Objective:To explore the relationship between ten‐eleven translocation 2 (TET2) Mutations and Expression of 5hmC in breast cancer .Methods :TET2 mutations in breast cancer tissues from 100 patients were detected by Sanger method . The expression of 5hmC in breast cancer tissues was detected by immunohistochemistry . The relationship between TET2 mutations and expression of 5hmC was analyzed .Results :A total of 13 kinds of TET2 mutations were identified in 100 patients .Among them ,3 new types of mutations were identified in 4 patients . They were Ala341Val , Leu1622Phe and Pro1578Tyr .Pro1578Tyr was identified in two patients .And immunohistochemical analysis showed that there was a sharp reduction of 5hmC in breast cancer tissues with TET2 mutations than in breast cancer tissues without TET2 mutations . Conclusions :T ET2 mutations can lead to decrease of 5hmC level in breast cancer .

Objective To investigate the exposure level of radioactive hazard factors and the health management of radiation workers in non-medical radiation institutions (excluding military institutions) in Jinan, China through radioactive hazard factor monitoring, to identify the weak links, and to provide a scientific basis for future work priorities. Methods According to the monitoring plan formulated by Jinan Municipal Health Commission, the task undertaking institutions at all levels in Jinan investigated the types of radioactive hazard factors, detection, training, and health monitoring of 101 non-medical radiation institutions in Jinan. In addition, the workplace radiation levels were detected in 25 institutions of 6 types of monitoring objects, including industrial flaw detection, non-medical accelerator, non-sealed radioactive material workplace, nuclear instrument, baggage detector, and others. Results The investigation objects included institutions engaged in industrial flaw detection, nuclear instrument, luggage detector, non-medical accelerator, non-sealed source workplace, and others. Of these institutions, 91.84% were equipped with radiation protection detectors, 92.86% were equipped with personal dose alarm, 97.73% were equipped with personal protective equipment, 94.36% performed radiation protection training, 92.69% employed radiation workers with certificates, 95.77% performed personal dose detection, 94.83% performed occupational health examination, and 100.00% were qualified for radiation protection detection in workplace. Conclusion There is still a gap between the radiation protection status of non-medical institutions in Jinan and the national regulations and standards, so it is necessary to further strengthen supervision and law enforcement and make greater efforts in training and publicity.