OBJECTIVE:To study the bioequivalence of fluconazole in healthy volunteers.METHODS:By a randomized double crossover design,a total of 20 healthy male volunteers were assigned to receive oral single dose of test fluconazole tablets (300 mg) and reference fluconazole tablets(300 mg).The plasma concentrations of fluconazole at different time after oral ad- ministration were determined by HPLC,with pharmacokinetic parameters computed and the bioequivalence of the two formu- lations of fluconazole evaluated.RESULTS:The pharmacokinetic parameters of test fluconazole tablets vs.the reference flu- conazole tablets were as follows:t_(1～2):(29.98?4.79)hvs.(30.60?4.66)h,t_(max):(1.9?0.3)hvs.(1.9?0.6)h;C _(max):(7.64?0.98)?g?mL~(-1)vs.(7.93?0.78)?g?mL~(-1) AUC_(0～96):(258.41?36.49)?g?h?mL~(-1)vs.(267.93?31.24)?g?h?mL~(-1) AUC_(0～∞):(283.17?45.80)?g?h?mL~(-1)vs.(299.70?38.41)?g?h?mL~(-1) and derived from planimetry AUC_(0～96) and AUC_(0～∞),the relative bioavailability of the test tablet were(94.9?11.3)%and(94.8?11.9)%,respectively.CONCLUSION: The test fluconazole tablets and the reference fluconazole tablets were proved to be bioequivalent.

Objective To clone and express a novel gene cDNA fragment,pbmag-1,from Plasmodium berghei ANKA strain. Methods The cDNA sequence of pbmag-1 was obtained from the GenBank of P.berghei ANKA genomic databases,with which a pair of primers was designed and RT-PCR was used to get a cDNA fragment of the gene from the parasite. The expanded cDNA 3′ fragment of the gene was obtained by 3′-RACE using the oligo dT primer and a set of specific primers. The intact cDNA 3′ fragment was cloned into a prokaryotic expressional vector and transformed into the BL21-(DE3)-RIL strain of Escherichia coli. The recombinant protein of PbMAg-1 was expressed with an optimized strategy and used to immunize mice. Results The pbmag-1 cDNA fragment obtained was 1 341 bp in length,A/T rich (73%) and with a correct 3′ end sequence. By Western blot,the anti-serum of mice immunized with the recombinant protein of PbMAg-1/GST,which was expressed as inclusion bodies,specifically recognized a band with Mr 64 000 molecule from the protein extracts of P. berghei-infected mouse erythrocytes. Conclusion The pbmag-1 cDNA sequence with intact 3′ has been obtained,which will be used for further study on its role in the immune response of P. berghei infection.

Teacher portfolio is originally used for teacher assessment.The role of a teacher portfolio includes strengthening teaching ethics for improving faculty's quality of professional ethics,finding faculty's disadvantages and problems,enhancing faculty's training,providing the basis for faculty assessment,promotion and the appointment,and developing clinical teachers' teaching and research capacity.

Objective To evaluate the efficacy and safety of different doses Octreotide combined with Lansoprazole in the treatment of hepatocirrhosis with upper gastrointestinal bleeding.Methods Eighty hepatocirrhosis patients with upper gastrointestinal bleeding during May.2013 to May.2016 were treated with Octreotide combined with Lansoprazole,and were divided into high dose group (40 cases) and low dose group (40 cases) according to different doses Octreotide.Before and after treatment,portalvenousmaximumvelocity (PVV) and portalveindiameter (PVD) were detected by color Doppler ultrasound respectively,and haemostatic time,blood transfusion cases and volume,rehaemorrhagia cases were recorded,and adverse reactions were observed.Results After treatment,PVV and PVD of the two groups were significantly ameliorative (P ＜ 0.05),and there were no significantly different between two groups.Haemostatic time,blood transfusiorate,blood transfusiorate volume and rehaemorrhagia rate of the high dose group were significantly less than the low dose group (P ＜ 0.05).The clinical effective rate of the high dose group was significantly higher than that of the low dose group (P ＜ 0.05).The rate of adverse reactions of the high dose group was not significantly different from that of the low dose group.Conclusions High doses Octreotide combined with Lansoprazole in the treatment of hepatocirrhosis with upper gastrointestinal bleeding have a gain on hemostatic effect,and cannot increase adverse reactions significantly.

OBJECTIVE:To explore hospital pharmaceutical care mode to promote clinical rational drug use.METHODS:The consumption data of drugs for patients with cardiovascular and cerebrovascular diseases during 2006 in dispensary of traditional Chinese medicine of our hospital were investigated and analyzed.RESULTS:The ratio of drug combination increased with the rising of patients' age,so did the incidence of adverse drug reactions(ADR).CONCLUSION:Pharmacists' pharmaceutical care plays an important role in promoting patients' rational drug use and treatment.

Objective To find out the relativity of diseases and emotional factors,provide foundation for the clinical epidemiologic survey,and to know about the doctors understanding of emotional etiology.Method Consulting to the diagnostic criterion of emotional diseases,whole-team-delamination method was adopted to research 54 703 registered case history of retrospective etiology in hospitals(Established Hospital of Shandong Province,Jinan City Central Hospital,TCM Hospital of Shandong Province) within 3 years.Result The cases that was related to emotions were totally 506,0.92% of all the cases.Of all the positive cases,62.5% of them were cardicvascular diseases,12.06% were digestive diseases,3.56% were gynecologic diseases.Of all the emotional etiology,agitation was 43.87%,anger was 25.69%.Female patients took up 56.72% of all the positive cases.Conclusion Cardiovascular diseases are more related to negative emotions,the basic emotion"anger"is more related to diseases,female is prior to male patients,age is of positive correlation of the occurrence of emotional diseases.

Objective Aimed at the problem that there is no curative effect standard of liver-qi invasion of premenstrual syndrome (PMS), to construct the reference standard of curative effect evaluation for the liver-qi invasion of PMS. Method Curative effect evaluation research of Jingqianping granule was designed by the principle of random, double blind, contrast and multicentre. Three hundred and fifty-six patients with liver-qi invasion of PMS were divided into two groups randomly, one was Jingqianping granule, the other was placebo. Patients were given Jingqianping granule and placebo respectively for two menstrual cycles and followed–up for three menstrual cycles. The reference standard of curative effect evaluation for the liver-qi invasion of PMS was constructed with daily rating of severity of problems form (DRSP). Result Jingqianping granule and placebo have therapeutic effect on the patients with liver-qi invasion of PMS, and Jingqianping granule was better than placebo. DRSP can be used as indexes of curative effect evaluation. Conclusion The initial establishment of the curative effect evaluation criterion of PMS liver-qi invasion provides the important foundation for profession criterion’s establishment.

Objective To compare the efficacy and toxicity of late-course concurrent radiochemotherapy and sequential chemoradiotherapy for advanced esophageal carcinoma. Methods Eighty-two patients with advanced esophageal carcinoma were randomized into two groups: 41 cases in late-course concurrent radiochemotherapy (LCRC) group were received two cycles chemotherapy and then underwent concurrent radiochemotherapy;41 cases in sequential chemoradiotherapy (SCR) group were received four cycles chemotherapy and then underwent radiotherapy. The regimen of chemotherapy in all cases:cisplatin 25mg/m2, 1-3 d;calcium folinate (CF) 150 mg/m2, 1-5 d;fluorouracil 375 mg/m2, 1-5 d, 21 d was one cycle. All patients were received the three-dimensional conformal radiation therapy, the total dose of radiation was same as 64 Gy. Results The short-term response rate was 85.4%(35/41) in LCRC group and 65.9%(27/41) in SCR group, they had significant difference ( P<0.05). The rates of acute radiation esophagitis that need treatment was 90.2%(37/41) in LCRC group and 87.8%(36/41) in SCR group, there had no significant difference (P>0.05). The l year, 2 years, 3 years survival rate were 68.3%(28/41) and 65.9%(27/41), 56.1%(23/41) and 51.2%(21/41), 46.3%(19/41) and 36.6%(15/41) respectively,the median survival time were 30.0 months and 26.0 months, there had no significant difference ( P>0.05). Conclusion The short-term efficacy of advanced esophageal carcinoma could be improved by the late-course concurrent radiochemotherapy.

Objective To investigate the feasibility,operation design and postoperative rehabilitation in ankylosing spondylitis patients with bilateral hips and knees bony ankylosis,who were operated on the 4 joints during one hospitalization period. Method Two operations were performed in one hospitalization period in three ankylosing spondylitis patients on the 2 hip joints and 2 knee joints respectively.One case,the left hip replacement was operated in the first operation,and the right hip and the double knees were operated in the second operation;the other two cases,unilateral hip and knee were operated in two operations respectively.Patients began to contract their lower limbs’ muscles initiatively after narcosis.The limbs were put on the CPM,the hips and knees were bent and extended repeatedly on the third day after operations.The patients began to leave bed and learned to walk 2 weeks after operations.They could walk 100 steps with aids when they leaft hospital. Result In six-month follow-up,the range of hip motion(degree)were 105?~145?(average 135?);the range of knee motion(degree)was 45?~60?(average 54?).The hip’ function score adopted Harris score system:the average of 83 and were all good;the knee’ function score adopted American Association of Knee Surgery score system in 1989:all were excellent except a good one(left knee of first case,the score was 74/75). Conclusion Performing operations on the 2 bony ankylosis hips and 2 bony ankylosis knees in the ankylosing spondylitis patients in one hospitalization period can shorten the treatment cycle,release the distress,and lighten the economic load,meanwhile it is convenient for operation and postoperative rehabilitation.It is better to do the unilateral hip and knee in a single operation.