leakage and their mortality was 23.1％,and 18 cases displayed hepatic injury complicated with capillary leakage and their mortality was 33.3％.There was significant difference (x2 =7.812,P ＜ 0.05) between the mortality with different causes.Conclusion Hypoalbuminemia influenced the prognosis of children with severe sepsis,the longer duration correlated with the worse prognosis.Hepatic injury and capillary leakage were two main causes inducing hypoalbuminemia.Active treatment of hypoproteinemia aimed directly at different causes could improve their prognosis significantly.

Objective To study the relationship between APOBEC3G mRNA level in peripheral blood mononuclear cells (PBMC) and serum hepatitis C viral RNA level in patients with chronic hepatitis C infection. Methods TaqMan real-time fluorescence relative quantitative polymerase chain reaction (RT-PCR) was used to quantify APOBEC3G mRNA levels in PBMC from 49 patients with chronic hepatitis C (CHC) and 31 healthy subjects. The relationship between APOBEC3G mRNA level and hepatitis C virus (HCV) viral load was analyzed. SPSS11. 0 statistics software was used for t test and regression analysis. Results APOBEC3G mRNA level in CHC patients [(1.5×10-5±1.9×10-5 ) copy/mL] was significantly lower than that [( 5. 2 × 10-5 ± 5. 5 × 10-5 ) copy/mL] in the healthy control subjects (t=-3.005, P＜0.01). While APOBEC3G mRNA level was not related with HCV viral loads (r=-0.082, P＞0.05). Conclusion HCV has an inhibitive effect on APOBEC3G expression, whereas APOBEC3G doesn't affect HCV replication directly in vivo.

Objective To explore the factors related to vasovagal syncope (VVS) in children. Methods The clinical data of 125 children with conifrmed VVS were collected. According to the frequency of syncope during the ifve years from ifrst episode to the time of head-up tilt test, the children with 2 or 3 episodes of syncope were assigned into the low episode group, and the children with 4 or more episodes of syncope were assigned into the high episode group. The two groups were analyzed and compared. Results Among the 125 children, 84 children (67.2%) were in the low episode group and 41 children (32.8%) were in the high episode group. The single factor analysis showed that the age at head-up tilt test, onset of syncopal, causes of syncope, history of carsickness, and positive family history were associated with high attack frequency. The results of non-conditional logistic regression analysis showed that causes of syncope (OR?=?3.723, 95%CI:1.163-11.918, P?=?0.027), history of carsickness (OR?=?5.929, 95%CI:2.066-17.015, P?=?0.001), and positive family history (OR?=?6.794, 95%CI:2.006-23.013, P?=?0.002) were the independent risk factors of high attack frequency. Conclusions The causes of syncope (excluding persistent standing), history of carsickness, and positive family history have important clinical signiifcance in predicting high attack frequency of VVS in children.

OBJECTIVETo survey the quality of life in children and adolescents with type 1 diabetes.

METHODSNinety-eight children and adolescents with type 1 diabetes who participated in Diabetes Summer Camp held in Chongqing, Wuhan and Cheng during 2012 April and December were recruited in the study. The American juvenile diabetes patients quality of life scale Diabetes Quality of Life for Youths was used to assess the quality of life and SPSS19.0 was used for statistical analysis.

RESULTSThe scale had satisfactory reliability and validity with a Cronbach's Alpha score of 0.942 and a validity score of 0.679. All three dimension of scales: scales of impact, scales of worries and scales of satisfaction were significantly correlated with self-health assessment (P<0.01). The scores of impact and worries accounted for >50% of total scores as the same for the self health assessment scores. The score of disease course, diet and blood glucose control were positive correlated with each other. Age and HbA1c were positively correlated with the scale of impact, while gender has negative correlation with satisfaction scale (P<0.05). The diabetes diet had significant effects on the quality of life.

CONCLUSIONThe quality of life in children and adolescents with type 1 diabetes is decreased, especially for those with longer disease course and female adolescents. The form of Diabetes Quality of Life for Youth used in the study has good reliability and validity, which can reflect the quality of life of Chinese diabetic children and adolescents.

Adolescent ; Child ; Diabetes Mellitus, Type 1 ; Female ; Humans ; Male ; Quality of Life ; Sickness Impact Profile ; Surveys and Questionnaires ; Young Adult

Objective To observe the effects of comprehensive treatment of aerobic exercise and medicine on the levels of serum immunoglobulins and cytokines in rats with chronic abacterial prostatitis (CAP).Methods Forty healthy adult male SD rats were randomly divided into the normal control group,CAP model control group,exercise treatment group,medicine treatment group and exercise and medicine comprehensive treatment group,8 cases in each group.The CAP rat model was prepared by the injection of Xiaozhiling,and after 7 d of rat model construction,the exercise treatment group and exercise and medicine comprehensive treat ment group conducted once swimming exercise at fixed time every day,6 d per week for 4 continuous weeks;the medicine treatment group and exercise and medicine comprehensive treatment group were given Qianlieshutong Capsule aqueous solution for gavage treatment every day;the normal control group,model control group and exercise treatment group performed gastric gavage with sterile normal saline.On 14,28 d of the treatment process the rats in each group were sacrificed.The indicators such as serum immunoglobulins and cytokines in rats were detected by using ELISA.Results On 14 d,compared with the normal control group,the levels of serum IgG,IgA,IgM,TNF-α,IL-1β and IL-6 in each experimental group were increased significantly (P＜ 0.05),while which had no statistically significant difference between the exercise treatment group and model control group and between the comprehensive treatment group and medicine treatment group(P＞0.05);on 28 d,the levels of IgG,IgM,TNF-α,IL-1β and IL-6 in the exercise treatment group were significantly lower than those in the model control group(P＜0.05),the levels of IgG,IgA,TNF-α,IL-1β and IL-6 in the comprehensive treatment group were significantly lower than those in the medicine treatment group (P＜0.05),moreover were close to those in the normal control group(P＞0.05).Conclusion Aerobic exercise can reduce the levels of serum immunoglobulins and cytokines in CAP rats,and the effect is more obviousin CAP rats treated by comprehensive therapy combined with medicine.

Objective To observe the effect of supplemental Revolvin D1 (RvD1) on Toll-like receptor 4 (TLR4) in skeletal muscle of type 2 diabetic mice.Methods 35 male C57BL/6 mice were randomly divided into control group (NC group) and high glucose and high fat diet group.After 8 weeks,mice in high glucose and high fat diet group were given intraperitoneal injection of streptozotocin (STZ) 100 mg/ kg.Then they were randomly divided into two groups:Type 2 diabetes group (T2DM group) and type 2 diabetes + RvD1 intervention group (T2DM + RvD1 group).Mice in T2DM group mice were injected with phosphate buffer saline 0.2 ml and T2DM + RvD1 group mice were injected with Revolvin D1 100 ng/day respectively.The levels of fasting blood glucose,serum insulin and inflammatory factors were detected.The mRNA expression level of TLR4 was detected by real-time quantitative polymerase chain reaction (RT-qPCR) method,and the expression of TLR4 protein was detected by Western blot.Results The levels of insulin resistance index,interleukin(IL)-6 and tumor necrosis factor-α (TNF-α) in T2DM group and T2DM + RvD1 group increased (P ＜ 0.05).Compared with the T2DM group,the levels of insulin resistance index,IL-6 and TNF-α in T2DM + RvD1 group decreased (P ＜0.05).The expression of TLR4 protein in T2DM group and T2DM + RvD1 group was higher than that in NC group (P ＜ 0.05).The expression of TLR4 protein in T2DM + RvD1 group,was lower than that in T2DM group (P ＜0.05).The mRNA level of TLR4 in mice was consistent with the above results by RT-qPCR.Conclusions Moderate supplementation of RvD1 can not only decrease the level of inflammatory factors in type 2 diabetic mice,but also reduce the expression of TLR4 and insulin resistance in skeletal muscle of type 2 diabetic mice.

Vasovagal syncope (VVS) is the most common cause of unexplained syncope in children. The etiology of VVS is complex, and its diagnosis is mainly based on clinical manifestations and head-up tilt test (HUT) . It has been pointed out that neuroendocrine system plays an important role in the pathogenesis of syncope. HUT-induced syncope is accompanied by abnormal neurohumoral activation. The changes of plasma concentration of endothelin-1 and nitric oxide secreted by endothelial system before and after VVS syncope in children suggest that endothelial system is involved in the occurrence of VVS in children. This article reviews the research on the involvement of vascular endothelial system in the pathogenesis of VVS in children.

Systemic immunoglobulin light chain amyloidosis is a protein-misfolding disease caused by the highly organized amyloid fibrillar aggregates that lead to irreversible organ dysfunction. This review summarizes systemic therapies for this disease according to the disease risk stratification. Patients with low-risk systemic immunoglobulin light chain amyloidosis are eligible for chemotherapy combined with autologous hematopoietic stem cell transplantation. Patients with high-risk systemic immunoglobulin light chain amyloidosis can be treated with protease inhibitors (such as bortezomib, carfilzomib and ixazomib) , immunomodulatory agents (such as lenalidomide, pomalidomide) and new immunological agents (such as daratumumab and NEOD001) .