Objective To establish a small amount of acid ,microwave digestion‐atomic fluorescence spectrometer method was developed for the determination of arsenic and mercury in eggs at the same time .Methods The sample was digested with 1 ml HNO3 ,After digestion ,the sample was deoxidized with sulfocarbamide and vitamin C and then determined by AFs without dispel‐ling the acid .Results The sample was digested with 1 mL HNO3 ,After digestion ,the sample was deoxidized with sulfocarbamide and vitamin C and then determined by AFs without dispelling the acid .Conclusion The method is simple ,rapid and with little pol‐lution .The determination of arsenic and mercury in eggs by Atomic Fluorescence Spectrometry using microwave digestion were de‐veloped with satisfactory sensitivity ,accurate and precision .

Carcinoma ex pleomorphic adenoma occurring in the sublingual gland is extremely rare. In this report, a case of adenoid cystic carcinoma ex pleomorphic adenoma of the sublingual gland was presented.
Adenocarcinoma ; Adenoma, Pleomorphic ; Humans ; Sublingual Gland ; Sublingual Gland Neoplasms

Objective To construct two DNA vaccines based on two glycoprotein antigen segments of Xinjiang hemorrhagic fever virus (XHFV) and to evaluate the immune responses in BALB/c mice following vaccination.Methods Two recombinant expression plasmids pVAX1-GcⅠand pVAX1-Gc Ⅱ were constructed by inserting XHFV YL04057 strain Gc Ⅰ (1 229-1 349 aa) and Gc Ⅱ (1 443-1 566 aa) fragments into the eukaryotic expression vector pVAX1 and then were identify by restriction enzyme digestion and sequencing analysis.The recombinant expression plasmids were transfected into mice by hydrodynamics-based transfection.Immune responses induced in mice were evaluated by testing the proliferation of T cells with MTT,measuring serum antibody level with ELISA and detecting cytokines in the supernatant of spleen cell culture with ELISA kit.Results The recombinant expression plasmids were successfully constructed as indicated by the results of restriction enzyme digestion and sequencing analysis.Expression of Gc Ⅰ and Gc Ⅱ genes in mice liver tissues was detected.Antibody titers in mice immunized with pVAX1-GcⅠor pVAX1-Gc Ⅱ were higher than those in mice immunized with pVAX1.Compared with pVAX1,pVAX1-Gc Ⅱ significantly enhanced the proliferation of splenic T lymphocytes and the expression of IFN-γ (P<0.01).Conclusion The constructed two DNA vaccines for XHFV can induce specific humoral and cellular immune responses in mice.pVAX1-Gc Ⅱ is better than pVAX1-GcⅠin immunogenicity and protective efficacy,suggesting that it can be used as a promising candidate for the development of DNA vaccine for XHFV.

OBJECTIVETo investigate the effects of light on baicalin,baicalein accumulation and related genes expression in suspension cultures of Scutellaria baicalensis.

METHODThe content of baicalin, baicalein in suspension cultures of S. baicalensis was determined by HPLC. The related genes expression was analyzed by semi-quantitative PCR.

RESULTActive ingredients in suspension cultures of S. baicalensis were promoted significantly by light, PAL gene transcription under the conditions of light was higher than that under the dark conditions, the UBGAT gene expression levels was significantly related to the baicalein (r = -0.995).

CONCLUSIONActive ingredients and related genes (PAL, UBGAT) in suspension cultures of S. baicalensis were promoted significantly by light.

Antioxidants ; pharmacology ; Cell Culture Techniques ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; pharmacology ; Flavanones ; analysis ; genetics ; radiation effects ; Flavonoids ; analysis ; genetics ; pharmacology ; radiation effects ; Free Radical Scavengers ; pharmacology ; Light ; Medicine, Chinese Traditional ; methods ; Phytotherapy ; methods ; Plant Extracts ; chemistry ; Rehmannia ; chemistry ; Scutellaria baicalensis ; chemistry ; growth & development ; Suspensions ; chemistry

Objective To compare the distribution and concentration of matrix metalloproteinases-2 (MMP-2) in different dentin depth of premolar and molar of young people.Methods Freshly extracted human premolars and molars (aged between 20-30) were sectioned to 1.5 mm thick slices along the longitudinal axis of the tooth separately.Enamel and pulp of each slice was removed,and then the premolar and molar slices were respectively divided into two subgroups according to superficial or deep dentin and pulverized to fine powder.After dentin protein was extracted,the concentrations of MMP-2 in different tooth were detected using fluorescent microsphere immunoassay.Results The content of MMP-2 in superficial layer dentin of premolar was (0.022 ± 0.006) ng/mg.The content of MMP-2 in deep layer dentin of premolar was (2.087 ± 0.090) ng/mg.The content of MMP-2 in superficial layer dentin of molar was (0.336 ± 0.037) ng/mg.The content of MMP-2 in deep layer dentin of molar was (3.312 ± 0.308) ng/mg.Conclusions MMP-2 exists in human coronal dentin.In the same type of teeth of young people,the concentration of MMP-2 in deep dentin was significant higher than those in superficial dentin.In the same dentin depth,the concentration of MMP-2 in molar was significant higher than those in premolar.

Objective To systematically investigate the aging effect of thermocycling,water storage and bacteria aggression on the stability of resin-dentin bonds.Methods Forty molars were sectioned perpendicularly to the axis of the teeth to expose the middle-coronal dentin surfaces.The dentin surfaces were then treated with Single Bond 2 and made a core build-up.According to random digits table,the bonding specimens were divided into four groups (n =10)as follows:immediate control group,aging group with thermocycling for 5 000 times,aging group with artificial saliva storage for 6 months and aging group with bacteria aggression for 14 days.The specimens in each group were then subjected to microtensile bond strengths (μTBS) testing and nanoleakage evaluation respectively.Results After aging treatments,the three aging groups showed significantly lower μTBS than the immediate control group[(44.24 ± 12.75) MPa,P ＜0.05].The immediate control group also showed the lowest value of nanoleakage.The μTBS of aging group with bacteria aggression[(25.53 ± 7.39) MPa] was significantly lower than those of the other aging groups with artificial saliva storage [(29.72 ± 6.51) MPa] and thermocycling [(31.92 ± 11.87) MPa,P ＜ 0.05].There were no differences in the nanoleakage values among the three aging groups(P ＞0.05).Conclusions All the aging treatments with artificial saliva storage,thermocycling and bacteria aggression could accelerate the degradation of bonding interfaces between an etch-and-rinse adhesive and dentin.Bacteria aggression showed the most impairing effect on the stability of resin-dentin bonds.

Objective: To study the influence of personality on the occurrence of dental trauma of school-aged children. Methods:The school-aged children with dental trauma who came to visit our department were randomly recruited. The Eysenck Personality Scale was used to test the subjects who met the inclusion criteria. Results: 306 children were included in the study. There was a statistically significant association between personality type and the number of traumatic teeth (P ＜ 0. 05) and the number of traumatic teeth in children with extroversion was higher than that of children with neutrality or introversion (P ＜ 0. 05) . Conclusion: Extroverted personality is a significant correlation factor affecting the severity of dental trauma in children, and it is of great significance to provide scientific guidance and warning for them to prevent tooth trauma.

Carcinoma ex pleomorphic adenoma occurring in the sublingual gland is extremely rare. In this report, a case of adenoid cystic carcinoma ex pleomorphic adenoma of the sublingual gland was presented.

Objective:To explore the relationship between ten‐eleven translocation 2 (TET2) Mutations and Expression of 5hmC in breast cancer .Methods :TET2 mutations in breast cancer tissues from 100 patients were detected by Sanger method . The expression of 5hmC in breast cancer tissues was detected by immunohistochemistry . The relationship between TET2 mutations and expression of 5hmC was analyzed .Results :A total of 13 kinds of TET2 mutations were identified in 100 patients .Among them ,3 new types of mutations were identified in 4 patients . They were Ala341Val , Leu1622Phe and Pro1578Tyr .Pro1578Tyr was identified in two patients .And immunohistochemical analysis showed that there was a sharp reduction of 5hmC in breast cancer tissues with TET2 mutations than in breast cancer tissues without TET2 mutations . Conclusions :T ET2 mutations can lead to decrease of 5hmC level in breast cancer .

Acute or chronic wounds are common clinical problems. Collagen, with advantages including rich sources, impeccable biocompatibility, and inherent biodegradability, has been widely used in fundamental research and clinical treatment of wound repair with broad prospects of clinical applications. This article provided a brief overview of the role of collagen in various biological processes related to wound healing and also outlined the sources of collagen. Furthermore, the article summarized the application and recent research advancements of collagen-based wound dressings in the field of wound repair.