MicroRNA is a kind of endogenous non-coding of small molecules RNA.It has been confirmed that the microRNA play a role in innate immunity and adaptive immunity which are involved in sepsis.

Neoadjuvant therapy aiming at improving the resection rate and prognosis for patients with lung cancer is a hot spot in the study field of lung cancer in today and future.This paper reviews the safety and clinical significance of neoadjuvant therapy for stage Ⅲ non-small-cell lung cancer.

OBJECTIVE:To study the bioequivalence of fluconazole in healthy volunteers.METHODS:By a randomized double crossover design,a total of 20 healthy male volunteers were assigned to receive oral single dose of test fluconazole tablets (300 mg) and reference fluconazole tablets(300 mg).The plasma concentrations of fluconazole at different time after oral ad- ministration were determined by HPLC,with pharmacokinetic parameters computed and the bioequivalence of the two formu- lations of fluconazole evaluated.RESULTS:The pharmacokinetic parameters of test fluconazole tablets vs.the reference flu- conazole tablets were as follows:t_(1～2):(29.98?4.79)hvs.(30.60?4.66)h,t_(max):(1.9?0.3)hvs.(1.9?0.6)h;C _(max):(7.64?0.98)?g?mL~(-1)vs.(7.93?0.78)?g?mL~(-1) AUC_(0～96):(258.41?36.49)?g?h?mL~(-1)vs.(267.93?31.24)?g?h?mL~(-1) AUC_(0～∞):(283.17?45.80)?g?h?mL~(-1)vs.(299.70?38.41)?g?h?mL~(-1) and derived from planimetry AUC_(0～96) and AUC_(0～∞),the relative bioavailability of the test tablet were(94.9?11.3)%and(94.8?11.9)%,respectively.CONCLUSION: The test fluconazole tablets and the reference fluconazole tablets were proved to be bioequivalent.

Objective To observe the therapeutic effect of treating cervical vertigo by needling acupoint Baihui (DU-20).Methods:Two groups were setup for observation.One group served as a treatment group and was treated by needling Baihui (DU-20);the other group served as a control group and was treated by western medicine.Results After being treated for two therapeutic courses,the cured rate was 34.92% in the treatment group and 17.46% in the control group,with a significant difference between the two group (P＜0.05) .Conclusion Needling acupoint baihui (DU-20) is an ideal therapy with a short therapeutic course,no adverse effect and reliable therapeutic effect for cervical vertigo.

Fluorouracil,oxaliplatin and irinotecan、are generally accepted as the standard chemothera-peutic agents for metastatic colorectal cancer.In recent years,the research advances on metastatic colorectal cancer treatment focus on the combination between different active drugs,and try to search advisable combined therapy methods.Clinical trials suggest that ahemating regimen is effective and has lower toxicity which provides platform for further study of combined chemotherapy for metastatic colorectal cancer.

Objective To observe the effect of antipruritic lotion combined Fuyanxiao with biological dress-ing suppository in the treatment of vulvovaginal candidiasis in pregnancy(VVC)the clinical curative effect and the recurrence rate.Methods 236 patients with pregnancy complicated with VVC,would be in community were randomly divided into the two groups:combination treatment group,single drug group,118 cases in each.Combination therapy using antipruritic lotion combined Fuyanxiao biological dressing suppository combined treatment,the control group was treated by Fuyanxiao biological dressing suppository treatment,two groups of clinical efficacy and recurrence rate after the end of treatment were compared.Results The cure rate in the combined treatment group was 90.68%,which was higher than 57.62 % in the control group(χ2 =10.731,P <0.01);The recurrence rate in the combined treatment group was 3.39%,which was lower than 13.56% in the control group(χ2 =10.733,P <0.05).Conclusion The antipruritic lotion combined Fuyanxiao biological dressing suppository in the treatment of pregnancy with VVC can effectively improve the clinical symptoms of patients,reduce the recurrence rate.

Yin injury is a common pathological in epidemic febrile disease, nourishing yin method is one of the commonly used therapies. This article initially summarized and discussed the heterogeneous mutual enhancement compatibility for nourishing yin in Detailed Analysis of Epidemic Warm Diseases, it could be refered for clinic.

Basic cognition of TCM for human body includes three aspects:(1)Human body is an unify of form,Qi and sprit intrinsically;(2)Human is an unity composed of five solid organs in functions and structures;(3)Form,Qi,sprit and the Zang-and Fu-organs of human body connect together via channels and collaterals.The characteristics of TCM study methods are mainly trialistic nature of study objects,phenomenon analogy,functional leading nature of function and unify of the whole.

ObjectiveTo achieve efficient expression of human serum albumin(HSA)in Chinese hamster ovary(CHO)cells and optimize its culture technology,so as to lay a foundation of the large-scale production of HSA.MethodsThe eukaryotic expression vector of HSA was constructed by gene recombination technology,and then electrotransfected into fully suspended CHO cells. The monoclonal cell lines with stable and high expression of HSA were screened by G418 and limited dilution method. By adding glucose,sodium butyrate and supplementalmedium to the basal medium,the cell culture process was optimized to improve the expression of HSA. Finally,the scale-up culture verification was carried out in a 5 L bioreactor.ResultsThe recombinant expression vector pcDNA3.1-HSA was successfully constructed and expressed in fully suspended CHO cells. After two monoclonal screening,the secondary monoclonal cell lines CHO-rHSA-7H2A9 and CHOrHSA-7H2D12 were obtained with high HSA expression of 29. 37 mg/L and 25. 26 mg/L respectively. The HSA expression level reached about 100. 00 mg/L by optimizing the culture process and wasfinally increased to 166. 16 mg/L in the 5 L bioreactor,which was about 30 times higher than that in the supernatant of the first monoclonal cells.Conclusion The high level expression of HSA in CHO cells was achieved,which laid a foundation of the further large-scale production of HSA in the field of biological products and solving the market supply problems.

SUMMARY Peripheral nerve defects are still a major challenge in clinical practice,and the most com-monly used method of treatment for peripheral nerve defects is nerve transplantation,which has certain limitations and shortcomings,so new repair methods and techniques are needed.The peripheral nerve is elongated in limb lengthening surgery without injury,from which we got inspirations and proposed a new method to repair peripheral nerve defects:peripheral nerve elongation.The peripheral nerve could be elongated by a certain percent,but the physiological change and the maximum elongation range were still unknown.This study discussed the endurance,the physiological and pathological change of peripheral nerve elongation in detail,and got a lot of useful data.First,we developed peripheral nerve extender which could match the slow and even extension of peripheral nerve.Then,our animal experiment result confirmed that the peripheral nerve had better endurance for chronic elongation than that of acute elonga-tion and cleared the extensibility of peripheral nerve and the range of repair for peripheral nerve defects. Our result also revealed the histological basis and changed the rule for pathological physiology of peri-pheral nerve elongation:the most important structure foundation of peripheral nerve elongation was Fon-tana band,which was the coiling of nerve fibers under the epineurium,so peripheral nerve could be stretched for 8.5% -10.0% without injury because of the Fontana band.We confirmed that peripheral nerve extending technology could have the same repair effect as traditional nerve transplantation through animal experiments.Finally,we compared the clinical outcomes between nerve elongation and perfor-mance of the conventional method in the repair of short-distance transection injuries in human elbows, and the post-operative follow-up results demonstrated that early neurological function recovery was better in the nerve elongation group than in the conventional group.On the whole,all of these experimental re-sults revealed the physiological phenomenon of peripheral nerve elongation,and described the physiologi-cal change and stretch range in detail.The systematic research results have filled the blank in this field, which is very helpful for clinical limb lengthening surgery,the design of elongation surgery and the evalu-ation of the peripheral nerve stretch injury.Peripheral nerve elongation will become an innovative treat-ment technology in repairing peripheral nerve defects.