Objective To investigate the effect of indomethacin on the protein expression profile of colorectal cancer cell line HCT116 xenografts in nude mice, and to screen the indomethacin anticancer-related proteins. Methods The nude mice with colorectal cancer cell line HCT116 xenograft were treated with or without indomethacin, and then the total proteins extracted from xenografts were separated and visualized by immobilized pH gradient two-dimensional gel (2-DE) electrophoresis. The differential expression proteins between the experimental and control groups were analyzed using PDQuest image analysis software, and identified by peptide mass fingerprinting based on matrix-assisted laser desorption ionization time of flight mass spectrometry and database searching. Results The 2-DE patterns with high resolution and reproducibility were obtained, displayed about 1100 protein spots in each gel, and the matching rate in the two groups was 96. 0% and 93. 6% , respectively. Thirty-one differentially expressed protein spots were found between the two groups, of which 25 spots decreased and 6 increased in the expression levels in the experimental group than in the control group. Eighteen peptide mass fingerprinting maps were obtained, and twelve proteins including galectin-1, annexin and trancription factor, were primarily identified after database searching, most of which were involved in tumor cell proliferation, invasion, apoptosis and immunity. Conclusions Indomethacin exerts its inhibiting effects on colorectal cancer through regulating multiple proteins expression directly or indirectly. The data will be helpful to elucidate the mechanism inhibiting cancer by indomethacin, and to find new targets of chemotherapeutic drugs.

Objective To investigate the effect of microRNA-101 (miRNA-101) on atrial fibrosis in human chronic atrial fibrillation (AF). Methods Right atrial appendages were obtained from 59 patients (30 with AF) undergoing cardiac surgery, including 47 patients with valve heart disease and 12 patients with congenital heart disease. The expression of miRNA-101 was determined by quantitative real-time PCR in the right atrial appendages of patients with and without AF. The cell-specific localization of miRNA-101 was detected by in situ hybridization assay. The mRNA and protein expression levels of transforming growth factor β typeⅠreceptor (TGFβRⅠ) and collagen type I (COL1) were determined by quantitative real-time PCR and Western-blot assay, respectively. Collagen in the right atrial appendages was observed by Masson staining assay. Results The expression of miRNA-101 was found to be significantly down-regulated in AF patients compared with patients with sinus rhythm (SR) (P < 0.05). The result of miRNA-ISH showed that miRNA-101, which was highly distributed within the connective tissues of heart, was down-regulated at about 24.9% in patients with AF compared with patients with SR. No significant differences at the mRNA expression level of TGFβRI was found between patients with AF and patients with SR (P > 0.05). But the protein expression of TGFβRI in patients with AF was significantly higher than that of patients with SR (P < 0.05). The mRNA and protein expressionsl of COL1 were significantly higher in patients with AF than thoset of patients with SR (P < 0.05). The collagen was significantly increased in patients with AF than that of patients with SR (P < 0.05). Conclusions Downregulation of miRNA-101 may contribute to atrial fibrosis in human atrial fibrillation by targeting TGFβRⅠ.

Penetration enhancers are substances to improve the rate or amount of transdermal permeation which is an important factor in transdermal drug delivery systems (TDDS). Recent researches have found that some of the new penetration enhancers have a higher penetration-effect, little irritation, fewer adverse reactions, and stable properties. In this article, domestic and foreign research reports on penetration enhancers have been collected and summarized. The research progress of penetration enhancers were reviewed, with a purpose to provide a reference for reasonable selection of penetration enhancers.

Objective To assess the diagnostic value of combining aortic dissection detection risk score (ADDRS)with D-dimer in patients with suspected acute aortic dissection(AAD). Methods We retrospectively identified 525 patients with chest pain and with calculated ADDRS and available D-dimer on admission between January 2015 and December 2016. ADDRS ≤1 was defined as ′low probability′ and that >1 as ′high probability′. Results AAD were diagnosed in 106(20.2%)patients. In patients with ADDRS of 0,1 case of AAD was identi-fied and in those with ADDRS of 1,40 were identified while in 76 patients with ADDRS of 2 and 3,71 cases of AAD were identified. D-dimer with threshold of 0.5 mg/L had a negative predictive value of 100%and a specificity of 76.6% in patients with ADDRS of 0. In patients with ADDRS of 1,D-dimer had a negative predictive value of 98.6%. D-dimer showed a negative predictive value of 99.6% and a positive predictive value of 22.3% in patients with low probability. Conclusions High ADDRS benefits confirming AAD. Low ADDRS combined with negative D-dimer is safe and efficient for ruling out AAD ,while low ADDRS with positive D-dimer needs further aortic imaging.

Objective To study MR findings in 20 children aged from 10 months to 14 years with mitochondrial encephalomyopathy.Methods MRI evaluations were done in 20 children with mitochondrial encephalomyopathy proved by muscle biopsy and biochemical laboratory examinations.Results Brain parenchymal lesions in all cases were low signal intensity on T 1-weighted and high signal intensity on T 2-weighted images.Brain atrophy was showed in different degrees in 8 children.18 patients had involvement of gray matter,10 had only the deep gray matter involved and 4 cases had both the deep gray matter and the cerebral cortex involved simultaneously.4 patients showed deep gray matter abnormalities and cerebral infarction with involvement of cortex and subcortical white matter.2 patients had exclusively involvement of white matter,which were nonspecific white matter changes of the trigonal area.Conclusion MRI findings in mitochondrial encephalomyopathy are varied when gray matter especially deep gray matter involved,brain atrophy,untypical infarction and involvement of peripheral white matter are showed on MR,and associated with a variety of neuromuscular symptoms in children mitochondrial encephalomyopathy should be consider.

Objective We retrospectively studied the MRI and MRA appearances of 10 patients,(6 children,4 adults) with Moyamoya disease.Methods MRI and MRA images were analyzed.For the MRA images,it was concerned that whether there were occlusive changes of the internal carotid artery and middle cerebral artery and whether there were collateral vessels.For the MRI images,the locations of the infarct lesions were studied.Results For the MRA images,the internal carotid arteries and the middle cerebral arteries were observed stenosis,and collateral vessels were formed in cerebral hemispheres.There were no remarkable differences between the children and adult groups with regard to the occlusive changes or to the flow void sign on MRA.However,Moyamoya disease in children exhibited a significant increase in the cortical and subcortical infarction(75%,9/12).In contrast,there were much more deep white matter infarctions in the centrum semiovale and basal ganglia in the adult group(87.5%,7/8)than in the children group(33.3%,4/12).Conclusion MRI and MRA are the method of choice in diagnosis of Moyamoya disease.The differences in the site of infarctions between the childhood and the adult Moyamoya disease observed on MRI may explain the differences in the clinical presentation.

Objective To assess the diagnostic value of dynamic contrast enhanced MR imaging in predicting the efficacy of distinguishing benign from malignant soft tissue tumor Methods Dynamic contrast enhanced MR imaging was performed in 30 patients with pathologically confirmed soft tissue tumors The MR signal intensity of the ROI was plotted The slope value of the time intensity curve was analyzed Results There was significant difference between the slope value of benign and malignant lesions Based on the slope value of half minute, differentiation of benign from malignant lesions was possible with 90 9% of the sensitivity, 89 4% of the specificity, 83 3% of the positive predictive value and 94 4% of the negative predictive value Conclusion Dynamic contrast enhance MR imaging can correctly predict benignity or malignance of soft tissue tumors

OBJECTIVE: To investigate the clinical feature, diagnostic and therapeutic methods of inflammatory myofibroblastic tumor(IMT). METHOD: One case of IMT was reported and the relevant literatures were reviewed. RESULT: The computed tomography scan showed irregular soft tissue density shade and aggressive bone destruction with unclear boundary. Pathological findings showed variable numbers of inflammatory cells and myofibroblastic spindle cells. Tumor cells were immunoreactive for vimentin and smooth muscle actin, but negative for desmin et al. CONCLUSION IMT of the maxillary sinus is very rare. The diagnosis of IMT base on histopathology and immunohistochemistry. The genesis and development of IMT result from chromosomal translocations that often cause an overexpression of anaplastic lymphoma kinase. IMT are clinical and pathological distinct entities and its biological behavior is still uncertain.
Adult ; Humans ; Male ; Maxillary Sinus Neoplasms ; pathology ; Neoplasms, Muscle Tissue ; pathology

Objective To investigate the effectiveness of the Weishule granule therapy with rabeprazole particles associ-ated chronic stomach .Methods 488 cases of chronic gastritis were divided into treatment group and control group .Control group:oral rabeprazole enteric-coated capsules ,1 time/d ,20 mg/times ;treatment groups :on the basis of additional services Weishule granules ,3 times/d ,10 g/times ,three weeks for a course respectively .The efficacy of two diseases ,endoscopy and TCM syndromes were compared .Results The treatment effect among functional dyspepsia ,gastritis treatment group and the control group had statistically difference (P<0.01);The treatment effect between atrophic gastritis treatment group and the control group was statistically difference (P<0.05);The efficacy of endoscopic lesions between the treatment group and the control group was statistically difference (P<0.05) .TCM syndrome ,the liver and stomach damp-heat syndrome ,Fan Wei qi syndrome treatment group and the control group ,had statistically difference (P<0.01) ,stomach meridian blood stasis ,spleen deficiency syndrome ,cold and heat mixture syndrome treatment group and the control group had statistically difference (P<0.05) .Conclusion The effective rate and cure rate of Weishule granule combined rabeprazole azole in patients with chronic stomach trouble was better with less adverse reactions .

Objective:To investigate the role of serum inflammatory cytokines in the development of acute coronary syndrome (ACS). Methods: All of enrolled patients were diagnosed by clinical and coronary angiographic features and divided into four groups, the acute myocardial infarction (AMI) group, unstable angina pectoris (UAP) group, stable angina pectoris (SAP) group and control group. The values of high-sersitivity C-reactive protein(hs-CRP), matrix metallopeptidase 9(MMP-9) and tumor necrosis factor-a (TNF-a) in serum were measured by cytokine detection equipment system (B10-RAD Biological Technology Co.Ltd, USA) and analysed in four groups with statistics. Results: Compared with SAP and the control groups, the levels of TNF-a and MMP-9 were increased significantly in AMI group(P <0.01). The level of serum hs-CRP was significantly higher in AMI group than that in UAP, SAP and control groups (P < 0.05). There were no differences in the levels of hs-CRP and MMP-9 between UAP, SAP and control groups (P> 0.05). It was found that there was positive relation between hs-CRP, MMP-9 and TNF-a by Pearson correlation analysis. Conclusion:There was obvious relation between coronary heart disease and inflammation. The cytokines characterized by the increases of hs-CRP, TNF-a and MMP-9 were involved in the formation and progression of atherosclerosis and served as markers of unstable plagues.