1.Application of differentiation acupoint massage in empowerment education of the elderly with hypertension
Yujia MAO ; Xiangeng ZHANG ; Jijun WU ; Yufu YIN ; Chunying LI ; Peizhen XING
Chinese Journal of Practical Nursing 2013;29(26):1-3
Objective To evaluate the effectiveness of differentiation acupoint massage on the level of blood pressure control and self-efficacy in the elderly with hypertension with yin-deficiency and yangexcess under the model of empowerment education.Methods 80 elderly with hypertension with yin-deficiency and yang-excess were randomly divided into the intervention group and the control group with 40 patients in each group.Both of them received health education of differentiation acupoint massage consisted of 4 weekly sessions.The intervention group received it under the model of empowerment education whilst the other under the model of the general health education.After 4 weeks of sessions,all patients were followed up for 3 months.The blood pressure value was measured before the sessions and 4 weeks and 3 months after the sessions.The scores of self-efficacy was collected before and 3 months later.Results There were significant differences in the blood pressure,self-efficacy between the two groups after the intervention.Conclusions Differentiation acupoint massage under the model of empowerment education can significantly improve patients'self-efficacy and decrease the level of the blood pressure.
2.Observation of clinical effects of improved surgery on adolescent concomi-tant strabismus
Yuanyuan YANG ; Baoqin ZHANG ; Yujia MAO ; Lei ZHENG ; Minzhi ZHANG ; Dapeng TAN
China Modern Doctor 2015;(22):63-66
Objective To observe the application of improved Parks incision combined with proparacaine surface anes-thesia in the correction surgery of adolescent concomitant strabismus and the postoperative recovery situation. Methods Fifty-five patients who had received concomitant strabismus correction surgery in our hospital were randomly selected and divided into the improved group and the traditional group, of which the improved group (35 patients) was given the improved Parks incision combined with proparacaine surface anesthesia surgical method and the traditional group (20 patients) was given traditional corneal limbus trapezoid incision combined with lidocaine local anesthesia surgical method. The surgical anesthesia tolerance, postoperative cosmetic results and preoperative and postoperative tear film stability of the two groups were observed. The data were analyzed statistically. Results The patients received propara caine surface anesthesia and the patients received lidocaine local anesthesia were not significantly different in the toler-ance(P>0.05). Regarding to cosmetic results, the Parks incision concealed itself better than the traditional corneal limbus trapezoid incision, and showed faster and better recovery and better postoperative orthotopic effects, with statis-tically significant differences. The improved group was better than the traditional group in the fluorescein staining tear film break-up time (FBUT) and schirmer's Ⅰ experiment (S1T) 30 days after surgery, with statistically significant dif-ferences. Conclusion The improved surgery has better anesthetic tolerance effect, less invasive operation, less damage to normal tissue, better postoperative cosmetic results and smaller influence on ocular surface tear film function than the traditional surgery, thereby worthy of promotion.
3.Overexpression of miR-155-5p Inhibits the Proliferation and Migration of IL-13-Induced Human Bronchial Smooth Muscle Cells by Suppressing TGF-β-Activated Kinase 1/MAP3K7-Binding Protein 2.
Yujia SHI ; Xingli FU ; Qi CAO ; Zhengdao MAO ; Yi CHEN ; Yun SUN ; Zhiguang LIU ; Qian ZHANG
Allergy, Asthma & Immunology Research 2018;10(3):260-267
PURPOSE: Molecular mechanisms leading to asthma is still ill-defined. Though the function of microRNAs (miRNAs) in asthma was previously reported, the involvement of miR-155 in important features of this disease remains unknown. The present study was designed to uncover the probable involvement of miR-155-5p in the proliferation and migration of IL-13-induced human bronchial smooth muscle cells (BSMCs) and the intrinsic regulatory mechanism. METHODS: The effects of different concentrations of IL-13 on the proliferation and migration of BSMCs as well as the expression of miR-155-5p and its predicted target transforming growth factor (TGF)-β-activated kinase 1/MAP3K7-binding protein 2 (TAB2) were investigated. The effects of miR-155-5p on the proliferation and migration of interleukin (IL)-13-induced BSMCs was determined in vitro using BSMCs transfected with miR-155 mimic/inhibitor and induced by a high concentration of IL-13. The quantitative real-time polymerase chain reaction (qRTPCR) was employed for determining the expression of miR-155-5p and TAB2. Western blotting was applied to analyze the expression of TAB2 at the protein level. Cell proliferation and migration were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Transwell assays, respectively. RESULTS: The proliferation and migration of BSMCs were dose-dependently increased with IL-13 treatment. Contrariwise, IL-13 dose-dependently inhibited the expression of miR-155-5p in BSMCs. Mechanistic studies showed that inhibition of miR-155-5p further promoted the stimulatory effects of IL-13, whereas overexpression of miR-155 significantly inhibited these effects. In silico studies and luciferase reporter assays indicated that TAB2 was a negatively regulated miR-155-5p target. CONCLUSIONS: These results suggested that miR-155-5p-inhibit the IL-13-induced proliferation and migration of BSMCs by targeting TAB2 and that the IL-13/miR-155/TAB2 pathway could serve as a therapeutic target for pulmonary diseases, especially asthma.
Asthma
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Blotting, Western
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Cell Proliferation
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Computer Simulation
;
Humans*
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In Vitro Techniques
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Interleukin-13
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Interleukins
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Luciferases
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Lung Diseases
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MicroRNAs
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Muscle, Smooth*
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Myocytes, Smooth Muscle*
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Phosphotransferases*
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Real-Time Polymerase Chain Reaction
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Transforming Growth Factors
4.Emerging infectious diseases in voluntary blood donors in parts of China: Based on metagenomics analysis
Yuhui LI ; Zhan GAO ; Shilin LI ; Yujia LI ; Yang HUANG ; Limin CHEN ; Mei HUANG ; Jianhua WAN ; Weilan HE ; Wei MAO ; Jie CAI ; Jingyu ZHOU ; Ru YANG ; Yijing YIN ; Yanli GUO ; Miao HE
Chinese Journal of Blood Transfusion 2021;34(5):440-446
【Objective】 To analyze the metagenomics and microbiology of voluntary blood donors in China, so as to assess the potential threats of emerging infectious diseases to the safety of blood transfusion. 【Methods】 12 300 plasma samples (10 mL each) collected by central blood stations in Chongqing, Liuzhou, Urumqi, Mianyang, Wuhan, Nanjing, Mudanjiang, and Dehong Prefecture area from 2012 to 2018 were subjected to total DNA extraction after ultracentrifugation (32 000 rpm/min, centrifugal radius 91.9 mm) in minipools of 160 donations. The metagenomic library was constructed, and deep sequencing was conducted by Illumina Hiseq 4 500. By comparing with reference sequences of bacteria, fungi, parasites and viruses, metagenomic data were analyzed, classification of microbes were identified, and potentially harmful pathogens were evaluated. 【Results】 A total of 632 GB clean data were obtained by deep sequencing, and the top three pathogens were Pseudomonas(0.561 1%), Burkholderia(0.468 7%) and Serratia(4.242 0%). Pathogens with potential threat which could be transmitted by blood transfusion or blood products were found, such as human parvovirus B19(0.126 6%), Leishmania spp(1.348 5%) and Toxoplasma gondii(0.615 8%). 【Conclusion】 Our study analyzed metagenomics of voluntary blood donors in parts of China and revealed pathogens that may cause potential harm to blood safety, which were helpful for targeted prevention and control of emerging infectious diseases.
5.Gender differences in mortality following tanscatheter aortic valve replacement (TAVR): a single-centre retrospective analysis from China.
Qi LIU ; Yali WANG ; Yijian LI ; Tianyuan XIONG ; Fei CHEN ; Yuanweixiang OU ; Xi WANG ; Yijun YAO ; Kaiyu JIA ; Yujia LIANG ; Xin WEI ; Xi LI ; Yong PENG ; Jiafu WEI ; Sen HE ; Qiao LI ; Wei MENG ; Guo CHEN ; Wenxia ZHOU ; Mingxia ZHENG ; Xuan ZHOU ; Zhengang ZHAO ; Chen MAO ; Feng YUAN
Chinese Medical Journal 2023;136(20):2511-2513
6.Peri-procedural myocardial injury predicts poor short-term prognosis after TAVR: A single-center retrospective analysis from China.
Qi LIU ; Kaiyu JIA ; Yijun YAO ; Yijian LI ; Tianyuan XIONG ; Fei CHEN ; Yuanweixiang OU ; Xi WANG ; Yujia LIANG ; Xi LI ; Yong PENG ; Jiafu WEI ; Sen HE ; Qiao LI ; Wei MENG ; Guo CHEN ; Wenxia ZHOU ; Mingxia ZHENG ; Xuan ZHOU ; Yuan FENG ; Mao CHEN
Chinese Medical Journal 2023;136(24):3013-3015